Job ID = 1292886 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 32,287,963 reads read : 64,575,926 reads written : 64,575,926 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:19 32287963 reads; of these: 32287963 (100.00%) were paired; of these: 1774055 (5.49%) aligned concordantly 0 times 26028316 (80.61%) aligned concordantly exactly 1 time 4485592 (13.89%) aligned concordantly >1 times ---- 1774055 pairs aligned concordantly 0 times; of these: 1053406 (59.38%) aligned discordantly 1 time ---- 720649 pairs aligned 0 times concordantly or discordantly; of these: 1441298 mates make up the pairs; of these: 727725 (50.49%) aligned 0 times 421610 (29.25%) aligned exactly 1 time 291963 (20.26%) aligned >1 times 98.87% overall alignment rate Time searching: 00:35:19 Overall time: 00:35:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 587888 / 31545601 = 0.0186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:10:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:10:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:10:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:10:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:10:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:10:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:10:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:10:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:10:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:10:19: 1000000 INFO @ Sun, 02 Jun 2019 22:10:20: 1000000 INFO @ Sun, 02 Jun 2019 22:10:20: 1000000 INFO @ Sun, 02 Jun 2019 22:10:26: 2000000 INFO @ Sun, 02 Jun 2019 22:10:27: 2000000 INFO @ Sun, 02 Jun 2019 22:10:27: 2000000 INFO @ Sun, 02 Jun 2019 22:10:32: 3000000 INFO @ Sun, 02 Jun 2019 22:10:35: 3000000 INFO @ Sun, 02 Jun 2019 22:10:35: 3000000 INFO @ Sun, 02 Jun 2019 22:10:39: 4000000 INFO @ Sun, 02 Jun 2019 22:10:42: 4000000 INFO @ Sun, 02 Jun 2019 22:10:42: 4000000 INFO @ Sun, 02 Jun 2019 22:10:46: 5000000 INFO @ Sun, 02 Jun 2019 22:10:50: 5000000 INFO @ Sun, 02 Jun 2019 22:10:50: 5000000 INFO @ Sun, 02 Jun 2019 22:10:52: 6000000 INFO @ Sun, 02 Jun 2019 22:10:58: 6000000 INFO @ Sun, 02 Jun 2019 22:10:58: 6000000 INFO @ Sun, 02 Jun 2019 22:10:59: 7000000 INFO @ Sun, 02 Jun 2019 22:11:06: 8000000 INFO @ Sun, 02 Jun 2019 22:11:06: 7000000 INFO @ Sun, 02 Jun 2019 22:11:06: 7000000 INFO @ Sun, 02 Jun 2019 22:11:13: 9000000 INFO @ Sun, 02 Jun 2019 22:11:14: 8000000 INFO @ Sun, 02 Jun 2019 22:11:14: 8000000 INFO @ Sun, 02 Jun 2019 22:11:19: 10000000 INFO @ Sun, 02 Jun 2019 22:11:21: 9000000 INFO @ Sun, 02 Jun 2019 22:11:21: 9000000 INFO @ Sun, 02 Jun 2019 22:11:26: 11000000 INFO @ Sun, 02 Jun 2019 22:11:29: 10000000 INFO @ Sun, 02 Jun 2019 22:11:29: 10000000 INFO @ Sun, 02 Jun 2019 22:11:33: 12000000 INFO @ Sun, 02 Jun 2019 22:11:37: 11000000 INFO @ Sun, 02 Jun 2019 22:11:37: 11000000 INFO @ Sun, 02 Jun 2019 22:11:40: 13000000 INFO @ Sun, 02 Jun 2019 22:11:45: 12000000 INFO @ Sun, 02 Jun 2019 22:11:45: 12000000 INFO @ Sun, 02 Jun 2019 22:11:46: 14000000 INFO @ Sun, 02 Jun 2019 22:11:53: 13000000 INFO @ Sun, 02 Jun 2019 22:11:53: 15000000 INFO @ Sun, 02 Jun 2019 22:11:53: 13000000 INFO @ Sun, 02 Jun 2019 22:12:00: 16000000 INFO @ Sun, 02 Jun 2019 22:12:00: 14000000 INFO @ Sun, 02 Jun 2019 22:12:01: 14000000 INFO @ Sun, 02 Jun 2019 22:12:06: 17000000 INFO @ Sun, 02 Jun 2019 22:12:07: 15000000 INFO @ Sun, 02 Jun 2019 22:12:08: 15000000 INFO @ Sun, 02 Jun 2019 22:12:13: 18000000 INFO @ Sun, 02 Jun 2019 22:12:15: 16000000 INFO @ Sun, 02 Jun 2019 22:12:16: 16000000 INFO @ Sun, 02 Jun 2019 22:12:20: 19000000 INFO @ Sun, 02 Jun 2019 22:12:22: 17000000 INFO @ Sun, 02 Jun 2019 22:12:23: 17000000 INFO @ Sun, 02 Jun 2019 22:12:26: 20000000 INFO @ Sun, 02 Jun 2019 22:12:29: 18000000 INFO @ Sun, 02 Jun 2019 22:12:30: 18000000 INFO @ Sun, 02 Jun 2019 22:12:34: 21000000 INFO @ Sun, 02 Jun 2019 22:12:36: 19000000 INFO @ Sun, 02 Jun 2019 22:12:37: 19000000 INFO @ Sun, 02 Jun 2019 22:12:41: 22000000 INFO @ Sun, 02 Jun 2019 22:12:43: 20000000 INFO @ Sun, 02 Jun 2019 22:12:44: 20000000 INFO @ Sun, 02 Jun 2019 22:12:48: 23000000 INFO @ Sun, 02 Jun 2019 22:12:51: 21000000 INFO @ Sun, 02 Jun 2019 22:12:53: 21000000 INFO @ Sun, 02 Jun 2019 22:12:54: 24000000 INFO @ Sun, 02 Jun 2019 22:12:58: 22000000 INFO @ Sun, 02 Jun 2019 22:13:00: 22000000 INFO @ Sun, 02 Jun 2019 22:13:01: 25000000 INFO @ Sun, 02 Jun 2019 22:13:06: 23000000 INFO @ Sun, 02 Jun 2019 22:13:07: 23000000 INFO @ Sun, 02 Jun 2019 22:13:07: 26000000 INFO @ Sun, 02 Jun 2019 22:13:13: 24000000 INFO @ Sun, 02 Jun 2019 22:13:14: 24000000 INFO @ Sun, 02 Jun 2019 22:13:14: 27000000 INFO @ Sun, 02 Jun 2019 22:13:20: 25000000 INFO @ Sun, 02 Jun 2019 22:13:20: 28000000 INFO @ Sun, 02 Jun 2019 22:13:21: 25000000 INFO @ Sun, 02 Jun 2019 22:13:27: 26000000 INFO @ Sun, 02 Jun 2019 22:13:27: 29000000 INFO @ Sun, 02 Jun 2019 22:13:28: 26000000 INFO @ Sun, 02 Jun 2019 22:13:34: 30000000 INFO @ Sun, 02 Jun 2019 22:13:35: 27000000 INFO @ Sun, 02 Jun 2019 22:13:36: 27000000 INFO @ Sun, 02 Jun 2019 22:13:41: 31000000 INFO @ Sun, 02 Jun 2019 22:13:42: 28000000 INFO @ Sun, 02 Jun 2019 22:13:43: 28000000 INFO @ Sun, 02 Jun 2019 22:13:47: 32000000 INFO @ Sun, 02 Jun 2019 22:13:49: 29000000 INFO @ Sun, 02 Jun 2019 22:13:50: 29000000 INFO @ Sun, 02 Jun 2019 22:13:54: 33000000 INFO @ Sun, 02 Jun 2019 22:13:56: 30000000 INFO @ Sun, 02 Jun 2019 22:13:57: 30000000 INFO @ Sun, 02 Jun 2019 22:14:00: 34000000 INFO @ Sun, 02 Jun 2019 22:14:03: 31000000 INFO @ Sun, 02 Jun 2019 22:14:05: 31000000 INFO @ Sun, 02 Jun 2019 22:14:07: 35000000 INFO @ Sun, 02 Jun 2019 22:14:11: 32000000 INFO @ Sun, 02 Jun 2019 22:14:12: 32000000 INFO @ Sun, 02 Jun 2019 22:14:13: 36000000 INFO @ Sun, 02 Jun 2019 22:14:18: 33000000 INFO @ Sun, 02 Jun 2019 22:14:19: 33000000 INFO @ Sun, 02 Jun 2019 22:14:19: 37000000 INFO @ Sun, 02 Jun 2019 22:14:25: 34000000 INFO @ Sun, 02 Jun 2019 22:14:26: 38000000 INFO @ Sun, 02 Jun 2019 22:14:26: 34000000 INFO @ Sun, 02 Jun 2019 22:14:32: 39000000 INFO @ Sun, 02 Jun 2019 22:14:32: 35000000 INFO @ Sun, 02 Jun 2019 22:14:33: 35000000 INFO @ Sun, 02 Jun 2019 22:14:38: 40000000 INFO @ Sun, 02 Jun 2019 22:14:39: 36000000 INFO @ Sun, 02 Jun 2019 22:14:40: 36000000 INFO @ Sun, 02 Jun 2019 22:14:45: 41000000 INFO @ Sun, 02 Jun 2019 22:14:46: 37000000 INFO @ Sun, 02 Jun 2019 22:14:47: 37000000 INFO @ Sun, 02 Jun 2019 22:14:51: 42000000 INFO @ Sun, 02 Jun 2019 22:14:53: 38000000 INFO @ Sun, 02 Jun 2019 22:14:54: 38000000 INFO @ Sun, 02 Jun 2019 22:14:57: 43000000 INFO @ Sun, 02 Jun 2019 22:15:00: 39000000 INFO @ Sun, 02 Jun 2019 22:15:02: 39000000 INFO @ Sun, 02 Jun 2019 22:15:04: 44000000 INFO @ Sun, 02 Jun 2019 22:15:08: 40000000 INFO @ Sun, 02 Jun 2019 22:15:09: 40000000 INFO @ Sun, 02 Jun 2019 22:15:10: 45000000 INFO @ Sun, 02 Jun 2019 22:15:15: 41000000 INFO @ Sun, 02 Jun 2019 22:15:17: 41000000 INFO @ Sun, 02 Jun 2019 22:15:17: 46000000 INFO @ Sun, 02 Jun 2019 22:15:22: 42000000 INFO @ Sun, 02 Jun 2019 22:15:23: 47000000 INFO @ Sun, 02 Jun 2019 22:15:24: 42000000 INFO @ Sun, 02 Jun 2019 22:15:29: 43000000 INFO @ Sun, 02 Jun 2019 22:15:29: 48000000 INFO @ Sun, 02 Jun 2019 22:15:31: 43000000 INFO @ Sun, 02 Jun 2019 22:15:36: 49000000 INFO @ Sun, 02 Jun 2019 22:15:37: 44000000 INFO @ Sun, 02 Jun 2019 22:15:39: 44000000 INFO @ Sun, 02 Jun 2019 22:15:42: 50000000 INFO @ Sun, 02 Jun 2019 22:15:44: 45000000 INFO @ Sun, 02 Jun 2019 22:15:46: 45000000 INFO @ Sun, 02 Jun 2019 22:15:48: 51000000 INFO @ Sun, 02 Jun 2019 22:15:51: 46000000 INFO @ Sun, 02 Jun 2019 22:15:53: 46000000 INFO @ Sun, 02 Jun 2019 22:15:55: 52000000 INFO @ Sun, 02 Jun 2019 22:15:58: 47000000 INFO @ Sun, 02 Jun 2019 22:16:00: 47000000 INFO @ Sun, 02 Jun 2019 22:16:01: 53000000 INFO @ Sun, 02 Jun 2019 22:16:05: 48000000 INFO @ Sun, 02 Jun 2019 22:16:07: 48000000 INFO @ Sun, 02 Jun 2019 22:16:07: 54000000 INFO @ Sun, 02 Jun 2019 22:16:12: 49000000 INFO @ Sun, 02 Jun 2019 22:16:14: 55000000 INFO @ Sun, 02 Jun 2019 22:16:14: 49000000 INFO @ Sun, 02 Jun 2019 22:16:19: 50000000 INFO @ Sun, 02 Jun 2019 22:16:20: 56000000 INFO @ Sun, 02 Jun 2019 22:16:21: 50000000 INFO @ Sun, 02 Jun 2019 22:16:26: 57000000 INFO @ Sun, 02 Jun 2019 22:16:26: 51000000 INFO @ Sun, 02 Jun 2019 22:16:28: 51000000 INFO @ Sun, 02 Jun 2019 22:16:32: 58000000 INFO @ Sun, 02 Jun 2019 22:16:33: 52000000 INFO @ Sun, 02 Jun 2019 22:16:35: 52000000 INFO @ Sun, 02 Jun 2019 22:16:39: 59000000 INFO @ Sun, 02 Jun 2019 22:16:41: 53000000 INFO @ Sun, 02 Jun 2019 22:16:42: 53000000 INFO @ Sun, 02 Jun 2019 22:16:45: 60000000 INFO @ Sun, 02 Jun 2019 22:16:47: 54000000 INFO @ Sun, 02 Jun 2019 22:16:49: 54000000 INFO @ Sun, 02 Jun 2019 22:16:51: 61000000 INFO @ Sun, 02 Jun 2019 22:16:55: 55000000 INFO @ Sun, 02 Jun 2019 22:16:56: 55000000 INFO @ Sun, 02 Jun 2019 22:16:58: 62000000 INFO @ Sun, 02 Jun 2019 22:17:01: 56000000 INFO @ Sun, 02 Jun 2019 22:17:02: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:17:02: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:17:02: #1 total tags in treatment: 29932760 INFO @ Sun, 02 Jun 2019 22:17:02: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:17:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:17:03: #1 tags after filtering in treatment: 26061782 INFO @ Sun, 02 Jun 2019 22:17:03: #1 Redundant rate of treatment: 0.13 INFO @ Sun, 02 Jun 2019 22:17:03: #1 finished! INFO @ Sun, 02 Jun 2019 22:17:03: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:17:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:17:03: 56000000 INFO @ Sun, 02 Jun 2019 22:17:05: #2 number of paired peaks: 174 WARNING @ Sun, 02 Jun 2019 22:17:05: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Sun, 02 Jun 2019 22:17:05: start model_add_line... INFO @ Sun, 02 Jun 2019 22:17:05: start X-correlation... INFO @ Sun, 02 Jun 2019 22:17:05: end of X-cor INFO @ Sun, 02 Jun 2019 22:17:05: #2 finished! INFO @ Sun, 02 Jun 2019 22:17:05: #2 predicted fragment length is 51 bps INFO @ Sun, 02 Jun 2019 22:17:05: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Sun, 02 Jun 2019 22:17:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05_model.r WARNING @ Sun, 02 Jun 2019 22:17:05: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 22:17:05: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Sun, 02 Jun 2019 22:17:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 22:17:05: #3 Call peaks... INFO @ Sun, 02 Jun 2019 22:17:05: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 22:17:08: 57000000 INFO @ Sun, 02 Jun 2019 22:17:10: 57000000 INFO @ Sun, 02 Jun 2019 22:17:15: 58000000 INFO @ Sun, 02 Jun 2019 22:17:17: 58000000 INFO @ Sun, 02 Jun 2019 22:17:22: 59000000 INFO @ Sun, 02 Jun 2019 22:17:24: 59000000 INFO @ Sun, 02 Jun 2019 22:17:29: 60000000 INFO @ Sun, 02 Jun 2019 22:17:31: 60000000 INFO @ Sun, 02 Jun 2019 22:17:36: 61000000 INFO @ Sun, 02 Jun 2019 22:17:38: 61000000 INFO @ Sun, 02 Jun 2019 22:17:43: 62000000 INFO @ Sun, 02 Jun 2019 22:17:46: 62000000 INFO @ Sun, 02 Jun 2019 22:17:49: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:17:49: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:17:49: #1 total tags in treatment: 29932760 INFO @ Sun, 02 Jun 2019 22:17:49: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:17:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:17:49: #1 tags after filtering in treatment: 26061782 INFO @ Sun, 02 Jun 2019 22:17:49: #1 Redundant rate of treatment: 0.13 INFO @ Sun, 02 Jun 2019 22:17:49: #1 finished! INFO @ Sun, 02 Jun 2019 22:17:49: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:17:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:17:51: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:17:51: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:17:51: #1 total tags in treatment: 29932760 INFO @ Sun, 02 Jun 2019 22:17:51: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:17:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:17:51: #1 tags after filtering in treatment: 26061782 INFO @ Sun, 02 Jun 2019 22:17:51: #1 Redundant rate of treatment: 0.13 INFO @ Sun, 02 Jun 2019 22:17:51: #1 finished! INFO @ Sun, 02 Jun 2019 22:17:51: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:17:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:17:52: #2 number of paired peaks: 174 WARNING @ Sun, 02 Jun 2019 22:17:52: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Sun, 02 Jun 2019 22:17:52: start model_add_line... INFO @ Sun, 02 Jun 2019 22:17:52: start X-correlation... INFO @ Sun, 02 Jun 2019 22:17:52: end of X-cor INFO @ Sun, 02 Jun 2019 22:17:52: #2 finished! INFO @ Sun, 02 Jun 2019 22:17:52: #2 predicted fragment length is 51 bps INFO @ Sun, 02 Jun 2019 22:17:52: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Sun, 02 Jun 2019 22:17:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10_model.r WARNING @ Sun, 02 Jun 2019 22:17:52: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 22:17:52: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Sun, 02 Jun 2019 22:17:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 22:17:52: #3 Call peaks... INFO @ Sun, 02 Jun 2019 22:17:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 22:17:54: #2 number of paired peaks: 174 WARNING @ Sun, 02 Jun 2019 22:17:54: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Sun, 02 Jun 2019 22:17:54: start model_add_line... INFO @ Sun, 02 Jun 2019 22:17:54: start X-correlation... INFO @ Sun, 02 Jun 2019 22:17:54: end of X-cor INFO @ Sun, 02 Jun 2019 22:17:54: #2 finished! INFO @ Sun, 02 Jun 2019 22:17:54: #2 predicted fragment length is 51 bps INFO @ Sun, 02 Jun 2019 22:17:54: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Sun, 02 Jun 2019 22:17:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20_model.r WARNING @ Sun, 02 Jun 2019 22:17:54: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 22:17:54: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Sun, 02 Jun 2019 22:17:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 22:17:54: #3 Call peaks... INFO @ Sun, 02 Jun 2019 22:17:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 22:17:59: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 22:18:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05_peaks.xls INFO @ Sun, 02 Jun 2019 22:18:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 22:18:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.05_summits.bed INFO @ Sun, 02 Jun 2019 22:18:24: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (668 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:18:46: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 22:18:48: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 22:19:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10_peaks.xls INFO @ Sun, 02 Jun 2019 22:19:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 22:19:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.10_summits.bed INFO @ Sun, 02 Jun 2019 22:19:11: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (404 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:19:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20_peaks.xls INFO @ Sun, 02 Jun 2019 22:19:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 22:19:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4996816/SRX4996816.20_summits.bed INFO @ Sun, 02 Jun 2019 22:19:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (133 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。