Job ID = 1292838 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,809,537 reads read : 13,809,537 reads written : 13,809,537 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:25 13809537 reads; of these: 13809537 (100.00%) were unpaired; of these: 2269443 (16.43%) aligned 0 times 9709624 (70.31%) aligned exactly 1 time 1830470 (13.26%) aligned >1 times 83.57% overall alignment rate Time searching: 00:02:25 Overall time: 00:02:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1987903 / 11540094 = 0.1723 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 20:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:49:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:49:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:49:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:49:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:49:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:49:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:49:32: 1000000 INFO @ Sun, 02 Jun 2019 20:49:33: 1000000 INFO @ Sun, 02 Jun 2019 20:49:33: 1000000 INFO @ Sun, 02 Jun 2019 20:49:39: 2000000 INFO @ Sun, 02 Jun 2019 20:49:40: 2000000 INFO @ Sun, 02 Jun 2019 20:49:40: 2000000 INFO @ Sun, 02 Jun 2019 20:49:46: 3000000 INFO @ Sun, 02 Jun 2019 20:49:47: 3000000 INFO @ Sun, 02 Jun 2019 20:49:47: 3000000 INFO @ Sun, 02 Jun 2019 20:49:52: 4000000 INFO @ Sun, 02 Jun 2019 20:49:54: 4000000 INFO @ Sun, 02 Jun 2019 20:49:54: 4000000 INFO @ Sun, 02 Jun 2019 20:49:59: 5000000 INFO @ Sun, 02 Jun 2019 20:50:01: 5000000 INFO @ Sun, 02 Jun 2019 20:50:01: 5000000 INFO @ Sun, 02 Jun 2019 20:50:06: 6000000 INFO @ Sun, 02 Jun 2019 20:50:08: 6000000 INFO @ Sun, 02 Jun 2019 20:50:08: 6000000 INFO @ Sun, 02 Jun 2019 20:50:12: 7000000 INFO @ Sun, 02 Jun 2019 20:50:14: 7000000 INFO @ Sun, 02 Jun 2019 20:50:14: 7000000 INFO @ Sun, 02 Jun 2019 20:50:20: 8000000 INFO @ Sun, 02 Jun 2019 20:50:21: 8000000 INFO @ Sun, 02 Jun 2019 20:50:21: 8000000 INFO @ Sun, 02 Jun 2019 20:50:27: 9000000 INFO @ Sun, 02 Jun 2019 20:50:28: 9000000 INFO @ Sun, 02 Jun 2019 20:50:28: 9000000 INFO @ Sun, 02 Jun 2019 20:50:31: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:50:31: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:50:31: #1 total tags in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:31: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:50:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:50:32: #1 tags after filtering in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:50:32: #1 finished! INFO @ Sun, 02 Jun 2019 20:50:32: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:50:32: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:50:32: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:50:32: #1 total tags in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:32: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:50:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:50:32: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:50:32: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:50:32: #1 total tags in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:32: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:50:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:50:32: #1 tags after filtering in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:50:32: #1 finished! INFO @ Sun, 02 Jun 2019 20:50:32: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:50:32: #1 tags after filtering in treatment: 9552191 INFO @ Sun, 02 Jun 2019 20:50:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:50:32: #1 finished! INFO @ Sun, 02 Jun 2019 20:50:32: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:50:32: #2 number of paired peaks: 292 WARNING @ Sun, 02 Jun 2019 20:50:32: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Sun, 02 Jun 2019 20:50:32: start model_add_line... INFO @ Sun, 02 Jun 2019 20:50:33: start X-correlation... INFO @ Sun, 02 Jun 2019 20:50:33: end of X-cor INFO @ Sun, 02 Jun 2019 20:50:33: #2 finished! INFO @ Sun, 02 Jun 2019 20:50:33: #2 predicted fragment length is 39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2 alternative fragment length(s) may be 2,39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05_model.r WARNING @ Sun, 02 Jun 2019 20:50:33: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You may need to consider one of the other alternative d(s): 2,39 WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:50:33: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:50:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 20:50:33: #2 number of paired peaks: 292 WARNING @ Sun, 02 Jun 2019 20:50:33: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Sun, 02 Jun 2019 20:50:33: #2 number of paired peaks: 292 INFO @ Sun, 02 Jun 2019 20:50:33: start model_add_line... WARNING @ Sun, 02 Jun 2019 20:50:33: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Sun, 02 Jun 2019 20:50:33: start model_add_line... INFO @ Sun, 02 Jun 2019 20:50:33: start X-correlation... INFO @ Sun, 02 Jun 2019 20:50:33: start X-correlation... INFO @ Sun, 02 Jun 2019 20:50:33: end of X-cor INFO @ Sun, 02 Jun 2019 20:50:33: #2 finished! INFO @ Sun, 02 Jun 2019 20:50:33: #2 predicted fragment length is 39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2 alternative fragment length(s) may be 2,39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20_model.r INFO @ Sun, 02 Jun 2019 20:50:33: end of X-cor INFO @ Sun, 02 Jun 2019 20:50:33: #2 finished! INFO @ Sun, 02 Jun 2019 20:50:33: #2 predicted fragment length is 39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2 alternative fragment length(s) may be 2,39 bps INFO @ Sun, 02 Jun 2019 20:50:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10_model.r WARNING @ Sun, 02 Jun 2019 20:50:33: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You may need to consider one of the other alternative d(s): 2,39 WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:50:33: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:50:33: #3 Pre-compute pvalue-qvalue table... WARNING @ Sun, 02 Jun 2019 20:50:33: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You may need to consider one of the other alternative d(s): 2,39 WARNING @ Sun, 02 Jun 2019 20:50:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:50:33: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:50:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 20:50:58: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:50:59: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:50:59: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05_peaks.xls INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.05_summits.bed INFO @ Sun, 02 Jun 2019 20:51:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (582 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10_peaks.xls INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.10_summits.bed INFO @ Sun, 02 Jun 2019 20:51:11: Done! INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20_peaks.xls INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:51:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495074/SRX495074.20_summits.bed INFO @ Sun, 02 Jun 2019 20:51:11: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (285 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (99 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。