Job ID = 2590182 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198602.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:52 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139780 (6.55%) aligned 0 times 37485177 (78.17%) aligned exactly 1 time 7325658 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:11:52 Overall time: 00:11:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188917 / 44810835 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 20:22:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:22:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:22:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:22:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:22:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:22:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:22:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:22:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:22:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:22:22: 1000000 INFO @ Mon, 12 Aug 2019 20:22:24: 1000000 INFO @ Mon, 12 Aug 2019 20:22:25: 1000000 INFO @ Mon, 12 Aug 2019 20:22:30: 2000000 INFO @ Mon, 12 Aug 2019 20:22:33: 2000000 INFO @ Mon, 12 Aug 2019 20:22:33: 2000000 INFO @ Mon, 12 Aug 2019 20:22:37: 3000000 INFO @ Mon, 12 Aug 2019 20:22:42: 3000000 INFO @ Mon, 12 Aug 2019 20:22:42: 3000000 INFO @ Mon, 12 Aug 2019 20:22:45: 4000000 INFO @ Mon, 12 Aug 2019 20:22:51: 4000000 INFO @ Mon, 12 Aug 2019 20:22:51: 4000000 INFO @ Mon, 12 Aug 2019 20:22:53: 5000000 INFO @ Mon, 12 Aug 2019 20:22:59: 5000000 INFO @ Mon, 12 Aug 2019 20:23:00: 5000000 INFO @ Mon, 12 Aug 2019 20:23:01: 6000000 INFO @ Mon, 12 Aug 2019 20:23:08: 6000000 INFO @ Mon, 12 Aug 2019 20:23:09: 7000000 INFO @ Mon, 12 Aug 2019 20:23:09: 6000000 INFO @ Mon, 12 Aug 2019 20:23:16: 8000000 INFO @ Mon, 12 Aug 2019 20:23:16: 7000000 INFO @ Mon, 12 Aug 2019 20:23:18: 7000000 INFO @ Mon, 12 Aug 2019 20:23:24: 9000000 INFO @ Mon, 12 Aug 2019 20:23:25: 8000000 INFO @ Mon, 12 Aug 2019 20:23:27: 8000000 INFO @ Mon, 12 Aug 2019 20:23:32: 10000000 INFO @ Mon, 12 Aug 2019 20:23:34: 9000000 INFO @ Mon, 12 Aug 2019 20:23:36: 9000000 INFO @ Mon, 12 Aug 2019 20:23:40: 11000000 INFO @ Mon, 12 Aug 2019 20:23:42: 10000000 INFO @ Mon, 12 Aug 2019 20:23:44: 10000000 INFO @ Mon, 12 Aug 2019 20:23:47: 12000000 INFO @ Mon, 12 Aug 2019 20:23:51: 11000000 INFO @ Mon, 12 Aug 2019 20:23:53: 11000000 INFO @ Mon, 12 Aug 2019 20:23:55: 13000000 INFO @ Mon, 12 Aug 2019 20:24:00: 12000000 INFO @ Mon, 12 Aug 2019 20:24:01: 12000000 INFO @ Mon, 12 Aug 2019 20:24:03: 14000000 INFO @ Mon, 12 Aug 2019 20:24:08: 13000000 INFO @ Mon, 12 Aug 2019 20:24:10: 13000000 INFO @ Mon, 12 Aug 2019 20:24:11: 15000000 INFO @ Mon, 12 Aug 2019 20:24:17: 14000000 INFO @ Mon, 12 Aug 2019 20:24:18: 14000000 INFO @ Mon, 12 Aug 2019 20:24:18: 16000000 INFO @ Mon, 12 Aug 2019 20:24:25: 15000000 INFO @ Mon, 12 Aug 2019 20:24:26: 17000000 INFO @ Mon, 12 Aug 2019 20:24:27: 15000000 INFO @ Mon, 12 Aug 2019 20:24:34: 18000000 INFO @ Mon, 12 Aug 2019 20:24:34: 16000000 INFO @ Mon, 12 Aug 2019 20:24:35: 16000000 INFO @ Mon, 12 Aug 2019 20:24:42: 19000000 INFO @ Mon, 12 Aug 2019 20:24:43: 17000000 INFO @ Mon, 12 Aug 2019 20:24:44: 17000000 INFO @ Mon, 12 Aug 2019 20:24:49: 20000000 INFO @ Mon, 12 Aug 2019 20:24:51: 18000000 INFO @ Mon, 12 Aug 2019 20:24:52: 18000000 INFO @ Mon, 12 Aug 2019 20:24:57: 21000000 INFO @ Mon, 12 Aug 2019 20:25:00: 19000000 INFO @ Mon, 12 Aug 2019 20:25:01: 19000000 INFO @ Mon, 12 Aug 2019 20:25:05: 22000000 INFO @ Mon, 12 Aug 2019 20:25:08: 20000000 INFO @ Mon, 12 Aug 2019 20:25:10: 20000000 INFO @ Mon, 12 Aug 2019 20:25:13: 23000000 INFO @ Mon, 12 Aug 2019 20:25:17: 21000000 INFO @ Mon, 12 Aug 2019 20:25:18: 21000000 INFO @ Mon, 12 Aug 2019 20:25:20: 24000000 INFO @ Mon, 12 Aug 2019 20:25:26: 22000000 INFO @ Mon, 12 Aug 2019 20:25:27: 22000000 INFO @ Mon, 12 Aug 2019 20:25:28: 25000000 INFO @ Mon, 12 Aug 2019 20:25:34: 23000000 INFO @ Mon, 12 Aug 2019 20:25:36: 23000000 INFO @ Mon, 12 Aug 2019 20:25:36: 26000000 INFO @ Mon, 12 Aug 2019 20:25:43: 24000000 INFO @ Mon, 12 Aug 2019 20:25:44: 27000000 INFO @ Mon, 12 Aug 2019 20:25:44: 24000000 INFO @ Mon, 12 Aug 2019 20:25:51: 28000000 INFO @ Mon, 12 Aug 2019 20:25:52: 25000000 INFO @ Mon, 12 Aug 2019 20:25:53: 25000000 INFO @ Mon, 12 Aug 2019 20:25:59: 29000000 INFO @ Mon, 12 Aug 2019 20:26:00: 26000000 INFO @ Mon, 12 Aug 2019 20:26:02: 26000000 INFO @ Mon, 12 Aug 2019 20:26:07: 30000000 INFO @ Mon, 12 Aug 2019 20:26:09: 27000000 INFO @ Mon, 12 Aug 2019 20:26:10: 27000000 INFO @ Mon, 12 Aug 2019 20:26:14: 31000000 INFO @ Mon, 12 Aug 2019 20:26:17: 28000000 INFO @ Mon, 12 Aug 2019 20:26:19: 28000000 INFO @ Mon, 12 Aug 2019 20:26:22: 32000000 INFO @ Mon, 12 Aug 2019 20:26:26: 29000000 INFO @ Mon, 12 Aug 2019 20:26:27: 29000000 INFO @ Mon, 12 Aug 2019 20:26:30: 33000000 INFO @ Mon, 12 Aug 2019 20:26:34: 30000000 INFO @ Mon, 12 Aug 2019 20:26:36: 30000000 INFO @ Mon, 12 Aug 2019 20:26:38: 34000000 INFO @ Mon, 12 Aug 2019 20:26:43: 31000000 INFO @ Mon, 12 Aug 2019 20:26:44: 31000000 INFO @ Mon, 12 Aug 2019 20:26:45: 35000000 INFO @ Mon, 12 Aug 2019 20:26:51: 32000000 INFO @ Mon, 12 Aug 2019 20:26:53: 32000000 INFO @ Mon, 12 Aug 2019 20:26:54: 36000000 INFO @ Mon, 12 Aug 2019 20:26:59: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:26:59: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:26:59: #1 total tags in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:26:59: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:26:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:27:00: #1 tags after filtering in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:27:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:27:00: #1 finished! INFO @ Mon, 12 Aug 2019 20:27:00: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:27:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:27:00: 33000000 INFO @ Mon, 12 Aug 2019 20:27:01: 33000000 INFO @ Mon, 12 Aug 2019 20:27:03: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:27:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:27:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:27:08: 34000000 INFO @ Mon, 12 Aug 2019 20:27:10: 34000000 INFO @ Mon, 12 Aug 2019 20:27:17: 35000000 INFO @ Mon, 12 Aug 2019 20:27:18: 35000000 INFO @ Mon, 12 Aug 2019 20:27:26: 36000000 INFO @ Mon, 12 Aug 2019 20:27:27: 36000000 INFO @ Mon, 12 Aug 2019 20:27:32: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:27:32: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:27:32: #1 total tags in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:27:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:27:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:27:32: #1 tags after filtering in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:27:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:27:32: #1 finished! INFO @ Mon, 12 Aug 2019 20:27:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:27:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:27:33: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:27:33: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:27:33: #1 total tags in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:27:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:27:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:27:34: #1 tags after filtering in treatment: 36621918 INFO @ Mon, 12 Aug 2019 20:27:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:27:34: #1 finished! INFO @ Mon, 12 Aug 2019 20:27:34: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:27:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:27:35: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:27:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:27:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:27:37: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:27:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:27:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495070/SRX495070.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。