Job ID = 6497465 SRX = SRX494966 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T22:34:49 prefetch.2.10.7: 1) Downloading 'SRR1198498'... 2020-06-25T22:34:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T22:36:48 prefetch.2.10.7: HTTPS download succeed 2020-06-25T22:36:49 prefetch.2.10.7: 'SRR1198498' is valid 2020-06-25T22:36:49 prefetch.2.10.7: 1) 'SRR1198498' was downloaded successfully Read 19040559 spots for SRR1198498/SRR1198498.sra Written 19040559 spots for SRR1198498/SRR1198498.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:28 19040559 reads; of these: 19040559 (100.00%) were unpaired; of these: 793936 (4.17%) aligned 0 times 15280074 (80.25%) aligned exactly 1 time 2966549 (15.58%) aligned >1 times 95.83% overall alignment rate Time searching: 00:04:29 Overall time: 00:04:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10943465 / 18246623 = 0.5998 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:46:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:46:25: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:46:25: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:46:30: 1000000 INFO @ Fri, 26 Jun 2020 07:46:35: 2000000 INFO @ Fri, 26 Jun 2020 07:46:40: 3000000 INFO @ Fri, 26 Jun 2020 07:46:45: 4000000 INFO @ Fri, 26 Jun 2020 07:46:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:46:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:46:55: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:46:55: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:46:55: 6000000 INFO @ Fri, 26 Jun 2020 07:47:00: 1000000 INFO @ Fri, 26 Jun 2020 07:47:01: 7000000 INFO @ Fri, 26 Jun 2020 07:47:02: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:47:02: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:47:02: #1 total tags in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:47:02: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:47:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:47:02: #1 tags after filtering in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:47:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:47:02: #1 finished! INFO @ Fri, 26 Jun 2020 07:47:02: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:47:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:47:03: #2 number of paired peaks: 518 WARNING @ Fri, 26 Jun 2020 07:47:03: Fewer paired peaks (518) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 518 pairs to build model! INFO @ Fri, 26 Jun 2020 07:47:03: start model_add_line... INFO @ Fri, 26 Jun 2020 07:47:03: start X-correlation... INFO @ Fri, 26 Jun 2020 07:47:03: end of X-cor INFO @ Fri, 26 Jun 2020 07:47:03: #2 finished! INFO @ Fri, 26 Jun 2020 07:47:03: #2 predicted fragment length is 50 bps INFO @ Fri, 26 Jun 2020 07:47:03: #2 alternative fragment length(s) may be 3,50,558 bps INFO @ Fri, 26 Jun 2020 07:47:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05_model.r WARNING @ Fri, 26 Jun 2020 07:47:03: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:47:03: #2 You may need to consider one of the other alternative d(s): 3,50,558 WARNING @ Fri, 26 Jun 2020 07:47:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:47:03: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:47:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:47:06: 2000000 INFO @ Fri, 26 Jun 2020 07:47:11: 3000000 INFO @ Fri, 26 Jun 2020 07:47:16: 4000000 INFO @ Fri, 26 Jun 2020 07:47:17: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 07:47:21: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:47:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05_peaks.xls INFO @ Fri, 26 Jun 2020 07:47:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:47:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.05_summits.bed INFO @ Fri, 26 Jun 2020 07:47:24: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (773 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:47:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:47:25: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:47:25: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:47:26: 6000000 INFO @ Fri, 26 Jun 2020 07:47:31: 1000000 INFO @ Fri, 26 Jun 2020 07:47:31: 7000000 INFO @ Fri, 26 Jun 2020 07:47:33: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:47:33: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:47:33: #1 total tags in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:47:33: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:47:33: #1 tags after filtering in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:47:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:47:33: #1 finished! INFO @ Fri, 26 Jun 2020 07:47:33: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:47:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:47:33: #2 number of paired peaks: 518 WARNING @ Fri, 26 Jun 2020 07:47:33: Fewer paired peaks (518) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 518 pairs to build model! INFO @ Fri, 26 Jun 2020 07:47:33: start model_add_line... INFO @ Fri, 26 Jun 2020 07:47:33: start X-correlation... INFO @ Fri, 26 Jun 2020 07:47:33: end of X-cor INFO @ Fri, 26 Jun 2020 07:47:33: #2 finished! INFO @ Fri, 26 Jun 2020 07:47:33: #2 predicted fragment length is 50 bps INFO @ Fri, 26 Jun 2020 07:47:33: #2 alternative fragment length(s) may be 3,50,558 bps INFO @ Fri, 26 Jun 2020 07:47:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10_model.r WARNING @ Fri, 26 Jun 2020 07:47:33: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:47:33: #2 You may need to consider one of the other alternative d(s): 3,50,558 WARNING @ Fri, 26 Jun 2020 07:47:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:47:33: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:47:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:47:36: 2000000 INFO @ Fri, 26 Jun 2020 07:47:42: 3000000 INFO @ Fri, 26 Jun 2020 07:47:47: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 07:47:48: 4000000 INFO @ Fri, 26 Jun 2020 07:47:53: 5000000 INFO @ Fri, 26 Jun 2020 07:47:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10_peaks.xls INFO @ Fri, 26 Jun 2020 07:47:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:47:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.10_summits.bed INFO @ Fri, 26 Jun 2020 07:47:55: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (564 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:47:58: 6000000 INFO @ Fri, 26 Jun 2020 07:48:03: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 07:48:05: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:48:05: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:48:05: #1 total tags in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:48:05: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:48:05: #1 tags after filtering in treatment: 7303158 INFO @ Fri, 26 Jun 2020 07:48:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:48:05: #1 finished! INFO @ Fri, 26 Jun 2020 07:48:05: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:48:06: #2 number of paired peaks: 518 WARNING @ Fri, 26 Jun 2020 07:48:06: Fewer paired peaks (518) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 518 pairs to build model! INFO @ Fri, 26 Jun 2020 07:48:06: start model_add_line... INFO @ Fri, 26 Jun 2020 07:48:06: start X-correlation... INFO @ Fri, 26 Jun 2020 07:48:06: end of X-cor INFO @ Fri, 26 Jun 2020 07:48:06: #2 finished! INFO @ Fri, 26 Jun 2020 07:48:06: #2 predicted fragment length is 50 bps INFO @ Fri, 26 Jun 2020 07:48:06: #2 alternative fragment length(s) may be 3,50,558 bps INFO @ Fri, 26 Jun 2020 07:48:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20_model.r WARNING @ Fri, 26 Jun 2020 07:48:06: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:48:06: #2 You may need to consider one of the other alternative d(s): 3,50,558 WARNING @ Fri, 26 Jun 2020 07:48:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:48:06: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:48:06: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:48:19: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 07:48:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20_peaks.xls INFO @ Fri, 26 Jun 2020 07:48:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:48:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494966/SRX494966.20_summits.bed INFO @ Fri, 26 Jun 2020 07:48:27: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (245 records, 4 fields): 2 millis CompletedMACS2peakCalling