Job ID = 6497434 SRX = SRX494932 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T22:08:01 prefetch.2.10.7: 1) Downloading 'SRR1198464'... 2020-06-25T22:08:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T22:13:10 prefetch.2.10.7: HTTPS download succeed 2020-06-25T22:13:10 prefetch.2.10.7: 1) 'SRR1198464' was downloaded successfully Read 47950615 spots for SRR1198464/SRR1198464.sra Written 47950615 spots for SRR1198464/SRR1198464.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139789 (6.55%) aligned 0 times 37484858 (78.17%) aligned exactly 1 time 7325968 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8187895 / 44810826 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:39:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:39:44: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:39:44: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:39:51: 1000000 INFO @ Fri, 26 Jun 2020 07:39:58: 2000000 INFO @ Fri, 26 Jun 2020 07:40:04: 3000000 INFO @ Fri, 26 Jun 2020 07:40:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:40:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:40:14: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:40:14: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:40:16: 5000000 INFO @ Fri, 26 Jun 2020 07:40:23: 1000000 INFO @ Fri, 26 Jun 2020 07:40:23: 6000000 INFO @ Fri, 26 Jun 2020 07:40:29: 7000000 INFO @ Fri, 26 Jun 2020 07:40:31: 2000000 INFO @ Fri, 26 Jun 2020 07:40:36: 8000000 INFO @ Fri, 26 Jun 2020 07:40:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:40:43: 9000000 INFO @ Fri, 26 Jun 2020 07:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:40:44: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:40:44: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:40:47: 4000000 INFO @ Fri, 26 Jun 2020 07:40:49: 10000000 INFO @ Fri, 26 Jun 2020 07:40:53: 1000000 INFO @ Fri, 26 Jun 2020 07:40:56: 5000000 INFO @ Fri, 26 Jun 2020 07:40:56: 11000000 INFO @ Fri, 26 Jun 2020 07:41:01: 2000000 INFO @ Fri, 26 Jun 2020 07:41:03: 12000000 INFO @ Fri, 26 Jun 2020 07:41:04: 6000000 INFO @ Fri, 26 Jun 2020 07:41:09: 13000000 INFO @ Fri, 26 Jun 2020 07:41:09: 3000000 INFO @ Fri, 26 Jun 2020 07:41:13: 7000000 INFO @ Fri, 26 Jun 2020 07:41:16: 14000000 INFO @ Fri, 26 Jun 2020 07:41:18: 4000000 INFO @ Fri, 26 Jun 2020 07:41:21: 8000000 INFO @ Fri, 26 Jun 2020 07:41:22: 15000000 INFO @ Fri, 26 Jun 2020 07:41:26: 5000000 INFO @ Fri, 26 Jun 2020 07:41:29: 16000000 INFO @ Fri, 26 Jun 2020 07:41:30: 9000000 INFO @ Fri, 26 Jun 2020 07:41:35: 6000000 INFO @ Fri, 26 Jun 2020 07:41:36: 17000000 INFO @ Fri, 26 Jun 2020 07:41:38: 10000000 INFO @ Fri, 26 Jun 2020 07:41:42: 18000000 INFO @ Fri, 26 Jun 2020 07:41:43: 7000000 INFO @ Fri, 26 Jun 2020 07:41:46: 11000000 INFO @ Fri, 26 Jun 2020 07:41:49: 19000000 INFO @ Fri, 26 Jun 2020 07:41:51: 8000000 INFO @ Fri, 26 Jun 2020 07:41:55: 12000000 INFO @ Fri, 26 Jun 2020 07:41:56: 20000000 INFO @ Fri, 26 Jun 2020 07:42:00: 9000000 INFO @ Fri, 26 Jun 2020 07:42:03: 13000000 INFO @ Fri, 26 Jun 2020 07:42:03: 21000000 INFO @ Fri, 26 Jun 2020 07:42:08: 10000000 INFO @ Fri, 26 Jun 2020 07:42:10: 22000000 INFO @ Fri, 26 Jun 2020 07:42:11: 14000000 INFO @ Fri, 26 Jun 2020 07:42:16: 11000000 INFO @ Fri, 26 Jun 2020 07:42:17: 23000000 INFO @ Fri, 26 Jun 2020 07:42:20: 15000000 INFO @ Fri, 26 Jun 2020 07:42:24: 24000000 INFO @ Fri, 26 Jun 2020 07:42:25: 12000000 INFO @ Fri, 26 Jun 2020 07:42:28: 16000000 INFO @ Fri, 26 Jun 2020 07:42:30: 25000000 INFO @ Fri, 26 Jun 2020 07:42:33: 13000000 INFO @ Fri, 26 Jun 2020 07:42:37: 17000000 INFO @ Fri, 26 Jun 2020 07:42:37: 26000000 INFO @ Fri, 26 Jun 2020 07:42:41: 14000000 INFO @ Fri, 26 Jun 2020 07:42:44: 27000000 INFO @ Fri, 26 Jun 2020 07:42:45: 18000000 INFO @ Fri, 26 Jun 2020 07:42:50: 15000000 INFO @ Fri, 26 Jun 2020 07:42:51: 28000000 INFO @ Fri, 26 Jun 2020 07:42:53: 19000000 INFO @ Fri, 26 Jun 2020 07:42:57: 29000000 INFO @ Fri, 26 Jun 2020 07:42:58: 16000000 INFO @ Fri, 26 Jun 2020 07:43:01: 20000000 INFO @ Fri, 26 Jun 2020 07:43:04: 30000000 INFO @ Fri, 26 Jun 2020 07:43:06: 17000000 INFO @ Fri, 26 Jun 2020 07:43:10: 21000000 INFO @ Fri, 26 Jun 2020 07:43:11: 31000000 INFO @ Fri, 26 Jun 2020 07:43:15: 18000000 INFO @ Fri, 26 Jun 2020 07:43:17: 32000000 INFO @ Fri, 26 Jun 2020 07:43:18: 22000000 INFO @ Fri, 26 Jun 2020 07:43:23: 19000000 INFO @ Fri, 26 Jun 2020 07:43:24: 33000000 INFO @ Fri, 26 Jun 2020 07:43:27: 23000000 INFO @ Fri, 26 Jun 2020 07:43:31: 34000000 INFO @ Fri, 26 Jun 2020 07:43:31: 20000000 INFO @ Fri, 26 Jun 2020 07:43:35: 24000000 INFO @ Fri, 26 Jun 2020 07:43:37: 35000000 INFO @ Fri, 26 Jun 2020 07:43:40: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 07:43:43: 25000000 INFO @ Fri, 26 Jun 2020 07:43:44: 36000000 INFO @ Fri, 26 Jun 2020 07:43:48: 22000000 INFO @ Fri, 26 Jun 2020 07:43:48: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:43:48: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:43:48: #1 total tags in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:43:48: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:43:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:43:49: #1 tags after filtering in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:43:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:43:49: #1 finished! INFO @ Fri, 26 Jun 2020 07:43:49: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:43:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:43:51: 26000000 INFO @ Fri, 26 Jun 2020 07:43:51: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:43:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:43:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:43:56: 23000000 INFO @ Fri, 26 Jun 2020 07:43:59: 27000000 INFO @ Fri, 26 Jun 2020 07:44:04: 24000000 INFO @ Fri, 26 Jun 2020 07:44:07: 28000000 INFO @ Fri, 26 Jun 2020 07:44:11: 25000000 INFO @ Fri, 26 Jun 2020 07:44:15: 29000000 INFO @ Fri, 26 Jun 2020 07:44:19: 26000000 INFO @ Fri, 26 Jun 2020 07:44:22: 30000000 INFO @ Fri, 26 Jun 2020 07:44:27: 27000000 INFO @ Fri, 26 Jun 2020 07:44:30: 31000000 INFO @ Fri, 26 Jun 2020 07:44:35: 28000000 INFO @ Fri, 26 Jun 2020 07:44:38: 32000000 INFO @ Fri, 26 Jun 2020 07:44:43: 29000000 INFO @ Fri, 26 Jun 2020 07:44:46: 33000000 INFO @ Fri, 26 Jun 2020 07:44:51: 30000000 INFO @ Fri, 26 Jun 2020 07:44:54: 34000000 INFO @ Fri, 26 Jun 2020 07:44:59: 31000000 INFO @ Fri, 26 Jun 2020 07:45:02: 35000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 07:45:07: 32000000 INFO @ Fri, 26 Jun 2020 07:45:10: 36000000 INFO @ Fri, 26 Jun 2020 07:45:15: 33000000 INFO @ Fri, 26 Jun 2020 07:45:15: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:45:15: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:45:15: #1 total tags in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:45:15: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:45:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:45:16: #1 tags after filtering in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:45:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:45:16: #1 finished! INFO @ Fri, 26 Jun 2020 07:45:16: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:45:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:45:18: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:45:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:45:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:45:23: 34000000 INFO @ Fri, 26 Jun 2020 07:45:31: 35000000 INFO @ Fri, 26 Jun 2020 07:45:39: 36000000 INFO @ Fri, 26 Jun 2020 07:45:44: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 07:45:44: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 07:45:44: #1 total tags in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:45:44: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:45:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:45:44: #1 tags after filtering in treatment: 36622931 INFO @ Fri, 26 Jun 2020 07:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 07:45:44: #1 finished! INFO @ Fri, 26 Jun 2020 07:45:44: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:45:47: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 07:45:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 07:45:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494932/SRX494932.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling