Job ID = 4303046 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198443.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:52 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139826 (6.55%) aligned 0 times 37484890 (78.17%) aligned exactly 1 time 7325899 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:52 Overall time: 00:10:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188282 / 44810789 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:01:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:01:39: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:01:39: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:01:45: 1000000 INFO @ Thu, 12 Dec 2019 01:01:51: 2000000 INFO @ Thu, 12 Dec 2019 01:01:57: 3000000 INFO @ Thu, 12 Dec 2019 01:02:04: 4000000 INFO @ Thu, 12 Dec 2019 01:02:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:02:09: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:02:09: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:02:10: 5000000 INFO @ Thu, 12 Dec 2019 01:02:15: 1000000 INFO @ Thu, 12 Dec 2019 01:02:16: 6000000 INFO @ Thu, 12 Dec 2019 01:02:21: 2000000 INFO @ Thu, 12 Dec 2019 01:02:22: 7000000 INFO @ Thu, 12 Dec 2019 01:02:27: 3000000 INFO @ Thu, 12 Dec 2019 01:02:28: 8000000 INFO @ Thu, 12 Dec 2019 01:02:33: 4000000 INFO @ Thu, 12 Dec 2019 01:02:35: 9000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:02:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:02:39: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:02:39: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:02:39: 5000000 INFO @ Thu, 12 Dec 2019 01:02:41: 10000000 INFO @ Thu, 12 Dec 2019 01:02:45: 6000000 INFO @ Thu, 12 Dec 2019 01:02:46: 1000000 INFO @ Thu, 12 Dec 2019 01:02:48: 11000000 INFO @ Thu, 12 Dec 2019 01:02:51: 7000000 INFO @ Thu, 12 Dec 2019 01:02:53: 2000000 INFO @ Thu, 12 Dec 2019 01:02:55: 12000000 INFO @ Thu, 12 Dec 2019 01:02:57: 8000000 INFO @ Thu, 12 Dec 2019 01:02:59: 3000000 INFO @ Thu, 12 Dec 2019 01:03:02: 13000000 INFO @ Thu, 12 Dec 2019 01:03:03: 9000000 INFO @ Thu, 12 Dec 2019 01:03:06: 4000000 INFO @ Thu, 12 Dec 2019 01:03:09: 10000000 INFO @ Thu, 12 Dec 2019 01:03:09: 14000000 INFO @ Thu, 12 Dec 2019 01:03:13: 5000000 INFO @ Thu, 12 Dec 2019 01:03:15: 11000000 INFO @ Thu, 12 Dec 2019 01:03:17: 15000000 INFO @ Thu, 12 Dec 2019 01:03:20: 6000000 INFO @ Thu, 12 Dec 2019 01:03:21: 12000000 INFO @ Thu, 12 Dec 2019 01:03:24: 16000000 INFO @ Thu, 12 Dec 2019 01:03:26: 7000000 INFO @ Thu, 12 Dec 2019 01:03:27: 13000000 INFO @ Thu, 12 Dec 2019 01:03:31: 17000000 INFO @ Thu, 12 Dec 2019 01:03:33: 14000000 INFO @ Thu, 12 Dec 2019 01:03:33: 8000000 INFO @ Thu, 12 Dec 2019 01:03:38: 18000000 INFO @ Thu, 12 Dec 2019 01:03:39: 15000000 INFO @ Thu, 12 Dec 2019 01:03:40: 9000000 INFO @ Thu, 12 Dec 2019 01:03:45: 19000000 INFO @ Thu, 12 Dec 2019 01:03:45: 16000000 INFO @ Thu, 12 Dec 2019 01:03:47: 10000000 INFO @ Thu, 12 Dec 2019 01:03:51: 17000000 INFO @ Thu, 12 Dec 2019 01:03:51: 20000000 INFO @ Thu, 12 Dec 2019 01:03:54: 11000000 INFO @ Thu, 12 Dec 2019 01:03:57: 18000000 INFO @ Thu, 12 Dec 2019 01:03:58: 21000000 INFO @ Thu, 12 Dec 2019 01:04:01: 12000000 INFO @ Thu, 12 Dec 2019 01:04:03: 19000000 INFO @ Thu, 12 Dec 2019 01:04:05: 22000000 INFO @ Thu, 12 Dec 2019 01:04:08: 13000000 INFO @ Thu, 12 Dec 2019 01:04:09: 20000000 INFO @ Thu, 12 Dec 2019 01:04:12: 23000000 INFO @ Thu, 12 Dec 2019 01:04:14: 14000000 INFO @ Thu, 12 Dec 2019 01:04:15: 21000000 INFO @ Thu, 12 Dec 2019 01:04:19: 24000000 INFO @ Thu, 12 Dec 2019 01:04:21: 22000000 INFO @ Thu, 12 Dec 2019 01:04:21: 15000000 INFO @ Thu, 12 Dec 2019 01:04:26: 25000000 INFO @ Thu, 12 Dec 2019 01:04:27: 23000000 INFO @ Thu, 12 Dec 2019 01:04:28: 16000000 INFO @ Thu, 12 Dec 2019 01:04:33: 26000000 INFO @ Thu, 12 Dec 2019 01:04:33: 24000000 INFO @ Thu, 12 Dec 2019 01:04:35: 17000000 INFO @ Thu, 12 Dec 2019 01:04:39: 25000000 INFO @ Thu, 12 Dec 2019 01:04:40: 27000000 INFO @ Thu, 12 Dec 2019 01:04:42: 18000000 INFO @ Thu, 12 Dec 2019 01:04:45: 26000000 INFO @ Thu, 12 Dec 2019 01:04:46: 28000000 INFO @ Thu, 12 Dec 2019 01:04:49: 19000000 INFO @ Thu, 12 Dec 2019 01:04:51: 27000000 INFO @ Thu, 12 Dec 2019 01:04:53: 29000000 INFO @ Thu, 12 Dec 2019 01:04:55: 20000000 INFO @ Thu, 12 Dec 2019 01:04:57: 28000000 INFO @ Thu, 12 Dec 2019 01:05:00: 30000000 INFO @ Thu, 12 Dec 2019 01:05:02: 21000000 INFO @ Thu, 12 Dec 2019 01:05:03: 29000000 INFO @ Thu, 12 Dec 2019 01:05:07: 31000000 INFO @ Thu, 12 Dec 2019 01:05:09: 22000000 INFO @ Thu, 12 Dec 2019 01:05:09: 30000000 INFO @ Thu, 12 Dec 2019 01:05:14: 32000000 INFO @ Thu, 12 Dec 2019 01:05:15: 31000000 INFO @ Thu, 12 Dec 2019 01:05:15: 23000000 INFO @ Thu, 12 Dec 2019 01:05:20: 33000000 INFO @ Thu, 12 Dec 2019 01:05:20: 32000000 INFO @ Thu, 12 Dec 2019 01:05:22: 24000000 INFO @ Thu, 12 Dec 2019 01:05:26: 33000000 INFO @ Thu, 12 Dec 2019 01:05:27: 34000000 INFO @ Thu, 12 Dec 2019 01:05:29: 25000000 INFO @ Thu, 12 Dec 2019 01:05:32: 34000000 INFO @ Thu, 12 Dec 2019 01:05:34: 35000000 INFO @ Thu, 12 Dec 2019 01:05:35: 26000000 INFO @ Thu, 12 Dec 2019 01:05:38: 35000000 INFO @ Thu, 12 Dec 2019 01:05:41: 36000000 INFO @ Thu, 12 Dec 2019 01:05:42: 27000000 INFO @ Thu, 12 Dec 2019 01:05:44: 36000000 INFO @ Thu, 12 Dec 2019 01:05:45: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:05:45: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:05:45: #1 total tags in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:05:45: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:05:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:05:46: #1 tags after filtering in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:05:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:05:46: #1 finished! INFO @ Thu, 12 Dec 2019 01:05:46: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:05:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:05:48: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:05:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:05:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Thu, 12 Dec 2019 01:05:48: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:05:48: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:05:48: #1 total tags in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:05:48: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:05:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:05:48: 28000000 INFO @ Thu, 12 Dec 2019 01:05:49: #1 tags after filtering in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:05:49: #1 finished! INFO @ Thu, 12 Dec 2019 01:05:49: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:05:51: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:05:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:05:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:05:54: 29000000 INFO @ Thu, 12 Dec 2019 01:06:00: 30000000 INFO @ Thu, 12 Dec 2019 01:06:06: 31000000 INFO @ Thu, 12 Dec 2019 01:06:12: 32000000 INFO @ Thu, 12 Dec 2019 01:06:17: 33000000 INFO @ Thu, 12 Dec 2019 01:06:23: 34000000 INFO @ Thu, 12 Dec 2019 01:06:29: 35000000 INFO @ Thu, 12 Dec 2019 01:06:35: 36000000 INFO @ Thu, 12 Dec 2019 01:06:39: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:06:39: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:06:39: #1 total tags in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:06:39: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:06:39: #1 tags after filtering in treatment: 36622507 INFO @ Thu, 12 Dec 2019 01:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:06:39: #1 finished! INFO @ Thu, 12 Dec 2019 01:06:39: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:06:42: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:06:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:06:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494911/SRX494911.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。