Job ID = 2590035 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198411.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:07 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139798 (6.55%) aligned 0 times 37485105 (78.17%) aligned exactly 1 time 7325712 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:07 Overall time: 00:12:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188515 / 44810817 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:37:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:37:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:37:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:37:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:37:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:37:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:37:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:37:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:37:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:37:50: 1000000 INFO @ Mon, 12 Aug 2019 19:37:51: 1000000 INFO @ Mon, 12 Aug 2019 19:37:52: 1000000 INFO @ Mon, 12 Aug 2019 19:37:57: 2000000 INFO @ Mon, 12 Aug 2019 19:37:58: 2000000 INFO @ Mon, 12 Aug 2019 19:38:00: 2000000 INFO @ Mon, 12 Aug 2019 19:38:05: 3000000 INFO @ Mon, 12 Aug 2019 19:38:05: 3000000 INFO @ Mon, 12 Aug 2019 19:38:07: 3000000 INFO @ Mon, 12 Aug 2019 19:38:12: 4000000 INFO @ Mon, 12 Aug 2019 19:38:12: 4000000 INFO @ Mon, 12 Aug 2019 19:38:15: 4000000 INFO @ Mon, 12 Aug 2019 19:38:19: 5000000 INFO @ Mon, 12 Aug 2019 19:38:20: 5000000 INFO @ Mon, 12 Aug 2019 19:38:23: 5000000 INFO @ Mon, 12 Aug 2019 19:38:26: 6000000 INFO @ Mon, 12 Aug 2019 19:38:27: 6000000 INFO @ Mon, 12 Aug 2019 19:38:31: 6000000 INFO @ Mon, 12 Aug 2019 19:38:33: 7000000 INFO @ Mon, 12 Aug 2019 19:38:34: 7000000 INFO @ Mon, 12 Aug 2019 19:38:38: 7000000 INFO @ Mon, 12 Aug 2019 19:38:40: 8000000 INFO @ Mon, 12 Aug 2019 19:38:41: 8000000 INFO @ Mon, 12 Aug 2019 19:38:46: 8000000 INFO @ Mon, 12 Aug 2019 19:38:47: 9000000 INFO @ Mon, 12 Aug 2019 19:38:48: 9000000 INFO @ Mon, 12 Aug 2019 19:38:53: 9000000 INFO @ Mon, 12 Aug 2019 19:38:54: 10000000 INFO @ Mon, 12 Aug 2019 19:38:55: 10000000 INFO @ Mon, 12 Aug 2019 19:39:01: 10000000 INFO @ Mon, 12 Aug 2019 19:39:01: 11000000 INFO @ Mon, 12 Aug 2019 19:39:02: 11000000 INFO @ Mon, 12 Aug 2019 19:39:08: 12000000 INFO @ Mon, 12 Aug 2019 19:39:09: 11000000 INFO @ Mon, 12 Aug 2019 19:39:09: 12000000 INFO @ Mon, 12 Aug 2019 19:39:16: 13000000 INFO @ Mon, 12 Aug 2019 19:39:16: 12000000 INFO @ Mon, 12 Aug 2019 19:39:16: 13000000 INFO @ Mon, 12 Aug 2019 19:39:22: 14000000 INFO @ Mon, 12 Aug 2019 19:39:23: 14000000 INFO @ Mon, 12 Aug 2019 19:39:24: 13000000 INFO @ Mon, 12 Aug 2019 19:39:29: 15000000 INFO @ Mon, 12 Aug 2019 19:39:30: 15000000 INFO @ Mon, 12 Aug 2019 19:39:31: 14000000 INFO @ Mon, 12 Aug 2019 19:39:36: 16000000 INFO @ Mon, 12 Aug 2019 19:39:38: 16000000 INFO @ Mon, 12 Aug 2019 19:39:39: 15000000 INFO @ Mon, 12 Aug 2019 19:39:43: 17000000 INFO @ Mon, 12 Aug 2019 19:39:45: 17000000 INFO @ Mon, 12 Aug 2019 19:39:46: 16000000 INFO @ Mon, 12 Aug 2019 19:39:51: 18000000 INFO @ Mon, 12 Aug 2019 19:39:52: 18000000 INFO @ Mon, 12 Aug 2019 19:39:54: 17000000 INFO @ Mon, 12 Aug 2019 19:39:58: 19000000 INFO @ Mon, 12 Aug 2019 19:39:59: 19000000 INFO @ Mon, 12 Aug 2019 19:40:02: 18000000 INFO @ Mon, 12 Aug 2019 19:40:05: 20000000 INFO @ Mon, 12 Aug 2019 19:40:07: 20000000 INFO @ Mon, 12 Aug 2019 19:40:10: 19000000 INFO @ Mon, 12 Aug 2019 19:40:12: 21000000 INFO @ Mon, 12 Aug 2019 19:40:14: 21000000 INFO @ Mon, 12 Aug 2019 19:40:18: 20000000 INFO @ Mon, 12 Aug 2019 19:40:19: 22000000 INFO @ Mon, 12 Aug 2019 19:40:21: 22000000 INFO @ Mon, 12 Aug 2019 19:40:25: 21000000 INFO @ Mon, 12 Aug 2019 19:40:26: 23000000 INFO @ Mon, 12 Aug 2019 19:40:29: 23000000 INFO @ Mon, 12 Aug 2019 19:40:33: 22000000 INFO @ Mon, 12 Aug 2019 19:40:34: 24000000 INFO @ Mon, 12 Aug 2019 19:40:36: 24000000 INFO @ Mon, 12 Aug 2019 19:40:41: 25000000 INFO @ Mon, 12 Aug 2019 19:40:41: 23000000 INFO @ Mon, 12 Aug 2019 19:40:43: 25000000 INFO @ Mon, 12 Aug 2019 19:40:48: 26000000 INFO @ Mon, 12 Aug 2019 19:40:49: 24000000 INFO @ Mon, 12 Aug 2019 19:40:50: 26000000 INFO @ Mon, 12 Aug 2019 19:40:55: 27000000 INFO @ Mon, 12 Aug 2019 19:40:56: 25000000 INFO @ Mon, 12 Aug 2019 19:40:57: 27000000 INFO @ Mon, 12 Aug 2019 19:41:02: 28000000 INFO @ Mon, 12 Aug 2019 19:41:04: 26000000 INFO @ Mon, 12 Aug 2019 19:41:05: 28000000 INFO @ Mon, 12 Aug 2019 19:41:09: 29000000 INFO @ Mon, 12 Aug 2019 19:41:12: 27000000 INFO @ Mon, 12 Aug 2019 19:41:12: 29000000 INFO @ Mon, 12 Aug 2019 19:41:16: 30000000 INFO @ Mon, 12 Aug 2019 19:41:19: 30000000 INFO @ Mon, 12 Aug 2019 19:41:19: 28000000 INFO @ Mon, 12 Aug 2019 19:41:23: 31000000 INFO @ Mon, 12 Aug 2019 19:41:26: 31000000 INFO @ Mon, 12 Aug 2019 19:41:27: 29000000 INFO @ Mon, 12 Aug 2019 19:41:30: 32000000 INFO @ Mon, 12 Aug 2019 19:41:33: 32000000 INFO @ Mon, 12 Aug 2019 19:41:35: 30000000 INFO @ Mon, 12 Aug 2019 19:41:37: 33000000 INFO @ Mon, 12 Aug 2019 19:41:41: 33000000 INFO @ Mon, 12 Aug 2019 19:41:43: 31000000 INFO @ Mon, 12 Aug 2019 19:41:45: 34000000 INFO @ Mon, 12 Aug 2019 19:41:49: 34000000 INFO @ Mon, 12 Aug 2019 19:41:50: 32000000 INFO @ Mon, 12 Aug 2019 19:41:52: 35000000 INFO @ Mon, 12 Aug 2019 19:41:57: 35000000 INFO @ Mon, 12 Aug 2019 19:41:58: 33000000 INFO @ Mon, 12 Aug 2019 19:41:59: 36000000 INFO @ Mon, 12 Aug 2019 19:42:04: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:42:04: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:42:04: #1 total tags in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:04: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:42:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:42:05: 36000000 INFO @ Mon, 12 Aug 2019 19:42:05: #1 tags after filtering in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:42:05: #1 finished! INFO @ Mon, 12 Aug 2019 19:42:05: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:42:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:42:06: 34000000 INFO @ Mon, 12 Aug 2019 19:42:08: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:42:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:42:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:42:09: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:42:09: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:42:09: #1 total tags in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:09: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:42:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:42:10: #1 tags after filtering in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:42:10: #1 finished! INFO @ Mon, 12 Aug 2019 19:42:10: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:42:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:42:13: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:42:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:42:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:42:13: 35000000 INFO @ Mon, 12 Aug 2019 19:42:21: 36000000 INFO @ Mon, 12 Aug 2019 19:42:26: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:42:26: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:42:26: #1 total tags in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:26: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:42:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:42:27: #1 tags after filtering in treatment: 36622302 INFO @ Mon, 12 Aug 2019 19:42:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:42:27: #1 finished! INFO @ Mon, 12 Aug 2019 19:42:27: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:42:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:42:30: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:42:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:42:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494879/SRX494879.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。