Job ID = 2590025 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:18 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139790 (6.55%) aligned 0 times 37485099 (78.17%) aligned exactly 1 time 7325726 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:13:18 Overall time: 00:13:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188374 / 44810825 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:59:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:13: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:13: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:59:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:59:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:59:21: 1000000 INFO @ Mon, 12 Aug 2019 19:59:22: 1000000 INFO @ Mon, 12 Aug 2019 19:59:22: 1000000 INFO @ Mon, 12 Aug 2019 19:59:29: 2000000 INFO @ Mon, 12 Aug 2019 19:59:29: 2000000 INFO @ Mon, 12 Aug 2019 19:59:30: 2000000 INFO @ Mon, 12 Aug 2019 19:59:36: 3000000 INFO @ Mon, 12 Aug 2019 19:59:36: 3000000 INFO @ Mon, 12 Aug 2019 19:59:38: 3000000 INFO @ Mon, 12 Aug 2019 19:59:43: 4000000 INFO @ Mon, 12 Aug 2019 19:59:44: 4000000 INFO @ Mon, 12 Aug 2019 19:59:46: 4000000 INFO @ Mon, 12 Aug 2019 19:59:50: 5000000 INFO @ Mon, 12 Aug 2019 19:59:52: 5000000 INFO @ Mon, 12 Aug 2019 19:59:55: 5000000 INFO @ Mon, 12 Aug 2019 19:59:57: 6000000 INFO @ Mon, 12 Aug 2019 19:59:59: 6000000 INFO @ Mon, 12 Aug 2019 20:00:04: 6000000 INFO @ Mon, 12 Aug 2019 20:00:04: 7000000 INFO @ Mon, 12 Aug 2019 20:00:07: 7000000 INFO @ Mon, 12 Aug 2019 20:00:11: 8000000 INFO @ Mon, 12 Aug 2019 20:00:12: 7000000 INFO @ Mon, 12 Aug 2019 20:00:15: 8000000 INFO @ Mon, 12 Aug 2019 20:00:18: 9000000 INFO @ Mon, 12 Aug 2019 20:00:21: 8000000 INFO @ Mon, 12 Aug 2019 20:00:23: 9000000 INFO @ Mon, 12 Aug 2019 20:00:25: 10000000 INFO @ Mon, 12 Aug 2019 20:00:30: 9000000 INFO @ Mon, 12 Aug 2019 20:00:30: 10000000 INFO @ Mon, 12 Aug 2019 20:00:32: 11000000 INFO @ Mon, 12 Aug 2019 20:00:38: 11000000 INFO @ Mon, 12 Aug 2019 20:00:38: 10000000 INFO @ Mon, 12 Aug 2019 20:00:39: 12000000 INFO @ Mon, 12 Aug 2019 20:00:46: 12000000 INFO @ Mon, 12 Aug 2019 20:00:46: 13000000 INFO @ Mon, 12 Aug 2019 20:00:47: 11000000 INFO @ Mon, 12 Aug 2019 20:00:53: 14000000 INFO @ Mon, 12 Aug 2019 20:00:53: 13000000 INFO @ Mon, 12 Aug 2019 20:00:56: 12000000 INFO @ Mon, 12 Aug 2019 20:01:00: 15000000 INFO @ Mon, 12 Aug 2019 20:01:01: 14000000 INFO @ Mon, 12 Aug 2019 20:01:04: 13000000 INFO @ Mon, 12 Aug 2019 20:01:07: 16000000 INFO @ Mon, 12 Aug 2019 20:01:09: 15000000 INFO @ Mon, 12 Aug 2019 20:01:13: 14000000 INFO @ Mon, 12 Aug 2019 20:01:14: 17000000 INFO @ Mon, 12 Aug 2019 20:01:16: 16000000 INFO @ Mon, 12 Aug 2019 20:01:21: 18000000 INFO @ Mon, 12 Aug 2019 20:01:22: 15000000 INFO @ Mon, 12 Aug 2019 20:01:24: 17000000 INFO @ Mon, 12 Aug 2019 20:01:28: 19000000 INFO @ Mon, 12 Aug 2019 20:01:30: 16000000 INFO @ Mon, 12 Aug 2019 20:01:32: 18000000 INFO @ Mon, 12 Aug 2019 20:01:35: 20000000 INFO @ Mon, 12 Aug 2019 20:01:39: 17000000 INFO @ Mon, 12 Aug 2019 20:01:39: 19000000 INFO @ Mon, 12 Aug 2019 20:01:42: 21000000 INFO @ Mon, 12 Aug 2019 20:01:47: 20000000 INFO @ Mon, 12 Aug 2019 20:01:47: 18000000 INFO @ Mon, 12 Aug 2019 20:01:49: 22000000 INFO @ Mon, 12 Aug 2019 20:01:55: 21000000 INFO @ Mon, 12 Aug 2019 20:01:56: 23000000 INFO @ Mon, 12 Aug 2019 20:01:56: 19000000 INFO @ Mon, 12 Aug 2019 20:02:03: 22000000 INFO @ Mon, 12 Aug 2019 20:02:03: 24000000 INFO @ Mon, 12 Aug 2019 20:02:05: 20000000 INFO @ Mon, 12 Aug 2019 20:02:10: 25000000 INFO @ Mon, 12 Aug 2019 20:02:10: 23000000 INFO @ Mon, 12 Aug 2019 20:02:13: 21000000 INFO @ Mon, 12 Aug 2019 20:02:17: 26000000 INFO @ Mon, 12 Aug 2019 20:02:18: 24000000 INFO @ Mon, 12 Aug 2019 20:02:22: 22000000 INFO @ Mon, 12 Aug 2019 20:02:24: 27000000 INFO @ Mon, 12 Aug 2019 20:02:26: 25000000 INFO @ Mon, 12 Aug 2019 20:02:30: 23000000 INFO @ Mon, 12 Aug 2019 20:02:31: 28000000 INFO @ Mon, 12 Aug 2019 20:02:33: 26000000 INFO @ Mon, 12 Aug 2019 20:02:38: 29000000 INFO @ Mon, 12 Aug 2019 20:02:39: 24000000 INFO @ Mon, 12 Aug 2019 20:02:41: 27000000 INFO @ Mon, 12 Aug 2019 20:02:44: 30000000 INFO @ Mon, 12 Aug 2019 20:02:48: 25000000 INFO @ Mon, 12 Aug 2019 20:02:48: 28000000 INFO @ Mon, 12 Aug 2019 20:02:51: 31000000 INFO @ Mon, 12 Aug 2019 20:02:56: 29000000 INFO @ Mon, 12 Aug 2019 20:02:56: 26000000 INFO @ Mon, 12 Aug 2019 20:02:58: 32000000 INFO @ Mon, 12 Aug 2019 20:03:04: 30000000 INFO @ Mon, 12 Aug 2019 20:03:05: 27000000 INFO @ Mon, 12 Aug 2019 20:03:05: 33000000 INFO @ Mon, 12 Aug 2019 20:03:11: 31000000 INFO @ Mon, 12 Aug 2019 20:03:12: 34000000 INFO @ Mon, 12 Aug 2019 20:03:13: 28000000 INFO @ Mon, 12 Aug 2019 20:03:19: 32000000 INFO @ Mon, 12 Aug 2019 20:03:19: 35000000 INFO @ Mon, 12 Aug 2019 20:03:22: 29000000 INFO @ Mon, 12 Aug 2019 20:03:26: 36000000 INFO @ Mon, 12 Aug 2019 20:03:27: 33000000 INFO @ Mon, 12 Aug 2019 20:03:31: 30000000 INFO @ Mon, 12 Aug 2019 20:03:31: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:03:31: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:03:31: #1 total tags in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:03:31: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:03:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:03:32: #1 tags after filtering in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:03:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:03:32: #1 finished! INFO @ Mon, 12 Aug 2019 20:03:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:03:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:03:34: 34000000 INFO @ Mon, 12 Aug 2019 20:03:35: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:03:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:03:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:03:39: 31000000 INFO @ Mon, 12 Aug 2019 20:03:42: 35000000 INFO @ Mon, 12 Aug 2019 20:03:48: 32000000 INFO @ Mon, 12 Aug 2019 20:03:50: 36000000 INFO @ Mon, 12 Aug 2019 20:03:55: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:03:55: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:03:55: #1 total tags in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:03:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:03:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:03:55: #1 tags after filtering in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:03:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:03:55: #1 finished! INFO @ Mon, 12 Aug 2019 20:03:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:03:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:03:56: 33000000 INFO @ Mon, 12 Aug 2019 20:03:59: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:03:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:03:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:04:05: 34000000 INFO @ Mon, 12 Aug 2019 20:04:13: 35000000 INFO @ Mon, 12 Aug 2019 20:04:22: 36000000 INFO @ Mon, 12 Aug 2019 20:04:27: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:04:27: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:04:27: #1 total tags in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:04:27: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:04:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:04:28: #1 tags after filtering in treatment: 36622451 INFO @ Mon, 12 Aug 2019 20:04:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:04:28: #1 finished! INFO @ Mon, 12 Aug 2019 20:04:28: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:04:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:04:31: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:04:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:04:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494869/SRX494869.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。