Job ID = 11240704 sra ファイルのダウンロード中... Completed: 329258K bytes transferred in 7 seconds (344473K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11009721 spots for /home/okishinya/chipatlas/results/ce10/SRX4456927/SRR7591868.sra Written 11009721 spots for /home/okishinya/chipatlas/results/ce10/SRX4456927/SRR7591868.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:16 11009721 reads; of these: 11009721 (100.00%) were unpaired; of these: 3527994 (32.04%) aligned 0 times 6397186 (58.10%) aligned exactly 1 time 1084541 (9.85%) aligned >1 times 67.96% overall alignment rate Time searching: 00:02:16 Overall time: 00:02:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1361304 / 7481727 = 0.1820 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 20:32:39: # Command line: callpeak -t SRX4456927.bam -f BAM -g ce -n SRX4456927.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4456927.05 # format = BAM # ChIP-seq file = ['SRX4456927.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:32:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:32:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:32:39: # Command line: callpeak -t SRX4456927.bam -f BAM -g ce -n SRX4456927.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4456927.20 # format = BAM # ChIP-seq file = ['SRX4456927.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:32:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:32:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:32:39: # Command line: callpeak -t SRX4456927.bam -f BAM -g ce -n SRX4456927.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4456927.10 # format = BAM # ChIP-seq file = ['SRX4456927.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:32:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:32:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:32:46: 1000000 INFO @ Sun, 07 Oct 2018 20:32:46: 1000000 INFO @ Sun, 07 Oct 2018 20:32:46: 1000000 INFO @ Sun, 07 Oct 2018 20:32:53: 2000000 INFO @ Sun, 07 Oct 2018 20:32:53: 2000000 INFO @ Sun, 07 Oct 2018 20:32:53: 2000000 INFO @ Sun, 07 Oct 2018 20:33:00: 3000000 INFO @ Sun, 07 Oct 2018 20:33:00: 3000000 INFO @ Sun, 07 Oct 2018 20:33:00: 3000000 INFO @ Sun, 07 Oct 2018 20:33:07: 4000000 INFO @ Sun, 07 Oct 2018 20:33:07: 4000000 INFO @ Sun, 07 Oct 2018 20:33:07: 4000000 INFO @ Sun, 07 Oct 2018 20:33:14: 5000000 INFO @ Sun, 07 Oct 2018 20:33:15: 5000000 INFO @ Sun, 07 Oct 2018 20:33:15: 5000000 INFO @ Sun, 07 Oct 2018 20:33:21: 6000000 INFO @ Sun, 07 Oct 2018 20:33:22: 6000000 INFO @ Sun, 07 Oct 2018 20:33:22: 6000000 INFO @ Sun, 07 Oct 2018 20:33:22: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:33:22: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:33:22: #1 total tags in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:22: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:33:22: #1 tags after filtering in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:33:22: #1 finished! INFO @ Sun, 07 Oct 2018 20:33:22: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:33:23: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:33:23: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:33:23: #1 total tags in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:23: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:33:23: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:33:23: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:33:23: #1 total tags in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:23: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:33:23: #1 tags after filtering in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:33:23: #1 finished! INFO @ Sun, 07 Oct 2018 20:33:23: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:33:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:33:23: #1 tags after filtering in treatment: 6120423 INFO @ Sun, 07 Oct 2018 20:33:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:33:23: #1 finished! INFO @ Sun, 07 Oct 2018 20:33:23: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:33:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:33:23: #2 number of paired peaks: 2742 INFO @ Sun, 07 Oct 2018 20:33:23: start model_add_line... INFO @ Sun, 07 Oct 2018 20:33:23: start X-correlation... INFO @ Sun, 07 Oct 2018 20:33:23: end of X-cor INFO @ Sun, 07 Oct 2018 20:33:23: #2 finished! INFO @ Sun, 07 Oct 2018 20:33:23: #2 predicted fragment length is 151 bps INFO @ Sun, 07 Oct 2018 20:33:23: #2 alternative fragment length(s) may be 151 bps INFO @ Sun, 07 Oct 2018 20:33:23: #2.2 Generate R script for model : SRX4456927.05_model.r INFO @ Sun, 07 Oct 2018 20:33:23: #3 Call peaks... INFO @ Sun, 07 Oct 2018 20:33:23: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 07 Oct 2018 20:33:23: #2 number of paired peaks: 2742 INFO @ Sun, 07 Oct 2018 20:33:23: start model_add_line... INFO @ Sun, 07 Oct 2018 20:33:23: #2 number of paired peaks: 2742 INFO @ Sun, 07 Oct 2018 20:33:23: start model_add_line... INFO @ Sun, 07 Oct 2018 20:33:24: start X-correlation... INFO @ Sun, 07 Oct 2018 20:33:24: end of X-cor INFO @ Sun, 07 Oct 2018 20:33:24: #2 finished! INFO @ Sun, 07 Oct 2018 20:33:24: #2 predicted fragment length is 151 bps INFO @ Sun, 07 Oct 2018 20:33:24: #2 alternative fragment length(s) may be 151 bps INFO @ Sun, 07 Oct 2018 20:33:24: #2.2 Generate R script for model : SRX4456927.10_model.r INFO @ Sun, 07 Oct 2018 20:33:24: #3 Call peaks... INFO @ Sun, 07 Oct 2018 20:33:24: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 07 Oct 2018 20:33:24: start X-correlation... INFO @ Sun, 07 Oct 2018 20:33:24: end of X-cor INFO @ Sun, 07 Oct 2018 20:33:24: #2 finished! INFO @ Sun, 07 Oct 2018 20:33:24: #2 predicted fragment length is 151 bps INFO @ Sun, 07 Oct 2018 20:33:24: #2 alternative fragment length(s) may be 151 bps INFO @ Sun, 07 Oct 2018 20:33:24: #2.2 Generate R script for model : SRX4456927.20_model.r INFO @ Sun, 07 Oct 2018 20:33:24: #3 Call peaks... INFO @ Sun, 07 Oct 2018 20:33:24: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 07 Oct 2018 20:33:40: #3 Call peaks for each chromosome... INFO @ Sun, 07 Oct 2018 20:33:42: #3 Call peaks for each chromosome... INFO @ Sun, 07 Oct 2018 20:33:43: #3 Call peaks for each chromosome... INFO @ Sun, 07 Oct 2018 20:33:49: #4 Write output xls file... SRX4456927.05_peaks.xls INFO @ Sun, 07 Oct 2018 20:33:49: #4 Write peak in narrowPeak format file... SRX4456927.05_peaks.narrowPeak INFO @ Sun, 07 Oct 2018 20:33:49: #4 Write summits bed file... SRX4456927.05_summits.bed INFO @ Sun, 07 Oct 2018 20:33:49: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (4003 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:33:50: #4 Write output xls file... SRX4456927.10_peaks.xls INFO @ Sun, 07 Oct 2018 20:33:50: #4 Write peak in narrowPeak format file... SRX4456927.10_peaks.narrowPeak INFO @ Sun, 07 Oct 2018 20:33:50: #4 Write summits bed file... SRX4456927.10_summits.bed INFO @ Sun, 07 Oct 2018 20:33:50: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1990 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:33:53: #4 Write output xls file... SRX4456927.20_peaks.xls INFO @ Sun, 07 Oct 2018 20:33:53: #4 Write peak in narrowPeak format file... SRX4456927.20_peaks.narrowPeak INFO @ Sun, 07 Oct 2018 20:33:53: #4 Write summits bed file... SRX4456927.20_summits.bed INFO @ Sun, 07 Oct 2018 20:33:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (810 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。