Job ID = 6497385 SRX = SRX4085365 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-25T21:28:34 prefetch.2.10.7: 1) Downloading 'SRR7167394'... 2020-06-25T21:28:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T21:36:36 prefetch.2.10.7: HTTPS download succeed 2020-06-25T21:36:37 prefetch.2.10.7: 1) 'SRR7167394' was downloaded successfully Read 38347298 spots for SRR7167394/SRR7167394.sra Written 38347298 spots for SRR7167394/SRR7167394.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:03 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:32 38347298 reads; of these: 38347298 (100.00%) were paired; of these: 5833676 (15.21%) aligned concordantly 0 times 27828858 (72.57%) aligned concordantly exactly 1 time 4684764 (12.22%) aligned concordantly >1 times ---- 5833676 pairs aligned concordantly 0 times; of these: 293873 (5.04%) aligned discordantly 1 time ---- 5539803 pairs aligned 0 times concordantly or discordantly; of these: 11079606 mates make up the pairs; of these: 9155763 (82.64%) aligned 0 times 1584310 (14.30%) aligned exactly 1 time 339533 (3.06%) aligned >1 times 88.06% overall alignment rate Time searching: 00:32:35 Overall time: 00:32:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2414133 / 32692485 = 0.0738 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:35:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:35:46: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:35:46: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:35:53: 1000000 INFO @ Fri, 26 Jun 2020 07:35:59: 2000000 INFO @ Fri, 26 Jun 2020 07:36:06: 3000000 INFO @ Fri, 26 Jun 2020 07:36:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:36:16: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:36:16: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:36:19: 5000000 INFO @ Fri, 26 Jun 2020 07:36:24: 1000000 INFO @ Fri, 26 Jun 2020 07:36:26: 6000000 INFO @ Fri, 26 Jun 2020 07:36:31: 2000000 INFO @ Fri, 26 Jun 2020 07:36:33: 7000000 INFO @ Fri, 26 Jun 2020 07:36:38: 3000000 INFO @ Fri, 26 Jun 2020 07:36:40: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 07:36:46: 4000000 INFO @ Fri, 26 Jun 2020 07:36:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 07:36:46: #1 read tag files... INFO @ Fri, 26 Jun 2020 07:36:46: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 07:36:47: 9000000 INFO @ Fri, 26 Jun 2020 07:36:52: 5000000 INFO @ Fri, 26 Jun 2020 07:36:54: 1000000 INFO @ Fri, 26 Jun 2020 07:36:55: 10000000 INFO @ Fri, 26 Jun 2020 07:36:59: 6000000 INFO @ Fri, 26 Jun 2020 07:37:01: 2000000 INFO @ Fri, 26 Jun 2020 07:37:02: 11000000 INFO @ Fri, 26 Jun 2020 07:37:07: 7000000 INFO @ Fri, 26 Jun 2020 07:37:08: 3000000 INFO @ Fri, 26 Jun 2020 07:37:09: 12000000 INFO @ Fri, 26 Jun 2020 07:37:14: 8000000 INFO @ Fri, 26 Jun 2020 07:37:15: 4000000 INFO @ Fri, 26 Jun 2020 07:37:17: 13000000 INFO @ Fri, 26 Jun 2020 07:37:22: 9000000 INFO @ Fri, 26 Jun 2020 07:37:23: 5000000 INFO @ Fri, 26 Jun 2020 07:37:24: 14000000 INFO @ Fri, 26 Jun 2020 07:37:29: 10000000 INFO @ Fri, 26 Jun 2020 07:37:30: 6000000 INFO @ Fri, 26 Jun 2020 07:37:31: 15000000 INFO @ Fri, 26 Jun 2020 07:37:37: 11000000 INFO @ Fri, 26 Jun 2020 07:37:37: 7000000 INFO @ Fri, 26 Jun 2020 07:37:39: 16000000 INFO @ Fri, 26 Jun 2020 07:37:44: 8000000 INFO @ Fri, 26 Jun 2020 07:37:45: 12000000 INFO @ Fri, 26 Jun 2020 07:37:46: 17000000 INFO @ Fri, 26 Jun 2020 07:37:51: 9000000 INFO @ Fri, 26 Jun 2020 07:37:53: 13000000 INFO @ Fri, 26 Jun 2020 07:37:54: 18000000 INFO @ Fri, 26 Jun 2020 07:37:59: 10000000 INFO @ Fri, 26 Jun 2020 07:38:01: 14000000 INFO @ Fri, 26 Jun 2020 07:38:02: 19000000 INFO @ Fri, 26 Jun 2020 07:38:06: 11000000 INFO @ Fri, 26 Jun 2020 07:38:08: 15000000 INFO @ Fri, 26 Jun 2020 07:38:10: 20000000 INFO @ Fri, 26 Jun 2020 07:38:13: 12000000 INFO @ Fri, 26 Jun 2020 07:38:15: 16000000 INFO @ Fri, 26 Jun 2020 07:38:17: 21000000 INFO @ Fri, 26 Jun 2020 07:38:20: 13000000 INFO @ Fri, 26 Jun 2020 07:38:23: 17000000 INFO @ Fri, 26 Jun 2020 07:38:25: 22000000 INFO @ Fri, 26 Jun 2020 07:38:27: 14000000 INFO @ Fri, 26 Jun 2020 07:38:30: 18000000 INFO @ Fri, 26 Jun 2020 07:38:32: 23000000 INFO @ Fri, 26 Jun 2020 07:38:34: 15000000 INFO @ Fri, 26 Jun 2020 07:38:38: 19000000 INFO @ Fri, 26 Jun 2020 07:38:40: 24000000 INFO @ Fri, 26 Jun 2020 07:38:41: 16000000 INFO @ Fri, 26 Jun 2020 07:38:45: 20000000 INFO @ Fri, 26 Jun 2020 07:38:47: 25000000 INFO @ Fri, 26 Jun 2020 07:38:47: 17000000 INFO @ Fri, 26 Jun 2020 07:38:52: 21000000 INFO @ Fri, 26 Jun 2020 07:38:54: 26000000 INFO @ Fri, 26 Jun 2020 07:38:54: 18000000 INFO @ Fri, 26 Jun 2020 07:38:59: 22000000 INFO @ Fri, 26 Jun 2020 07:39:01: 27000000 INFO @ Fri, 26 Jun 2020 07:39:01: 19000000 INFO @ Fri, 26 Jun 2020 07:39:06: 23000000 INFO @ Fri, 26 Jun 2020 07:39:08: 28000000 INFO @ Fri, 26 Jun 2020 07:39:08: 20000000 INFO @ Fri, 26 Jun 2020 07:39:14: 24000000 INFO @ Fri, 26 Jun 2020 07:39:15: 29000000 INFO @ Fri, 26 Jun 2020 07:39:16: 21000000 INFO @ Fri, 26 Jun 2020 07:39:21: 25000000 INFO @ Fri, 26 Jun 2020 07:39:22: 30000000 INFO @ Fri, 26 Jun 2020 07:39:23: 22000000 INFO @ Fri, 26 Jun 2020 07:39:29: 26000000 INFO @ Fri, 26 Jun 2020 07:39:30: 23000000 INFO @ Fri, 26 Jun 2020 07:39:30: 31000000 INFO @ Fri, 26 Jun 2020 07:39:35: 27000000 INFO @ Fri, 26 Jun 2020 07:39:37: 24000000 INFO @ Fri, 26 Jun 2020 07:39:37: 32000000 INFO @ Fri, 26 Jun 2020 07:39:43: 28000000 INFO @ Fri, 26 Jun 2020 07:39:44: 25000000 INFO @ Fri, 26 Jun 2020 07:39:44: 33000000 INFO @ Fri, 26 Jun 2020 07:39:50: 29000000 INFO @ Fri, 26 Jun 2020 07:39:51: 26000000 INFO @ Fri, 26 Jun 2020 07:39:52: 34000000 INFO @ Fri, 26 Jun 2020 07:39:57: 30000000 INFO @ Fri, 26 Jun 2020 07:39:59: 27000000 INFO @ Fri, 26 Jun 2020 07:39:59: 35000000 INFO @ Fri, 26 Jun 2020 07:40:05: 31000000 INFO @ Fri, 26 Jun 2020 07:40:06: 28000000 INFO @ Fri, 26 Jun 2020 07:40:06: 36000000 INFO @ Fri, 26 Jun 2020 07:40:12: 32000000 INFO @ Fri, 26 Jun 2020 07:40:13: 29000000 INFO @ Fri, 26 Jun 2020 07:40:13: 37000000 INFO @ Fri, 26 Jun 2020 07:40:19: 33000000 INFO @ Fri, 26 Jun 2020 07:40:19: 30000000 INFO @ Fri, 26 Jun 2020 07:40:21: 38000000 INFO @ Fri, 26 Jun 2020 07:40:26: 31000000 INFO @ Fri, 26 Jun 2020 07:40:27: 34000000 INFO @ Fri, 26 Jun 2020 07:40:28: 39000000 INFO @ Fri, 26 Jun 2020 07:40:33: 32000000 INFO @ Fri, 26 Jun 2020 07:40:34: 35000000 INFO @ Fri, 26 Jun 2020 07:40:35: 40000000 INFO @ Fri, 26 Jun 2020 07:40:40: 33000000 INFO @ Fri, 26 Jun 2020 07:40:41: 36000000 INFO @ Fri, 26 Jun 2020 07:40:43: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 07:40:47: 34000000 INFO @ Fri, 26 Jun 2020 07:40:49: 37000000 INFO @ Fri, 26 Jun 2020 07:40:50: 42000000 INFO @ Fri, 26 Jun 2020 07:40:54: 35000000 INFO @ Fri, 26 Jun 2020 07:40:56: 38000000 INFO @ Fri, 26 Jun 2020 07:40:58: 43000000 INFO @ Fri, 26 Jun 2020 07:41:01: 36000000 INFO @ Fri, 26 Jun 2020 07:41:04: 39000000 INFO @ Fri, 26 Jun 2020 07:41:05: 44000000 INFO @ Fri, 26 Jun 2020 07:41:08: 37000000 INFO @ Fri, 26 Jun 2020 07:41:11: 40000000 INFO @ Fri, 26 Jun 2020 07:41:13: 45000000 INFO @ Fri, 26 Jun 2020 07:41:15: 38000000 INFO @ Fri, 26 Jun 2020 07:41:19: 41000000 INFO @ Fri, 26 Jun 2020 07:41:20: 46000000 INFO @ Fri, 26 Jun 2020 07:41:22: 39000000 INFO @ Fri, 26 Jun 2020 07:41:26: 42000000 INFO @ Fri, 26 Jun 2020 07:41:28: 47000000 INFO @ Fri, 26 Jun 2020 07:41:29: 40000000 INFO @ Fri, 26 Jun 2020 07:41:33: 43000000 INFO @ Fri, 26 Jun 2020 07:41:35: 48000000 INFO @ Fri, 26 Jun 2020 07:41:36: 41000000 INFO @ Fri, 26 Jun 2020 07:41:41: 44000000 INFO @ Fri, 26 Jun 2020 07:41:42: 42000000 INFO @ Fri, 26 Jun 2020 07:41:43: 49000000 INFO @ Fri, 26 Jun 2020 07:41:48: 45000000 INFO @ Fri, 26 Jun 2020 07:41:49: 43000000 INFO @ Fri, 26 Jun 2020 07:41:51: 50000000 INFO @ Fri, 26 Jun 2020 07:41:56: 46000000 INFO @ Fri, 26 Jun 2020 07:41:56: 44000000 INFO @ Fri, 26 Jun 2020 07:41:58: 51000000 INFO @ Fri, 26 Jun 2020 07:42:03: 45000000 INFO @ Fri, 26 Jun 2020 07:42:03: 47000000 INFO @ Fri, 26 Jun 2020 07:42:06: 52000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 07:42:10: 46000000 INFO @ Fri, 26 Jun 2020 07:42:10: 48000000 INFO @ Fri, 26 Jun 2020 07:42:13: 53000000 INFO @ Fri, 26 Jun 2020 07:42:16: 47000000 INFO @ Fri, 26 Jun 2020 07:42:17: 49000000 INFO @ Fri, 26 Jun 2020 07:42:20: 54000000 INFO @ Fri, 26 Jun 2020 07:42:23: 48000000 INFO @ Fri, 26 Jun 2020 07:42:25: 50000000 INFO @ Fri, 26 Jun 2020 07:42:27: 55000000 INFO @ Fri, 26 Jun 2020 07:42:30: 49000000 INFO @ Fri, 26 Jun 2020 07:42:32: 51000000 INFO @ Fri, 26 Jun 2020 07:42:35: 56000000 INFO @ Fri, 26 Jun 2020 07:42:36: 50000000 INFO @ Fri, 26 Jun 2020 07:42:39: 52000000 INFO @ Fri, 26 Jun 2020 07:42:42: 57000000 INFO @ Fri, 26 Jun 2020 07:42:43: 51000000 INFO @ Fri, 26 Jun 2020 07:42:47: 53000000 INFO @ Fri, 26 Jun 2020 07:42:49: 58000000 INFO @ Fri, 26 Jun 2020 07:42:50: 52000000 INFO @ Fri, 26 Jun 2020 07:42:54: 54000000 INFO @ Fri, 26 Jun 2020 07:42:57: 53000000 INFO @ Fri, 26 Jun 2020 07:42:57: 59000000 INFO @ Fri, 26 Jun 2020 07:43:01: 55000000 INFO @ Fri, 26 Jun 2020 07:43:03: 54000000 INFO @ Fri, 26 Jun 2020 07:43:04: 60000000 INFO @ Fri, 26 Jun 2020 07:43:08: 56000000 INFO @ Fri, 26 Jun 2020 07:43:10: 55000000 INFO @ Fri, 26 Jun 2020 07:43:12: 61000000 INFO @ Fri, 26 Jun 2020 07:43:16: 57000000 INFO @ Fri, 26 Jun 2020 07:43:17: 56000000 INFO @ Fri, 26 Jun 2020 07:43:19: 62000000 INFO @ Fri, 26 Jun 2020 07:43:23: 58000000 INFO @ Fri, 26 Jun 2020 07:43:24: 57000000 INFO @ Fri, 26 Jun 2020 07:43:25: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 07:43:25: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 07:43:25: #1 total tags in treatment: 30110757 INFO @ Fri, 26 Jun 2020 07:43:25: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:43:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:43:25: #1 tags after filtering in treatment: 26257100 INFO @ Fri, 26 Jun 2020 07:43:25: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 07:43:25: #1 finished! INFO @ Fri, 26 Jun 2020 07:43:25: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:43:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:43:27: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 07:43:27: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 07:43:27: start model_add_line... INFO @ Fri, 26 Jun 2020 07:43:27: start X-correlation... INFO @ Fri, 26 Jun 2020 07:43:27: end of X-cor INFO @ Fri, 26 Jun 2020 07:43:27: #2 finished! INFO @ Fri, 26 Jun 2020 07:43:27: #2 predicted fragment length is 55 bps INFO @ Fri, 26 Jun 2020 07:43:27: #2 alternative fragment length(s) may be 2,55,598 bps INFO @ Fri, 26 Jun 2020 07:43:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05_model.r WARNING @ Fri, 26 Jun 2020 07:43:27: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:43:27: #2 You may need to consider one of the other alternative d(s): 2,55,598 WARNING @ Fri, 26 Jun 2020 07:43:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:43:27: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:43:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:43:31: 59000000 INFO @ Fri, 26 Jun 2020 07:43:32: 58000000 INFO @ Fri, 26 Jun 2020 07:43:38: 60000000 INFO @ Fri, 26 Jun 2020 07:43:38: 59000000 INFO @ Fri, 26 Jun 2020 07:43:45: 60000000 INFO @ Fri, 26 Jun 2020 07:43:46: 61000000 INFO @ Fri, 26 Jun 2020 07:43:53: 61000000 INFO @ Fri, 26 Jun 2020 07:43:54: 62000000 INFO @ Fri, 26 Jun 2020 07:43:59: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 07:43:59: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 07:43:59: #1 total tags in treatment: 30110757 INFO @ Fri, 26 Jun 2020 07:43:59: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:43:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:44:00: 62000000 INFO @ Fri, 26 Jun 2020 07:44:00: #1 tags after filtering in treatment: 26257100 INFO @ Fri, 26 Jun 2020 07:44:00: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 07:44:00: #1 finished! INFO @ Fri, 26 Jun 2020 07:44:00: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:44:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:44:02: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 07:44:02: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 07:44:02: start model_add_line... INFO @ Fri, 26 Jun 2020 07:44:02: start X-correlation... INFO @ Fri, 26 Jun 2020 07:44:02: end of X-cor INFO @ Fri, 26 Jun 2020 07:44:02: #2 finished! INFO @ Fri, 26 Jun 2020 07:44:02: #2 predicted fragment length is 55 bps INFO @ Fri, 26 Jun 2020 07:44:02: #2 alternative fragment length(s) may be 2,55,598 bps INFO @ Fri, 26 Jun 2020 07:44:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10_model.r WARNING @ Fri, 26 Jun 2020 07:44:02: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:44:02: #2 You may need to consider one of the other alternative d(s): 2,55,598 WARNING @ Fri, 26 Jun 2020 07:44:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:44:02: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:44:02: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:44:04: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 07:44:04: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 07:44:04: #1 total tags in treatment: 30110757 INFO @ Fri, 26 Jun 2020 07:44:04: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 07:44:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 07:44:05: #1 tags after filtering in treatment: 26257100 INFO @ Fri, 26 Jun 2020 07:44:05: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 07:44:05: #1 finished! INFO @ Fri, 26 Jun 2020 07:44:05: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 07:44:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 07:44:07: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 07:44:07: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 07:44:07: start model_add_line... INFO @ Fri, 26 Jun 2020 07:44:07: start X-correlation... INFO @ Fri, 26 Jun 2020 07:44:07: end of X-cor INFO @ Fri, 26 Jun 2020 07:44:07: #2 finished! INFO @ Fri, 26 Jun 2020 07:44:07: #2 predicted fragment length is 55 bps INFO @ Fri, 26 Jun 2020 07:44:07: #2 alternative fragment length(s) may be 2,55,598 bps INFO @ Fri, 26 Jun 2020 07:44:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20_model.r WARNING @ Fri, 26 Jun 2020 07:44:07: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 07:44:07: #2 You may need to consider one of the other alternative d(s): 2,55,598 WARNING @ Fri, 26 Jun 2020 07:44:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 07:44:07: #3 Call peaks... INFO @ Fri, 26 Jun 2020 07:44:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 07:44:08: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 07:44:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05_peaks.xls INFO @ Fri, 26 Jun 2020 07:44:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:44:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.05_summits.bed INFO @ Fri, 26 Jun 2020 07:44:28: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (742 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:44:43: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 07:44:49: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 07:45:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10_peaks.xls INFO @ Fri, 26 Jun 2020 07:45:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:45:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.10_summits.bed INFO @ Fri, 26 Jun 2020 07:45:03: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (359 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 07:45:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20_peaks.xls INFO @ Fri, 26 Jun 2020 07:45:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 07:45:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4085365/SRX4085365.20_summits.bed INFO @ Fri, 26 Jun 2020 07:45:09: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (117 records, 4 fields): 2 millis CompletedMACS2peakCalling