Job ID = 12264786 SRX = SRX4082392 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 34391511 spots for SRR7164217/SRR7164217.sra Written 34391511 spots for SRR7164217/SRR7164217.sra Read 27391877 spots for SRR7164218/SRR7164218.sra Written 27391877 spots for SRR7164218/SRR7164218.sra Read 39188610 spots for SRR7164219/SRR7164219.sra Written 39188610 spots for SRR7164219/SRR7164219.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265573 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:05:30 100971998 reads; of these: 100971998 (100.00%) were paired; of these: 5800536 (5.74%) aligned concordantly 0 times 89607340 (88.74%) aligned concordantly exactly 1 time 5564122 (5.51%) aligned concordantly >1 times ---- 5800536 pairs aligned concordantly 0 times; of these: 954559 (16.46%) aligned discordantly 1 time ---- 4845977 pairs aligned 0 times concordantly or discordantly; of these: 9691954 mates make up the pairs; of these: 8466337 (87.35%) aligned 0 times 960654 (9.91%) aligned exactly 1 time 264963 (2.73%) aligned >1 times 95.81% overall alignment rate Time searching: 01:05:30 Overall time: 01:05:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 80 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 66953993 / 94379193 = 0.7094 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:45:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:45:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:45:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:45:30: 1000000 INFO @ Sat, 03 Apr 2021 07:45:35: 2000000 INFO @ Sat, 03 Apr 2021 07:45:40: 3000000 INFO @ Sat, 03 Apr 2021 07:45:45: 4000000 INFO @ Sat, 03 Apr 2021 07:45:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:45:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:45:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:45:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:45:55: 6000000 INFO @ Sat, 03 Apr 2021 07:46:01: 1000000 INFO @ Sat, 03 Apr 2021 07:46:01: 7000000 INFO @ Sat, 03 Apr 2021 07:46:07: 8000000 INFO @ Sat, 03 Apr 2021 07:46:07: 2000000 INFO @ Sat, 03 Apr 2021 07:46:12: 9000000 INFO @ Sat, 03 Apr 2021 07:46:13: 3000000 INFO @ Sat, 03 Apr 2021 07:46:18: 10000000 INFO @ Sat, 03 Apr 2021 07:46:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:46:23: 11000000 INFO @ Sat, 03 Apr 2021 07:46:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:46:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:46:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:46:26: 5000000 INFO @ Sat, 03 Apr 2021 07:46:29: 12000000 INFO @ Sat, 03 Apr 2021 07:46:31: 1000000 INFO @ Sat, 03 Apr 2021 07:46:32: 6000000 INFO @ Sat, 03 Apr 2021 07:46:34: 13000000 INFO @ Sat, 03 Apr 2021 07:46:36: 2000000 INFO @ Sat, 03 Apr 2021 07:46:38: 7000000 INFO @ Sat, 03 Apr 2021 07:46:41: 14000000 INFO @ Sat, 03 Apr 2021 07:46:42: 3000000 INFO @ Sat, 03 Apr 2021 07:46:44: 8000000 INFO @ Sat, 03 Apr 2021 07:46:47: 15000000 INFO @ Sat, 03 Apr 2021 07:46:49: 4000000 INFO @ Sat, 03 Apr 2021 07:46:50: 9000000 INFO @ Sat, 03 Apr 2021 07:46:52: 16000000 INFO @ Sat, 03 Apr 2021 07:46:54: 5000000 INFO @ Sat, 03 Apr 2021 07:46:56: 10000000 INFO @ Sat, 03 Apr 2021 07:46:58: 17000000 INFO @ Sat, 03 Apr 2021 07:47:00: 6000000 INFO @ Sat, 03 Apr 2021 07:47:02: 11000000 INFO @ Sat, 03 Apr 2021 07:47:04: 18000000 INFO @ Sat, 03 Apr 2021 07:47:06: 7000000 INFO @ Sat, 03 Apr 2021 07:47:09: 12000000 INFO @ Sat, 03 Apr 2021 07:47:10: 19000000 INFO @ Sat, 03 Apr 2021 07:47:12: 8000000 INFO @ Sat, 03 Apr 2021 07:47:15: 13000000 INFO @ Sat, 03 Apr 2021 07:47:15: 20000000 INFO @ Sat, 03 Apr 2021 07:47:17: 9000000 INFO @ Sat, 03 Apr 2021 07:47:21: 21000000 INFO @ Sat, 03 Apr 2021 07:47:22: 14000000 INFO @ Sat, 03 Apr 2021 07:47:23: 10000000 INFO @ Sat, 03 Apr 2021 07:47:27: 22000000 INFO @ Sat, 03 Apr 2021 07:47:28: 15000000 INFO @ Sat, 03 Apr 2021 07:47:28: 11000000 INFO @ Sat, 03 Apr 2021 07:47:32: 23000000 INFO @ Sat, 03 Apr 2021 07:47:34: 16000000 INFO @ Sat, 03 Apr 2021 07:47:34: 12000000 INFO @ Sat, 03 Apr 2021 07:47:39: 24000000 INFO @ Sat, 03 Apr 2021 07:47:40: 17000000 INFO @ Sat, 03 Apr 2021 07:47:41: 13000000 INFO @ Sat, 03 Apr 2021 07:47:45: 25000000 INFO @ Sat, 03 Apr 2021 07:47:46: 18000000 INFO @ Sat, 03 Apr 2021 07:47:46: 14000000 INFO @ Sat, 03 Apr 2021 07:47:50: 26000000 INFO @ Sat, 03 Apr 2021 07:47:51: 19000000 INFO @ Sat, 03 Apr 2021 07:47:52: 15000000 INFO @ Sat, 03 Apr 2021 07:47:57: 27000000 INFO @ Sat, 03 Apr 2021 07:47:58: 16000000 INFO @ Sat, 03 Apr 2021 07:47:58: 20000000 INFO @ Sat, 03 Apr 2021 07:48:02: 28000000 INFO @ Sat, 03 Apr 2021 07:48:04: 17000000 INFO @ Sat, 03 Apr 2021 07:48:04: 21000000 INFO @ Sat, 03 Apr 2021 07:48:08: 29000000 INFO @ Sat, 03 Apr 2021 07:48:10: 18000000 INFO @ Sat, 03 Apr 2021 07:48:10: 22000000 INFO @ Sat, 03 Apr 2021 07:48:14: 30000000 INFO @ Sat, 03 Apr 2021 07:48:16: 19000000 INFO @ Sat, 03 Apr 2021 07:48:16: 23000000 INFO @ Sat, 03 Apr 2021 07:48:20: 31000000 INFO @ Sat, 03 Apr 2021 07:48:22: 20000000 INFO @ Sat, 03 Apr 2021 07:48:23: 24000000 INFO @ Sat, 03 Apr 2021 07:48:26: 32000000 INFO @ Sat, 03 Apr 2021 07:48:28: 21000000 INFO @ Sat, 03 Apr 2021 07:48:29: 25000000 INFO @ Sat, 03 Apr 2021 07:48:32: 33000000 INFO @ Sat, 03 Apr 2021 07:48:33: 22000000 INFO @ Sat, 03 Apr 2021 07:48:35: 26000000 INFO @ Sat, 03 Apr 2021 07:48:38: 34000000 INFO @ Sat, 03 Apr 2021 07:48:39: 23000000 INFO @ Sat, 03 Apr 2021 07:48:41: 27000000 INFO @ Sat, 03 Apr 2021 07:48:44: 35000000 INFO @ Sat, 03 Apr 2021 07:48:45: 24000000 INFO @ Sat, 03 Apr 2021 07:48:48: 28000000 INFO @ Sat, 03 Apr 2021 07:48:49: 36000000 INFO @ Sat, 03 Apr 2021 07:48:51: 25000000 INFO @ Sat, 03 Apr 2021 07:48:54: 29000000 INFO @ Sat, 03 Apr 2021 07:48:55: 37000000 INFO @ Sat, 03 Apr 2021 07:48:56: 26000000 INFO @ Sat, 03 Apr 2021 07:49:00: 30000000 INFO @ Sat, 03 Apr 2021 07:49:00: 38000000 INFO @ Sat, 03 Apr 2021 07:49:02: 27000000 INFO @ Sat, 03 Apr 2021 07:49:06: 31000000 INFO @ Sat, 03 Apr 2021 07:49:06: 39000000 INFO @ Sat, 03 Apr 2021 07:49:07: 28000000 INFO @ Sat, 03 Apr 2021 07:49:12: 40000000 INFO @ Sat, 03 Apr 2021 07:49:12: 32000000 INFO @ Sat, 03 Apr 2021 07:49:13: 29000000 INFO @ Sat, 03 Apr 2021 07:49:18: 41000000 INFO @ Sat, 03 Apr 2021 07:49:18: 33000000 INFO @ Sat, 03 Apr 2021 07:49:19: 30000000 INFO @ Sat, 03 Apr 2021 07:49:23: 42000000 INFO @ Sat, 03 Apr 2021 07:49:24: 34000000 INFO @ Sat, 03 Apr 2021 07:49:25: 31000000 INFO @ Sat, 03 Apr 2021 07:49:29: 43000000 INFO @ Sat, 03 Apr 2021 07:49:30: 35000000 INFO @ Sat, 03 Apr 2021 07:49:31: 32000000 INFO @ Sat, 03 Apr 2021 07:49:35: 44000000 INFO @ Sat, 03 Apr 2021 07:49:36: 36000000 INFO @ Sat, 03 Apr 2021 07:49:37: 33000000 INFO @ Sat, 03 Apr 2021 07:49:41: 45000000 INFO @ Sat, 03 Apr 2021 07:49:42: 37000000 INFO @ Sat, 03 Apr 2021 07:49:43: 34000000 INFO @ Sat, 03 Apr 2021 07:49:47: 46000000 INFO @ Sat, 03 Apr 2021 07:49:48: 38000000 INFO @ Sat, 03 Apr 2021 07:49:48: 35000000 INFO @ Sat, 03 Apr 2021 07:49:53: 47000000 INFO @ Sat, 03 Apr 2021 07:49:54: 36000000 INFO @ Sat, 03 Apr 2021 07:49:55: 39000000 INFO @ Sat, 03 Apr 2021 07:49:59: 48000000 INFO @ Sat, 03 Apr 2021 07:50:01: 37000000 INFO @ Sat, 03 Apr 2021 07:50:01: 40000000 INFO @ Sat, 03 Apr 2021 07:50:04: 49000000 INFO @ Sat, 03 Apr 2021 07:50:06: 38000000 INFO @ Sat, 03 Apr 2021 07:50:07: 41000000 INFO @ Sat, 03 Apr 2021 07:50:10: 50000000 INFO @ Sat, 03 Apr 2021 07:50:12: 39000000 INFO @ Sat, 03 Apr 2021 07:50:13: 42000000 INFO @ Sat, 03 Apr 2021 07:50:15: 51000000 INFO @ Sat, 03 Apr 2021 07:50:17: 40000000 INFO @ Sat, 03 Apr 2021 07:50:19: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:50:21: 52000000 INFO @ Sat, 03 Apr 2021 07:50:23: 41000000 INFO @ Sat, 03 Apr 2021 07:50:26: 44000000 INFO @ Sat, 03 Apr 2021 07:50:27: 53000000 INFO @ Sat, 03 Apr 2021 07:50:29: 42000000 INFO @ Sat, 03 Apr 2021 07:50:32: 45000000 INFO @ Sat, 03 Apr 2021 07:50:32: 54000000 INFO @ Sat, 03 Apr 2021 07:50:34: 43000000 INFO @ Sat, 03 Apr 2021 07:50:37: 55000000 INFO @ Sat, 03 Apr 2021 07:50:38: 46000000 INFO @ Sat, 03 Apr 2021 07:50:40: 44000000 INFO @ Sat, 03 Apr 2021 07:50:42: 56000000 INFO @ Sat, 03 Apr 2021 07:50:43: 47000000 INFO @ Sat, 03 Apr 2021 07:50:45: 45000000 INFO @ Sat, 03 Apr 2021 07:50:48: 57000000 INFO @ Sat, 03 Apr 2021 07:50:50: 48000000 INFO @ Sat, 03 Apr 2021 07:50:52: 46000000 INFO @ Sat, 03 Apr 2021 07:50:54: 58000000 INFO @ Sat, 03 Apr 2021 07:50:56: 49000000 INFO @ Sat, 03 Apr 2021 07:50:57: 47000000 INFO @ Sat, 03 Apr 2021 07:50:59: 59000000 INFO @ Sat, 03 Apr 2021 07:51:01: 50000000 INFO @ Sat, 03 Apr 2021 07:51:02: #1 tag size is determined as 44 bps INFO @ Sat, 03 Apr 2021 07:51:02: #1 tag size = 44 INFO @ Sat, 03 Apr 2021 07:51:02: #1 total tags in treatment: 28511837 INFO @ Sat, 03 Apr 2021 07:51:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:51:02: 48000000 INFO @ Sat, 03 Apr 2021 07:51:03: #1 tags after filtering in treatment: 16696453 INFO @ Sat, 03 Apr 2021 07:51:03: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 03 Apr 2021 07:51:03: #1 finished! INFO @ Sat, 03 Apr 2021 07:51:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:51:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:51:04: #2 number of paired peaks: 191 WARNING @ Sat, 03 Apr 2021 07:51:04: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Sat, 03 Apr 2021 07:51:04: start model_add_line... INFO @ Sat, 03 Apr 2021 07:51:04: start X-correlation... INFO @ Sat, 03 Apr 2021 07:51:04: end of X-cor INFO @ Sat, 03 Apr 2021 07:51:04: #2 finished! INFO @ Sat, 03 Apr 2021 07:51:04: #2 predicted fragment length is 79 bps INFO @ Sat, 03 Apr 2021 07:51:04: #2 alternative fragment length(s) may be 4,79,594 bps INFO @ Sat, 03 Apr 2021 07:51:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05_model.r WARNING @ Sat, 03 Apr 2021 07:51:04: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:51:04: #2 You may need to consider one of the other alternative d(s): 4,79,594 WARNING @ Sat, 03 Apr 2021 07:51:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:51:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:51:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:51:07: 51000000 INFO @ Sat, 03 Apr 2021 07:51:08: 49000000 INFO @ Sat, 03 Apr 2021 07:51:13: 50000000 INFO @ Sat, 03 Apr 2021 07:51:14: 52000000 INFO @ Sat, 03 Apr 2021 07:51:19: 51000000 INFO @ Sat, 03 Apr 2021 07:51:19: 53000000 INFO @ Sat, 03 Apr 2021 07:51:24: 52000000 INFO @ Sat, 03 Apr 2021 07:51:25: 54000000 INFO @ Sat, 03 Apr 2021 07:51:29: 53000000 INFO @ Sat, 03 Apr 2021 07:51:30: 55000000 INFO @ Sat, 03 Apr 2021 07:51:35: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:51:35: 54000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:51:37: 56000000 INFO @ Sat, 03 Apr 2021 07:51:40: 55000000 INFO @ Sat, 03 Apr 2021 07:51:43: 57000000 INFO @ Sat, 03 Apr 2021 07:51:45: 56000000 INFO @ Sat, 03 Apr 2021 07:51:48: 58000000 INFO @ Sat, 03 Apr 2021 07:51:50: 57000000 INFO @ Sat, 03 Apr 2021 07:51:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:51:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:51:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.05_summits.bed INFO @ Sat, 03 Apr 2021 07:51:51: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10611 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:51:53: 59000000 INFO @ Sat, 03 Apr 2021 07:51:56: 58000000 INFO @ Sat, 03 Apr 2021 07:51:57: #1 tag size is determined as 44 bps INFO @ Sat, 03 Apr 2021 07:51:57: #1 tag size = 44 INFO @ Sat, 03 Apr 2021 07:51:57: #1 total tags in treatment: 28511837 INFO @ Sat, 03 Apr 2021 07:51:57: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:51:58: #1 tags after filtering in treatment: 16696453 INFO @ Sat, 03 Apr 2021 07:51:58: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 03 Apr 2021 07:51:58: #1 finished! INFO @ Sat, 03 Apr 2021 07:51:58: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:51:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:51:59: #2 number of paired peaks: 191 WARNING @ Sat, 03 Apr 2021 07:51:59: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Sat, 03 Apr 2021 07:51:59: start model_add_line... INFO @ Sat, 03 Apr 2021 07:51:59: start X-correlation... INFO @ Sat, 03 Apr 2021 07:51:59: end of X-cor INFO @ Sat, 03 Apr 2021 07:51:59: #2 finished! INFO @ Sat, 03 Apr 2021 07:51:59: #2 predicted fragment length is 79 bps INFO @ Sat, 03 Apr 2021 07:51:59: #2 alternative fragment length(s) may be 4,79,594 bps INFO @ Sat, 03 Apr 2021 07:51:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10_model.r WARNING @ Sat, 03 Apr 2021 07:51:59: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:51:59: #2 You may need to consider one of the other alternative d(s): 4,79,594 WARNING @ Sat, 03 Apr 2021 07:51:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:51:59: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:51:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:52:00: 59000000 INFO @ Sat, 03 Apr 2021 07:52:04: #1 tag size is determined as 44 bps INFO @ Sat, 03 Apr 2021 07:52:04: #1 tag size = 44 INFO @ Sat, 03 Apr 2021 07:52:04: #1 total tags in treatment: 28511837 INFO @ Sat, 03 Apr 2021 07:52:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:52:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:52:04: #1 tags after filtering in treatment: 16696453 INFO @ Sat, 03 Apr 2021 07:52:04: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 03 Apr 2021 07:52:04: #1 finished! INFO @ Sat, 03 Apr 2021 07:52:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:52:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:52:05: #2 number of paired peaks: 191 WARNING @ Sat, 03 Apr 2021 07:52:05: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Sat, 03 Apr 2021 07:52:05: start model_add_line... INFO @ Sat, 03 Apr 2021 07:52:05: start X-correlation... INFO @ Sat, 03 Apr 2021 07:52:05: end of X-cor INFO @ Sat, 03 Apr 2021 07:52:05: #2 finished! INFO @ Sat, 03 Apr 2021 07:52:05: #2 predicted fragment length is 79 bps INFO @ Sat, 03 Apr 2021 07:52:05: #2 alternative fragment length(s) may be 4,79,594 bps INFO @ Sat, 03 Apr 2021 07:52:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20_model.r WARNING @ Sat, 03 Apr 2021 07:52:05: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:52:05: #2 You may need to consider one of the other alternative d(s): 4,79,594 WARNING @ Sat, 03 Apr 2021 07:52:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:52:05: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:52:05: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:52:28: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:52:35: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:52:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:52:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:52:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.10_summits.bed INFO @ Sat, 03 Apr 2021 07:52:44: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5954 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:52:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:52:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:52:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082392/SRX4082392.20_summits.bed INFO @ Sat, 03 Apr 2021 07:52:51: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2332 records, 4 fields): 4 millis CompletedMACS2peakCalling