Job ID = 1292042 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,710,753 reads read : 19,710,753 reads written : 19,710,753 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:15 19710753 reads; of these: 19710753 (100.00%) were unpaired; of these: 2862319 (14.52%) aligned 0 times 14441400 (73.27%) aligned exactly 1 time 2407034 (12.21%) aligned >1 times 85.48% overall alignment rate Time searching: 00:06:15 Overall time: 00:06:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6701505 / 16848434 = 0.3978 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:34:10: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:34:10: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:34:10: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:34:10: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:34:10: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:34:10: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:34:18: 1000000 INFO @ Sun, 02 Jun 2019 17:34:20: 1000000 INFO @ Sun, 02 Jun 2019 17:34:20: 1000000 INFO @ Sun, 02 Jun 2019 17:34:26: 2000000 INFO @ Sun, 02 Jun 2019 17:34:30: 2000000 INFO @ Sun, 02 Jun 2019 17:34:30: 2000000 INFO @ Sun, 02 Jun 2019 17:34:34: 3000000 INFO @ Sun, 02 Jun 2019 17:34:39: 3000000 INFO @ Sun, 02 Jun 2019 17:34:39: 3000000 INFO @ Sun, 02 Jun 2019 17:34:41: 4000000 INFO @ Sun, 02 Jun 2019 17:34:49: 4000000 INFO @ Sun, 02 Jun 2019 17:34:49: 4000000 INFO @ Sun, 02 Jun 2019 17:34:49: 5000000 INFO @ Sun, 02 Jun 2019 17:34:57: 6000000 INFO @ Sun, 02 Jun 2019 17:34:58: 5000000 INFO @ Sun, 02 Jun 2019 17:34:58: 5000000 INFO @ Sun, 02 Jun 2019 17:35:05: 7000000 INFO @ Sun, 02 Jun 2019 17:35:07: 6000000 INFO @ Sun, 02 Jun 2019 17:35:07: 6000000 INFO @ Sun, 02 Jun 2019 17:35:12: 8000000 INFO @ Sun, 02 Jun 2019 17:35:16: 7000000 INFO @ Sun, 02 Jun 2019 17:35:16: 7000000 INFO @ Sun, 02 Jun 2019 17:35:20: 9000000 INFO @ Sun, 02 Jun 2019 17:35:25: 8000000 INFO @ Sun, 02 Jun 2019 17:35:26: 8000000 INFO @ Sun, 02 Jun 2019 17:35:28: 10000000 INFO @ Sun, 02 Jun 2019 17:35:29: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:35:29: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:35:29: #1 total tags in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:29: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:35:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:35:29: #1 tags after filtering in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:35:29: #1 finished! INFO @ Sun, 02 Jun 2019 17:35:29: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:35:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:35:30: #2 number of paired peaks: 95 WARNING @ Sun, 02 Jun 2019 17:35:30: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:35:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:35:35: 9000000 INFO @ Sun, 02 Jun 2019 17:35:35: 9000000 INFO @ Sun, 02 Jun 2019 17:35:44: 10000000 INFO @ Sun, 02 Jun 2019 17:35:44: 10000000 INFO @ Sun, 02 Jun 2019 17:35:45: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:35:45: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:35:45: #1 total tags in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:45: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:35:45: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:35:45: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:35:45: #1 total tags in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:45: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:35:45: #1 tags after filtering in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:35:45: #1 finished! INFO @ Sun, 02 Jun 2019 17:35:45: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:35:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:35:45: #1 tags after filtering in treatment: 10146929 INFO @ Sun, 02 Jun 2019 17:35:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:35:45: #1 finished! INFO @ Sun, 02 Jun 2019 17:35:45: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:35:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:35:46: #2 number of paired peaks: 95 WARNING @ Sun, 02 Jun 2019 17:35:46: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:35:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20_peaks.narrowPeak: No such file or directory INFO @ Sun, 02 Jun 2019 17:35:46: #2 number of paired peaks: 95 WARNING @ Sun, 02 Jun 2019 17:35:46: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:35:46: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323688/SRX323688.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。