Job ID = 1292022 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 40,921,740 reads read : 81,843,480 reads written : 40,921,740 reads 0-length : 40,921,740 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:40 40921740 reads; of these: 40921740 (100.00%) were unpaired; of these: 736332 (1.80%) aligned 0 times 33517031 (81.91%) aligned exactly 1 time 6668377 (16.30%) aligned >1 times 98.20% overall alignment rate Time searching: 00:10:40 Overall time: 00:10:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5672273 / 40185408 = 0.1412 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:39:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:39:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:39:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:39:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:39:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:39:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:39:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:39:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:39:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:39:21: 1000000 INFO @ Sun, 02 Jun 2019 17:39:21: 1000000 INFO @ Sun, 02 Jun 2019 17:39:21: 1000000 INFO @ Sun, 02 Jun 2019 17:39:28: 2000000 INFO @ Sun, 02 Jun 2019 17:39:28: 2000000 INFO @ Sun, 02 Jun 2019 17:39:29: 2000000 INFO @ Sun, 02 Jun 2019 17:39:36: 3000000 INFO @ Sun, 02 Jun 2019 17:39:36: 3000000 INFO @ Sun, 02 Jun 2019 17:39:38: 3000000 INFO @ Sun, 02 Jun 2019 17:39:43: 4000000 INFO @ Sun, 02 Jun 2019 17:39:44: 4000000 INFO @ Sun, 02 Jun 2019 17:39:46: 4000000 INFO @ Sun, 02 Jun 2019 17:39:51: 5000000 INFO @ Sun, 02 Jun 2019 17:39:51: 5000000 INFO @ Sun, 02 Jun 2019 17:39:54: 5000000 INFO @ Sun, 02 Jun 2019 17:39:59: 6000000 INFO @ Sun, 02 Jun 2019 17:39:59: 6000000 INFO @ Sun, 02 Jun 2019 17:40:02: 6000000 INFO @ Sun, 02 Jun 2019 17:40:07: 7000000 INFO @ Sun, 02 Jun 2019 17:40:07: 7000000 INFO @ Sun, 02 Jun 2019 17:40:10: 7000000 INFO @ Sun, 02 Jun 2019 17:40:14: 8000000 INFO @ Sun, 02 Jun 2019 17:40:14: 8000000 INFO @ Sun, 02 Jun 2019 17:40:18: 8000000 INFO @ Sun, 02 Jun 2019 17:40:22: 9000000 INFO @ Sun, 02 Jun 2019 17:40:22: 9000000 INFO @ Sun, 02 Jun 2019 17:40:26: 9000000 INFO @ Sun, 02 Jun 2019 17:40:29: 10000000 INFO @ Sun, 02 Jun 2019 17:40:29: 10000000 INFO @ Sun, 02 Jun 2019 17:40:34: 10000000 INFO @ Sun, 02 Jun 2019 17:40:37: 11000000 INFO @ Sun, 02 Jun 2019 17:40:37: 11000000 INFO @ Sun, 02 Jun 2019 17:40:43: 11000000 INFO @ Sun, 02 Jun 2019 17:40:44: 12000000 INFO @ Sun, 02 Jun 2019 17:40:44: 12000000 INFO @ Sun, 02 Jun 2019 17:40:51: 12000000 INFO @ Sun, 02 Jun 2019 17:40:51: 13000000 INFO @ Sun, 02 Jun 2019 17:40:52: 13000000 INFO @ Sun, 02 Jun 2019 17:40:59: 13000000 INFO @ Sun, 02 Jun 2019 17:40:59: 14000000 INFO @ Sun, 02 Jun 2019 17:40:59: 14000000 INFO @ Sun, 02 Jun 2019 17:41:06: 15000000 INFO @ Sun, 02 Jun 2019 17:41:06: 15000000 INFO @ Sun, 02 Jun 2019 17:41:07: 14000000 INFO @ Sun, 02 Jun 2019 17:41:13: 16000000 INFO @ Sun, 02 Jun 2019 17:41:14: 16000000 INFO @ Sun, 02 Jun 2019 17:41:15: 15000000 INFO @ Sun, 02 Jun 2019 17:41:20: 17000000 INFO @ Sun, 02 Jun 2019 17:41:22: 17000000 INFO @ Sun, 02 Jun 2019 17:41:22: 16000000 INFO @ Sun, 02 Jun 2019 17:41:27: 18000000 INFO @ Sun, 02 Jun 2019 17:41:29: 18000000 INFO @ Sun, 02 Jun 2019 17:41:30: 17000000 INFO @ Sun, 02 Jun 2019 17:41:34: 19000000 INFO @ Sun, 02 Jun 2019 17:41:36: 19000000 INFO @ Sun, 02 Jun 2019 17:41:38: 18000000 INFO @ Sun, 02 Jun 2019 17:41:40: 20000000 INFO @ Sun, 02 Jun 2019 17:41:43: 20000000 INFO @ Sun, 02 Jun 2019 17:41:46: 19000000 INFO @ Sun, 02 Jun 2019 17:41:47: 21000000 INFO @ Sun, 02 Jun 2019 17:41:50: 21000000 INFO @ Sun, 02 Jun 2019 17:41:53: 20000000 INFO @ Sun, 02 Jun 2019 17:41:54: 22000000 INFO @ Sun, 02 Jun 2019 17:41:57: 22000000 INFO @ Sun, 02 Jun 2019 17:42:01: 21000000 INFO @ Sun, 02 Jun 2019 17:42:01: 23000000 INFO @ Sun, 02 Jun 2019 17:42:04: 23000000 INFO @ Sun, 02 Jun 2019 17:42:08: 24000000 INFO @ Sun, 02 Jun 2019 17:42:08: 22000000 INFO @ Sun, 02 Jun 2019 17:42:11: 24000000 INFO @ Sun, 02 Jun 2019 17:42:15: 25000000 INFO @ Sun, 02 Jun 2019 17:42:16: 23000000 INFO @ Sun, 02 Jun 2019 17:42:17: 25000000 INFO @ Sun, 02 Jun 2019 17:42:22: 26000000 INFO @ Sun, 02 Jun 2019 17:42:24: 24000000 INFO @ Sun, 02 Jun 2019 17:42:24: 26000000 INFO @ Sun, 02 Jun 2019 17:42:29: 27000000 INFO @ Sun, 02 Jun 2019 17:42:31: 27000000 INFO @ Sun, 02 Jun 2019 17:42:31: 25000000 INFO @ Sun, 02 Jun 2019 17:42:36: 28000000 INFO @ Sun, 02 Jun 2019 17:42:38: 28000000 INFO @ Sun, 02 Jun 2019 17:42:39: 26000000 INFO @ Sun, 02 Jun 2019 17:42:45: 29000000 INFO @ Sun, 02 Jun 2019 17:42:45: 29000000 INFO @ Sun, 02 Jun 2019 17:42:46: 27000000 INFO @ Sun, 02 Jun 2019 17:42:52: 30000000 INFO @ Sun, 02 Jun 2019 17:42:52: 30000000 INFO @ Sun, 02 Jun 2019 17:42:54: 28000000 INFO @ Sun, 02 Jun 2019 17:42:59: 31000000 INFO @ Sun, 02 Jun 2019 17:42:59: 31000000 INFO @ Sun, 02 Jun 2019 17:43:02: 29000000 INFO @ Sun, 02 Jun 2019 17:43:06: 32000000 INFO @ Sun, 02 Jun 2019 17:43:06: 32000000 INFO @ Sun, 02 Jun 2019 17:43:09: 30000000 INFO @ Sun, 02 Jun 2019 17:43:13: 33000000 INFO @ Sun, 02 Jun 2019 17:43:13: 33000000 INFO @ Sun, 02 Jun 2019 17:43:17: 31000000 INFO @ Sun, 02 Jun 2019 17:43:20: 34000000 INFO @ Sun, 02 Jun 2019 17:43:20: 34000000 INFO @ Sun, 02 Jun 2019 17:43:24: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:43:24: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:43:24: #1 total tags in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:24: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:43:24: #1 tags after filtering in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:43:24: #1 finished! INFO @ Sun, 02 Jun 2019 17:43:24: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:43:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:43:24: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:43:24: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:43:24: #1 total tags in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:24: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:43:25: 32000000 INFO @ Sun, 02 Jun 2019 17:43:25: #1 tags after filtering in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:43:25: #1 finished! INFO @ Sun, 02 Jun 2019 17:43:25: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:43:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:43:27: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 17:43:27: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:43:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:43:28: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 17:43:28: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:43:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:43:32: 33000000 INFO @ Sun, 02 Jun 2019 17:43:40: 34000000 INFO @ Sun, 02 Jun 2019 17:43:44: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:43:44: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:43:44: #1 total tags in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:43:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:43:44: #1 tags after filtering in treatment: 34513135 INFO @ Sun, 02 Jun 2019 17:43:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:43:44: #1 finished! INFO @ Sun, 02 Jun 2019 17:43:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:43:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:43:47: #2 number of paired peaks: 39 WARNING @ Sun, 02 Jun 2019 17:43:47: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:43:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3104612/SRX3104612.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。