Job ID = 12264766 SRX = SRX3029131 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 50527041 spots for SRR5860431/SRR5860431.sra Written 50527041 spots for SRR5860431/SRR5860431.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265582 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:51:37 50527041 reads; of these: 50527041 (100.00%) were paired; of these: 19029177 (37.66%) aligned concordantly 0 times 27872138 (55.16%) aligned concordantly exactly 1 time 3625726 (7.18%) aligned concordantly >1 times ---- 19029177 pairs aligned concordantly 0 times; of these: 4776852 (25.10%) aligned discordantly 1 time ---- 14252325 pairs aligned 0 times concordantly or discordantly; of these: 28504650 mates make up the pairs; of these: 26268360 (92.15%) aligned 0 times 1046883 (3.67%) aligned exactly 1 time 1189407 (4.17%) aligned >1 times 74.01% overall alignment rate Time searching: 00:51:38 Overall time: 00:51:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 10110392 / 35906280 = 0.2816 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:48:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:48:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:48:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:48:08: 1000000 INFO @ Sat, 03 Apr 2021 07:48:15: 2000000 INFO @ Sat, 03 Apr 2021 07:48:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:48:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:48:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:48:30: 4000000 INFO @ Sat, 03 Apr 2021 07:48:38: 1000000 INFO @ Sat, 03 Apr 2021 07:48:38: 5000000 INFO @ Sat, 03 Apr 2021 07:48:45: 2000000 INFO @ Sat, 03 Apr 2021 07:48:46: 6000000 INFO @ Sat, 03 Apr 2021 07:48:53: 3000000 INFO @ Sat, 03 Apr 2021 07:48:54: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:49:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:49:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:49:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:49:01: 4000000 INFO @ Sat, 03 Apr 2021 07:49:02: 8000000 INFO @ Sat, 03 Apr 2021 07:49:09: 9000000 INFO @ Sat, 03 Apr 2021 07:49:09: 5000000 INFO @ Sat, 03 Apr 2021 07:49:10: 1000000 INFO @ Sat, 03 Apr 2021 07:49:17: 10000000 INFO @ Sat, 03 Apr 2021 07:49:17: 6000000 INFO @ Sat, 03 Apr 2021 07:49:20: 2000000 INFO @ Sat, 03 Apr 2021 07:49:25: 7000000 INFO @ Sat, 03 Apr 2021 07:49:26: 11000000 INFO @ Sat, 03 Apr 2021 07:49:30: 3000000 INFO @ Sat, 03 Apr 2021 07:49:33: 8000000 INFO @ Sat, 03 Apr 2021 07:49:34: 12000000 INFO @ Sat, 03 Apr 2021 07:49:41: 9000000 INFO @ Sat, 03 Apr 2021 07:49:41: 4000000 INFO @ Sat, 03 Apr 2021 07:49:42: 13000000 INFO @ Sat, 03 Apr 2021 07:49:49: 10000000 INFO @ Sat, 03 Apr 2021 07:49:50: 14000000 INFO @ Sat, 03 Apr 2021 07:49:52: 5000000 INFO @ Sat, 03 Apr 2021 07:49:57: 11000000 INFO @ Sat, 03 Apr 2021 07:49:58: 15000000 INFO @ Sat, 03 Apr 2021 07:50:02: 6000000 INFO @ Sat, 03 Apr 2021 07:50:05: 12000000 INFO @ Sat, 03 Apr 2021 07:50:06: 16000000 INFO @ Sat, 03 Apr 2021 07:50:12: 7000000 INFO @ Sat, 03 Apr 2021 07:50:13: 13000000 INFO @ Sat, 03 Apr 2021 07:50:14: 17000000 INFO @ Sat, 03 Apr 2021 07:50:20: 14000000 INFO @ Sat, 03 Apr 2021 07:50:22: 18000000 INFO @ Sat, 03 Apr 2021 07:50:23: 8000000 INFO @ Sat, 03 Apr 2021 07:50:28: 15000000 INFO @ Sat, 03 Apr 2021 07:50:30: 19000000 INFO @ Sat, 03 Apr 2021 07:50:33: 9000000 INFO @ Sat, 03 Apr 2021 07:50:37: 16000000 INFO @ Sat, 03 Apr 2021 07:50:38: 20000000 INFO @ Sat, 03 Apr 2021 07:50:43: 10000000 INFO @ Sat, 03 Apr 2021 07:50:45: 17000000 INFO @ Sat, 03 Apr 2021 07:50:46: 21000000 INFO @ Sat, 03 Apr 2021 07:50:53: 18000000 INFO @ Sat, 03 Apr 2021 07:50:54: 11000000 INFO @ Sat, 03 Apr 2021 07:50:54: 22000000 INFO @ Sat, 03 Apr 2021 07:51:00: 19000000 INFO @ Sat, 03 Apr 2021 07:51:02: 23000000 INFO @ Sat, 03 Apr 2021 07:51:04: 12000000 INFO @ Sat, 03 Apr 2021 07:51:08: 20000000 INFO @ Sat, 03 Apr 2021 07:51:10: 24000000 INFO @ Sat, 03 Apr 2021 07:51:14: 13000000 INFO @ Sat, 03 Apr 2021 07:51:17: 21000000 INFO @ Sat, 03 Apr 2021 07:51:18: 25000000 INFO @ Sat, 03 Apr 2021 07:51:24: 14000000 INFO @ Sat, 03 Apr 2021 07:51:25: 22000000 INFO @ Sat, 03 Apr 2021 07:51:26: 26000000 INFO @ Sat, 03 Apr 2021 07:51:33: 23000000 INFO @ Sat, 03 Apr 2021 07:51:34: 15000000 INFO @ Sat, 03 Apr 2021 07:51:34: 27000000 INFO @ Sat, 03 Apr 2021 07:51:40: 24000000 INFO @ Sat, 03 Apr 2021 07:51:42: 28000000 INFO @ Sat, 03 Apr 2021 07:51:44: 16000000 INFO @ Sat, 03 Apr 2021 07:51:48: 25000000 INFO @ Sat, 03 Apr 2021 07:51:50: 29000000 INFO @ Sat, 03 Apr 2021 07:51:53: 17000000 INFO @ Sat, 03 Apr 2021 07:51:56: 26000000 INFO @ Sat, 03 Apr 2021 07:51:58: 30000000 INFO @ Sat, 03 Apr 2021 07:52:02: 18000000 INFO @ Sat, 03 Apr 2021 07:52:04: 27000000 INFO @ Sat, 03 Apr 2021 07:52:06: 31000000 INFO @ Sat, 03 Apr 2021 07:52:12: 19000000 INFO @ Sat, 03 Apr 2021 07:52:12: 28000000 INFO @ Sat, 03 Apr 2021 07:52:14: 32000000 INFO @ Sat, 03 Apr 2021 07:52:20: 29000000 INFO @ Sat, 03 Apr 2021 07:52:21: 20000000 INFO @ Sat, 03 Apr 2021 07:52:22: 33000000 INFO @ Sat, 03 Apr 2021 07:52:28: 30000000 INFO @ Sat, 03 Apr 2021 07:52:30: 34000000 INFO @ Sat, 03 Apr 2021 07:52:31: 21000000 INFO @ Sat, 03 Apr 2021 07:52:36: 31000000 INFO @ Sat, 03 Apr 2021 07:52:38: 35000000 INFO @ Sat, 03 Apr 2021 07:52:41: 22000000 INFO @ Sat, 03 Apr 2021 07:52:44: 32000000 INFO @ Sat, 03 Apr 2021 07:52:46: 36000000 INFO @ Sat, 03 Apr 2021 07:52:51: 23000000 INFO @ Sat, 03 Apr 2021 07:52:52: 33000000 INFO @ Sat, 03 Apr 2021 07:52:54: 37000000 INFO @ Sat, 03 Apr 2021 07:53:00: 34000000 INFO @ Sat, 03 Apr 2021 07:53:01: 24000000 INFO @ Sat, 03 Apr 2021 07:53:02: 38000000 INFO @ Sat, 03 Apr 2021 07:53:08: 35000000 INFO @ Sat, 03 Apr 2021 07:53:11: 39000000 INFO @ Sat, 03 Apr 2021 07:53:11: 25000000 INFO @ Sat, 03 Apr 2021 07:53:17: 36000000 INFO @ Sat, 03 Apr 2021 07:53:19: 40000000 INFO @ Sat, 03 Apr 2021 07:53:22: 26000000 INFO @ Sat, 03 Apr 2021 07:53:25: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:53:27: 41000000 INFO @ Sat, 03 Apr 2021 07:53:32: 27000000 INFO @ Sat, 03 Apr 2021 07:53:33: 38000000 INFO @ Sat, 03 Apr 2021 07:53:35: 42000000 INFO @ Sat, 03 Apr 2021 07:53:41: 28000000 INFO @ Sat, 03 Apr 2021 07:53:41: 39000000 INFO @ Sat, 03 Apr 2021 07:53:43: 43000000 INFO @ Sat, 03 Apr 2021 07:53:49: 40000000 INFO @ Sat, 03 Apr 2021 07:53:50: 29000000 INFO @ Sat, 03 Apr 2021 07:53:52: 44000000 INFO @ Sat, 03 Apr 2021 07:53:58: 41000000 INFO @ Sat, 03 Apr 2021 07:53:59: 30000000 INFO @ Sat, 03 Apr 2021 07:54:00: 45000000 INFO @ Sat, 03 Apr 2021 07:54:05: 42000000 INFO @ Sat, 03 Apr 2021 07:54:07: 31000000 INFO @ Sat, 03 Apr 2021 07:54:07: 46000000 INFO @ Sat, 03 Apr 2021 07:54:13: 43000000 INFO @ Sat, 03 Apr 2021 07:54:15: 47000000 INFO @ Sat, 03 Apr 2021 07:54:15: 32000000 INFO @ Sat, 03 Apr 2021 07:54:21: 44000000 INFO @ Sat, 03 Apr 2021 07:54:23: 48000000 INFO @ Sat, 03 Apr 2021 07:54:23: 33000000 INFO @ Sat, 03 Apr 2021 07:54:28: 45000000 INFO @ Sat, 03 Apr 2021 07:54:30: 49000000 INFO @ Sat, 03 Apr 2021 07:54:31: 34000000 INFO @ Sat, 03 Apr 2021 07:54:36: 46000000 INFO @ Sat, 03 Apr 2021 07:54:38: 50000000 INFO @ Sat, 03 Apr 2021 07:54:39: 35000000 INFO @ Sat, 03 Apr 2021 07:54:44: 47000000 INFO @ Sat, 03 Apr 2021 07:54:46: 51000000 INFO @ Sat, 03 Apr 2021 07:54:47: 36000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:54:51: 48000000 INFO @ Sat, 03 Apr 2021 07:54:53: 52000000 INFO @ Sat, 03 Apr 2021 07:54:55: 37000000 INFO @ Sat, 03 Apr 2021 07:54:59: 49000000 INFO @ Sat, 03 Apr 2021 07:55:01: 53000000 INFO @ Sat, 03 Apr 2021 07:55:03: 38000000 INFO @ Sat, 03 Apr 2021 07:55:07: 50000000 INFO @ Sat, 03 Apr 2021 07:55:08: 54000000 INFO @ Sat, 03 Apr 2021 07:55:10: 39000000 INFO @ Sat, 03 Apr 2021 07:55:13: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:55:13: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:55:13: #1 total tags in treatment: 22727981 INFO @ Sat, 03 Apr 2021 07:55:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:13: #1 tags after filtering in treatment: 16170010 INFO @ Sat, 03 Apr 2021 07:55:13: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 07:55:13: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:55:14: #2 number of paired peaks: 176 WARNING @ Sat, 03 Apr 2021 07:55:14: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Sat, 03 Apr 2021 07:55:14: start model_add_line... INFO @ Sat, 03 Apr 2021 07:55:14: start X-correlation... INFO @ Sat, 03 Apr 2021 07:55:15: end of X-cor INFO @ Sat, 03 Apr 2021 07:55:15: #2 finished! INFO @ Sat, 03 Apr 2021 07:55:15: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 07:55:15: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 07:55:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05_model.r WARNING @ Sat, 03 Apr 2021 07:55:15: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:55:15: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 07:55:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:55:15: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:55:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:55:15: 51000000 INFO @ Sat, 03 Apr 2021 07:55:18: 40000000 INFO @ Sat, 03 Apr 2021 07:55:22: 52000000 INFO @ Sat, 03 Apr 2021 07:55:26: 41000000 INFO @ Sat, 03 Apr 2021 07:55:29: 53000000 INFO @ Sat, 03 Apr 2021 07:55:34: 42000000 INFO @ Sat, 03 Apr 2021 07:55:35: 54000000 INFO @ Sat, 03 Apr 2021 07:55:40: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:55:40: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:55:40: #1 total tags in treatment: 22727981 INFO @ Sat, 03 Apr 2021 07:55:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:40: #1 tags after filtering in treatment: 16170010 INFO @ Sat, 03 Apr 2021 07:55:40: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 07:55:40: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:40: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:55:41: #2 number of paired peaks: 176 WARNING @ Sat, 03 Apr 2021 07:55:41: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Sat, 03 Apr 2021 07:55:41: start model_add_line... INFO @ Sat, 03 Apr 2021 07:55:41: start X-correlation... INFO @ Sat, 03 Apr 2021 07:55:41: end of X-cor INFO @ Sat, 03 Apr 2021 07:55:41: #2 finished! INFO @ Sat, 03 Apr 2021 07:55:41: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 07:55:41: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 07:55:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10_model.r WARNING @ Sat, 03 Apr 2021 07:55:41: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:55:41: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 07:55:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:55:41: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:55:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:55:43: 43000000 INFO @ Sat, 03 Apr 2021 07:55:44: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:55:52: 44000000 INFO @ Sat, 03 Apr 2021 07:55:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:56:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:56:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.05_summits.bed INFO @ Sat, 03 Apr 2021 07:56:00: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6577 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:56:01: 45000000 INFO @ Sat, 03 Apr 2021 07:56:09: 46000000 INFO @ Sat, 03 Apr 2021 07:56:11: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:56:18: 47000000 INFO @ Sat, 03 Apr 2021 07:56:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:56:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:56:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.10_summits.bed INFO @ Sat, 03 Apr 2021 07:56:26: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3512 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:56:27: 48000000 INFO @ Sat, 03 Apr 2021 07:56:36: 49000000 INFO @ Sat, 03 Apr 2021 07:56:46: 50000000 INFO @ Sat, 03 Apr 2021 07:56:55: 51000000 INFO @ Sat, 03 Apr 2021 07:57:04: 52000000 INFO @ Sat, 03 Apr 2021 07:57:12: 53000000 INFO @ Sat, 03 Apr 2021 07:57:20: 54000000 INFO @ Sat, 03 Apr 2021 07:57:25: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:57:25: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:57:25: #1 total tags in treatment: 22727981 INFO @ Sat, 03 Apr 2021 07:57:25: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:57:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:57:25: #1 tags after filtering in treatment: 16170010 INFO @ Sat, 03 Apr 2021 07:57:25: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 07:57:25: #1 finished! INFO @ Sat, 03 Apr 2021 07:57:25: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:57:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:57:26: #2 number of paired peaks: 176 WARNING @ Sat, 03 Apr 2021 07:57:26: Fewer paired peaks (176) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 176 pairs to build model! INFO @ Sat, 03 Apr 2021 07:57:26: start model_add_line... INFO @ Sat, 03 Apr 2021 07:57:27: start X-correlation... INFO @ Sat, 03 Apr 2021 07:57:27: end of X-cor INFO @ Sat, 03 Apr 2021 07:57:27: #2 finished! INFO @ Sat, 03 Apr 2021 07:57:27: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 07:57:27: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 07:57:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20_model.r WARNING @ Sat, 03 Apr 2021 07:57:27: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:57:27: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 07:57:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:57:27: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:57:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:57:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:58:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:58:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:58:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029131/SRX3029131.20_summits.bed INFO @ Sat, 03 Apr 2021 07:58:11: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1392 records, 4 fields): 3 millis CompletedMACS2peakCalling