Job ID = 6626344 SRX = SRX3008772 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39732741 spots for SRR5831454/SRR5831454.sra Written 39732741 spots for SRR5831454/SRR5831454.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626595 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:09:32 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 646210 (1.63%) aligned 0 times 33452843 (84.19%) aligned exactly 1 time 5633688 (14.18%) aligned >1 times 98.37% overall alignment rate Time searching: 00:09:33 Overall time: 00:09:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6880924 / 39086531 = 0.1760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:44: 1000000 INFO @ Tue, 14 Jul 2020 07:14:49: 2000000 INFO @ Tue, 14 Jul 2020 07:14:55: 3000000 INFO @ Tue, 14 Jul 2020 07:15:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:07: 5000000 INFO @ Tue, 14 Jul 2020 07:15:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:15: 6000000 INFO @ Tue, 14 Jul 2020 07:15:15: 1000000 INFO @ Tue, 14 Jul 2020 07:15:22: 7000000 INFO @ Tue, 14 Jul 2020 07:15:23: 2000000 INFO @ Tue, 14 Jul 2020 07:15:30: 8000000 INFO @ Tue, 14 Jul 2020 07:15:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:37: 9000000 INFO @ Tue, 14 Jul 2020 07:15:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:39: 4000000 INFO @ Tue, 14 Jul 2020 07:15:44: 10000000 INFO @ Tue, 14 Jul 2020 07:15:46: 1000000 INFO @ Tue, 14 Jul 2020 07:15:48: 5000000 INFO @ Tue, 14 Jul 2020 07:15:52: 11000000 INFO @ Tue, 14 Jul 2020 07:15:54: 2000000 INFO @ Tue, 14 Jul 2020 07:15:56: 6000000 INFO @ Tue, 14 Jul 2020 07:15:59: 12000000 INFO @ Tue, 14 Jul 2020 07:16:02: 3000000 INFO @ Tue, 14 Jul 2020 07:16:04: 7000000 INFO @ Tue, 14 Jul 2020 07:16:07: 13000000 INFO @ Tue, 14 Jul 2020 07:16:10: 4000000 INFO @ Tue, 14 Jul 2020 07:16:13: 8000000 INFO @ Tue, 14 Jul 2020 07:16:14: 14000000 INFO @ Tue, 14 Jul 2020 07:16:19: 5000000 INFO @ Tue, 14 Jul 2020 07:16:21: 9000000 INFO @ Tue, 14 Jul 2020 07:16:22: 15000000 INFO @ Tue, 14 Jul 2020 07:16:27: 6000000 INFO @ Tue, 14 Jul 2020 07:16:29: 10000000 INFO @ Tue, 14 Jul 2020 07:16:29: 16000000 INFO @ Tue, 14 Jul 2020 07:16:35: 7000000 INFO @ Tue, 14 Jul 2020 07:16:37: 11000000 INFO @ Tue, 14 Jul 2020 07:16:37: 17000000 INFO @ Tue, 14 Jul 2020 07:16:43: 8000000 INFO @ Tue, 14 Jul 2020 07:16:45: 18000000 INFO @ Tue, 14 Jul 2020 07:16:45: 12000000 INFO @ Tue, 14 Jul 2020 07:16:51: 9000000 INFO @ Tue, 14 Jul 2020 07:16:53: 19000000 INFO @ Tue, 14 Jul 2020 07:16:53: 13000000 INFO @ Tue, 14 Jul 2020 07:17:00: 10000000 INFO @ Tue, 14 Jul 2020 07:17:01: 20000000 INFO @ Tue, 14 Jul 2020 07:17:01: 14000000 INFO @ Tue, 14 Jul 2020 07:17:08: 11000000 INFO @ Tue, 14 Jul 2020 07:17:09: 15000000 INFO @ Tue, 14 Jul 2020 07:17:09: 21000000 INFO @ Tue, 14 Jul 2020 07:17:16: 12000000 INFO @ Tue, 14 Jul 2020 07:17:17: 16000000 INFO @ Tue, 14 Jul 2020 07:17:17: 22000000 INFO @ Tue, 14 Jul 2020 07:17:24: 13000000 INFO @ Tue, 14 Jul 2020 07:17:25: 17000000 INFO @ Tue, 14 Jul 2020 07:17:25: 23000000 INFO @ Tue, 14 Jul 2020 07:17:33: 14000000 INFO @ Tue, 14 Jul 2020 07:17:33: 18000000 INFO @ Tue, 14 Jul 2020 07:17:33: 24000000 INFO @ Tue, 14 Jul 2020 07:17:41: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:17:41: 25000000 INFO @ Tue, 14 Jul 2020 07:17:42: 15000000 INFO @ Tue, 14 Jul 2020 07:17:48: 20000000 INFO @ Tue, 14 Jul 2020 07:17:50: 26000000 INFO @ Tue, 14 Jul 2020 07:17:50: 16000000 INFO @ Tue, 14 Jul 2020 07:17:55: 21000000 INFO @ Tue, 14 Jul 2020 07:17:58: 27000000 INFO @ Tue, 14 Jul 2020 07:17:58: 17000000 INFO @ Tue, 14 Jul 2020 07:18:02: 22000000 INFO @ Tue, 14 Jul 2020 07:18:06: 28000000 INFO @ Tue, 14 Jul 2020 07:18:06: 18000000 INFO @ Tue, 14 Jul 2020 07:18:09: 23000000 INFO @ Tue, 14 Jul 2020 07:18:14: 19000000 INFO @ Tue, 14 Jul 2020 07:18:14: 29000000 INFO @ Tue, 14 Jul 2020 07:18:17: 24000000 INFO @ Tue, 14 Jul 2020 07:18:21: 20000000 INFO @ Tue, 14 Jul 2020 07:18:22: 30000000 INFO @ Tue, 14 Jul 2020 07:18:24: 25000000 INFO @ Tue, 14 Jul 2020 07:18:28: 21000000 INFO @ Tue, 14 Jul 2020 07:18:30: 31000000 INFO @ Tue, 14 Jul 2020 07:18:31: 26000000 INFO @ Tue, 14 Jul 2020 07:18:36: 22000000 INFO @ Tue, 14 Jul 2020 07:18:38: 32000000 INFO @ Tue, 14 Jul 2020 07:18:38: 27000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:18:40: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:40: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:40: #1 total tags in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:18:40: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:40: #1 tags after filtering in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:18:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:40: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:40: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:42: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 07:18:42: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:43: 23000000 INFO @ Tue, 14 Jul 2020 07:18:46: 28000000 INFO @ Tue, 14 Jul 2020 07:18:50: 24000000 INFO @ Tue, 14 Jul 2020 07:18:53: 29000000 INFO @ Tue, 14 Jul 2020 07:18:58: 25000000 INFO @ Tue, 14 Jul 2020 07:19:00: 30000000 INFO @ Tue, 14 Jul 2020 07:19:05: 26000000 INFO @ Tue, 14 Jul 2020 07:19:07: 31000000 INFO @ Tue, 14 Jul 2020 07:19:12: 27000000 INFO @ Tue, 14 Jul 2020 07:19:14: 32000000 INFO @ Tue, 14 Jul 2020 07:19:16: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:16: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:16: #1 total tags in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:19:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:16: #1 tags after filtering in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:19:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:16: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:18: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 07:19:18: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:19:19: 28000000 INFO @ Tue, 14 Jul 2020 07:19:25: 29000000 INFO @ Tue, 14 Jul 2020 07:19:31: 30000000 INFO @ Tue, 14 Jul 2020 07:19:38: 31000000 INFO @ Tue, 14 Jul 2020 07:19:44: 32000000 INFO @ Tue, 14 Jul 2020 07:19:46: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:46: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:46: #1 total tags in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:19:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:46: #1 tags after filtering in treatment: 32205607 INFO @ Tue, 14 Jul 2020 07:19:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:46: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:49: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 07:19:49: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008772/SRX3008772.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling