Job ID = 6626339 SRX = SRX3008767 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45228743 spots for SRR5831449/SRR5831449.sra Written 45228743 spots for SRR5831449/SRR5831449.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626603 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:13 45228743 reads; of these: 45228743 (100.00%) were unpaired; of these: 542342 (1.20%) aligned 0 times 36333483 (80.33%) aligned exactly 1 time 8352918 (18.47%) aligned >1 times 98.80% overall alignment rate Time searching: 00:11:13 Overall time: 00:11:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8804674 / 44686401 = 0.1970 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:44: 1000000 INFO @ Tue, 14 Jul 2020 07:17:50: 2000000 INFO @ Tue, 14 Jul 2020 07:17:56: 3000000 INFO @ Tue, 14 Jul 2020 07:18:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:18:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:18:05: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:18:05: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:18:09: 5000000 INFO @ Tue, 14 Jul 2020 07:18:14: 1000000 INFO @ Tue, 14 Jul 2020 07:18:17: 6000000 INFO @ Tue, 14 Jul 2020 07:18:21: 2000000 INFO @ Tue, 14 Jul 2020 07:18:27: 7000000 INFO @ Tue, 14 Jul 2020 07:18:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:18:34: 8000000 INFO @ Tue, 14 Jul 2020 07:18:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:18:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:18:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:18:37: 4000000 INFO @ Tue, 14 Jul 2020 07:18:42: 9000000 INFO @ Tue, 14 Jul 2020 07:18:44: 1000000 INFO @ Tue, 14 Jul 2020 07:18:46: 5000000 INFO @ Tue, 14 Jul 2020 07:18:50: 10000000 INFO @ Tue, 14 Jul 2020 07:18:52: 2000000 INFO @ Tue, 14 Jul 2020 07:18:54: 6000000 INFO @ Tue, 14 Jul 2020 07:18:58: 11000000 INFO @ Tue, 14 Jul 2020 07:18:59: 3000000 INFO @ Tue, 14 Jul 2020 07:19:02: 7000000 INFO @ Tue, 14 Jul 2020 07:19:05: 12000000 INFO @ Tue, 14 Jul 2020 07:19:07: 4000000 INFO @ Tue, 14 Jul 2020 07:19:09: 8000000 INFO @ Tue, 14 Jul 2020 07:19:13: 13000000 INFO @ Tue, 14 Jul 2020 07:19:15: 5000000 INFO @ Tue, 14 Jul 2020 07:19:17: 9000000 INFO @ Tue, 14 Jul 2020 07:19:21: 14000000 INFO @ Tue, 14 Jul 2020 07:19:23: 6000000 INFO @ Tue, 14 Jul 2020 07:19:25: 10000000 INFO @ Tue, 14 Jul 2020 07:19:28: 15000000 INFO @ Tue, 14 Jul 2020 07:19:31: 7000000 INFO @ Tue, 14 Jul 2020 07:19:32: 11000000 INFO @ Tue, 14 Jul 2020 07:19:36: 16000000 INFO @ Tue, 14 Jul 2020 07:19:38: 8000000 INFO @ Tue, 14 Jul 2020 07:19:40: 12000000 INFO @ Tue, 14 Jul 2020 07:19:43: 17000000 INFO @ Tue, 14 Jul 2020 07:19:46: 9000000 INFO @ Tue, 14 Jul 2020 07:19:47: 13000000 INFO @ Tue, 14 Jul 2020 07:19:51: 18000000 INFO @ Tue, 14 Jul 2020 07:19:54: 10000000 INFO @ Tue, 14 Jul 2020 07:19:55: 14000000 INFO @ Tue, 14 Jul 2020 07:19:58: 19000000 INFO @ Tue, 14 Jul 2020 07:20:01: 11000000 INFO @ Tue, 14 Jul 2020 07:20:02: 15000000 INFO @ Tue, 14 Jul 2020 07:20:06: 20000000 INFO @ Tue, 14 Jul 2020 07:20:09: 12000000 INFO @ Tue, 14 Jul 2020 07:20:10: 16000000 INFO @ Tue, 14 Jul 2020 07:20:13: 21000000 INFO @ Tue, 14 Jul 2020 07:20:16: 13000000 INFO @ Tue, 14 Jul 2020 07:20:17: 17000000 INFO @ Tue, 14 Jul 2020 07:20:20: 22000000 INFO @ Tue, 14 Jul 2020 07:20:24: 14000000 INFO @ Tue, 14 Jul 2020 07:20:24: 18000000 INFO @ Tue, 14 Jul 2020 07:20:27: 23000000 INFO @ Tue, 14 Jul 2020 07:20:32: 15000000 INFO @ Tue, 14 Jul 2020 07:20:32: 19000000 INFO @ Tue, 14 Jul 2020 07:20:34: 24000000 INFO @ Tue, 14 Jul 2020 07:20:39: 20000000 INFO @ Tue, 14 Jul 2020 07:20:39: 16000000 INFO @ Tue, 14 Jul 2020 07:20:41: 25000000 INFO @ Tue, 14 Jul 2020 07:20:46: 21000000 INFO @ Tue, 14 Jul 2020 07:20:46: 17000000 INFO @ Tue, 14 Jul 2020 07:20:48: 26000000 INFO @ Tue, 14 Jul 2020 07:20:53: 22000000 INFO @ Tue, 14 Jul 2020 07:20:54: 18000000 INFO @ Tue, 14 Jul 2020 07:20:56: 27000000 INFO @ Tue, 14 Jul 2020 07:21:00: 23000000 INFO @ Tue, 14 Jul 2020 07:21:01: 19000000 INFO @ Tue, 14 Jul 2020 07:21:03: 28000000 INFO @ Tue, 14 Jul 2020 07:21:08: 24000000 INFO @ Tue, 14 Jul 2020 07:21:09: 20000000 INFO @ Tue, 14 Jul 2020 07:21:10: 29000000 INFO @ Tue, 14 Jul 2020 07:21:15: 25000000 INFO @ Tue, 14 Jul 2020 07:21:16: 21000000 INFO @ Tue, 14 Jul 2020 07:21:17: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:21:22: 26000000 INFO @ Tue, 14 Jul 2020 07:21:23: 22000000 INFO @ Tue, 14 Jul 2020 07:21:24: 31000000 INFO @ Tue, 14 Jul 2020 07:21:29: 27000000 INFO @ Tue, 14 Jul 2020 07:21:30: 23000000 INFO @ Tue, 14 Jul 2020 07:21:32: 32000000 INFO @ Tue, 14 Jul 2020 07:21:36: 28000000 INFO @ Tue, 14 Jul 2020 07:21:38: 24000000 INFO @ Tue, 14 Jul 2020 07:21:39: 33000000 INFO @ Tue, 14 Jul 2020 07:21:43: 29000000 INFO @ Tue, 14 Jul 2020 07:21:45: 25000000 INFO @ Tue, 14 Jul 2020 07:21:46: 34000000 INFO @ Tue, 14 Jul 2020 07:21:50: 30000000 INFO @ Tue, 14 Jul 2020 07:21:52: 26000000 INFO @ Tue, 14 Jul 2020 07:21:53: 35000000 INFO @ Tue, 14 Jul 2020 07:21:57: 31000000 INFO @ Tue, 14 Jul 2020 07:21:59: 27000000 INFO @ Tue, 14 Jul 2020 07:21:59: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:59: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:59: #1 total tags in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:21:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:22:00: #1 tags after filtering in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:22:00: #1 finished! INFO @ Tue, 14 Jul 2020 07:22:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:22:02: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 07:22:02: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:22:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:22:04: 32000000 INFO @ Tue, 14 Jul 2020 07:22:06: 28000000 INFO @ Tue, 14 Jul 2020 07:22:10: 33000000 INFO @ Tue, 14 Jul 2020 07:22:13: 29000000 INFO @ Tue, 14 Jul 2020 07:22:17: 34000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:22:20: 30000000 INFO @ Tue, 14 Jul 2020 07:22:24: 35000000 INFO @ Tue, 14 Jul 2020 07:22:27: 31000000 INFO @ Tue, 14 Jul 2020 07:22:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:22:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:22:30: #1 total tags in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:22:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:22:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:22:30: #1 tags after filtering in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:22:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:22:30: #1 finished! INFO @ Tue, 14 Jul 2020 07:22:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:22:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:22:32: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 07:22:32: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:22:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:22:34: 32000000 INFO @ Tue, 14 Jul 2020 07:22:40: 33000000 INFO @ Tue, 14 Jul 2020 07:22:47: 34000000 INFO @ Tue, 14 Jul 2020 07:22:54: 35000000 INFO @ Tue, 14 Jul 2020 07:23:00: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:23:00: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:23:00: #1 total tags in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:23:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:23:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:23:00: #1 tags after filtering in treatment: 35881727 INFO @ Tue, 14 Jul 2020 07:23:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:23:00: #1 finished! INFO @ Tue, 14 Jul 2020 07:23:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:23:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:23:02: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 07:23:02: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:23:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008767/SRX3008767.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling