Job ID = 6626336 SRX = SRX3008764 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45176912 spots for SRR5831446/SRR5831446.sra Written 45176912 spots for SRR5831446/SRR5831446.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626590 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:09 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2585054 (5.72%) aligned 0 times 35587052 (78.77%) aligned exactly 1 time 7004806 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7350493 / 42591858 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:34: 1000000 INFO @ Tue, 14 Jul 2020 07:14:40: 2000000 INFO @ Tue, 14 Jul 2020 07:14:45: 3000000 INFO @ Tue, 14 Jul 2020 07:14:51: 4000000 INFO @ Tue, 14 Jul 2020 07:14:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:02: 6000000 INFO @ Tue, 14 Jul 2020 07:15:06: 1000000 INFO @ Tue, 14 Jul 2020 07:15:08: 7000000 INFO @ Tue, 14 Jul 2020 07:15:13: 2000000 INFO @ Tue, 14 Jul 2020 07:15:15: 8000000 INFO @ Tue, 14 Jul 2020 07:15:19: 3000000 INFO @ Tue, 14 Jul 2020 07:15:21: 9000000 INFO @ Tue, 14 Jul 2020 07:15:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:27: 10000000 INFO @ Tue, 14 Jul 2020 07:15:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:32: 5000000 INFO @ Tue, 14 Jul 2020 07:15:34: 11000000 INFO @ Tue, 14 Jul 2020 07:15:36: 1000000 INFO @ Tue, 14 Jul 2020 07:15:39: 6000000 INFO @ Tue, 14 Jul 2020 07:15:41: 12000000 INFO @ Tue, 14 Jul 2020 07:15:44: 2000000 INFO @ Tue, 14 Jul 2020 07:15:45: 7000000 INFO @ Tue, 14 Jul 2020 07:15:48: 13000000 INFO @ Tue, 14 Jul 2020 07:15:51: 3000000 INFO @ Tue, 14 Jul 2020 07:15:52: 8000000 INFO @ Tue, 14 Jul 2020 07:15:55: 14000000 INFO @ Tue, 14 Jul 2020 07:15:58: 4000000 INFO @ Tue, 14 Jul 2020 07:15:59: 9000000 INFO @ Tue, 14 Jul 2020 07:16:02: 15000000 INFO @ Tue, 14 Jul 2020 07:16:05: 5000000 INFO @ Tue, 14 Jul 2020 07:16:06: 10000000 INFO @ Tue, 14 Jul 2020 07:16:09: 16000000 INFO @ Tue, 14 Jul 2020 07:16:12: 6000000 INFO @ Tue, 14 Jul 2020 07:16:13: 11000000 INFO @ Tue, 14 Jul 2020 07:16:15: 17000000 INFO @ Tue, 14 Jul 2020 07:16:19: 7000000 INFO @ Tue, 14 Jul 2020 07:16:20: 12000000 INFO @ Tue, 14 Jul 2020 07:16:22: 18000000 INFO @ Tue, 14 Jul 2020 07:16:26: 8000000 INFO @ Tue, 14 Jul 2020 07:16:27: 13000000 INFO @ Tue, 14 Jul 2020 07:16:29: 19000000 INFO @ Tue, 14 Jul 2020 07:16:33: 9000000 INFO @ Tue, 14 Jul 2020 07:16:33: 14000000 INFO @ Tue, 14 Jul 2020 07:16:36: 20000000 INFO @ Tue, 14 Jul 2020 07:16:40: 10000000 INFO @ Tue, 14 Jul 2020 07:16:40: 15000000 INFO @ Tue, 14 Jul 2020 07:16:42: 21000000 INFO @ Tue, 14 Jul 2020 07:16:47: 11000000 INFO @ Tue, 14 Jul 2020 07:16:47: 16000000 INFO @ Tue, 14 Jul 2020 07:16:49: 22000000 INFO @ Tue, 14 Jul 2020 07:16:54: 17000000 INFO @ Tue, 14 Jul 2020 07:16:54: 12000000 INFO @ Tue, 14 Jul 2020 07:16:56: 23000000 INFO @ Tue, 14 Jul 2020 07:17:00: 18000000 INFO @ Tue, 14 Jul 2020 07:17:01: 13000000 INFO @ Tue, 14 Jul 2020 07:17:02: 24000000 INFO @ Tue, 14 Jul 2020 07:17:07: 19000000 INFO @ Tue, 14 Jul 2020 07:17:08: 14000000 INFO @ Tue, 14 Jul 2020 07:17:08: 25000000 INFO @ Tue, 14 Jul 2020 07:17:14: 20000000 INFO @ Tue, 14 Jul 2020 07:17:15: 15000000 INFO @ Tue, 14 Jul 2020 07:17:15: 26000000 INFO @ Tue, 14 Jul 2020 07:17:21: 21000000 INFO @ Tue, 14 Jul 2020 07:17:21: 27000000 INFO @ Tue, 14 Jul 2020 07:17:22: 16000000 INFO @ Tue, 14 Jul 2020 07:17:27: 22000000 INFO @ Tue, 14 Jul 2020 07:17:28: 28000000 INFO @ Tue, 14 Jul 2020 07:17:29: 17000000 INFO @ Tue, 14 Jul 2020 07:17:34: 23000000 INFO @ Tue, 14 Jul 2020 07:17:35: 29000000 INFO @ Tue, 14 Jul 2020 07:17:37: 18000000 INFO @ Tue, 14 Jul 2020 07:17:41: 24000000 INFO @ Tue, 14 Jul 2020 07:17:41: 30000000 INFO @ Tue, 14 Jul 2020 07:17:44: 19000000 INFO @ Tue, 14 Jul 2020 07:17:48: 25000000 INFO @ Tue, 14 Jul 2020 07:17:48: 31000000 INFO @ Tue, 14 Jul 2020 07:17:52: 20000000 INFO @ Tue, 14 Jul 2020 07:17:54: 26000000 INFO @ Tue, 14 Jul 2020 07:17:55: 32000000 INFO @ Tue, 14 Jul 2020 07:18:00: 21000000 INFO @ Tue, 14 Jul 2020 07:18:01: 27000000 INFO @ Tue, 14 Jul 2020 07:18:01: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:18:07: 22000000 INFO @ Tue, 14 Jul 2020 07:18:08: 28000000 INFO @ Tue, 14 Jul 2020 07:18:08: 34000000 INFO @ Tue, 14 Jul 2020 07:18:14: 23000000 INFO @ Tue, 14 Jul 2020 07:18:15: 35000000 INFO @ Tue, 14 Jul 2020 07:18:15: 29000000 INFO @ Tue, 14 Jul 2020 07:18:16: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:16: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:16: #1 total tags in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:18:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:17: #1 tags after filtering in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:18:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:17: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:19: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 07:18:19: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:21: 30000000 INFO @ Tue, 14 Jul 2020 07:18:21: 24000000 INFO @ Tue, 14 Jul 2020 07:18:28: 31000000 INFO @ Tue, 14 Jul 2020 07:18:28: 25000000 INFO @ Tue, 14 Jul 2020 07:18:34: 32000000 INFO @ Tue, 14 Jul 2020 07:18:36: 26000000 INFO @ Tue, 14 Jul 2020 07:18:41: 33000000 INFO @ Tue, 14 Jul 2020 07:18:43: 27000000 INFO @ Tue, 14 Jul 2020 07:18:47: 34000000 INFO @ Tue, 14 Jul 2020 07:18:50: 28000000 INFO @ Tue, 14 Jul 2020 07:18:54: 35000000 INFO @ Tue, 14 Jul 2020 07:18:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:55: #1 total tags in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:18:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:56: #1 tags after filtering in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:18:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:56: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:56: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:57: 29000000 INFO @ Tue, 14 Jul 2020 07:18:58: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 07:18:58: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:19:04: 30000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:19:11: 31000000 INFO @ Tue, 14 Jul 2020 07:19:17: 32000000 INFO @ Tue, 14 Jul 2020 07:19:24: 33000000 INFO @ Tue, 14 Jul 2020 07:19:30: 34000000 INFO @ Tue, 14 Jul 2020 07:19:37: 35000000 INFO @ Tue, 14 Jul 2020 07:19:38: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:38: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:38: #1 total tags in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:19:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:39: #1 tags after filtering in treatment: 35241365 INFO @ Tue, 14 Jul 2020 07:19:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:39: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:39: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:41: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 07:19:41: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008764/SRX3008764.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling