Job ID = 11170820 sra ファイルのダウンロード中... Completed: 1424520K bytes transferred in 176 seconds (66284K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 49540971 spots for /home/okishinya/chipatlas/results/ce10/SRX2973387/SRR5786982.sra Written 49540971 spots for /home/okishinya/chipatlas/results/ce10/SRX2973387/SRR5786982.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:02 49540971 reads; of these: 49540971 (100.00%) were unpaired; of these: 4122646 (8.32%) aligned 0 times 38470080 (77.65%) aligned exactly 1 time 6948245 (14.03%) aligned >1 times 91.68% overall alignment rate Time searching: 00:19:02 Overall time: 00:19:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12189916 / 45418325 = 0.2684 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:01:20: # Command line: callpeak -t SRX2973387.bam -f BAM -g ce -n SRX2973387.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2973387.10 # format = BAM # ChIP-seq file = ['SRX2973387.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:20: # Command line: callpeak -t SRX2973387.bam -f BAM -g ce -n SRX2973387.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2973387.20 # format = BAM # ChIP-seq file = ['SRX2973387.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:20: # Command line: callpeak -t SRX2973387.bam -f BAM -g ce -n SRX2973387.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2973387.05 # format = BAM # ChIP-seq file = ['SRX2973387.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:20: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:20: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:20: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:20: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:20: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:20: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:30: 1000000 INFO @ Sat, 08 Sep 2018 12:01:30: 1000000 INFO @ Sat, 08 Sep 2018 12:01:30: 1000000 INFO @ Sat, 08 Sep 2018 12:01:39: 2000000 INFO @ Sat, 08 Sep 2018 12:01:39: 2000000 INFO @ Sat, 08 Sep 2018 12:01:39: 2000000 INFO @ Sat, 08 Sep 2018 12:01:48: 3000000 INFO @ Sat, 08 Sep 2018 12:01:48: 3000000 INFO @ Sat, 08 Sep 2018 12:01:48: 3000000 INFO @ Sat, 08 Sep 2018 12:01:57: 4000000 INFO @ Sat, 08 Sep 2018 12:01:57: 4000000 INFO @ Sat, 08 Sep 2018 12:01:57: 4000000 INFO @ Sat, 08 Sep 2018 12:02:06: 5000000 INFO @ Sat, 08 Sep 2018 12:02:06: 5000000 INFO @ Sat, 08 Sep 2018 12:02:06: 5000000 INFO @ Sat, 08 Sep 2018 12:02:15: 6000000 INFO @ Sat, 08 Sep 2018 12:02:15: 6000000 INFO @ Sat, 08 Sep 2018 12:02:15: 6000000 INFO @ Sat, 08 Sep 2018 12:02:24: 7000000 INFO @ Sat, 08 Sep 2018 12:02:24: 7000000 INFO @ Sat, 08 Sep 2018 12:02:24: 7000000 INFO @ Sat, 08 Sep 2018 12:02:33: 8000000 INFO @ Sat, 08 Sep 2018 12:02:33: 8000000 INFO @ Sat, 08 Sep 2018 12:02:33: 8000000 INFO @ Sat, 08 Sep 2018 12:02:42: 9000000 INFO @ Sat, 08 Sep 2018 12:02:42: 9000000 INFO @ Sat, 08 Sep 2018 12:02:42: 9000000 INFO @ Sat, 08 Sep 2018 12:02:51: 10000000 INFO @ Sat, 08 Sep 2018 12:02:51: 10000000 INFO @ Sat, 08 Sep 2018 12:02:51: 10000000 INFO @ Sat, 08 Sep 2018 12:03:00: 11000000 INFO @ Sat, 08 Sep 2018 12:03:00: 11000000 INFO @ Sat, 08 Sep 2018 12:03:00: 11000000 INFO @ Sat, 08 Sep 2018 12:03:09: 12000000 INFO @ Sat, 08 Sep 2018 12:03:09: 12000000 INFO @ Sat, 08 Sep 2018 12:03:09: 12000000 INFO @ Sat, 08 Sep 2018 12:03:18: 13000000 INFO @ Sat, 08 Sep 2018 12:03:18: 13000000 INFO @ Sat, 08 Sep 2018 12:03:18: 13000000 INFO @ Sat, 08 Sep 2018 12:03:27: 14000000 INFO @ Sat, 08 Sep 2018 12:03:27: 14000000 INFO @ Sat, 08 Sep 2018 12:03:27: 14000000 INFO @ Sat, 08 Sep 2018 12:03:36: 15000000 INFO @ Sat, 08 Sep 2018 12:03:36: 15000000 INFO @ Sat, 08 Sep 2018 12:03:36: 15000000 INFO @ Sat, 08 Sep 2018 12:03:45: 16000000 INFO @ Sat, 08 Sep 2018 12:03:45: 16000000 INFO @ Sat, 08 Sep 2018 12:03:45: 16000000 INFO @ Sat, 08 Sep 2018 12:03:54: 17000000 INFO @ Sat, 08 Sep 2018 12:03:54: 17000000 INFO @ Sat, 08 Sep 2018 12:03:54: 17000000 INFO @ Sat, 08 Sep 2018 12:04:03: 18000000 INFO @ Sat, 08 Sep 2018 12:04:03: 18000000 INFO @ Sat, 08 Sep 2018 12:04:03: 18000000 INFO @ Sat, 08 Sep 2018 12:04:12: 19000000 INFO @ Sat, 08 Sep 2018 12:04:12: 19000000 INFO @ Sat, 08 Sep 2018 12:04:12: 19000000 INFO @ Sat, 08 Sep 2018 12:04:21: 20000000 INFO @ Sat, 08 Sep 2018 12:04:21: 20000000 INFO @ Sat, 08 Sep 2018 12:04:21: 20000000 INFO @ Sat, 08 Sep 2018 12:04:30: 21000000 INFO @ Sat, 08 Sep 2018 12:04:30: 21000000 INFO @ Sat, 08 Sep 2018 12:04:30: 21000000 INFO @ Sat, 08 Sep 2018 12:04:39: 22000000 INFO @ Sat, 08 Sep 2018 12:04:39: 22000000 INFO @ Sat, 08 Sep 2018 12:04:39: 22000000 INFO @ Sat, 08 Sep 2018 12:04:48: 23000000 INFO @ Sat, 08 Sep 2018 12:04:48: 23000000 INFO @ Sat, 08 Sep 2018 12:04:48: 23000000 INFO @ Sat, 08 Sep 2018 12:04:57: 24000000 INFO @ Sat, 08 Sep 2018 12:04:57: 24000000 INFO @ Sat, 08 Sep 2018 12:04:57: 24000000 INFO @ Sat, 08 Sep 2018 12:05:06: 25000000 INFO @ Sat, 08 Sep 2018 12:05:06: 25000000 INFO @ Sat, 08 Sep 2018 12:05:06: 25000000 INFO @ Sat, 08 Sep 2018 12:05:15: 26000000 INFO @ Sat, 08 Sep 2018 12:05:15: 26000000 INFO @ Sat, 08 Sep 2018 12:05:15: 26000000 INFO @ Sat, 08 Sep 2018 12:05:23: 27000000 INFO @ Sat, 08 Sep 2018 12:05:23: 27000000 INFO @ Sat, 08 Sep 2018 12:05:23: 27000000 INFO @ Sat, 08 Sep 2018 12:05:31: 28000000 INFO @ Sat, 08 Sep 2018 12:05:31: 28000000 INFO @ Sat, 08 Sep 2018 12:05:31: 28000000 INFO @ Sat, 08 Sep 2018 12:05:39: 29000000 INFO @ Sat, 08 Sep 2018 12:05:40: 29000000 INFO @ Sat, 08 Sep 2018 12:05:40: 29000000 INFO @ Sat, 08 Sep 2018 12:05:47: 30000000 INFO @ Sat, 08 Sep 2018 12:05:48: 30000000 INFO @ Sat, 08 Sep 2018 12:05:48: 30000000 INFO @ Sat, 08 Sep 2018 12:05:54: 31000000 INFO @ Sat, 08 Sep 2018 12:05:56: 31000000 INFO @ Sat, 08 Sep 2018 12:05:56: 31000000 INFO @ Sat, 08 Sep 2018 12:06:02: 32000000 INFO @ Sat, 08 Sep 2018 12:06:04: 32000000 INFO @ Sat, 08 Sep 2018 12:06:04: 32000000 INFO @ Sat, 08 Sep 2018 12:06:10: 33000000 INFO @ Sat, 08 Sep 2018 12:06:12: 33000000 INFO @ Sat, 08 Sep 2018 12:06:12: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:06:12: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:06:12: #1 total tags in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:12: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:06:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:06:12: 33000000 INFO @ Sat, 08 Sep 2018 12:06:13: #1 tags after filtering in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:06:13: #1 finished! INFO @ Sat, 08 Sep 2018 12:06:13: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:06:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:06:14: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:06:14: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:06:14: #1 total tags in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:14: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:06:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:06:14: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:06:14: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:06:14: #1 total tags in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:14: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:06:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:06:15: #1 tags after filtering in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:06:15: #1 finished! INFO @ Sat, 08 Sep 2018 12:06:15: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:06:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:06:15: #1 tags after filtering in treatment: 33228409 INFO @ Sat, 08 Sep 2018 12:06:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:06:15: #1 finished! INFO @ Sat, 08 Sep 2018 12:06:15: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:06:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:06:15: #2 number of paired peaks: 56 WARNING @ Sat, 08 Sep 2018 12:06:15: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:06:15: Process for pairing-model is terminated! cat: SRX2973387.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973387.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:06:17: #2 number of paired peaks: 56 WARNING @ Sat, 08 Sep 2018 12:06:17: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:06:17: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 12:06:17: #2 number of paired peaks: 56 WARNING @ Sat, 08 Sep 2018 12:06:17: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:06:17: Process for pairing-model is terminated! cat: SRX2973387.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2973387.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973387.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.20_model.r'rm: cannot remove `SRX2973387.10_*.xls': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973387.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。