Job ID = 10714362 sra ファイルのダウンロード中... Completed: 2322175K bytes transferred in 99 seconds (192092K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 45310834 spots for /home/okishinya/chipatlas/results/ce10/SRX2965743/SRR5766328.sra Written 45310834 spots for /home/okishinya/chipatlas/results/ce10/SRX2965743/SRR5766328.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:25 45310834 reads; of these: 45310834 (100.00%) were unpaired; of these: 9131268 (20.15%) aligned 0 times 31241498 (68.95%) aligned exactly 1 time 4938068 (10.90%) aligned >1 times 79.85% overall alignment rate Time searching: 00:19:25 Overall time: 00:19:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6696659 / 36179566 = 0.1851 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 03 Jun 2018 13:02:56: # Command line: callpeak -t SRX2965743.bam -f BAM -g ce -n SRX2965743.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2965743.05 # format = BAM # ChIP-seq file = ['SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:02:56: # Command line: callpeak -t SRX2965743.bam -f BAM -g ce -n SRX2965743.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2965743.10 # format = BAM # ChIP-seq file = ['SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:02:56: # Command line: callpeak -t SRX2965743.bam -f BAM -g ce -n SRX2965743.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2965743.20 # format = BAM # ChIP-seq file = ['SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:02:56: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:02:56: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:02:56: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:02:56: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:02:56: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:02:56: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:03:04: 1000000 INFO @ Sun, 03 Jun 2018 13:03:05: 1000000 INFO @ Sun, 03 Jun 2018 13:03:05: 1000000 INFO @ Sun, 03 Jun 2018 13:03:13: 2000000 INFO @ Sun, 03 Jun 2018 13:03:14: 2000000 INFO @ Sun, 03 Jun 2018 13:03:15: 2000000 INFO @ Sun, 03 Jun 2018 13:03:21: 3000000 INFO @ Sun, 03 Jun 2018 13:03:23: 3000000 INFO @ Sun, 03 Jun 2018 13:03:25: 3000000 INFO @ Sun, 03 Jun 2018 13:03:29: 4000000 INFO @ Sun, 03 Jun 2018 13:03:32: 4000000 INFO @ Sun, 03 Jun 2018 13:03:34: 4000000 INFO @ Sun, 03 Jun 2018 13:03:37: 5000000 INFO @ Sun, 03 Jun 2018 13:03:41: 5000000 INFO @ Sun, 03 Jun 2018 13:03:44: 5000000 INFO @ Sun, 03 Jun 2018 13:03:45: 6000000 INFO @ Sun, 03 Jun 2018 13:03:50: 6000000 INFO @ Sun, 03 Jun 2018 13:03:53: 7000000 INFO @ Sun, 03 Jun 2018 13:03:54: 6000000 INFO @ Sun, 03 Jun 2018 13:03:59: 7000000 INFO @ Sun, 03 Jun 2018 13:04:01: 8000000 INFO @ Sun, 03 Jun 2018 13:04:03: 7000000 INFO @ Sun, 03 Jun 2018 13:04:08: 8000000 INFO @ Sun, 03 Jun 2018 13:04:09: 9000000 INFO @ Sun, 03 Jun 2018 13:04:13: 8000000 INFO @ Sun, 03 Jun 2018 13:04:17: 10000000 INFO @ Sun, 03 Jun 2018 13:04:17: 9000000 INFO @ Sun, 03 Jun 2018 13:04:23: 9000000 INFO @ Sun, 03 Jun 2018 13:04:25: 11000000 INFO @ Sun, 03 Jun 2018 13:04:26: 10000000 INFO @ Sun, 03 Jun 2018 13:04:32: 10000000 INFO @ Sun, 03 Jun 2018 13:04:33: 12000000 INFO @ Sun, 03 Jun 2018 13:04:35: 11000000 INFO @ Sun, 03 Jun 2018 13:04:41: 13000000 INFO @ Sun, 03 Jun 2018 13:04:42: 11000000 INFO @ Sun, 03 Jun 2018 13:04:44: 12000000 INFO @ Sun, 03 Jun 2018 13:04:49: 14000000 INFO @ Sun, 03 Jun 2018 13:04:51: 12000000 INFO @ Sun, 03 Jun 2018 13:04:53: 13000000 INFO @ Sun, 03 Jun 2018 13:04:57: 15000000 INFO @ Sun, 03 Jun 2018 13:05:01: 13000000 INFO @ Sun, 03 Jun 2018 13:05:02: 14000000 INFO @ Sun, 03 Jun 2018 13:05:05: 16000000 INFO @ Sun, 03 Jun 2018 13:05:10: 14000000 INFO @ Sun, 03 Jun 2018 13:05:11: 15000000 INFO @ Sun, 03 Jun 2018 13:05:13: 17000000 INFO @ Sun, 03 Jun 2018 13:05:20: 16000000 INFO @ Sun, 03 Jun 2018 13:05:20: 15000000 INFO @ Sun, 03 Jun 2018 13:05:21: 18000000 INFO @ Sun, 03 Jun 2018 13:05:29: 19000000 INFO @ Sun, 03 Jun 2018 13:05:29: 17000000 INFO @ Sun, 03 Jun 2018 13:05:30: 16000000 INFO @ Sun, 03 Jun 2018 13:05:37: 20000000 INFO @ Sun, 03 Jun 2018 13:05:38: 18000000 INFO @ Sun, 03 Jun 2018 13:05:39: 17000000 INFO @ Sun, 03 Jun 2018 13:05:45: 21000000 INFO @ Sun, 03 Jun 2018 13:05:47: 19000000 INFO @ Sun, 03 Jun 2018 13:05:49: 18000000 INFO @ Sun, 03 Jun 2018 13:05:53: 22000000 INFO @ Sun, 03 Jun 2018 13:05:56: 20000000 INFO @ Sun, 03 Jun 2018 13:05:58: 19000000 INFO @ Sun, 03 Jun 2018 13:06:01: 23000000 INFO @ Sun, 03 Jun 2018 13:06:05: 21000000 INFO @ Sun, 03 Jun 2018 13:06:08: 20000000 INFO @ Sun, 03 Jun 2018 13:06:09: 24000000 INFO @ Sun, 03 Jun 2018 13:06:14: 22000000 INFO @ Sun, 03 Jun 2018 13:06:17: 25000000 INFO @ Sun, 03 Jun 2018 13:06:17: 21000000 INFO @ Sun, 03 Jun 2018 13:06:23: 23000000 INFO @ Sun, 03 Jun 2018 13:06:25: 26000000 INFO @ Sun, 03 Jun 2018 13:06:27: 22000000 INFO @ Sun, 03 Jun 2018 13:06:32: 24000000 INFO @ Sun, 03 Jun 2018 13:06:33: 27000000 INFO @ Sun, 03 Jun 2018 13:06:37: 23000000 INFO @ Sun, 03 Jun 2018 13:06:41: 25000000 INFO @ Sun, 03 Jun 2018 13:06:41: 28000000 INFO @ Sun, 03 Jun 2018 13:06:46: 24000000 INFO @ Sun, 03 Jun 2018 13:06:49: 29000000 INFO @ Sun, 03 Jun 2018 13:06:50: 26000000 INFO @ Sun, 03 Jun 2018 13:06:53: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 13:06:53: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 13:06:53: #1 total tags in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:06:53: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:06:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:06:53: #1 tags after filtering in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:06:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:06:53: #1 finished! INFO @ Sun, 03 Jun 2018 13:06:53: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:06:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:06:55: #2 number of paired peaks: 49 WARNING @ Sun, 03 Jun 2018 13:06:55: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:06:55: Process for pairing-model is terminated! cat: SRX2965743.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 24 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965743.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 13:06:56: 25000000 INFO @ Sun, 03 Jun 2018 13:06:58: 27000000 INFO @ Sun, 03 Jun 2018 13:07:05: 26000000 INFO @ Sun, 03 Jun 2018 13:07:07: 28000000 INFO @ Sun, 03 Jun 2018 13:07:15: 27000000 INFO @ Sun, 03 Jun 2018 13:07:16: 29000000 INFO @ Sun, 03 Jun 2018 13:07:21: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 13:07:21: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 13:07:21: #1 total tags in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:07:21: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:07:21: #1 tags after filtering in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:07:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:07:21: #1 finished! INFO @ Sun, 03 Jun 2018 13:07:21: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:07:23: #2 number of paired peaks: 49 WARNING @ Sun, 03 Jun 2018 13:07:23: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:07:23: Process for pairing-model is terminated! cat: SRX2965743.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965743.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 13:07:24: 28000000 INFO @ Sun, 03 Jun 2018 13:07:32: 29000000 INFO @ Sun, 03 Jun 2018 13:07:36: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 13:07:36: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 13:07:36: #1 total tags in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:07:36: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:07:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:07:37: #1 tags after filtering in treatment: 29482907 INFO @ Sun, 03 Jun 2018 13:07:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:07:37: #1 finished! INFO @ Sun, 03 Jun 2018 13:07:37: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:07:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:07:39: #2 number of paired peaks: 49 WARNING @ Sun, 03 Jun 2018 13:07:39: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:07:39: Process for pairing-model is terminated! cat: SRX2965743.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965743.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965743.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。