Job ID = 11170812 sra ファイルのダウンロード中... Completed: 1424520K bytes transferred in 198 seconds (58814K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 49540971 spots for /home/okishinya/chipatlas/results/ce10/SRX2965737/SRR5766322.sra Written 49540971 spots for /home/okishinya/chipatlas/results/ce10/SRX2965737/SRR5766322.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:19 49540971 reads; of these: 49540971 (100.00%) were unpaired; of these: 4122621 (8.32%) aligned 0 times 38470291 (77.65%) aligned exactly 1 time 6948059 (14.02%) aligned >1 times 91.68% overall alignment rate Time searching: 00:19:19 Overall time: 00:19:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12189394 / 45418350 = 0.2684 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:01:33: # Command line: callpeak -t SRX2965737.bam -f BAM -g ce -n SRX2965737.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2965737.20 # format = BAM # ChIP-seq file = ['SRX2965737.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:33: # Command line: callpeak -t SRX2965737.bam -f BAM -g ce -n SRX2965737.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2965737.10 # format = BAM # ChIP-seq file = ['SRX2965737.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:33: # Command line: callpeak -t SRX2965737.bam -f BAM -g ce -n SRX2965737.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2965737.05 # format = BAM # ChIP-seq file = ['SRX2965737.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:01:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:01:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:01:41: 1000000 INFO @ Sat, 08 Sep 2018 12:01:41: 1000000 INFO @ Sat, 08 Sep 2018 12:01:41: 1000000 INFO @ Sat, 08 Sep 2018 12:01:48: 2000000 INFO @ Sat, 08 Sep 2018 12:01:49: 2000000 INFO @ Sat, 08 Sep 2018 12:01:49: 2000000 INFO @ Sat, 08 Sep 2018 12:01:56: 3000000 INFO @ Sat, 08 Sep 2018 12:01:56: 3000000 INFO @ Sat, 08 Sep 2018 12:01:56: 3000000 INFO @ Sat, 08 Sep 2018 12:02:04: 4000000 INFO @ Sat, 08 Sep 2018 12:02:04: 4000000 INFO @ Sat, 08 Sep 2018 12:02:04: 4000000 INFO @ Sat, 08 Sep 2018 12:02:11: 5000000 INFO @ Sat, 08 Sep 2018 12:02:12: 5000000 INFO @ Sat, 08 Sep 2018 12:02:12: 5000000 INFO @ Sat, 08 Sep 2018 12:02:19: 6000000 INFO @ Sat, 08 Sep 2018 12:02:20: 6000000 INFO @ Sat, 08 Sep 2018 12:02:20: 6000000 INFO @ Sat, 08 Sep 2018 12:02:27: 7000000 INFO @ Sat, 08 Sep 2018 12:02:28: 7000000 INFO @ Sat, 08 Sep 2018 12:02:28: 7000000 INFO @ Sat, 08 Sep 2018 12:02:35: 8000000 INFO @ Sat, 08 Sep 2018 12:02:36: 8000000 INFO @ Sat, 08 Sep 2018 12:02:36: 8000000 INFO @ Sat, 08 Sep 2018 12:02:42: 9000000 INFO @ Sat, 08 Sep 2018 12:02:43: 9000000 INFO @ Sat, 08 Sep 2018 12:02:43: 9000000 INFO @ Sat, 08 Sep 2018 12:02:50: 10000000 INFO @ Sat, 08 Sep 2018 12:02:51: 10000000 INFO @ Sat, 08 Sep 2018 12:02:51: 10000000 INFO @ Sat, 08 Sep 2018 12:02:58: 11000000 INFO @ Sat, 08 Sep 2018 12:02:59: 11000000 INFO @ Sat, 08 Sep 2018 12:02:59: 11000000 INFO @ Sat, 08 Sep 2018 12:03:05: 12000000 INFO @ Sat, 08 Sep 2018 12:03:07: 12000000 INFO @ Sat, 08 Sep 2018 12:03:07: 12000000 INFO @ Sat, 08 Sep 2018 12:03:13: 13000000 INFO @ Sat, 08 Sep 2018 12:03:15: 13000000 INFO @ Sat, 08 Sep 2018 12:03:15: 13000000 INFO @ Sat, 08 Sep 2018 12:03:21: 14000000 INFO @ Sat, 08 Sep 2018 12:03:23: 14000000 INFO @ Sat, 08 Sep 2018 12:03:23: 14000000 INFO @ Sat, 08 Sep 2018 12:03:29: 15000000 INFO @ Sat, 08 Sep 2018 12:03:31: 15000000 INFO @ Sat, 08 Sep 2018 12:03:31: 15000000 INFO @ Sat, 08 Sep 2018 12:03:37: 16000000 INFO @ Sat, 08 Sep 2018 12:03:39: 16000000 INFO @ Sat, 08 Sep 2018 12:03:40: 16000000 INFO @ Sat, 08 Sep 2018 12:03:46: 17000000 INFO @ Sat, 08 Sep 2018 12:03:48: 17000000 INFO @ Sat, 08 Sep 2018 12:03:48: 17000000 INFO @ Sat, 08 Sep 2018 12:03:54: 18000000 INFO @ Sat, 08 Sep 2018 12:03:56: 18000000 INFO @ Sat, 08 Sep 2018 12:03:56: 18000000 INFO @ Sat, 08 Sep 2018 12:04:02: 19000000 INFO @ Sat, 08 Sep 2018 12:04:04: 19000000 INFO @ Sat, 08 Sep 2018 12:04:04: 19000000 INFO @ Sat, 08 Sep 2018 12:04:10: 20000000 INFO @ Sat, 08 Sep 2018 12:04:12: 20000000 INFO @ Sat, 08 Sep 2018 12:04:12: 20000000 INFO @ Sat, 08 Sep 2018 12:04:18: 21000000 INFO @ Sat, 08 Sep 2018 12:04:20: 21000000 INFO @ Sat, 08 Sep 2018 12:04:20: 21000000 INFO @ Sat, 08 Sep 2018 12:04:26: 22000000 INFO @ Sat, 08 Sep 2018 12:04:28: 22000000 INFO @ Sat, 08 Sep 2018 12:04:28: 22000000 INFO @ Sat, 08 Sep 2018 12:04:33: 23000000 INFO @ Sat, 08 Sep 2018 12:04:36: 23000000 INFO @ Sat, 08 Sep 2018 12:04:36: 23000000 INFO @ Sat, 08 Sep 2018 12:04:41: 24000000 INFO @ Sat, 08 Sep 2018 12:04:44: 24000000 INFO @ Sat, 08 Sep 2018 12:04:44: 24000000 INFO @ Sat, 08 Sep 2018 12:04:49: 25000000 INFO @ Sat, 08 Sep 2018 12:04:52: 25000000 INFO @ Sat, 08 Sep 2018 12:04:53: 25000000 INFO @ Sat, 08 Sep 2018 12:04:57: 26000000 INFO @ Sat, 08 Sep 2018 12:05:01: 26000000 INFO @ Sat, 08 Sep 2018 12:05:01: 26000000 INFO @ Sat, 08 Sep 2018 12:05:05: 27000000 INFO @ Sat, 08 Sep 2018 12:05:09: 27000000 INFO @ Sat, 08 Sep 2018 12:05:09: 27000000 INFO @ Sat, 08 Sep 2018 12:05:13: 28000000 INFO @ Sat, 08 Sep 2018 12:05:17: 28000000 INFO @ Sat, 08 Sep 2018 12:05:17: 28000000 INFO @ Sat, 08 Sep 2018 12:05:20: 29000000 INFO @ Sat, 08 Sep 2018 12:05:25: 29000000 INFO @ Sat, 08 Sep 2018 12:05:25: 29000000 INFO @ Sat, 08 Sep 2018 12:05:28: 30000000 INFO @ Sat, 08 Sep 2018 12:05:33: 30000000 INFO @ Sat, 08 Sep 2018 12:05:33: 30000000 INFO @ Sat, 08 Sep 2018 12:05:36: 31000000 INFO @ Sat, 08 Sep 2018 12:05:41: 31000000 INFO @ Sat, 08 Sep 2018 12:05:41: 31000000 INFO @ Sat, 08 Sep 2018 12:05:43: 32000000 INFO @ Sat, 08 Sep 2018 12:05:49: 32000000 INFO @ Sat, 08 Sep 2018 12:05:49: 32000000 INFO @ Sat, 08 Sep 2018 12:05:51: 33000000 INFO @ Sat, 08 Sep 2018 12:05:53: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:05:53: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:05:53: #1 total tags in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:05:53: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:05:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:05:54: #1 tags after filtering in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:05:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:05:54: #1 finished! INFO @ Sat, 08 Sep 2018 12:05:54: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:05:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:05:56: #2 number of paired peaks: 55 WARNING @ Sat, 08 Sep 2018 12:05:56: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:05:56: Process for pairing-model is terminated! cat: SRX2965737.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965737.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:05:57: 33000000 INFO @ Sat, 08 Sep 2018 12:05:57: 33000000 INFO @ Sat, 08 Sep 2018 12:05:59: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:05:59: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:05:59: #1 total tags in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:05:59: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:05:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:05:59: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:05:59: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:05:59: #1 total tags in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:05:59: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:05:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:06:00: #1 tags after filtering in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:06:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:06:00: #1 finished! INFO @ Sat, 08 Sep 2018 12:06:00: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:06:00: #1 tags after filtering in treatment: 33228956 INFO @ Sat, 08 Sep 2018 12:06:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:06:00: #1 finished! INFO @ Sat, 08 Sep 2018 12:06:00: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:06:02: #2 number of paired peaks: 55 WARNING @ Sat, 08 Sep 2018 12:06:02: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:06:02: Process for pairing-model is terminated! cat: SRX2965737.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965737.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:06:02: #2 number of paired peaks: 55 WARNING @ Sat, 08 Sep 2018 12:06:02: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:06:02: Process for pairing-model is terminated! cat: SRX2965737.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965737.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965737.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。