Job ID = 11170809 sra ファイルのダウンロード中... Completed: 1899410K bytes transferred in 142 seconds (108818K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 70079354 spots for /home/okishinya/chipatlas/results/ce10/SRX2965734/SRR5766319.sra Written 70079354 spots for /home/okishinya/chipatlas/results/ce10/SRX2965734/SRR5766319.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:01 70079354 reads; of these: 70079354 (100.00%) were unpaired; of these: 11799151 (16.84%) aligned 0 times 48415460 (69.09%) aligned exactly 1 time 9864743 (14.08%) aligned >1 times 83.16% overall alignment rate Time searching: 00:25:01 Overall time: 00:25:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 20370882 / 58280203 = 0.3495 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:10:10: # Command line: callpeak -t SRX2965734.bam -f BAM -g ce -n SRX2965734.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2965734.10 # format = BAM # ChIP-seq file = ['SRX2965734.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:10:10: # Command line: callpeak -t SRX2965734.bam -f BAM -g ce -n SRX2965734.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2965734.05 # format = BAM # ChIP-seq file = ['SRX2965734.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:10:10: # Command line: callpeak -t SRX2965734.bam -f BAM -g ce -n SRX2965734.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2965734.20 # format = BAM # ChIP-seq file = ['SRX2965734.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:10:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:10:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:10:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:10:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:10:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:10:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:10:18: 1000000 INFO @ Sat, 08 Sep 2018 12:10:19: 1000000 INFO @ Sat, 08 Sep 2018 12:10:19: 1000000 INFO @ Sat, 08 Sep 2018 12:10:25: 2000000 INFO @ Sat, 08 Sep 2018 12:10:27: 2000000 INFO @ Sat, 08 Sep 2018 12:10:27: 2000000 INFO @ Sat, 08 Sep 2018 12:10:32: 3000000 INFO @ Sat, 08 Sep 2018 12:10:35: 3000000 INFO @ Sat, 08 Sep 2018 12:10:35: 3000000 INFO @ Sat, 08 Sep 2018 12:10:40: 4000000 INFO @ Sat, 08 Sep 2018 12:10:44: 4000000 INFO @ Sat, 08 Sep 2018 12:10:44: 4000000 INFO @ Sat, 08 Sep 2018 12:10:47: 5000000 INFO @ Sat, 08 Sep 2018 12:10:52: 5000000 INFO @ Sat, 08 Sep 2018 12:10:52: 5000000 INFO @ Sat, 08 Sep 2018 12:10:54: 6000000 INFO @ Sat, 08 Sep 2018 12:11:00: 6000000 INFO @ Sat, 08 Sep 2018 12:11:00: 6000000 INFO @ Sat, 08 Sep 2018 12:11:01: 7000000 INFO @ Sat, 08 Sep 2018 12:11:09: 8000000 INFO @ Sat, 08 Sep 2018 12:11:09: 7000000 INFO @ Sat, 08 Sep 2018 12:11:09: 7000000 INFO @ Sat, 08 Sep 2018 12:11:16: 9000000 INFO @ Sat, 08 Sep 2018 12:11:17: 8000000 INFO @ Sat, 08 Sep 2018 12:11:17: 8000000 INFO @ Sat, 08 Sep 2018 12:11:23: 10000000 INFO @ Sat, 08 Sep 2018 12:11:25: 9000000 INFO @ Sat, 08 Sep 2018 12:11:25: 9000000 INFO @ Sat, 08 Sep 2018 12:11:30: 11000000 INFO @ Sat, 08 Sep 2018 12:11:33: 10000000 INFO @ Sat, 08 Sep 2018 12:11:33: 10000000 INFO @ Sat, 08 Sep 2018 12:11:37: 12000000 INFO @ Sat, 08 Sep 2018 12:11:42: 11000000 INFO @ Sat, 08 Sep 2018 12:11:42: 11000000 INFO @ Sat, 08 Sep 2018 12:11:45: 13000000 INFO @ Sat, 08 Sep 2018 12:11:50: 12000000 INFO @ Sat, 08 Sep 2018 12:11:50: 12000000 INFO @ Sat, 08 Sep 2018 12:11:52: 14000000 INFO @ Sat, 08 Sep 2018 12:11:58: 13000000 INFO @ Sat, 08 Sep 2018 12:11:58: 13000000 INFO @ Sat, 08 Sep 2018 12:11:59: 15000000 INFO @ Sat, 08 Sep 2018 12:12:06: 16000000 INFO @ Sat, 08 Sep 2018 12:12:07: 14000000 INFO @ Sat, 08 Sep 2018 12:12:07: 14000000 INFO @ Sat, 08 Sep 2018 12:12:14: 17000000 INFO @ Sat, 08 Sep 2018 12:12:15: 15000000 INFO @ Sat, 08 Sep 2018 12:12:15: 15000000 INFO @ Sat, 08 Sep 2018 12:12:21: 18000000 INFO @ Sat, 08 Sep 2018 12:12:23: 16000000 INFO @ Sat, 08 Sep 2018 12:12:23: 16000000 INFO @ Sat, 08 Sep 2018 12:12:28: 19000000 INFO @ Sat, 08 Sep 2018 12:12:32: 17000000 INFO @ Sat, 08 Sep 2018 12:12:32: 17000000 INFO @ Sat, 08 Sep 2018 12:12:35: 20000000 INFO @ Sat, 08 Sep 2018 12:12:40: 18000000 INFO @ Sat, 08 Sep 2018 12:12:40: 18000000 INFO @ Sat, 08 Sep 2018 12:12:43: 21000000 INFO @ Sat, 08 Sep 2018 12:12:48: 19000000 INFO @ Sat, 08 Sep 2018 12:12:48: 19000000 INFO @ Sat, 08 Sep 2018 12:12:50: 22000000 INFO @ Sat, 08 Sep 2018 12:12:56: 20000000 INFO @ Sat, 08 Sep 2018 12:12:56: 20000000 INFO @ Sat, 08 Sep 2018 12:12:57: 23000000 INFO @ Sat, 08 Sep 2018 12:13:04: 24000000 INFO @ Sat, 08 Sep 2018 12:13:05: 21000000 INFO @ Sat, 08 Sep 2018 12:13:05: 21000000 INFO @ Sat, 08 Sep 2018 12:13:12: 25000000 INFO @ Sat, 08 Sep 2018 12:13:13: 22000000 INFO @ Sat, 08 Sep 2018 12:13:13: 22000000 INFO @ Sat, 08 Sep 2018 12:13:19: 26000000 INFO @ Sat, 08 Sep 2018 12:13:21: 23000000 INFO @ Sat, 08 Sep 2018 12:13:21: 23000000 INFO @ Sat, 08 Sep 2018 12:13:26: 27000000 INFO @ Sat, 08 Sep 2018 12:13:29: 24000000 INFO @ Sat, 08 Sep 2018 12:13:29: 24000000 INFO @ Sat, 08 Sep 2018 12:13:33: 28000000 INFO @ Sat, 08 Sep 2018 12:13:38: 25000000 INFO @ Sat, 08 Sep 2018 12:13:38: 25000000 INFO @ Sat, 08 Sep 2018 12:13:40: 29000000 INFO @ Sat, 08 Sep 2018 12:13:46: 26000000 INFO @ Sat, 08 Sep 2018 12:13:46: 26000000 INFO @ Sat, 08 Sep 2018 12:13:48: 30000000 INFO @ Sat, 08 Sep 2018 12:13:54: 27000000 INFO @ Sat, 08 Sep 2018 12:13:54: 27000000 INFO @ Sat, 08 Sep 2018 12:13:55: 31000000 INFO @ Sat, 08 Sep 2018 12:14:02: 32000000 INFO @ Sat, 08 Sep 2018 12:14:02: 28000000 INFO @ Sat, 08 Sep 2018 12:14:02: 28000000 INFO @ Sat, 08 Sep 2018 12:14:09: 33000000 INFO @ Sat, 08 Sep 2018 12:14:10: 29000000 INFO @ Sat, 08 Sep 2018 12:14:10: 29000000 INFO @ Sat, 08 Sep 2018 12:14:16: 34000000 INFO @ Sat, 08 Sep 2018 12:14:19: 30000000 INFO @ Sat, 08 Sep 2018 12:14:19: 30000000 INFO @ Sat, 08 Sep 2018 12:14:24: 35000000 INFO @ Sat, 08 Sep 2018 12:14:27: 31000000 INFO @ Sat, 08 Sep 2018 12:14:27: 31000000 INFO @ Sat, 08 Sep 2018 12:14:31: 36000000 INFO @ Sat, 08 Sep 2018 12:14:35: 32000000 INFO @ Sat, 08 Sep 2018 12:14:35: 32000000 INFO @ Sat, 08 Sep 2018 12:14:38: 37000000 INFO @ Sat, 08 Sep 2018 12:14:43: 33000000 INFO @ Sat, 08 Sep 2018 12:14:43: 33000000 INFO @ Sat, 08 Sep 2018 12:14:45: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:14:45: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:14:45: #1 total tags in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:14:45: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:14:46: #1 tags after filtering in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:14:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:14:46: #1 finished! INFO @ Sat, 08 Sep 2018 12:14:46: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:14:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:14:48: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:14:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:14:48: Process for pairing-model is terminated! cat: SRX2965734.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 17 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965734.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:14:51: 34000000 INFO @ Sat, 08 Sep 2018 12:14:51: 34000000 INFO @ Sat, 08 Sep 2018 12:14:59: 35000000 INFO @ Sat, 08 Sep 2018 12:14:59: 35000000 INFO @ Sat, 08 Sep 2018 12:15:06: 36000000 INFO @ Sat, 08 Sep 2018 12:15:06: 36000000 INFO @ Sat, 08 Sep 2018 12:15:13: 37000000 INFO @ Sat, 08 Sep 2018 12:15:14: 37000000 INFO @ Sat, 08 Sep 2018 12:15:20: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:15:20: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:15:20: #1 total tags in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:15:20: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:15:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:15:21: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:15:21: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:15:21: #1 total tags in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:15:21: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:15:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:15:21: #1 tags after filtering in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:15:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:15:21: #1 finished! INFO @ Sat, 08 Sep 2018 12:15:21: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:15:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:15:22: #1 tags after filtering in treatment: 37909321 INFO @ Sat, 08 Sep 2018 12:15:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:15:22: #1 finished! INFO @ Sat, 08 Sep 2018 12:15:22: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:15:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:15:23: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:15:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:15:23: Process for pairing-model is terminated! cat: SRX2965734.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965734.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:15:24: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:15:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:15:24: Process for pairing-model is terminated! cat: SRX2965734.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2965734.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2965734.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。