Job ID = 2589655 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,246,825 reads read : 4,246,825 reads written : 4,246,825 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:51 4246825 reads; of these: 4246825 (100.00%) were unpaired; of these: 103763 (2.44%) aligned 0 times 3483247 (82.02%) aligned exactly 1 time 659815 (15.54%) aligned >1 times 97.56% overall alignment rate Time searching: 00:00:51 Overall time: 00:00:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 240488 / 4143062 = 0.0580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:12:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:12:36: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:12:36: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:12:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:12:37: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:12:37: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:12:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:12:38: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:12:38: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:12:44: 1000000 INFO @ Mon, 12 Aug 2019 18:12:45: 1000000 INFO @ Mon, 12 Aug 2019 18:12:47: 1000000 INFO @ Mon, 12 Aug 2019 18:12:52: 2000000 INFO @ Mon, 12 Aug 2019 18:12:53: 2000000 INFO @ Mon, 12 Aug 2019 18:12:57: 2000000 INFO @ Mon, 12 Aug 2019 18:13:00: 3000000 INFO @ Mon, 12 Aug 2019 18:13:01: 3000000 INFO @ Mon, 12 Aug 2019 18:13:06: 3000000 INFO @ Mon, 12 Aug 2019 18:13:07: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:13:07: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:13:07: #1 total tags in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:07: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:13:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:13:07: #1 tags after filtering in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:13:07: #1 finished! INFO @ Mon, 12 Aug 2019 18:13:07: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:13:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:13:08: #2 number of paired peaks: 385 WARNING @ Mon, 12 Aug 2019 18:13:08: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Mon, 12 Aug 2019 18:13:08: start model_add_line... INFO @ Mon, 12 Aug 2019 18:13:08: start X-correlation... INFO @ Mon, 12 Aug 2019 18:13:08: end of X-cor INFO @ Mon, 12 Aug 2019 18:13:08: #2 finished! INFO @ Mon, 12 Aug 2019 18:13:08: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:13:08: #2 alternative fragment length(s) may be 4,31,491,597 bps INFO @ Mon, 12 Aug 2019 18:13:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05_model.r WARNING @ Mon, 12 Aug 2019 18:13:08: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:13:08: #2 You may need to consider one of the other alternative d(s): 4,31,491,597 WARNING @ Mon, 12 Aug 2019 18:13:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:13:08: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:13:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:13:08: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:13:08: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:13:08: #1 total tags in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:13:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:13:08: #1 tags after filtering in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:13:08: #1 finished! INFO @ Mon, 12 Aug 2019 18:13:08: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:13:09: #2 number of paired peaks: 385 WARNING @ Mon, 12 Aug 2019 18:13:09: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Mon, 12 Aug 2019 18:13:09: start model_add_line... INFO @ Mon, 12 Aug 2019 18:13:09: start X-correlation... INFO @ Mon, 12 Aug 2019 18:13:09: end of X-cor INFO @ Mon, 12 Aug 2019 18:13:09: #2 finished! INFO @ Mon, 12 Aug 2019 18:13:09: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:13:09: #2 alternative fragment length(s) may be 4,31,491,597 bps INFO @ Mon, 12 Aug 2019 18:13:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10_model.r WARNING @ Mon, 12 Aug 2019 18:13:09: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:13:09: #2 You may need to consider one of the other alternative d(s): 4,31,491,597 WARNING @ Mon, 12 Aug 2019 18:13:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:13:09: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:13:09: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:13:13: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:13:13: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:13:13: #1 total tags in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:13: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:13:13: #1 tags after filtering in treatment: 3902574 INFO @ Mon, 12 Aug 2019 18:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:13:13: #1 finished! INFO @ Mon, 12 Aug 2019 18:13:13: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:13:14: #2 number of paired peaks: 385 WARNING @ Mon, 12 Aug 2019 18:13:14: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Mon, 12 Aug 2019 18:13:14: start model_add_line... INFO @ Mon, 12 Aug 2019 18:13:14: start X-correlation... INFO @ Mon, 12 Aug 2019 18:13:14: end of X-cor INFO @ Mon, 12 Aug 2019 18:13:14: #2 finished! INFO @ Mon, 12 Aug 2019 18:13:14: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:13:14: #2 alternative fragment length(s) may be 4,31,491,597 bps INFO @ Mon, 12 Aug 2019 18:13:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20_model.r WARNING @ Mon, 12 Aug 2019 18:13:14: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:13:14: #2 You may need to consider one of the other alternative d(s): 4,31,491,597 WARNING @ Mon, 12 Aug 2019 18:13:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:13:14: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:13:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:13:20: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:13:20: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:13:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:13:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:13:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.05_summits.bed INFO @ Mon, 12 Aug 2019 18:13:25: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (344 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:13:26: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:13:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:13:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:13:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.10_summits.bed INFO @ Mon, 12 Aug 2019 18:13:26: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (168 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:13:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:13:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:13:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277099/SRX277099.20_summits.bed INFO @ Mon, 12 Aug 2019 18:13:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (56 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。