Job ID = 2589580 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,786,149 reads read : 4,786,149 reads written : 4,786,149 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:49 4786149 reads; of these: 4786149 (100.00%) were unpaired; of these: 734961 (15.36%) aligned 0 times 3399859 (71.04%) aligned exactly 1 time 651329 (13.61%) aligned >1 times 84.64% overall alignment rate Time searching: 00:00:49 Overall time: 00:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 217315 / 4051188 = 0.0536 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:02:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:30: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:30: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:02:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:31: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:31: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:02:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:02:38: 1000000 INFO @ Mon, 12 Aug 2019 18:02:40: 1000000 INFO @ Mon, 12 Aug 2019 18:02:42: 1000000 INFO @ Mon, 12 Aug 2019 18:02:45: 2000000 INFO @ Mon, 12 Aug 2019 18:02:49: 2000000 INFO @ Mon, 12 Aug 2019 18:02:51: 2000000 INFO @ Mon, 12 Aug 2019 18:02:52: 3000000 INFO @ Mon, 12 Aug 2019 18:02:58: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:02:58: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:02:58: #1 total tags in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:02:58: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:02:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:02:58: #1 tags after filtering in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:02:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:02:58: #1 finished! INFO @ Mon, 12 Aug 2019 18:02:58: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:02:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:02:58: #2 number of paired peaks: 378 WARNING @ Mon, 12 Aug 2019 18:02:58: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Mon, 12 Aug 2019 18:02:58: start model_add_line... INFO @ Mon, 12 Aug 2019 18:02:58: start X-correlation... INFO @ Mon, 12 Aug 2019 18:02:58: end of X-cor INFO @ Mon, 12 Aug 2019 18:02:58: #2 finished! INFO @ Mon, 12 Aug 2019 18:02:58: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:02:58: #2 alternative fragment length(s) may be 3,31,529,570 bps INFO @ Mon, 12 Aug 2019 18:02:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10_model.r WARNING @ Mon, 12 Aug 2019 18:02:58: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:02:58: #2 You may need to consider one of the other alternative d(s): 3,31,529,570 WARNING @ Mon, 12 Aug 2019 18:02:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:02:58: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:02:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:02:59: 3000000 INFO @ Mon, 12 Aug 2019 18:03:01: 3000000 INFO @ Mon, 12 Aug 2019 18:03:06: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:03:06: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:03:06: #1 total tags in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:03:06: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:03:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:03:06: #1 tags after filtering in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:03:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:03:06: #1 finished! INFO @ Mon, 12 Aug 2019 18:03:06: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:03:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:03:06: #2 number of paired peaks: 378 WARNING @ Mon, 12 Aug 2019 18:03:06: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Mon, 12 Aug 2019 18:03:06: start model_add_line... INFO @ Mon, 12 Aug 2019 18:03:06: start X-correlation... INFO @ Mon, 12 Aug 2019 18:03:06: end of X-cor INFO @ Mon, 12 Aug 2019 18:03:06: #2 finished! INFO @ Mon, 12 Aug 2019 18:03:06: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:03:06: #2 alternative fragment length(s) may be 3,31,529,570 bps INFO @ Mon, 12 Aug 2019 18:03:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05_model.r WARNING @ Mon, 12 Aug 2019 18:03:06: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:03:06: #2 You may need to consider one of the other alternative d(s): 3,31,529,570 WARNING @ Mon, 12 Aug 2019 18:03:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:03:06: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:03:06: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:03:08: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:03:08: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:03:08: #1 total tags in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:03:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:03:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:03:08: #1 tags after filtering in treatment: 3833873 INFO @ Mon, 12 Aug 2019 18:03:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:03:08: #1 finished! INFO @ Mon, 12 Aug 2019 18:03:08: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:03:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:03:08: #2 number of paired peaks: 378 WARNING @ Mon, 12 Aug 2019 18:03:08: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Mon, 12 Aug 2019 18:03:08: start model_add_line... INFO @ Mon, 12 Aug 2019 18:03:08: start X-correlation... INFO @ Mon, 12 Aug 2019 18:03:08: end of X-cor INFO @ Mon, 12 Aug 2019 18:03:08: #2 finished! INFO @ Mon, 12 Aug 2019 18:03:08: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 18:03:08: #2 alternative fragment length(s) may be 3,31,529,570 bps INFO @ Mon, 12 Aug 2019 18:03:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20_model.r WARNING @ Mon, 12 Aug 2019 18:03:08: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:03:08: #2 You may need to consider one of the other alternative d(s): 3,31,529,570 WARNING @ Mon, 12 Aug 2019 18:03:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:03:08: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:03:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:03:10: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.10_summits.bed INFO @ Mon, 12 Aug 2019 18:03:15: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (182 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:03:18: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:19: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.05_summits.bed INFO @ Mon, 12 Aug 2019 18:03:23: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (355 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:03:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276914/SRX276914.20_summits.bed INFO @ Mon, 12 Aug 2019 18:03:25: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (55 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。