Job ID = 9157295 sra ファイルのダウンロード中... Completed: 864148K bytes transferred in 20 seconds (349653K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 17765196 spots for /home/okishinya/chipatlas/results/ce10/SRX2761386/SRR5476960.sra Written 17765196 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 17765196 reads; of these: 17765196 (100.00%) were unpaired; of these: 852105 (4.80%) aligned 0 times 12667443 (71.30%) aligned exactly 1 time 4245648 (23.90%) aligned >1 times 95.20% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2295012 / 16913091 = 0.1357 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:31:45: # Command line: callpeak -t SRX2761386.bam -f BAM -g ce -n SRX2761386.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2761386.05 # format = BAM # ChIP-seq file = ['SRX2761386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:31:45: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:31:45: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:31:45: # Command line: callpeak -t SRX2761386.bam -f BAM -g ce -n SRX2761386.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2761386.20 # format = BAM # ChIP-seq file = ['SRX2761386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:31:45: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:31:45: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:31:45: # Command line: callpeak -t SRX2761386.bam -f BAM -g ce -n SRX2761386.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2761386.10 # format = BAM # ChIP-seq file = ['SRX2761386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:31:45: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:31:45: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:31:52: 1000000 INFO @ Tue, 27 Jun 2017 11:31:52: 1000000 INFO @ Tue, 27 Jun 2017 11:31:52: 1000000 INFO @ Tue, 27 Jun 2017 11:31:58: 2000000 INFO @ Tue, 27 Jun 2017 11:31:58: 2000000 INFO @ Tue, 27 Jun 2017 11:31:59: 2000000 INFO @ Tue, 27 Jun 2017 11:32:04: 3000000 INFO @ Tue, 27 Jun 2017 11:32:05: 3000000 INFO @ Tue, 27 Jun 2017 11:32:06: 3000000 INFO @ Tue, 27 Jun 2017 11:32:11: 4000000 INFO @ Tue, 27 Jun 2017 11:32:11: 4000000 INFO @ Tue, 27 Jun 2017 11:32:14: 4000000 INFO @ Tue, 27 Jun 2017 11:32:17: 5000000 INFO @ Tue, 27 Jun 2017 11:32:17: 5000000 INFO @ Tue, 27 Jun 2017 11:32:21: 5000000 INFO @ Tue, 27 Jun 2017 11:32:24: 6000000 INFO @ Tue, 27 Jun 2017 11:32:24: 6000000 INFO @ Tue, 27 Jun 2017 11:32:29: 6000000 INFO @ Tue, 27 Jun 2017 11:32:30: 7000000 INFO @ Tue, 27 Jun 2017 11:32:30: 7000000 INFO @ Tue, 27 Jun 2017 11:32:36: 7000000 INFO @ Tue, 27 Jun 2017 11:32:37: 8000000 INFO @ Tue, 27 Jun 2017 11:32:37: 8000000 INFO @ Tue, 27 Jun 2017 11:32:43: 8000000 INFO @ Tue, 27 Jun 2017 11:32:43: 9000000 INFO @ Tue, 27 Jun 2017 11:32:44: 9000000 INFO @ Tue, 27 Jun 2017 11:32:50: 9000000 INFO @ Tue, 27 Jun 2017 11:32:50: 10000000 INFO @ Tue, 27 Jun 2017 11:32:50: 10000000 INFO @ Tue, 27 Jun 2017 11:32:57: 11000000 INFO @ Tue, 27 Jun 2017 11:32:57: 10000000 INFO @ Tue, 27 Jun 2017 11:32:57: 11000000 INFO @ Tue, 27 Jun 2017 11:33:03: 12000000 INFO @ Tue, 27 Jun 2017 11:33:04: 12000000 INFO @ Tue, 27 Jun 2017 11:33:04: 11000000 INFO @ Tue, 27 Jun 2017 11:33:10: 13000000 INFO @ Tue, 27 Jun 2017 11:33:11: 13000000 INFO @ Tue, 27 Jun 2017 11:33:12: 12000000 INFO @ Tue, 27 Jun 2017 11:33:17: 14000000 INFO @ Tue, 27 Jun 2017 11:33:17: 14000000 INFO @ Tue, 27 Jun 2017 11:33:19: 13000000 INFO @ Tue, 27 Jun 2017 11:33:21: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:33:21: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:33:21: #1 total tags in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:21: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:33:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:33:21: #1 tags after filtering in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:33:21: #1 finished! INFO @ Tue, 27 Jun 2017 11:33:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:33:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:33:22: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:33:22: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:33:22: #1 total tags in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:22: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:33:22: #1 tags after filtering in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:33:22: #1 finished! INFO @ Tue, 27 Jun 2017 11:33:22: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:33:22: #2 number of paired peaks: 496 WARNING @ Tue, 27 Jun 2017 11:33:22: Fewer paired peaks (496) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 496 pairs to build model! INFO @ Tue, 27 Jun 2017 11:33:22: start model_add_line... INFO @ Tue, 27 Jun 2017 11:33:22: start X-correlation... INFO @ Tue, 27 Jun 2017 11:33:22: end of X-cor INFO @ Tue, 27 Jun 2017 11:33:22: #2 finished! INFO @ Tue, 27 Jun 2017 11:33:22: #2 predicted fragment length is 1 bps INFO @ Tue, 27 Jun 2017 11:33:22: #2 alternative fragment length(s) may be 1,36,576,596 bps INFO @ Tue, 27 Jun 2017 11:33:22: #2.2 Generate R script for model : SRX2761386.05_model.r WARNING @ Tue, 27 Jun 2017 11:33:22: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:33:22: #2 You may need to consider one of the other alternative d(s): 1,36,576,596 WARNING @ Tue, 27 Jun 2017 11:33:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:33:22: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:33:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:33:23: #2 number of paired peaks: 496 WARNING @ Tue, 27 Jun 2017 11:33:23: Fewer paired peaks (496) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 496 pairs to build model! INFO @ Tue, 27 Jun 2017 11:33:23: start model_add_line... INFO @ Tue, 27 Jun 2017 11:33:23: start X-correlation... INFO @ Tue, 27 Jun 2017 11:33:23: end of X-cor INFO @ Tue, 27 Jun 2017 11:33:23: #2 finished! INFO @ Tue, 27 Jun 2017 11:33:23: #2 predicted fragment length is 1 bps INFO @ Tue, 27 Jun 2017 11:33:23: #2 alternative fragment length(s) may be 1,36,576,596 bps INFO @ Tue, 27 Jun 2017 11:33:23: #2.2 Generate R script for model : SRX2761386.20_model.r WARNING @ Tue, 27 Jun 2017 11:33:23: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:33:23: #2 You may need to consider one of the other alternative d(s): 1,36,576,596 WARNING @ Tue, 27 Jun 2017 11:33:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:33:23: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:33:23: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:33:26: 14000000 INFO @ Tue, 27 Jun 2017 11:33:31: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:33:31: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:33:31: #1 total tags in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:33:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:33:31: #1 tags after filtering in treatment: 14618079 INFO @ Tue, 27 Jun 2017 11:33:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:33:31: #1 finished! INFO @ Tue, 27 Jun 2017 11:33:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:33:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:33:32: #2 number of paired peaks: 496 WARNING @ Tue, 27 Jun 2017 11:33:32: Fewer paired peaks (496) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 496 pairs to build model! INFO @ Tue, 27 Jun 2017 11:33:32: start model_add_line... INFO @ Tue, 27 Jun 2017 11:33:32: start X-correlation... INFO @ Tue, 27 Jun 2017 11:33:32: end of X-cor INFO @ Tue, 27 Jun 2017 11:33:32: #2 finished! INFO @ Tue, 27 Jun 2017 11:33:32: #2 predicted fragment length is 1 bps INFO @ Tue, 27 Jun 2017 11:33:32: #2 alternative fragment length(s) may be 1,36,576,596 bps INFO @ Tue, 27 Jun 2017 11:33:32: #2.2 Generate R script for model : SRX2761386.10_model.r WARNING @ Tue, 27 Jun 2017 11:33:32: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:33:32: #2 You may need to consider one of the other alternative d(s): 1,36,576,596 WARNING @ Tue, 27 Jun 2017 11:33:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:33:32: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:33:32: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:33:48: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:33:50: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:34:01: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:34:03: #4 Write output xls file... SRX2761386.05_peaks.xls INFO @ Tue, 27 Jun 2017 11:34:03: #4 Write peak in narrowPeak format file... SRX2761386.05_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:34:03: #4 Write summits bed file... SRX2761386.05_summits.bed INFO @ Tue, 27 Jun 2017 11:34:03: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:34:04: #4 Write output xls file... SRX2761386.20_peaks.xls INFO @ Tue, 27 Jun 2017 11:34:04: #4 Write peak in narrowPeak format file... SRX2761386.20_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:34:04: #4 Write summits bed file... SRX2761386.20_summits.bed INFO @ Tue, 27 Jun 2017 11:34:04: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:34:15: #4 Write output xls file... SRX2761386.10_peaks.xls INFO @ Tue, 27 Jun 2017 11:34:15: #4 Write peak in narrowPeak format file... SRX2761386.10_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:34:15: #4 Write summits bed file... SRX2761386.10_summits.bed INFO @ Tue, 27 Jun 2017 11:34:15: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。