Job ID = 1291774 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T07:30:47 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T07:30:47 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766144' 2019-06-02T07:30:56 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766144' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T07:30:56 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T07:35:46 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T07:35:46 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766145' 2019-06-02T07:35:55 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766145' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T08:15:37 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T08:15:37 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766159' 2019-06-02T08:15:46 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766159' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T08:15:46 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T08:20:38 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T08:20:38 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766160' 2019-06-02T08:20:48 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766160' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T08:20:48 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 2,468,528 reads read : 4,937,056 reads written : 4,937,056 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:03:08 134468528 reads; of these: 134468528 (100.00%) were paired; of these: 75042242 (55.81%) aligned concordantly 0 times 50945630 (37.89%) aligned concordantly exactly 1 time 8480656 (6.31%) aligned concordantly >1 times ---- 75042242 pairs aligned concordantly 0 times; of these: 1950816 (2.60%) aligned discordantly 1 time ---- 73091426 pairs aligned 0 times concordantly or discordantly; of these: 146182852 mates make up the pairs; of these: 143222260 (97.97%) aligned 0 times 1251046 (0.86%) aligned exactly 1 time 1709546 (1.17%) aligned >1 times 46.75% overall alignment rate Time searching: 01:03:08 Overall time: 01:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 29439982 / 61331839 = 0.4800 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 19:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:35:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:35:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:35:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:35:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:35:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:35:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:35:24: 1000000 INFO @ Sun, 02 Jun 2019 19:35:24: 1000000 INFO @ Sun, 02 Jun 2019 19:35:25: 1000000 INFO @ Sun, 02 Jun 2019 19:35:31: 2000000 INFO @ Sun, 02 Jun 2019 19:35:31: 2000000 INFO @ Sun, 02 Jun 2019 19:35:32: 2000000 INFO @ Sun, 02 Jun 2019 19:35:37: 3000000 INFO @ Sun, 02 Jun 2019 19:35:37: 3000000 INFO @ Sun, 02 Jun 2019 19:35:40: 3000000 INFO @ Sun, 02 Jun 2019 19:35:43: 4000000 INFO @ Sun, 02 Jun 2019 19:35:43: 4000000 INFO @ Sun, 02 Jun 2019 19:35:47: 4000000 INFO @ Sun, 02 Jun 2019 19:35:50: 5000000 INFO @ Sun, 02 Jun 2019 19:35:50: 5000000 INFO @ Sun, 02 Jun 2019 19:35:54: 5000000 INFO @ Sun, 02 Jun 2019 19:35:56: 6000000 INFO @ Sun, 02 Jun 2019 19:35:56: 6000000 INFO @ Sun, 02 Jun 2019 19:36:00: 6000000 INFO @ Sun, 02 Jun 2019 19:36:02: 7000000 INFO @ Sun, 02 Jun 2019 19:36:02: 7000000 INFO @ Sun, 02 Jun 2019 19:36:07: 7000000 INFO @ Sun, 02 Jun 2019 19:36:08: 8000000 INFO @ Sun, 02 Jun 2019 19:36:08: 8000000 INFO @ Sun, 02 Jun 2019 19:36:14: 9000000 INFO @ Sun, 02 Jun 2019 19:36:14: 8000000 INFO @ Sun, 02 Jun 2019 19:36:14: 9000000 INFO @ Sun, 02 Jun 2019 19:36:19: 10000000 INFO @ Sun, 02 Jun 2019 19:36:19: 10000000 INFO @ Sun, 02 Jun 2019 19:36:20: 9000000 INFO @ Sun, 02 Jun 2019 19:36:25: 11000000 INFO @ Sun, 02 Jun 2019 19:36:25: 11000000 INFO @ Sun, 02 Jun 2019 19:36:26: 10000000 INFO @ Sun, 02 Jun 2019 19:36:31: 12000000 INFO @ Sun, 02 Jun 2019 19:36:32: 12000000 INFO @ Sun, 02 Jun 2019 19:36:33: 11000000 INFO @ Sun, 02 Jun 2019 19:36:37: 13000000 INFO @ Sun, 02 Jun 2019 19:36:37: 13000000 INFO @ Sun, 02 Jun 2019 19:36:39: 12000000 INFO @ Sun, 02 Jun 2019 19:36:43: 14000000 INFO @ Sun, 02 Jun 2019 19:36:43: 14000000 INFO @ Sun, 02 Jun 2019 19:36:46: 13000000 INFO @ Sun, 02 Jun 2019 19:36:49: 15000000 INFO @ Sun, 02 Jun 2019 19:36:49: 15000000 INFO @ Sun, 02 Jun 2019 19:36:52: 14000000 INFO @ Sun, 02 Jun 2019 19:36:55: 16000000 INFO @ Sun, 02 Jun 2019 19:36:55: 16000000 INFO @ Sun, 02 Jun 2019 19:36:59: 15000000 INFO @ Sun, 02 Jun 2019 19:37:01: 17000000 INFO @ Sun, 02 Jun 2019 19:37:01: 17000000 INFO @ Sun, 02 Jun 2019 19:37:06: 16000000 INFO @ Sun, 02 Jun 2019 19:37:08: 18000000 INFO @ Sun, 02 Jun 2019 19:37:08: 18000000 INFO @ Sun, 02 Jun 2019 19:37:12: 17000000 INFO @ Sun, 02 Jun 2019 19:37:13: 19000000 INFO @ Sun, 02 Jun 2019 19:37:14: 19000000 INFO @ Sun, 02 Jun 2019 19:37:19: 18000000 INFO @ Sun, 02 Jun 2019 19:37:19: 20000000 INFO @ Sun, 02 Jun 2019 19:37:20: 20000000 INFO @ Sun, 02 Jun 2019 19:37:25: 21000000 INFO @ Sun, 02 Jun 2019 19:37:25: 19000000 INFO @ Sun, 02 Jun 2019 19:37:25: 21000000 INFO @ Sun, 02 Jun 2019 19:37:31: 22000000 INFO @ Sun, 02 Jun 2019 19:37:31: 22000000 INFO @ Sun, 02 Jun 2019 19:37:32: 20000000 INFO @ Sun, 02 Jun 2019 19:37:37: 23000000 INFO @ Sun, 02 Jun 2019 19:37:37: 23000000 INFO @ Sun, 02 Jun 2019 19:37:38: 21000000 INFO @ Sun, 02 Jun 2019 19:37:43: 24000000 INFO @ Sun, 02 Jun 2019 19:37:43: 24000000 INFO @ Sun, 02 Jun 2019 19:37:45: 22000000 INFO @ Sun, 02 Jun 2019 19:37:49: 25000000 INFO @ Sun, 02 Jun 2019 19:37:49: 25000000 INFO @ Sun, 02 Jun 2019 19:37:51: 23000000 INFO @ Sun, 02 Jun 2019 19:37:54: 26000000 INFO @ Sun, 02 Jun 2019 19:37:55: 26000000 INFO @ Sun, 02 Jun 2019 19:37:57: 24000000 INFO @ Sun, 02 Jun 2019 19:38:00: 27000000 INFO @ Sun, 02 Jun 2019 19:38:01: 27000000 INFO @ Sun, 02 Jun 2019 19:38:04: 25000000 INFO @ Sun, 02 Jun 2019 19:38:06: 28000000 INFO @ Sun, 02 Jun 2019 19:38:06: 28000000 INFO @ Sun, 02 Jun 2019 19:38:10: 26000000 INFO @ Sun, 02 Jun 2019 19:38:12: 29000000 INFO @ Sun, 02 Jun 2019 19:38:12: 29000000 INFO @ Sun, 02 Jun 2019 19:38:17: 27000000 INFO @ Sun, 02 Jun 2019 19:38:18: 30000000 INFO @ Sun, 02 Jun 2019 19:38:18: 30000000 INFO @ Sun, 02 Jun 2019 19:38:23: 28000000 INFO @ Sun, 02 Jun 2019 19:38:24: 31000000 INFO @ Sun, 02 Jun 2019 19:38:25: 31000000 INFO @ Sun, 02 Jun 2019 19:38:29: 32000000 INFO @ Sun, 02 Jun 2019 19:38:30: 29000000 INFO @ Sun, 02 Jun 2019 19:38:30: 32000000 INFO @ Sun, 02 Jun 2019 19:38:35: 33000000 INFO @ Sun, 02 Jun 2019 19:38:36: 30000000 INFO @ Sun, 02 Jun 2019 19:38:36: 33000000 INFO @ Sun, 02 Jun 2019 19:38:41: 34000000 INFO @ Sun, 02 Jun 2019 19:38:42: 34000000 INFO @ Sun, 02 Jun 2019 19:38:42: 31000000 INFO @ Sun, 02 Jun 2019 19:38:46: 35000000 INFO @ Sun, 02 Jun 2019 19:38:47: 35000000 INFO @ Sun, 02 Jun 2019 19:38:49: 32000000 INFO @ Sun, 02 Jun 2019 19:38:53: 36000000 INFO @ Sun, 02 Jun 2019 19:38:53: 36000000 INFO @ Sun, 02 Jun 2019 19:38:55: 33000000 INFO @ Sun, 02 Jun 2019 19:38:59: 37000000 INFO @ Sun, 02 Jun 2019 19:38:59: 37000000 INFO @ Sun, 02 Jun 2019 19:39:01: 34000000 INFO @ Sun, 02 Jun 2019 19:39:05: 38000000 INFO @ Sun, 02 Jun 2019 19:39:05: 38000000 INFO @ Sun, 02 Jun 2019 19:39:07: 35000000 INFO @ Sun, 02 Jun 2019 19:39:11: 39000000 INFO @ Sun, 02 Jun 2019 19:39:11: 39000000 INFO @ Sun, 02 Jun 2019 19:39:14: 36000000 INFO @ Sun, 02 Jun 2019 19:39:17: 40000000 INFO @ Sun, 02 Jun 2019 19:39:17: 40000000 INFO @ Sun, 02 Jun 2019 19:39:20: 37000000 INFO @ Sun, 02 Jun 2019 19:39:23: 41000000 INFO @ Sun, 02 Jun 2019 19:39:23: 41000000 INFO @ Sun, 02 Jun 2019 19:39:27: 38000000 INFO @ Sun, 02 Jun 2019 19:39:29: 42000000 INFO @ Sun, 02 Jun 2019 19:39:29: 42000000 INFO @ Sun, 02 Jun 2019 19:39:33: 39000000 INFO @ Sun, 02 Jun 2019 19:39:35: 43000000 INFO @ Sun, 02 Jun 2019 19:39:35: 43000000 INFO @ Sun, 02 Jun 2019 19:39:39: 40000000 INFO @ Sun, 02 Jun 2019 19:39:41: 44000000 INFO @ Sun, 02 Jun 2019 19:39:42: 44000000 INFO @ Sun, 02 Jun 2019 19:39:46: 41000000 INFO @ Sun, 02 Jun 2019 19:39:47: 45000000 INFO @ Sun, 02 Jun 2019 19:39:48: 45000000 INFO @ Sun, 02 Jun 2019 19:39:52: 42000000 INFO @ Sun, 02 Jun 2019 19:39:54: 46000000 INFO @ Sun, 02 Jun 2019 19:39:54: 46000000 INFO @ Sun, 02 Jun 2019 19:39:58: 43000000 INFO @ Sun, 02 Jun 2019 19:40:00: 47000000 INFO @ Sun, 02 Jun 2019 19:40:00: 47000000 INFO @ Sun, 02 Jun 2019 19:40:06: 44000000 INFO @ Sun, 02 Jun 2019 19:40:06: 48000000 INFO @ Sun, 02 Jun 2019 19:40:07: 48000000 INFO @ Sun, 02 Jun 2019 19:40:12: 49000000 INFO @ Sun, 02 Jun 2019 19:40:12: 49000000 INFO @ Sun, 02 Jun 2019 19:40:13: 45000000 INFO @ Sun, 02 Jun 2019 19:40:18: 50000000 INFO @ Sun, 02 Jun 2019 19:40:18: 50000000 INFO @ Sun, 02 Jun 2019 19:40:20: 46000000 INFO @ Sun, 02 Jun 2019 19:40:24: 51000000 INFO @ Sun, 02 Jun 2019 19:40:25: 51000000 INFO @ Sun, 02 Jun 2019 19:40:28: 47000000 INFO @ Sun, 02 Jun 2019 19:40:31: 52000000 INFO @ Sun, 02 Jun 2019 19:40:31: 52000000 INFO @ Sun, 02 Jun 2019 19:40:35: 48000000 INFO @ Sun, 02 Jun 2019 19:40:37: 53000000 INFO @ Sun, 02 Jun 2019 19:40:37: 53000000 INFO @ Sun, 02 Jun 2019 19:40:42: 49000000 INFO @ Sun, 02 Jun 2019 19:40:42: 54000000 INFO @ Sun, 02 Jun 2019 19:40:43: 54000000 INFO @ Sun, 02 Jun 2019 19:40:48: 55000000 INFO @ Sun, 02 Jun 2019 19:40:49: 55000000 INFO @ Sun, 02 Jun 2019 19:40:49: 50000000 INFO @ Sun, 02 Jun 2019 19:40:54: 56000000 INFO @ Sun, 02 Jun 2019 19:40:54: 56000000 INFO @ Sun, 02 Jun 2019 19:40:55: 51000000 INFO @ Sun, 02 Jun 2019 19:41:00: 57000000 INFO @ Sun, 02 Jun 2019 19:41:00: 57000000 INFO @ Sun, 02 Jun 2019 19:41:02: 52000000 INFO @ Sun, 02 Jun 2019 19:41:05: 58000000 INFO @ Sun, 02 Jun 2019 19:41:06: 58000000 INFO @ Sun, 02 Jun 2019 19:41:09: 53000000 INFO @ Sun, 02 Jun 2019 19:41:11: 59000000 INFO @ Sun, 02 Jun 2019 19:41:11: 59000000 INFO @ Sun, 02 Jun 2019 19:41:16: 54000000 INFO @ Sun, 02 Jun 2019 19:41:17: 60000000 INFO @ Sun, 02 Jun 2019 19:41:18: 60000000 INFO @ Sun, 02 Jun 2019 19:41:22: 55000000 INFO @ Sun, 02 Jun 2019 19:41:23: 61000000 INFO @ Sun, 02 Jun 2019 19:41:24: 61000000 INFO @ Sun, 02 Jun 2019 19:41:29: 56000000 INFO @ Sun, 02 Jun 2019 19:41:29: 62000000 INFO @ Sun, 02 Jun 2019 19:41:29: 62000000 INFO @ Sun, 02 Jun 2019 19:41:35: 63000000 INFO @ Sun, 02 Jun 2019 19:41:35: 63000000 INFO @ Sun, 02 Jun 2019 19:41:36: 57000000 INFO @ Sun, 02 Jun 2019 19:41:41: 64000000 INFO @ Sun, 02 Jun 2019 19:41:41: 64000000 INFO @ Sun, 02 Jun 2019 19:41:42: 58000000 INFO @ Sun, 02 Jun 2019 19:41:47: 65000000 INFO @ Sun, 02 Jun 2019 19:41:47: 65000000 INFO @ Sun, 02 Jun 2019 19:41:49: 59000000 INFO @ Sun, 02 Jun 2019 19:41:53: 66000000 INFO @ Sun, 02 Jun 2019 19:41:54: 66000000 INFO @ Sun, 02 Jun 2019 19:41:56: 60000000 INFO @ Sun, 02 Jun 2019 19:41:59: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:41:59: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:41:59: #1 total tags in treatment: 30267921 INFO @ Sun, 02 Jun 2019 19:41:59: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:41:59: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:41:59: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:41:59: #1 total tags in treatment: 30267921 INFO @ Sun, 02 Jun 2019 19:41:59: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:42:00: #1 tags after filtering in treatment: 21256846 INFO @ Sun, 02 Jun 2019 19:42:00: #1 Redundant rate of treatment: 0.30 INFO @ Sun, 02 Jun 2019 19:42:00: #1 finished! INFO @ Sun, 02 Jun 2019 19:42:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:42:00: #1 tags after filtering in treatment: 21256846 INFO @ Sun, 02 Jun 2019 19:42:00: #1 Redundant rate of treatment: 0.30 INFO @ Sun, 02 Jun 2019 19:42:00: #1 finished! INFO @ Sun, 02 Jun 2019 19:42:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:42:02: #2 number of paired peaks: 178 WARNING @ Sun, 02 Jun 2019 19:42:02: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sun, 02 Jun 2019 19:42:02: start model_add_line... INFO @ Sun, 02 Jun 2019 19:42:02: start X-correlation... INFO @ Sun, 02 Jun 2019 19:42:02: end of X-cor INFO @ Sun, 02 Jun 2019 19:42:02: #2 finished! INFO @ Sun, 02 Jun 2019 19:42:02: #2 predicted fragment length is 2 bps INFO @ Sun, 02 Jun 2019 19:42:02: #2 alternative fragment length(s) may be 2,29,69,81,109,142,188,261,562,595 bps INFO @ Sun, 02 Jun 2019 19:42:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20_model.r WARNING @ Sun, 02 Jun 2019 19:42:02: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:42:02: #2 You may need to consider one of the other alternative d(s): 2,29,69,81,109,142,188,261,562,595 WARNING @ Sun, 02 Jun 2019 19:42:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:42:02: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:42:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:42:02: #2 number of paired peaks: 178 WARNING @ Sun, 02 Jun 2019 19:42:02: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sun, 02 Jun 2019 19:42:02: start model_add_line... INFO @ Sun, 02 Jun 2019 19:42:02: start X-correlation... INFO @ Sun, 02 Jun 2019 19:42:02: end of X-cor INFO @ Sun, 02 Jun 2019 19:42:02: #2 finished! INFO @ Sun, 02 Jun 2019 19:42:02: #2 predicted fragment length is 2 bps INFO @ Sun, 02 Jun 2019 19:42:02: #2 alternative fragment length(s) may be 2,29,69,81,109,142,188,261,562,595 bps INFO @ Sun, 02 Jun 2019 19:42:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10_model.r WARNING @ Sun, 02 Jun 2019 19:42:02: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:42:02: #2 You may need to consider one of the other alternative d(s): 2,29,69,81,109,142,188,261,562,595 WARNING @ Sun, 02 Jun 2019 19:42:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:42:02: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:42:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:42:02: 61000000 INFO @ Sun, 02 Jun 2019 19:42:09: 62000000 INFO @ Sun, 02 Jun 2019 19:42:15: 63000000 INFO @ Sun, 02 Jun 2019 19:42:21: 64000000 INFO @ Sun, 02 Jun 2019 19:42:27: 65000000 INFO @ Sun, 02 Jun 2019 19:42:33: 66000000 INFO @ Sun, 02 Jun 2019 19:42:39: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:42:39: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:42:39: #1 total tags in treatment: 30267921 INFO @ Sun, 02 Jun 2019 19:42:39: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:42:40: #1 tags after filtering in treatment: 21256846 INFO @ Sun, 02 Jun 2019 19:42:40: #1 Redundant rate of treatment: 0.30 INFO @ Sun, 02 Jun 2019 19:42:40: #1 finished! INFO @ Sun, 02 Jun 2019 19:42:40: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:42:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:42:41: #2 number of paired peaks: 178 WARNING @ Sun, 02 Jun 2019 19:42:41: Fewer paired peaks (178) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 178 pairs to build model! INFO @ Sun, 02 Jun 2019 19:42:41: start model_add_line... INFO @ Sun, 02 Jun 2019 19:42:42: start X-correlation... INFO @ Sun, 02 Jun 2019 19:42:42: end of X-cor INFO @ Sun, 02 Jun 2019 19:42:42: #2 finished! INFO @ Sun, 02 Jun 2019 19:42:42: #2 predicted fragment length is 2 bps INFO @ Sun, 02 Jun 2019 19:42:42: #2 alternative fragment length(s) may be 2,29,69,81,109,142,188,261,562,595 bps INFO @ Sun, 02 Jun 2019 19:42:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05_model.r WARNING @ Sun, 02 Jun 2019 19:42:42: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:42:42: #2 You may need to consider one of the other alternative d(s): 2,29,69,81,109,142,188,261,562,595 WARNING @ Sun, 02 Jun 2019 19:42:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:42:42: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:42:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:42:47: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:42:47: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10_peaks.xls INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.10_summits.bed INFO @ Sun, 02 Jun 2019 19:43:08: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20_peaks.xls INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:43:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.20_summits.bed INFO @ Sun, 02 Jun 2019 19:43:08: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:43:27: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:43:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05_peaks.xls INFO @ Sun, 02 Jun 2019 19:43:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:43:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246027/SRX246027.05_summits.bed INFO @ Sun, 02 Jun 2019 19:43:48: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。