Job ID = 9157217 sra ファイルのダウンロード中... Completed: 491183K bytes transferred in 6 seconds (633882K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 11321125 spots for /home/okishinya/chipatlas/results/ce10/SRX2350725/SRR5024034.sra Written 11321125 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 11321125 reads; of these: 11321125 (100.00%) were unpaired; of these: 605494 (5.35%) aligned 0 times 7978416 (70.47%) aligned exactly 1 time 2737215 (24.18%) aligned >1 times 94.65% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1555383 / 10715631 = 0.1452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:10:35: # Command line: callpeak -t SRX2350725.bam -f BAM -g ce -n SRX2350725.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2350725.10 # format = BAM # ChIP-seq file = ['SRX2350725.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:10:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:10:35: # Command line: callpeak -t SRX2350725.bam -f BAM -g ce -n SRX2350725.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2350725.05 # format = BAM # ChIP-seq file = ['SRX2350725.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:10:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:10:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:10:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:10:35: # Command line: callpeak -t SRX2350725.bam -f BAM -g ce -n SRX2350725.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2350725.20 # format = BAM # ChIP-seq file = ['SRX2350725.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:10:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:10:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:10:44: 1000000 INFO @ Tue, 27 Jun 2017 11:10:44: 1000000 INFO @ Tue, 27 Jun 2017 11:10:44: 1000000 INFO @ Tue, 27 Jun 2017 11:10:51: 2000000 INFO @ Tue, 27 Jun 2017 11:10:51: 2000000 INFO @ Tue, 27 Jun 2017 11:10:51: 2000000 INFO @ Tue, 27 Jun 2017 11:10:59: 3000000 INFO @ Tue, 27 Jun 2017 11:10:59: 3000000 INFO @ Tue, 27 Jun 2017 11:10:59: 3000000 INFO @ Tue, 27 Jun 2017 11:11:06: 4000000 INFO @ Tue, 27 Jun 2017 11:11:07: 4000000 INFO @ Tue, 27 Jun 2017 11:11:07: 4000000 INFO @ Tue, 27 Jun 2017 11:11:15: 5000000 INFO @ Tue, 27 Jun 2017 11:11:15: 5000000 INFO @ Tue, 27 Jun 2017 11:11:15: 5000000 INFO @ Tue, 27 Jun 2017 11:11:23: 6000000 INFO @ Tue, 27 Jun 2017 11:11:24: 6000000 INFO @ Tue, 27 Jun 2017 11:11:24: 6000000 INFO @ Tue, 27 Jun 2017 11:11:31: 7000000 INFO @ Tue, 27 Jun 2017 11:11:31: 7000000 INFO @ Tue, 27 Jun 2017 11:11:31: 7000000 INFO @ Tue, 27 Jun 2017 11:11:39: 8000000 INFO @ Tue, 27 Jun 2017 11:11:39: 8000000 INFO @ Tue, 27 Jun 2017 11:11:39: 8000000 INFO @ Tue, 27 Jun 2017 11:11:46: 9000000 INFO @ Tue, 27 Jun 2017 11:11:46: 9000000 INFO @ Tue, 27 Jun 2017 11:11:46: 9000000 INFO @ Tue, 27 Jun 2017 11:11:47: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:11:47: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:11:47: #1 total tags in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:11:47: #1 tags after filtering in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:11:47: #1 finished! INFO @ Tue, 27 Jun 2017 11:11:47: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:11:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:11:48: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:11:48: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:11:48: #1 total tags in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:11:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:11:48: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:11:48: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:11:48: #1 total tags in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:11:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:11:48: #1 tags after filtering in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:11:48: #1 finished! INFO @ Tue, 27 Jun 2017 11:11:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:11:48: #1 tags after filtering in treatment: 9160248 INFO @ Tue, 27 Jun 2017 11:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:11:48: #1 finished! INFO @ Tue, 27 Jun 2017 11:11:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:11:48: #2 number of paired peaks: 600 WARNING @ Tue, 27 Jun 2017 11:11:48: Fewer paired peaks (600) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 600 pairs to build model! INFO @ Tue, 27 Jun 2017 11:11:48: start model_add_line... INFO @ Tue, 27 Jun 2017 11:11:48: start X-correlation... INFO @ Tue, 27 Jun 2017 11:11:48: end of X-cor INFO @ Tue, 27 Jun 2017 11:11:48: #2 finished! INFO @ Tue, 27 Jun 2017 11:11:48: #2 predicted fragment length is 53 bps INFO @ Tue, 27 Jun 2017 11:11:48: #2 alternative fragment length(s) may be 3,53,569,589,595 bps INFO @ Tue, 27 Jun 2017 11:11:48: #2.2 Generate R script for model : SRX2350725.10_model.r WARNING @ Tue, 27 Jun 2017 11:11:48: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:11:48: #2 You may need to consider one of the other alternative d(s): 3,53,569,589,595 WARNING @ Tue, 27 Jun 2017 11:11:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:11:48: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:11:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:11:48: #2 number of paired peaks: 600 WARNING @ Tue, 27 Jun 2017 11:11:48: Fewer paired peaks (600) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 600 pairs to build model! INFO @ Tue, 27 Jun 2017 11:11:48: start model_add_line... INFO @ Tue, 27 Jun 2017 11:11:48: #2 number of paired peaks: 600 WARNING @ Tue, 27 Jun 2017 11:11:48: Fewer paired peaks (600) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 600 pairs to build model! INFO @ Tue, 27 Jun 2017 11:11:48: start model_add_line... INFO @ Tue, 27 Jun 2017 11:11:49: start X-correlation... INFO @ Tue, 27 Jun 2017 11:11:49: end of X-cor INFO @ Tue, 27 Jun 2017 11:11:49: #2 finished! INFO @ Tue, 27 Jun 2017 11:11:49: #2 predicted fragment length is 53 bps INFO @ Tue, 27 Jun 2017 11:11:49: #2 alternative fragment length(s) may be 3,53,569,589,595 bps INFO @ Tue, 27 Jun 2017 11:11:49: #2.2 Generate R script for model : SRX2350725.05_model.r INFO @ Tue, 27 Jun 2017 11:11:49: start X-correlation... WARNING @ Tue, 27 Jun 2017 11:11:49: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:11:49: #2 You may need to consider one of the other alternative d(s): 3,53,569,589,595 WARNING @ Tue, 27 Jun 2017 11:11:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:11:49: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:11:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:11:49: end of X-cor INFO @ Tue, 27 Jun 2017 11:11:49: #2 finished! INFO @ Tue, 27 Jun 2017 11:11:49: #2 predicted fragment length is 53 bps INFO @ Tue, 27 Jun 2017 11:11:49: #2 alternative fragment length(s) may be 3,53,569,589,595 bps INFO @ Tue, 27 Jun 2017 11:11:49: #2.2 Generate R script for model : SRX2350725.20_model.r WARNING @ Tue, 27 Jun 2017 11:11:49: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 27 Jun 2017 11:11:49: #2 You may need to consider one of the other alternative d(s): 3,53,569,589,595 WARNING @ Tue, 27 Jun 2017 11:11:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 27 Jun 2017 11:11:49: #3 Call peaks... INFO @ Tue, 27 Jun 2017 11:11:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 11:12:08: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:12:09: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:12:10: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 11:12:18: #4 Write output xls file... SRX2350725.20_peaks.xls INFO @ Tue, 27 Jun 2017 11:12:18: #4 Write peak in narrowPeak format file... SRX2350725.20_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:12:18: #4 Write summits bed file... SRX2350725.20_summits.bed INFO @ Tue, 27 Jun 2017 11:12:18: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (198 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:12:19: #4 Write output xls file... SRX2350725.05_peaks.xls INFO @ Tue, 27 Jun 2017 11:12:19: #4 Write peak in narrowPeak format file... SRX2350725.05_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:12:19: #4 Write summits bed file... SRX2350725.05_summits.bed INFO @ Tue, 27 Jun 2017 11:12:19: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (738 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:12:22: #4 Write output xls file... SRX2350725.10_peaks.xls INFO @ Tue, 27 Jun 2017 11:12:22: #4 Write peak in narrowPeak format file... SRX2350725.10_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 11:12:22: #4 Write summits bed file... SRX2350725.10_summits.bed INFO @ Tue, 27 Jun 2017 11:12:22: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (476 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。