Job ID = 2589527 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,019,021 reads read : 6,019,021 reads written : 6,019,021 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR701502.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:54 6019021 reads; of these: 6019021 (100.00%) were unpaired; of these: 1612940 (26.80%) aligned 0 times 3672412 (61.01%) aligned exactly 1 time 733669 (12.19%) aligned >1 times 73.20% overall alignment rate Time searching: 00:00:54 Overall time: 00:00:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 216775 / 4406081 = 0.0492 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:54:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:54:06: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:54:06: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:54:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:54:07: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:54:07: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:54:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:54:08: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:54:08: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:54:14: 1000000 INFO @ Mon, 12 Aug 2019 17:54:17: 1000000 INFO @ Mon, 12 Aug 2019 17:54:20: 1000000 INFO @ Mon, 12 Aug 2019 17:54:21: 2000000 INFO @ Mon, 12 Aug 2019 17:54:25: 2000000 INFO @ Mon, 12 Aug 2019 17:54:29: 3000000 INFO @ Mon, 12 Aug 2019 17:54:30: 2000000 INFO @ Mon, 12 Aug 2019 17:54:34: 3000000 INFO @ Mon, 12 Aug 2019 17:54:36: 4000000 INFO @ Mon, 12 Aug 2019 17:54:37: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:54:37: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:54:37: #1 total tags in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:37: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:54:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:54:38: #1 tags after filtering in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:54:38: #1 finished! INFO @ Mon, 12 Aug 2019 17:54:38: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:54:38: #2 number of paired peaks: 444 WARNING @ Mon, 12 Aug 2019 17:54:38: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Mon, 12 Aug 2019 17:54:38: start model_add_line... INFO @ Mon, 12 Aug 2019 17:54:38: start X-correlation... INFO @ Mon, 12 Aug 2019 17:54:38: end of X-cor INFO @ Mon, 12 Aug 2019 17:54:38: #2 finished! INFO @ Mon, 12 Aug 2019 17:54:38: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 17:54:38: #2 alternative fragment length(s) may be 3,30,564,568,576 bps INFO @ Mon, 12 Aug 2019 17:54:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05_model.r WARNING @ Mon, 12 Aug 2019 17:54:38: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:54:38: #2 You may need to consider one of the other alternative d(s): 3,30,564,568,576 WARNING @ Mon, 12 Aug 2019 17:54:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:54:38: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:54:38: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:54:39: 3000000 INFO @ Mon, 12 Aug 2019 17:54:43: 4000000 INFO @ Mon, 12 Aug 2019 17:54:45: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:54:45: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:54:45: #1 total tags in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:54:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:54:45: #1 tags after filtering in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:54:45: #1 finished! INFO @ Mon, 12 Aug 2019 17:54:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:54:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:54:45: #2 number of paired peaks: 444 WARNING @ Mon, 12 Aug 2019 17:54:45: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Mon, 12 Aug 2019 17:54:45: start model_add_line... INFO @ Mon, 12 Aug 2019 17:54:45: start X-correlation... INFO @ Mon, 12 Aug 2019 17:54:45: end of X-cor INFO @ Mon, 12 Aug 2019 17:54:45: #2 finished! INFO @ Mon, 12 Aug 2019 17:54:45: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 17:54:45: #2 alternative fragment length(s) may be 3,30,564,568,576 bps INFO @ Mon, 12 Aug 2019 17:54:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10_model.r WARNING @ Mon, 12 Aug 2019 17:54:45: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:54:45: #2 You may need to consider one of the other alternative d(s): 3,30,564,568,576 WARNING @ Mon, 12 Aug 2019 17:54:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:54:45: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:54:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:54:47: 4000000 INFO @ Mon, 12 Aug 2019 17:54:49: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:54:49: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:54:49: #1 total tags in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:54:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:54:49: #1 tags after filtering in treatment: 4189306 INFO @ Mon, 12 Aug 2019 17:54:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:54:49: #1 finished! INFO @ Mon, 12 Aug 2019 17:54:49: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:54:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:54:49: #2 number of paired peaks: 444 WARNING @ Mon, 12 Aug 2019 17:54:49: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Mon, 12 Aug 2019 17:54:49: start model_add_line... INFO @ Mon, 12 Aug 2019 17:54:49: start X-correlation... INFO @ Mon, 12 Aug 2019 17:54:49: end of X-cor INFO @ Mon, 12 Aug 2019 17:54:49: #2 finished! INFO @ Mon, 12 Aug 2019 17:54:49: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 17:54:49: #2 alternative fragment length(s) may be 3,30,564,568,576 bps INFO @ Mon, 12 Aug 2019 17:54:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20_model.r WARNING @ Mon, 12 Aug 2019 17:54:49: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:54:49: #2 You may need to consider one of the other alternative d(s): 3,30,564,568,576 WARNING @ Mon, 12 Aug 2019 17:54:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:54:49: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:54:49: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:54:50: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:54:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:54:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:54:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.05_summits.bed INFO @ Mon, 12 Aug 2019 17:54:56: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (455 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:54:57: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:55:01: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:55:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:55:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:55:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.10_summits.bed INFO @ Mon, 12 Aug 2019 17:55:03: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (226 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:55:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:55:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:55:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX233447/SRX233447.20_summits.bed INFO @ Mon, 12 Aug 2019 17:55:07: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (60 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。