Job ID = 10609502 sra ファイルのダウンロード中... Completed: 1183521K bytes transferred in 10 seconds (894494K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 80110862 spots for /home/okishinya/chipatlas/results/ce10/SRX2202789/SRR4319295.sra Written 80110862 spots for /home/okishinya/chipatlas/results/ce10/SRX2202789/SRR4319295.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:17 80110862 reads; of these: 80110862 (100.00%) were unpaired; of these: 2165574 (2.70%) aligned 0 times 64905024 (81.02%) aligned exactly 1 time 13040264 (16.28%) aligned >1 times 97.30% overall alignment rate Time searching: 00:16:17 Overall time: 00:16:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 32404974 / 77945288 = 0.4157 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 07 May 2018 11:20:49: # Command line: callpeak -t SRX2202789.bam -f BAM -g ce -n SRX2202789.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2202789.05 # format = BAM # ChIP-seq file = ['SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 07 May 2018 11:20:49: # Command line: callpeak -t SRX2202789.bam -f BAM -g ce -n SRX2202789.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2202789.20 # format = BAM # ChIP-seq file = ['SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 07 May 2018 11:20:49: #1 read tag files... INFO @ Mon, 07 May 2018 11:20:49: # Command line: callpeak -t SRX2202789.bam -f BAM -g ce -n SRX2202789.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2202789.10 # format = BAM # ChIP-seq file = ['SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 07 May 2018 11:20:49: #1 read tag files... INFO @ Mon, 07 May 2018 11:20:49: #1 read treatment tags... INFO @ Mon, 07 May 2018 11:20:49: #1 read treatment tags... INFO @ Mon, 07 May 2018 11:20:49: #1 read tag files... INFO @ Mon, 07 May 2018 11:20:49: #1 read treatment tags... INFO @ Mon, 07 May 2018 11:20:57: 1000000 INFO @ Mon, 07 May 2018 11:20:57: 1000000 INFO @ Mon, 07 May 2018 11:20:57: 1000000 INFO @ Mon, 07 May 2018 11:21:04: 2000000 INFO @ Mon, 07 May 2018 11:21:04: 2000000 INFO @ Mon, 07 May 2018 11:21:05: 2000000 INFO @ Mon, 07 May 2018 11:21:12: 3000000 INFO @ Mon, 07 May 2018 11:21:12: 3000000 INFO @ Mon, 07 May 2018 11:21:12: 3000000 INFO @ Mon, 07 May 2018 11:21:19: 4000000 INFO @ Mon, 07 May 2018 11:21:20: 4000000 INFO @ Mon, 07 May 2018 11:21:20: 4000000 INFO @ Mon, 07 May 2018 11:21:27: 5000000 INFO @ Mon, 07 May 2018 11:21:28: 5000000 INFO @ Mon, 07 May 2018 11:21:28: 5000000 INFO @ Mon, 07 May 2018 11:21:34: 6000000 INFO @ Mon, 07 May 2018 11:21:35: 6000000 INFO @ Mon, 07 May 2018 11:21:36: 6000000 INFO @ Mon, 07 May 2018 11:21:42: 7000000 INFO @ Mon, 07 May 2018 11:21:43: 7000000 INFO @ Mon, 07 May 2018 11:21:43: 7000000 INFO @ Mon, 07 May 2018 11:21:49: 8000000 INFO @ Mon, 07 May 2018 11:21:51: 8000000 INFO @ Mon, 07 May 2018 11:21:51: 8000000 INFO @ Mon, 07 May 2018 11:21:57: 9000000 INFO @ Mon, 07 May 2018 11:21:59: 9000000 INFO @ Mon, 07 May 2018 11:21:59: 9000000 INFO @ Mon, 07 May 2018 11:22:04: 10000000 INFO @ Mon, 07 May 2018 11:22:07: 10000000 INFO @ Mon, 07 May 2018 11:22:07: 10000000 INFO @ Mon, 07 May 2018 11:22:12: 11000000 INFO @ Mon, 07 May 2018 11:22:15: 11000000 INFO @ Mon, 07 May 2018 11:22:15: 11000000 INFO @ Mon, 07 May 2018 11:22:19: 12000000 INFO @ Mon, 07 May 2018 11:22:22: 12000000 INFO @ Mon, 07 May 2018 11:22:23: 12000000 INFO @ Mon, 07 May 2018 11:22:27: 13000000 INFO @ Mon, 07 May 2018 11:22:30: 13000000 INFO @ Mon, 07 May 2018 11:22:30: 13000000 INFO @ Mon, 07 May 2018 11:22:34: 14000000 INFO @ Mon, 07 May 2018 11:22:38: 14000000 INFO @ Mon, 07 May 2018 11:22:38: 14000000 INFO @ Mon, 07 May 2018 11:22:42: 15000000 INFO @ Mon, 07 May 2018 11:22:46: 15000000 INFO @ Mon, 07 May 2018 11:22:46: 15000000 INFO @ Mon, 07 May 2018 11:22:49: 16000000 INFO @ Mon, 07 May 2018 11:22:54: 16000000 INFO @ Mon, 07 May 2018 11:22:54: 16000000 INFO @ Mon, 07 May 2018 11:22:57: 17000000 INFO @ Mon, 07 May 2018 11:23:01: 17000000 INFO @ Mon, 07 May 2018 11:23:02: 17000000 INFO @ Mon, 07 May 2018 11:23:04: 18000000 INFO @ Mon, 07 May 2018 11:23:09: 18000000 INFO @ Mon, 07 May 2018 11:23:10: 18000000 INFO @ Mon, 07 May 2018 11:23:12: 19000000 INFO @ Mon, 07 May 2018 11:23:17: 19000000 INFO @ Mon, 07 May 2018 11:23:17: 19000000 INFO @ Mon, 07 May 2018 11:23:19: 20000000 INFO @ Mon, 07 May 2018 11:23:25: 20000000 INFO @ Mon, 07 May 2018 11:23:26: 20000000 INFO @ Mon, 07 May 2018 11:23:27: 21000000 INFO @ Mon, 07 May 2018 11:23:33: 21000000 INFO @ Mon, 07 May 2018 11:23:34: 21000000 INFO @ Mon, 07 May 2018 11:23:34: 22000000 INFO @ Mon, 07 May 2018 11:23:42: 23000000 INFO @ Mon, 07 May 2018 11:23:42: 22000000 INFO @ Mon, 07 May 2018 11:23:42: 22000000 INFO @ Mon, 07 May 2018 11:23:49: 24000000 INFO @ Mon, 07 May 2018 11:23:50: 23000000 INFO @ Mon, 07 May 2018 11:23:50: 23000000 INFO @ Mon, 07 May 2018 11:23:56: 25000000 INFO @ Mon, 07 May 2018 11:23:58: 24000000 INFO @ Mon, 07 May 2018 11:23:58: 24000000 INFO @ Mon, 07 May 2018 11:24:04: 26000000 INFO @ Mon, 07 May 2018 11:24:05: 25000000 INFO @ Mon, 07 May 2018 11:24:06: 25000000 INFO @ Mon, 07 May 2018 11:24:12: 27000000 INFO @ Mon, 07 May 2018 11:24:13: 26000000 INFO @ Mon, 07 May 2018 11:24:14: 26000000 INFO @ Mon, 07 May 2018 11:24:20: 28000000 INFO @ Mon, 07 May 2018 11:24:21: 27000000 INFO @ Mon, 07 May 2018 11:24:22: 27000000 INFO @ Mon, 07 May 2018 11:24:28: 28000000 INFO @ Mon, 07 May 2018 11:24:28: 29000000 INFO @ Mon, 07 May 2018 11:24:30: 28000000 INFO @ Mon, 07 May 2018 11:24:36: 29000000 INFO @ Mon, 07 May 2018 11:24:36: 30000000 INFO @ Mon, 07 May 2018 11:24:38: 29000000 INFO @ Mon, 07 May 2018 11:24:44: 30000000 INFO @ Mon, 07 May 2018 11:24:44: 31000000 INFO @ Mon, 07 May 2018 11:24:46: 30000000 INFO @ Mon, 07 May 2018 11:24:51: 31000000 INFO @ Mon, 07 May 2018 11:24:52: 32000000 INFO @ Mon, 07 May 2018 11:24:54: 31000000 INFO @ Mon, 07 May 2018 11:24:59: 32000000 INFO @ Mon, 07 May 2018 11:25:00: 33000000 INFO @ Mon, 07 May 2018 11:25:02: 32000000 INFO @ Mon, 07 May 2018 11:25:06: 33000000 INFO @ Mon, 07 May 2018 11:25:08: 34000000 INFO @ Mon, 07 May 2018 11:25:10: 33000000 INFO @ Mon, 07 May 2018 11:25:14: 34000000 INFO @ Mon, 07 May 2018 11:25:16: 35000000 INFO @ Mon, 07 May 2018 11:25:18: 34000000 INFO @ Mon, 07 May 2018 11:25:22: 35000000 INFO @ Mon, 07 May 2018 11:25:25: 36000000 INFO @ Mon, 07 May 2018 11:25:26: 35000000 INFO @ Mon, 07 May 2018 11:25:29: 36000000 INFO @ Mon, 07 May 2018 11:25:32: 37000000 INFO @ Mon, 07 May 2018 11:25:34: 36000000 INFO @ Mon, 07 May 2018 11:25:37: 37000000 INFO @ Mon, 07 May 2018 11:25:40: 38000000 INFO @ Mon, 07 May 2018 11:25:42: 37000000 INFO @ Mon, 07 May 2018 11:25:44: 38000000 INFO @ Mon, 07 May 2018 11:25:48: 39000000 INFO @ Mon, 07 May 2018 11:25:50: 38000000 INFO @ Mon, 07 May 2018 11:25:52: 39000000 INFO @ Mon, 07 May 2018 11:25:56: 40000000 INFO @ Mon, 07 May 2018 11:25:58: 39000000 INFO @ Mon, 07 May 2018 11:26:00: 40000000 INFO @ Mon, 07 May 2018 11:26:04: 41000000 INFO @ Mon, 07 May 2018 11:26:06: 40000000 INFO @ Mon, 07 May 2018 11:26:07: 41000000 INFO @ Mon, 07 May 2018 11:26:12: 42000000 INFO @ Mon, 07 May 2018 11:26:14: 41000000 INFO @ Mon, 07 May 2018 11:26:15: 42000000 INFO @ Mon, 07 May 2018 11:26:20: 43000000 INFO @ Mon, 07 May 2018 11:26:22: 42000000 INFO @ Mon, 07 May 2018 11:26:23: 43000000 INFO @ Mon, 07 May 2018 11:26:28: 44000000 INFO @ Mon, 07 May 2018 11:26:30: 43000000 INFO @ Mon, 07 May 2018 11:26:30: 44000000 INFO @ Mon, 07 May 2018 11:26:35: 45000000 INFO @ Mon, 07 May 2018 11:26:37: 44000000 INFO @ Mon, 07 May 2018 11:26:38: 45000000 INFO @ Mon, 07 May 2018 11:26:40: #1 tag size is determined as 50 bps INFO @ Mon, 07 May 2018 11:26:40: #1 tag size = 50 INFO @ Mon, 07 May 2018 11:26:40: #1 total tags in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:40: #1 user defined the maximum tags... INFO @ Mon, 07 May 2018 11:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 07 May 2018 11:26:41: #1 tags after filtering in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 07 May 2018 11:26:41: #1 finished! INFO @ Mon, 07 May 2018 11:26:41: #2 Build Peak Model... INFO @ Mon, 07 May 2018 11:26:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 07 May 2018 11:26:42: #1 tag size is determined as 50 bps INFO @ Mon, 07 May 2018 11:26:42: #1 tag size = 50 INFO @ Mon, 07 May 2018 11:26:42: #1 total tags in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:42: #1 user defined the maximum tags... INFO @ Mon, 07 May 2018 11:26:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 07 May 2018 11:26:43: #1 tags after filtering in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 07 May 2018 11:26:43: #1 finished! INFO @ Mon, 07 May 2018 11:26:43: #2 Build Peak Model... INFO @ Mon, 07 May 2018 11:26:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 07 May 2018 11:26:44: #2 number of paired peaks: 0 WARNING @ Mon, 07 May 2018 11:26:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 07 May 2018 11:26:44: Process for pairing-model is terminated! cat: SRX2202789.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2202789.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 07 May 2018 11:26:45: 45000000 INFO @ Mon, 07 May 2018 11:26:46: #2 number of paired peaks: 0 WARNING @ Mon, 07 May 2018 11:26:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 07 May 2018 11:26:46: Process for pairing-model is terminated! cat: SRX2202789.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2202789.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 07 May 2018 11:26:48: #1 tag size is determined as 50 bps INFO @ Mon, 07 May 2018 11:26:48: #1 tag size = 50 INFO @ Mon, 07 May 2018 11:26:48: #1 total tags in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:48: #1 user defined the maximum tags... INFO @ Mon, 07 May 2018 11:26:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 07 May 2018 11:26:49: #1 tags after filtering in treatment: 45540314 INFO @ Mon, 07 May 2018 11:26:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 07 May 2018 11:26:49: #1 finished! INFO @ Mon, 07 May 2018 11:26:49: #2 Build Peak Model... INFO @ Mon, 07 May 2018 11:26:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 07 May 2018 11:26:52: #2 number of paired peaks: 0 WARNING @ Mon, 07 May 2018 11:26:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 07 May 2018 11:26:52: Process for pairing-model is terminated! cat: SRX2202789.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2202789.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2202789.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。