Job ID = 16432753 SRX = SRX15684319 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T00:32:40 prefetch.2.10.7: 1) Downloading 'SRR19633970'... 2022-08-02T00:32:40 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T00:38:37 prefetch.2.10.7: HTTPS download succeed 2022-08-02T00:38:37 prefetch.2.10.7: 1) 'SRR19633970' was downloaded successfully 2022-08-02T00:38:37 prefetch.2.10.7: 'SRR19633970' has 0 unresolved dependencies Read 36888605 spots for SRR19633970/SRR19633970.sra Written 36888605 spots for SRR19633970/SRR19633970.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436580 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:52:25 36888605 reads; of these: 36888605 (100.00%) were paired; of these: 2849387 (7.72%) aligned concordantly 0 times 28344430 (76.84%) aligned concordantly exactly 1 time 5694788 (15.44%) aligned concordantly >1 times ---- 2849387 pairs aligned concordantly 0 times; of these: 483337 (16.96%) aligned discordantly 1 time ---- 2366050 pairs aligned 0 times concordantly or discordantly; of these: 4732100 mates make up the pairs; of these: 3765352 (79.57%) aligned 0 times 524933 (11.09%) aligned exactly 1 time 441815 (9.34%) aligned >1 times 94.90% overall alignment rate Time searching: 01:52:26 Overall time: 01:52:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6643229 / 34455333 = 0.1928 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:19:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:19:43: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:19:43: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:19:53: 1000000 INFO @ Tue, 02 Aug 2022 12:20:02: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:20:11: 3000000 INFO @ Tue, 02 Aug 2022 12:20:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:20:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:20:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:20:21: 4000000 INFO @ Tue, 02 Aug 2022 12:20:23: 1000000 INFO @ Tue, 02 Aug 2022 12:20:30: 5000000 INFO @ Tue, 02 Aug 2022 12:20:33: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:20:40: 6000000 INFO @ Tue, 02 Aug 2022 12:20:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:20:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:20:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:20:43: 3000000 INFO @ Tue, 02 Aug 2022 12:20:50: 7000000 INFO @ Tue, 02 Aug 2022 12:20:54: 1000000 INFO @ Tue, 02 Aug 2022 12:20:55: 4000000 INFO @ Tue, 02 Aug 2022 12:21:01: 8000000 INFO @ Tue, 02 Aug 2022 12:21:05: 2000000 INFO @ Tue, 02 Aug 2022 12:21:07: 5000000 INFO @ Tue, 02 Aug 2022 12:21:10: 9000000 INFO @ Tue, 02 Aug 2022 12:21:17: 3000000 INFO @ Tue, 02 Aug 2022 12:21:18: 6000000 INFO @ Tue, 02 Aug 2022 12:21:20: 10000000 INFO @ Tue, 02 Aug 2022 12:21:28: 4000000 INFO @ Tue, 02 Aug 2022 12:21:30: 7000000 INFO @ Tue, 02 Aug 2022 12:21:31: 11000000 INFO @ Tue, 02 Aug 2022 12:21:41: 5000000 INFO @ Tue, 02 Aug 2022 12:21:41: 8000000 INFO @ Tue, 02 Aug 2022 12:21:43: 12000000 INFO @ Tue, 02 Aug 2022 12:21:52: 9000000 INFO @ Tue, 02 Aug 2022 12:21:53: 6000000 INFO @ Tue, 02 Aug 2022 12:21:54: 13000000 INFO @ Tue, 02 Aug 2022 12:22:03: 10000000 INFO @ Tue, 02 Aug 2022 12:22:04: 14000000 INFO @ Tue, 02 Aug 2022 12:22:04: 7000000 INFO @ Tue, 02 Aug 2022 12:22:13: 15000000 INFO @ Tue, 02 Aug 2022 12:22:14: 11000000 INFO @ Tue, 02 Aug 2022 12:22:16: 8000000 INFO @ Tue, 02 Aug 2022 12:22:22: 16000000 INFO @ Tue, 02 Aug 2022 12:22:25: 12000000 INFO @ Tue, 02 Aug 2022 12:22:27: 9000000 INFO @ Tue, 02 Aug 2022 12:22:32: 17000000 INFO @ Tue, 02 Aug 2022 12:22:37: 13000000 INFO @ Tue, 02 Aug 2022 12:22:37: 10000000 INFO @ Tue, 02 Aug 2022 12:22:42: 18000000 INFO @ Tue, 02 Aug 2022 12:22:48: 11000000 INFO @ Tue, 02 Aug 2022 12:22:49: 14000000 INFO @ Tue, 02 Aug 2022 12:22:51: 19000000 INFO @ Tue, 02 Aug 2022 12:22:59: 15000000 INFO @ Tue, 02 Aug 2022 12:23:00: 12000000 INFO @ Tue, 02 Aug 2022 12:23:01: 20000000 INFO @ Tue, 02 Aug 2022 12:23:10: 16000000 INFO @ Tue, 02 Aug 2022 12:23:10: 21000000 INFO @ Tue, 02 Aug 2022 12:23:12: 13000000 INFO @ Tue, 02 Aug 2022 12:23:20: 22000000 INFO @ Tue, 02 Aug 2022 12:23:20: 17000000 INFO @ Tue, 02 Aug 2022 12:23:24: 14000000 INFO @ Tue, 02 Aug 2022 12:23:29: 23000000 INFO @ Tue, 02 Aug 2022 12:23:31: 18000000 INFO @ Tue, 02 Aug 2022 12:23:35: 15000000 INFO @ Tue, 02 Aug 2022 12:23:39: 24000000 INFO @ Tue, 02 Aug 2022 12:23:42: 19000000 INFO @ Tue, 02 Aug 2022 12:23:45: 16000000 INFO @ Tue, 02 Aug 2022 12:23:48: 25000000 INFO @ Tue, 02 Aug 2022 12:23:53: 20000000 INFO @ Tue, 02 Aug 2022 12:23:56: 17000000 INFO @ Tue, 02 Aug 2022 12:23:57: 26000000 INFO @ Tue, 02 Aug 2022 12:24:04: 21000000 INFO @ Tue, 02 Aug 2022 12:24:07: 27000000 INFO @ Tue, 02 Aug 2022 12:24:07: 18000000 INFO @ Tue, 02 Aug 2022 12:24:15: 22000000 INFO @ Tue, 02 Aug 2022 12:24:16: 28000000 INFO @ Tue, 02 Aug 2022 12:24:18: 19000000 INFO @ Tue, 02 Aug 2022 12:24:26: 29000000 INFO @ Tue, 02 Aug 2022 12:24:26: 23000000 INFO @ Tue, 02 Aug 2022 12:24:29: 20000000 INFO @ Tue, 02 Aug 2022 12:24:35: 30000000 INFO @ Tue, 02 Aug 2022 12:24:38: 24000000 INFO @ Tue, 02 Aug 2022 12:24:40: 21000000 INFO @ Tue, 02 Aug 2022 12:24:44: 31000000 INFO @ Tue, 02 Aug 2022 12:24:48: 25000000 INFO @ Tue, 02 Aug 2022 12:24:51: 22000000 INFO @ Tue, 02 Aug 2022 12:24:54: 32000000 INFO @ Tue, 02 Aug 2022 12:24:59: 26000000 INFO @ Tue, 02 Aug 2022 12:25:02: 23000000 INFO @ Tue, 02 Aug 2022 12:25:03: 33000000 INFO @ Tue, 02 Aug 2022 12:25:10: 27000000 INFO @ Tue, 02 Aug 2022 12:25:13: 24000000 INFO @ Tue, 02 Aug 2022 12:25:13: 34000000 INFO @ Tue, 02 Aug 2022 12:25:21: 28000000 INFO @ Tue, 02 Aug 2022 12:25:23: 35000000 INFO @ Tue, 02 Aug 2022 12:25:24: 25000000 INFO @ Tue, 02 Aug 2022 12:25:32: 29000000 INFO @ Tue, 02 Aug 2022 12:25:32: 36000000 INFO @ Tue, 02 Aug 2022 12:25:35: 26000000 INFO @ Tue, 02 Aug 2022 12:25:42: 37000000 INFO @ Tue, 02 Aug 2022 12:25:43: 30000000 INFO @ Tue, 02 Aug 2022 12:25:46: 27000000 INFO @ Tue, 02 Aug 2022 12:25:52: 38000000 INFO @ Tue, 02 Aug 2022 12:25:54: 31000000 INFO @ Tue, 02 Aug 2022 12:25:57: 28000000 INFO @ Tue, 02 Aug 2022 12:26:01: 39000000 INFO @ Tue, 02 Aug 2022 12:26:05: 32000000 INFO @ Tue, 02 Aug 2022 12:26:08: 29000000 INFO @ Tue, 02 Aug 2022 12:26:11: 40000000 INFO @ Tue, 02 Aug 2022 12:26:16: 33000000 INFO @ Tue, 02 Aug 2022 12:26:18: 30000000 INFO @ Tue, 02 Aug 2022 12:26:20: 41000000 INFO @ Tue, 02 Aug 2022 12:26:28: 34000000 INFO @ Tue, 02 Aug 2022 12:26:30: 31000000 INFO @ Tue, 02 Aug 2022 12:26:30: 42000000 INFO @ Tue, 02 Aug 2022 12:26:38: 35000000 INFO @ Tue, 02 Aug 2022 12:26:39: 43000000 INFO @ Tue, 02 Aug 2022 12:26:40: 32000000 INFO @ Tue, 02 Aug 2022 12:26:48: 44000000 INFO @ Tue, 02 Aug 2022 12:26:49: 36000000 INFO @ Tue, 02 Aug 2022 12:26:51: 33000000 INFO @ Tue, 02 Aug 2022 12:26:57: 45000000 INFO @ Tue, 02 Aug 2022 12:27:00: 37000000 INFO @ Tue, 02 Aug 2022 12:27:03: 34000000 INFO @ Tue, 02 Aug 2022 12:27:06: 46000000 INFO @ Tue, 02 Aug 2022 12:27:12: 38000000 INFO @ Tue, 02 Aug 2022 12:27:15: 35000000 INFO @ Tue, 02 Aug 2022 12:27:17: 47000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:27:23: 39000000 INFO @ Tue, 02 Aug 2022 12:27:26: 36000000 INFO @ Tue, 02 Aug 2022 12:27:26: 48000000 INFO @ Tue, 02 Aug 2022 12:27:34: 40000000 INFO @ Tue, 02 Aug 2022 12:27:35: 49000000 INFO @ Tue, 02 Aug 2022 12:27:37: 37000000 INFO @ Tue, 02 Aug 2022 12:27:44: 50000000 INFO @ Tue, 02 Aug 2022 12:27:46: 41000000 INFO @ Tue, 02 Aug 2022 12:27:48: 38000000 INFO @ Tue, 02 Aug 2022 12:27:53: 51000000 INFO @ Tue, 02 Aug 2022 12:27:57: 42000000 INFO @ Tue, 02 Aug 2022 12:27:59: 39000000 INFO @ Tue, 02 Aug 2022 12:28:02: 52000000 INFO @ Tue, 02 Aug 2022 12:28:08: 43000000 INFO @ Tue, 02 Aug 2022 12:28:09: 40000000 INFO @ Tue, 02 Aug 2022 12:28:11: 53000000 INFO @ Tue, 02 Aug 2022 12:28:19: 44000000 INFO @ Tue, 02 Aug 2022 12:28:20: 41000000 INFO @ Tue, 02 Aug 2022 12:28:20: 54000000 INFO @ Tue, 02 Aug 2022 12:28:29: 55000000 INFO @ Tue, 02 Aug 2022 12:28:31: 42000000 INFO @ Tue, 02 Aug 2022 12:28:31: 45000000 INFO @ Tue, 02 Aug 2022 12:28:38: 56000000 INFO @ Tue, 02 Aug 2022 12:28:42: 43000000 INFO @ Tue, 02 Aug 2022 12:28:43: 46000000 INFO @ Tue, 02 Aug 2022 12:28:46: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:28:46: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:28:46: #1 total tags in treatment: 27452945 INFO @ Tue, 02 Aug 2022 12:28:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:28:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:28:46: #1 tags after filtering in treatment: 23873193 INFO @ Tue, 02 Aug 2022 12:28:46: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 02 Aug 2022 12:28:46: #1 finished! INFO @ Tue, 02 Aug 2022 12:28:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:28:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:28:48: #2 number of paired peaks: 240 WARNING @ Tue, 02 Aug 2022 12:28:48: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Tue, 02 Aug 2022 12:28:48: start model_add_line... INFO @ Tue, 02 Aug 2022 12:28:48: start X-correlation... INFO @ Tue, 02 Aug 2022 12:28:48: end of X-cor INFO @ Tue, 02 Aug 2022 12:28:48: #2 finished! INFO @ Tue, 02 Aug 2022 12:28:48: #2 predicted fragment length is 173 bps INFO @ Tue, 02 Aug 2022 12:28:48: #2 alternative fragment length(s) may be 3,173 bps INFO @ Tue, 02 Aug 2022 12:28:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05_model.r WARNING @ Tue, 02 Aug 2022 12:28:48: #2 Since the d (173) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:28:48: #2 You may need to consider one of the other alternative d(s): 3,173 WARNING @ Tue, 02 Aug 2022 12:28:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:28:48: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:28:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:28:53: 44000000 INFO @ Tue, 02 Aug 2022 12:28:55: 47000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:29:04: 45000000 INFO @ Tue, 02 Aug 2022 12:29:08: 48000000 INFO @ Tue, 02 Aug 2022 12:29:16: 46000000 INFO @ Tue, 02 Aug 2022 12:29:20: 49000000 INFO @ Tue, 02 Aug 2022 12:29:28: 47000000 INFO @ Tue, 02 Aug 2022 12:29:32: 50000000 INFO @ Tue, 02 Aug 2022 12:29:32: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:29:40: 48000000 INFO @ Tue, 02 Aug 2022 12:29:44: 51000000 INFO @ Tue, 02 Aug 2022 12:29:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:29:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:29:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.05_summits.bed INFO @ Tue, 02 Aug 2022 12:29:52: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1043 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:29:52: 49000000 INFO @ Tue, 02 Aug 2022 12:29:57: 52000000 INFO @ Tue, 02 Aug 2022 12:30:04: 50000000 INFO @ Tue, 02 Aug 2022 12:30:09: 53000000 INFO @ Tue, 02 Aug 2022 12:30:16: 51000000 INFO @ Tue, 02 Aug 2022 12:30:21: 54000000 INFO @ Tue, 02 Aug 2022 12:30:27: 52000000 INFO @ Tue, 02 Aug 2022 12:30:32: 55000000 INFO @ Tue, 02 Aug 2022 12:30:39: 53000000 INFO @ Tue, 02 Aug 2022 12:30:44: 56000000 INFO @ Tue, 02 Aug 2022 12:30:51: 54000000 INFO @ Tue, 02 Aug 2022 12:30:52: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:30:52: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:30:52: #1 total tags in treatment: 27452945 INFO @ Tue, 02 Aug 2022 12:30:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:30:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:30:53: #1 tags after filtering in treatment: 23873193 INFO @ Tue, 02 Aug 2022 12:30:53: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 02 Aug 2022 12:30:53: #1 finished! INFO @ Tue, 02 Aug 2022 12:30:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:30:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:30:54: #2 number of paired peaks: 240 WARNING @ Tue, 02 Aug 2022 12:30:54: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Tue, 02 Aug 2022 12:30:54: start model_add_line... INFO @ Tue, 02 Aug 2022 12:30:54: start X-correlation... INFO @ Tue, 02 Aug 2022 12:30:54: end of X-cor INFO @ Tue, 02 Aug 2022 12:30:54: #2 finished! INFO @ Tue, 02 Aug 2022 12:30:54: #2 predicted fragment length is 173 bps INFO @ Tue, 02 Aug 2022 12:30:54: #2 alternative fragment length(s) may be 3,173 bps INFO @ Tue, 02 Aug 2022 12:30:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10_model.r WARNING @ Tue, 02 Aug 2022 12:30:54: #2 Since the d (173) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:30:54: #2 You may need to consider one of the other alternative d(s): 3,173 WARNING @ Tue, 02 Aug 2022 12:30:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:30:54: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:30:54: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:31:01: 55000000 INFO @ Tue, 02 Aug 2022 12:31:10: 56000000 INFO @ Tue, 02 Aug 2022 12:31:17: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:31:17: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:31:17: #1 total tags in treatment: 27452945 INFO @ Tue, 02 Aug 2022 12:31:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:31:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:31:18: #1 tags after filtering in treatment: 23873193 INFO @ Tue, 02 Aug 2022 12:31:18: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 02 Aug 2022 12:31:18: #1 finished! INFO @ Tue, 02 Aug 2022 12:31:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:31:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:31:19: #2 number of paired peaks: 240 WARNING @ Tue, 02 Aug 2022 12:31:19: Fewer paired peaks (240) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 240 pairs to build model! INFO @ Tue, 02 Aug 2022 12:31:19: start model_add_line... INFO @ Tue, 02 Aug 2022 12:31:19: start X-correlation... INFO @ Tue, 02 Aug 2022 12:31:19: end of X-cor INFO @ Tue, 02 Aug 2022 12:31:19: #2 finished! INFO @ Tue, 02 Aug 2022 12:31:19: #2 predicted fragment length is 173 bps INFO @ Tue, 02 Aug 2022 12:31:19: #2 alternative fragment length(s) may be 3,173 bps INFO @ Tue, 02 Aug 2022 12:31:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20_model.r WARNING @ Tue, 02 Aug 2022 12:31:19: #2 Since the d (173) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:31:19: #2 You may need to consider one of the other alternative d(s): 3,173 WARNING @ Tue, 02 Aug 2022 12:31:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:31:19: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:31:19: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:31:39: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:31:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:31:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:31:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.10_summits.bed INFO @ Tue, 02 Aug 2022 12:31:58: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (523 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:32:03: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:32:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:32:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:32:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684319/SRX15684319.20_summits.bed INFO @ Tue, 02 Aug 2022 12:32:22: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (324 records, 4 fields): 55 millis CompletedMACS2peakCalling