Job ID = 16432718 SRX = SRX15684317 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T00:32:38 prefetch.2.10.7: 1) Downloading 'SRR19633972'... 2022-08-02T00:32:38 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T00:40:36 prefetch.2.10.7: HTTPS download succeed 2022-08-02T00:40:36 prefetch.2.10.7: 1) 'SRR19633972' was downloaded successfully 2022-08-02T00:40:36 prefetch.2.10.7: 'SRR19633972' has 0 unresolved dependencies Read 54297396 spots for SRR19633972/SRR19633972.sra Written 54297396 spots for SRR19633972/SRR19633972.sra fastq に変換しました。 bowtie でマッピング中... Your job 16437581 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:52:16 54297396 reads; of these: 54297396 (100.00%) were paired; of these: 7763486 (14.30%) aligned concordantly 0 times 36920406 (68.00%) aligned concordantly exactly 1 time 9613504 (17.71%) aligned concordantly >1 times ---- 7763486 pairs aligned concordantly 0 times; of these: 1403430 (18.08%) aligned discordantly 1 time ---- 6360056 pairs aligned 0 times concordantly or discordantly; of these: 12720112 mates make up the pairs; of these: 10984004 (86.35%) aligned 0 times 850334 (6.68%) aligned exactly 1 time 885774 (6.96%) aligned >1 times 89.89% overall alignment rate Time searching: 02:52:17 Overall time: 02:52:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 72 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 11439303 / 47780553 = 0.2394 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:36:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:36:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:36:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:36:16: 1000000 INFO @ Tue, 02 Aug 2022 13:36:25: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:36:36: 3000000 INFO @ Tue, 02 Aug 2022 13:36:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:36:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:36:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:36:47: 4000000 INFO @ Tue, 02 Aug 2022 13:36:47: 1000000 INFO @ Tue, 02 Aug 2022 13:36:58: 5000000 INFO @ Tue, 02 Aug 2022 13:36:58: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:37:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:37:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:37:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:37:10: 3000000 INFO @ Tue, 02 Aug 2022 13:37:11: 6000000 INFO @ Tue, 02 Aug 2022 13:37:18: 1000000 INFO @ Tue, 02 Aug 2022 13:37:22: 4000000 INFO @ Tue, 02 Aug 2022 13:37:24: 7000000 INFO @ Tue, 02 Aug 2022 13:37:30: 2000000 INFO @ Tue, 02 Aug 2022 13:37:33: 5000000 INFO @ Tue, 02 Aug 2022 13:37:38: 8000000 INFO @ Tue, 02 Aug 2022 13:37:42: 3000000 INFO @ Tue, 02 Aug 2022 13:37:45: 6000000 INFO @ Tue, 02 Aug 2022 13:37:51: 9000000 INFO @ Tue, 02 Aug 2022 13:37:54: 4000000 INFO @ Tue, 02 Aug 2022 13:37:56: 7000000 INFO @ Tue, 02 Aug 2022 13:38:02: 10000000 INFO @ Tue, 02 Aug 2022 13:38:06: 5000000 INFO @ Tue, 02 Aug 2022 13:38:08: 8000000 INFO @ Tue, 02 Aug 2022 13:38:14: 11000000 INFO @ Tue, 02 Aug 2022 13:38:17: 6000000 INFO @ Tue, 02 Aug 2022 13:38:20: 9000000 INFO @ Tue, 02 Aug 2022 13:38:26: 12000000 INFO @ Tue, 02 Aug 2022 13:38:29: 7000000 INFO @ Tue, 02 Aug 2022 13:38:32: 10000000 INFO @ Tue, 02 Aug 2022 13:38:37: 13000000 INFO @ Tue, 02 Aug 2022 13:38:41: 8000000 INFO @ Tue, 02 Aug 2022 13:38:44: 11000000 INFO @ Tue, 02 Aug 2022 13:38:49: 14000000 INFO @ Tue, 02 Aug 2022 13:38:53: 9000000 INFO @ Tue, 02 Aug 2022 13:38:56: 12000000 INFO @ Tue, 02 Aug 2022 13:39:01: 15000000 INFO @ Tue, 02 Aug 2022 13:39:05: 10000000 INFO @ Tue, 02 Aug 2022 13:39:08: 13000000 INFO @ Tue, 02 Aug 2022 13:39:12: 16000000 INFO @ Tue, 02 Aug 2022 13:39:17: 11000000 INFO @ Tue, 02 Aug 2022 13:39:20: 14000000 INFO @ Tue, 02 Aug 2022 13:39:24: 17000000 INFO @ Tue, 02 Aug 2022 13:39:28: 12000000 INFO @ Tue, 02 Aug 2022 13:39:33: 15000000 INFO @ Tue, 02 Aug 2022 13:39:36: 18000000 INFO @ Tue, 02 Aug 2022 13:39:40: 13000000 INFO @ Tue, 02 Aug 2022 13:39:44: 16000000 INFO @ Tue, 02 Aug 2022 13:39:47: 19000000 INFO @ Tue, 02 Aug 2022 13:39:51: 14000000 INFO @ Tue, 02 Aug 2022 13:39:57: 17000000 INFO @ Tue, 02 Aug 2022 13:39:58: 20000000 INFO @ Tue, 02 Aug 2022 13:40:03: 15000000 INFO @ Tue, 02 Aug 2022 13:40:08: 18000000 INFO @ Tue, 02 Aug 2022 13:40:08: 21000000 INFO @ Tue, 02 Aug 2022 13:40:15: 16000000 INFO @ Tue, 02 Aug 2022 13:40:19: 22000000 INFO @ Tue, 02 Aug 2022 13:40:20: 19000000 INFO @ Tue, 02 Aug 2022 13:40:27: 17000000 INFO @ Tue, 02 Aug 2022 13:40:29: 23000000 INFO @ Tue, 02 Aug 2022 13:40:33: 20000000 INFO @ Tue, 02 Aug 2022 13:40:39: 18000000 INFO @ Tue, 02 Aug 2022 13:40:40: 24000000 INFO @ Tue, 02 Aug 2022 13:40:45: 21000000 INFO @ Tue, 02 Aug 2022 13:40:50: 25000000 INFO @ Tue, 02 Aug 2022 13:40:52: 19000000 INFO @ Tue, 02 Aug 2022 13:40:57: 22000000 INFO @ Tue, 02 Aug 2022 13:41:00: 26000000 INFO @ Tue, 02 Aug 2022 13:41:05: 20000000 INFO @ Tue, 02 Aug 2022 13:41:10: 23000000 INFO @ Tue, 02 Aug 2022 13:41:11: 27000000 INFO @ Tue, 02 Aug 2022 13:41:17: 21000000 INFO @ Tue, 02 Aug 2022 13:41:21: 28000000 INFO @ Tue, 02 Aug 2022 13:41:21: 24000000 INFO @ Tue, 02 Aug 2022 13:41:29: 22000000 INFO @ Tue, 02 Aug 2022 13:41:31: 29000000 INFO @ Tue, 02 Aug 2022 13:41:33: 25000000 INFO @ Tue, 02 Aug 2022 13:41:41: 30000000 INFO @ Tue, 02 Aug 2022 13:41:42: 23000000 INFO @ Tue, 02 Aug 2022 13:41:45: 26000000 INFO @ Tue, 02 Aug 2022 13:41:49: 31000000 INFO @ Tue, 02 Aug 2022 13:41:54: 24000000 INFO @ Tue, 02 Aug 2022 13:41:57: 27000000 INFO @ Tue, 02 Aug 2022 13:41:57: 32000000 INFO @ Tue, 02 Aug 2022 13:42:06: 25000000 INFO @ Tue, 02 Aug 2022 13:42:06: 33000000 INFO @ Tue, 02 Aug 2022 13:42:08: 28000000 INFO @ Tue, 02 Aug 2022 13:42:15: 34000000 INFO @ Tue, 02 Aug 2022 13:42:18: 26000000 INFO @ Tue, 02 Aug 2022 13:42:20: 29000000 INFO @ Tue, 02 Aug 2022 13:42:24: 35000000 INFO @ Tue, 02 Aug 2022 13:42:30: 27000000 INFO @ Tue, 02 Aug 2022 13:42:33: 30000000 INFO @ Tue, 02 Aug 2022 13:42:33: 36000000 INFO @ Tue, 02 Aug 2022 13:42:42: 37000000 INFO @ Tue, 02 Aug 2022 13:42:42: 28000000 INFO @ Tue, 02 Aug 2022 13:42:45: 31000000 INFO @ Tue, 02 Aug 2022 13:42:50: 38000000 INFO @ Tue, 02 Aug 2022 13:42:54: 29000000 INFO @ Tue, 02 Aug 2022 13:42:57: 32000000 INFO @ Tue, 02 Aug 2022 13:42:58: 39000000 INFO @ Tue, 02 Aug 2022 13:43:05: 30000000 INFO @ Tue, 02 Aug 2022 13:43:07: 40000000 INFO @ Tue, 02 Aug 2022 13:43:08: 33000000 INFO @ Tue, 02 Aug 2022 13:43:16: 41000000 INFO @ Tue, 02 Aug 2022 13:43:17: 31000000 INFO @ Tue, 02 Aug 2022 13:43:20: 34000000 INFO @ Tue, 02 Aug 2022 13:43:25: 42000000 INFO @ Tue, 02 Aug 2022 13:43:29: 32000000 INFO @ Tue, 02 Aug 2022 13:43:32: 35000000 INFO @ Tue, 02 Aug 2022 13:43:33: 43000000 INFO @ Tue, 02 Aug 2022 13:43:41: 33000000 INFO @ Tue, 02 Aug 2022 13:43:42: 44000000 INFO @ Tue, 02 Aug 2022 13:43:44: 36000000 INFO @ Tue, 02 Aug 2022 13:43:51: 45000000 INFO @ Tue, 02 Aug 2022 13:43:53: 34000000 INFO @ Tue, 02 Aug 2022 13:43:56: 37000000 INFO @ Tue, 02 Aug 2022 13:43:59: 46000000 INFO @ Tue, 02 Aug 2022 13:44:03: 35000000 INFO @ Tue, 02 Aug 2022 13:44:07: 47000000 INFO @ Tue, 02 Aug 2022 13:44:08: 38000000 INFO @ Tue, 02 Aug 2022 13:44:15: 36000000 INFO @ Tue, 02 Aug 2022 13:44:16: 48000000 INFO @ Tue, 02 Aug 2022 13:44:21: 39000000 INFO @ Tue, 02 Aug 2022 13:44:24: 49000000 INFO @ Tue, 02 Aug 2022 13:44:27: 37000000 INFO @ Tue, 02 Aug 2022 13:44:33: 40000000 INFO @ Tue, 02 Aug 2022 13:44:33: 50000000 INFO @ Tue, 02 Aug 2022 13:44:39: 38000000 INFO @ Tue, 02 Aug 2022 13:44:41: 51000000 INFO @ Tue, 02 Aug 2022 13:44:45: 41000000 INFO @ Tue, 02 Aug 2022 13:44:49: 52000000 INFO @ Tue, 02 Aug 2022 13:44:51: 39000000 INFO @ Tue, 02 Aug 2022 13:44:57: 42000000 INFO @ Tue, 02 Aug 2022 13:44:58: 53000000 INFO @ Tue, 02 Aug 2022 13:45:03: 40000000 INFO @ Tue, 02 Aug 2022 13:45:06: 54000000 INFO @ Tue, 02 Aug 2022 13:45:09: 43000000 INFO @ Tue, 02 Aug 2022 13:45:14: 55000000 INFO @ Tue, 02 Aug 2022 13:45:15: 41000000 INFO @ Tue, 02 Aug 2022 13:45:21: 44000000 INFO @ Tue, 02 Aug 2022 13:45:24: 56000000 INFO @ Tue, 02 Aug 2022 13:45:27: 42000000 INFO @ Tue, 02 Aug 2022 13:45:32: 45000000 INFO @ Tue, 02 Aug 2022 13:45:34: 57000000 INFO @ Tue, 02 Aug 2022 13:45:40: 43000000 INFO @ Tue, 02 Aug 2022 13:45:43: 58000000 INFO @ Tue, 02 Aug 2022 13:45:44: 46000000 INFO @ Tue, 02 Aug 2022 13:45:49: 44000000 INFO @ Tue, 02 Aug 2022 13:45:52: 59000000 INFO @ Tue, 02 Aug 2022 13:45:56: 47000000 INFO @ Tue, 02 Aug 2022 13:46:01: 45000000 INFO @ Tue, 02 Aug 2022 13:46:03: 60000000 INFO @ Tue, 02 Aug 2022 13:46:09: 48000000 INFO @ Tue, 02 Aug 2022 13:46:12: 61000000 INFO @ Tue, 02 Aug 2022 13:46:13: 46000000 INFO @ Tue, 02 Aug 2022 13:46:21: 62000000 INFO @ Tue, 02 Aug 2022 13:46:21: 49000000 INFO @ Tue, 02 Aug 2022 13:46:25: 47000000 INFO @ Tue, 02 Aug 2022 13:46:30: 63000000 INFO @ Tue, 02 Aug 2022 13:46:34: 50000000 INFO @ Tue, 02 Aug 2022 13:46:37: 48000000 INFO @ Tue, 02 Aug 2022 13:46:38: 64000000 INFO @ Tue, 02 Aug 2022 13:46:46: 51000000 INFO @ Tue, 02 Aug 2022 13:46:46: 65000000 INFO @ Tue, 02 Aug 2022 13:46:49: 49000000 INFO @ Tue, 02 Aug 2022 13:46:55: 66000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:46:59: 52000000 INFO @ Tue, 02 Aug 2022 13:47:01: 50000000 INFO @ Tue, 02 Aug 2022 13:47:03: 67000000 INFO @ Tue, 02 Aug 2022 13:47:11: 53000000 INFO @ Tue, 02 Aug 2022 13:47:12: 68000000 INFO @ Tue, 02 Aug 2022 13:47:13: 51000000 INFO @ Tue, 02 Aug 2022 13:47:20: 69000000 INFO @ Tue, 02 Aug 2022 13:47:23: 54000000 INFO @ Tue, 02 Aug 2022 13:47:25: 52000000 INFO @ Tue, 02 Aug 2022 13:47:29: 70000000 INFO @ Tue, 02 Aug 2022 13:47:36: 55000000 INFO @ Tue, 02 Aug 2022 13:47:37: 71000000 INFO @ Tue, 02 Aug 2022 13:47:37: 53000000 INFO @ Tue, 02 Aug 2022 13:47:45: 72000000 INFO @ Tue, 02 Aug 2022 13:47:48: 56000000 INFO @ Tue, 02 Aug 2022 13:47:49: 54000000 INFO @ Tue, 02 Aug 2022 13:47:54: 73000000 INFO @ Tue, 02 Aug 2022 13:48:00: 57000000 INFO @ Tue, 02 Aug 2022 13:48:01: 55000000 INFO @ Tue, 02 Aug 2022 13:48:02: 74000000 INFO @ Tue, 02 Aug 2022 13:48:09: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:48:09: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:48:09: #1 total tags in treatment: 35284953 INFO @ Tue, 02 Aug 2022 13:48:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:48:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:48:10: #1 tags after filtering in treatment: 28970100 INFO @ Tue, 02 Aug 2022 13:48:10: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 02 Aug 2022 13:48:10: #1 finished! INFO @ Tue, 02 Aug 2022 13:48:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:48:12: #2 number of paired peaks: 191 WARNING @ Tue, 02 Aug 2022 13:48:12: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Tue, 02 Aug 2022 13:48:12: start model_add_line... INFO @ Tue, 02 Aug 2022 13:48:12: 58000000 INFO @ Tue, 02 Aug 2022 13:48:12: start X-correlation... INFO @ Tue, 02 Aug 2022 13:48:12: end of X-cor INFO @ Tue, 02 Aug 2022 13:48:12: #2 finished! INFO @ Tue, 02 Aug 2022 13:48:12: #2 predicted fragment length is 142 bps INFO @ Tue, 02 Aug 2022 13:48:12: #2 alternative fragment length(s) may be 2,142 bps INFO @ Tue, 02 Aug 2022 13:48:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05_model.r WARNING @ Tue, 02 Aug 2022 13:48:12: #2 Since the d (142) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:48:12: #2 You may need to consider one of the other alternative d(s): 2,142 WARNING @ Tue, 02 Aug 2022 13:48:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:48:12: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:48:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:48:12: 56000000 INFO @ Tue, 02 Aug 2022 13:48:24: 59000000 INFO @ Tue, 02 Aug 2022 13:48:24: 57000000 INFO @ Tue, 02 Aug 2022 13:48:36: 60000000 INFO @ Tue, 02 Aug 2022 13:48:36: 58000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 13:48:47: 61000000 INFO @ Tue, 02 Aug 2022 13:48:48: 59000000 INFO @ Tue, 02 Aug 2022 13:48:59: 62000000 INFO @ Tue, 02 Aug 2022 13:48:59: 60000000 INFO @ Tue, 02 Aug 2022 13:49:03: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:49:11: 63000000 INFO @ Tue, 02 Aug 2022 13:49:11: 61000000 INFO @ Tue, 02 Aug 2022 13:49:22: 64000000 INFO @ Tue, 02 Aug 2022 13:49:23: 62000000 INFO @ Tue, 02 Aug 2022 13:49:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05_peaks.xls INFO @ Tue, 02 Aug 2022 13:49:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:49:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.05_summits.bed INFO @ Tue, 02 Aug 2022 13:49:25: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2472 records, 4 fields): 35 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:49:34: 65000000 INFO @ Tue, 02 Aug 2022 13:49:35: 63000000 INFO @ Tue, 02 Aug 2022 13:49:46: 66000000 INFO @ Tue, 02 Aug 2022 13:49:46: 64000000 INFO @ Tue, 02 Aug 2022 13:49:57: 67000000 INFO @ Tue, 02 Aug 2022 13:49:58: 65000000 INFO @ Tue, 02 Aug 2022 13:50:09: 68000000 INFO @ Tue, 02 Aug 2022 13:50:09: 66000000 INFO @ Tue, 02 Aug 2022 13:50:21: 69000000 INFO @ Tue, 02 Aug 2022 13:50:22: 67000000 INFO @ Tue, 02 Aug 2022 13:50:32: 70000000 INFO @ Tue, 02 Aug 2022 13:50:34: 68000000 INFO @ Tue, 02 Aug 2022 13:50:44: 71000000 INFO @ Tue, 02 Aug 2022 13:50:46: 69000000 INFO @ Tue, 02 Aug 2022 13:50:56: 72000000 INFO @ Tue, 02 Aug 2022 13:50:57: 70000000 INFO @ Tue, 02 Aug 2022 13:51:07: 73000000 INFO @ Tue, 02 Aug 2022 13:51:09: 71000000 INFO @ Tue, 02 Aug 2022 13:51:19: 74000000 INFO @ Tue, 02 Aug 2022 13:51:21: 72000000 INFO @ Tue, 02 Aug 2022 13:51:28: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:51:28: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:51:28: #1 total tags in treatment: 35284953 INFO @ Tue, 02 Aug 2022 13:51:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:51:29: #1 tags after filtering in treatment: 28970100 INFO @ Tue, 02 Aug 2022 13:51:29: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 02 Aug 2022 13:51:29: #1 finished! INFO @ Tue, 02 Aug 2022 13:51:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:51:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:51:31: #2 number of paired peaks: 191 WARNING @ Tue, 02 Aug 2022 13:51:31: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Tue, 02 Aug 2022 13:51:31: start model_add_line... INFO @ Tue, 02 Aug 2022 13:51:31: start X-correlation... INFO @ Tue, 02 Aug 2022 13:51:31: end of X-cor INFO @ Tue, 02 Aug 2022 13:51:31: #2 finished! INFO @ Tue, 02 Aug 2022 13:51:31: #2 predicted fragment length is 142 bps INFO @ Tue, 02 Aug 2022 13:51:31: #2 alternative fragment length(s) may be 2,142 bps INFO @ Tue, 02 Aug 2022 13:51:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10_model.r WARNING @ Tue, 02 Aug 2022 13:51:31: #2 Since the d (142) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:51:31: #2 You may need to consider one of the other alternative d(s): 2,142 WARNING @ Tue, 02 Aug 2022 13:51:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:51:31: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:51:31: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:51:32: 73000000 INFO @ Tue, 02 Aug 2022 13:51:42: 74000000 INFO @ Tue, 02 Aug 2022 13:51:49: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:51:49: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:51:49: #1 total tags in treatment: 35284953 INFO @ Tue, 02 Aug 2022 13:51:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:51:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:51:50: #1 tags after filtering in treatment: 28970100 INFO @ Tue, 02 Aug 2022 13:51:50: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 02 Aug 2022 13:51:50: #1 finished! INFO @ Tue, 02 Aug 2022 13:51:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:51:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:51:52: #2 number of paired peaks: 191 WARNING @ Tue, 02 Aug 2022 13:51:52: Fewer paired peaks (191) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 191 pairs to build model! INFO @ Tue, 02 Aug 2022 13:51:52: start model_add_line... INFO @ Tue, 02 Aug 2022 13:51:52: start X-correlation... INFO @ Tue, 02 Aug 2022 13:51:52: end of X-cor INFO @ Tue, 02 Aug 2022 13:51:52: #2 finished! INFO @ Tue, 02 Aug 2022 13:51:52: #2 predicted fragment length is 142 bps INFO @ Tue, 02 Aug 2022 13:51:52: #2 alternative fragment length(s) may be 2,142 bps INFO @ Tue, 02 Aug 2022 13:51:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20_model.r WARNING @ Tue, 02 Aug 2022 13:51:52: #2 Since the d (142) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:51:52: #2 You may need to consider one of the other alternative d(s): 2,142 WARNING @ Tue, 02 Aug 2022 13:51:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:51:52: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:51:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:52:23: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:52:44: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:52:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10_peaks.xls INFO @ Tue, 02 Aug 2022 13:52:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:52:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.10_summits.bed INFO @ Tue, 02 Aug 2022 13:52:45: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1113 records, 4 fields): 88 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:53:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20_peaks.xls INFO @ Tue, 02 Aug 2022 13:53:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:53:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX15684317/SRX15684317.20_summits.bed INFO @ Tue, 02 Aug 2022 13:53:07: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (453 records, 4 fields): 36 millis CompletedMACS2peakCalling