Job ID = 2589490 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,890,770 reads read : 4,890,770 reads written : 4,890,770 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR503572.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:53 4890770 reads; of these: 4890770 (100.00%) were unpaired; of these: 168390 (3.44%) aligned 0 times 3986464 (81.51%) aligned exactly 1 time 735916 (15.05%) aligned >1 times 96.56% overall alignment rate Time searching: 00:00:54 Overall time: 00:00:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 268704 / 4722380 = 0.0569 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:49:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:49:07: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:49:07: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:49:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:49:08: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:49:08: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:49:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:49:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:49:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:49:15: 1000000 INFO @ Mon, 12 Aug 2019 17:49:15: 1000000 INFO @ Mon, 12 Aug 2019 17:49:16: 1000000 INFO @ Mon, 12 Aug 2019 17:49:23: 2000000 INFO @ Mon, 12 Aug 2019 17:49:23: 2000000 INFO @ Mon, 12 Aug 2019 17:49:23: 2000000 INFO @ Mon, 12 Aug 2019 17:49:30: 3000000 INFO @ Mon, 12 Aug 2019 17:49:31: 3000000 INFO @ Mon, 12 Aug 2019 17:49:31: 3000000 INFO @ Mon, 12 Aug 2019 17:49:37: 4000000 INFO @ Mon, 12 Aug 2019 17:49:38: 4000000 INFO @ Mon, 12 Aug 2019 17:49:39: 4000000 INFO @ Mon, 12 Aug 2019 17:49:40: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:49:40: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:49:40: #1 total tags in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:40: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:49:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:49:40: #1 tags after filtering in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:49:40: #1 finished! INFO @ Mon, 12 Aug 2019 17:49:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:49:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:49:40: #2 number of paired peaks: 404 WARNING @ Mon, 12 Aug 2019 17:49:40: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Mon, 12 Aug 2019 17:49:40: start model_add_line... INFO @ Mon, 12 Aug 2019 17:49:40: start X-correlation... INFO @ Mon, 12 Aug 2019 17:49:40: end of X-cor INFO @ Mon, 12 Aug 2019 17:49:40: #2 finished! INFO @ Mon, 12 Aug 2019 17:49:40: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:49:40: #2 alternative fragment length(s) may be 4,32,81,580 bps INFO @ Mon, 12 Aug 2019 17:49:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10_model.r WARNING @ Mon, 12 Aug 2019 17:49:40: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:49:40: #2 You may need to consider one of the other alternative d(s): 4,32,81,580 WARNING @ Mon, 12 Aug 2019 17:49:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:49:40: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:49:40: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:49:41: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:49:41: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:49:41: #1 total tags in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:49:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:49:41: #1 tags after filtering in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:49:41: #1 finished! INFO @ Mon, 12 Aug 2019 17:49:41: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:49:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:49:41: #2 number of paired peaks: 404 WARNING @ Mon, 12 Aug 2019 17:49:41: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Mon, 12 Aug 2019 17:49:41: start model_add_line... INFO @ Mon, 12 Aug 2019 17:49:41: start X-correlation... INFO @ Mon, 12 Aug 2019 17:49:41: end of X-cor INFO @ Mon, 12 Aug 2019 17:49:41: #2 finished! INFO @ Mon, 12 Aug 2019 17:49:41: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:49:41: #2 alternative fragment length(s) may be 4,32,81,580 bps INFO @ Mon, 12 Aug 2019 17:49:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20_model.r WARNING @ Mon, 12 Aug 2019 17:49:41: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:49:41: #2 You may need to consider one of the other alternative d(s): 4,32,81,580 WARNING @ Mon, 12 Aug 2019 17:49:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:49:41: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:49:41: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:49:42: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:49:42: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:49:42: #1 total tags in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:42: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:49:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:49:42: #1 tags after filtering in treatment: 4453676 INFO @ Mon, 12 Aug 2019 17:49:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:49:42: #1 finished! INFO @ Mon, 12 Aug 2019 17:49:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:49:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:49:43: #2 number of paired peaks: 404 WARNING @ Mon, 12 Aug 2019 17:49:43: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Mon, 12 Aug 2019 17:49:43: start model_add_line... INFO @ Mon, 12 Aug 2019 17:49:43: start X-correlation... INFO @ Mon, 12 Aug 2019 17:49:43: end of X-cor INFO @ Mon, 12 Aug 2019 17:49:43: #2 finished! INFO @ Mon, 12 Aug 2019 17:49:43: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:49:43: #2 alternative fragment length(s) may be 4,32,81,580 bps INFO @ Mon, 12 Aug 2019 17:49:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05_model.r WARNING @ Mon, 12 Aug 2019 17:49:43: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:49:43: #2 You may need to consider one of the other alternative d(s): 4,32,81,580 WARNING @ Mon, 12 Aug 2019 17:49:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:49:43: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:49:43: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:49:53: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:49:54: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:49:56: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:50:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:50:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:50:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.10_summits.bed INFO @ Mon, 12 Aug 2019 17:50:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (147 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:50:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:50:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:50:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.20_summits.bed INFO @ Mon, 12 Aug 2019 17:50:01: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (35 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:50:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:50:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:50:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX151404/SRX151404.05_summits.bed INFO @ Mon, 12 Aug 2019 17:50:02: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (383 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。