Job ID = 2589425 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,327,294 reads read : 3,327,294 reads written : 3,327,294 spots read : 3,564,992 reads read : 3,564,992 reads written : 3,564,992 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR494663.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:48 6892286 reads; of these: 6892286 (100.00%) were unpaired; of these: 5064107 (73.47%) aligned 0 times 1584842 (22.99%) aligned exactly 1 time 243337 (3.53%) aligned >1 times 26.53% overall alignment rate Time searching: 00:00:48 Overall time: 00:00:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 86165 / 1828179 = 0.0471 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:41:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:41:29: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:41:29: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:41:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:41:30: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:41:30: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:41:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:41:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:41:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:41:38: 1000000 INFO @ Mon, 12 Aug 2019 17:41:39: 1000000 INFO @ Mon, 12 Aug 2019 17:41:41: 1000000 INFO @ Mon, 12 Aug 2019 17:41:45: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:41:45: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:41:45: #1 total tags in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:41:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:41:45: #1 tags after filtering in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:41:45: #1 finished! INFO @ Mon, 12 Aug 2019 17:41:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:41:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:41:45: #2 number of paired peaks: 310 WARNING @ Mon, 12 Aug 2019 17:41:45: Fewer paired peaks (310) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 310 pairs to build model! INFO @ Mon, 12 Aug 2019 17:41:45: start model_add_line... INFO @ Mon, 12 Aug 2019 17:41:45: start X-correlation... INFO @ Mon, 12 Aug 2019 17:41:45: end of X-cor INFO @ Mon, 12 Aug 2019 17:41:45: #2 finished! INFO @ Mon, 12 Aug 2019 17:41:45: #2 predicted fragment length is 45 bps INFO @ Mon, 12 Aug 2019 17:41:45: #2 alternative fragment length(s) may be 45,509,531 bps INFO @ Mon, 12 Aug 2019 17:41:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05_model.r WARNING @ Mon, 12 Aug 2019 17:41:45: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:41:45: #2 You may need to consider one of the other alternative d(s): 45,509,531 WARNING @ Mon, 12 Aug 2019 17:41:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:41:45: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:41:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:41:46: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:41:46: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:41:46: #1 total tags in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:46: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:41:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:41:46: #1 tags after filtering in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:41:46: #1 finished! INFO @ Mon, 12 Aug 2019 17:41:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:41:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:41:46: #2 number of paired peaks: 310 WARNING @ Mon, 12 Aug 2019 17:41:46: Fewer paired peaks (310) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 310 pairs to build model! INFO @ Mon, 12 Aug 2019 17:41:46: start model_add_line... INFO @ Mon, 12 Aug 2019 17:41:46: start X-correlation... INFO @ Mon, 12 Aug 2019 17:41:46: end of X-cor INFO @ Mon, 12 Aug 2019 17:41:46: #2 finished! INFO @ Mon, 12 Aug 2019 17:41:46: #2 predicted fragment length is 45 bps INFO @ Mon, 12 Aug 2019 17:41:46: #2 alternative fragment length(s) may be 45,509,531 bps INFO @ Mon, 12 Aug 2019 17:41:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10_model.r WARNING @ Mon, 12 Aug 2019 17:41:46: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:41:46: #2 You may need to consider one of the other alternative d(s): 45,509,531 WARNING @ Mon, 12 Aug 2019 17:41:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:41:46: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:41:46: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:41:49: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:41:49: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:41:49: #1 total tags in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:41:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:41:49: #1 tags after filtering in treatment: 1742014 INFO @ Mon, 12 Aug 2019 17:41:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:41:49: #1 finished! INFO @ Mon, 12 Aug 2019 17:41:49: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:41:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:41:49: #2 number of paired peaks: 310 WARNING @ Mon, 12 Aug 2019 17:41:49: Fewer paired peaks (310) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 310 pairs to build model! INFO @ Mon, 12 Aug 2019 17:41:49: start model_add_line... INFO @ Mon, 12 Aug 2019 17:41:49: start X-correlation... INFO @ Mon, 12 Aug 2019 17:41:49: end of X-cor INFO @ Mon, 12 Aug 2019 17:41:49: #2 finished! INFO @ Mon, 12 Aug 2019 17:41:49: #2 predicted fragment length is 45 bps INFO @ Mon, 12 Aug 2019 17:41:49: #2 alternative fragment length(s) may be 45,509,531 bps INFO @ Mon, 12 Aug 2019 17:41:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20_model.r WARNING @ Mon, 12 Aug 2019 17:41:49: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:41:49: #2 You may need to consider one of the other alternative d(s): 45,509,531 WARNING @ Mon, 12 Aug 2019 17:41:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:41:49: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:41:49: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:41:50: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:41:51: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:41:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:41:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:41:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.05_summits.bed INFO @ Mon, 12 Aug 2019 17:41:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:41:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:41:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:41:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.10_summits.bed INFO @ Mon, 12 Aug 2019 17:41:54: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (32 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:41:54: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:41:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:41:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:41:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146498/SRX146498.20_summits.bed INFO @ Mon, 12 Aug 2019 17:41:57: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (7 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。