Job ID = 16432548 SRX = SRX12572573 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T00:32:23 prefetch.2.10.7: 1) Downloading 'SRR16293602'... 2022-08-02T00:32:23 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T00:37:58 prefetch.2.10.7: HTTPS download succeed 2022-08-02T00:37:58 prefetch.2.10.7: 1) 'SRR16293602' was downloaded successfully 2022-08-02T00:37:58 prefetch.2.10.7: 'SRR16293602' has 0 unresolved dependencies Read 47031001 spots for SRR16293602/SRR16293602.sra Written 47031001 spots for SRR16293602/SRR16293602.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436815 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:00:41 47031001 reads; of these: 47031001 (100.00%) were paired; of these: 4903659 (10.43%) aligned concordantly 0 times 36427525 (77.45%) aligned concordantly exactly 1 time 5699817 (12.12%) aligned concordantly >1 times ---- 4903659 pairs aligned concordantly 0 times; of these: 1718902 (35.05%) aligned discordantly 1 time ---- 3184757 pairs aligned 0 times concordantly or discordantly; of these: 6369514 mates make up the pairs; of these: 4886844 (76.72%) aligned 0 times 897167 (14.09%) aligned exactly 1 time 585503 (9.19%) aligned >1 times 94.80% overall alignment rate Time searching: 02:00:42 Overall time: 02:00:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 68 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 9896433 / 43742171 = 0.2262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:26:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:26:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:26:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:26:11: 1000000 INFO @ Tue, 02 Aug 2022 12:26:17: 2000000 INFO @ Tue, 02 Aug 2022 12:26:24: 3000000 INFO @ Tue, 02 Aug 2022 12:26:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:26:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:26:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:26:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:26:37: 5000000 INFO @ Tue, 02 Aug 2022 12:26:43: 1000000 INFO @ Tue, 02 Aug 2022 12:26:43: 6000000 INFO @ Tue, 02 Aug 2022 12:26:50: 7000000 INFO @ Tue, 02 Aug 2022 12:26:51: 2000000 INFO @ Tue, 02 Aug 2022 12:26:57: 8000000 INFO @ Tue, 02 Aug 2022 12:27:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:27:03: 9000000 INFO @ Tue, 02 Aug 2022 12:27:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:27:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:27:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:27:10: 4000000 INFO @ Tue, 02 Aug 2022 12:27:10: 10000000 INFO @ Tue, 02 Aug 2022 12:27:13: 1000000 INFO @ Tue, 02 Aug 2022 12:27:17: 11000000 INFO @ Tue, 02 Aug 2022 12:27:19: 5000000 INFO @ Tue, 02 Aug 2022 12:27:23: 2000000 INFO @ Tue, 02 Aug 2022 12:27:23: 12000000 INFO @ Tue, 02 Aug 2022 12:27:28: 6000000 INFO @ Tue, 02 Aug 2022 12:27:30: 13000000 INFO @ Tue, 02 Aug 2022 12:27:31: 3000000 INFO @ Tue, 02 Aug 2022 12:27:37: 14000000 INFO @ Tue, 02 Aug 2022 12:27:38: 7000000 INFO @ Tue, 02 Aug 2022 12:27:41: 4000000 INFO @ Tue, 02 Aug 2022 12:27:44: 15000000 INFO @ Tue, 02 Aug 2022 12:27:47: 8000000 INFO @ Tue, 02 Aug 2022 12:27:50: 5000000 INFO @ Tue, 02 Aug 2022 12:27:51: 16000000 INFO @ Tue, 02 Aug 2022 12:27:56: 9000000 INFO @ Tue, 02 Aug 2022 12:27:58: 17000000 INFO @ Tue, 02 Aug 2022 12:27:58: 6000000 INFO @ Tue, 02 Aug 2022 12:28:05: 10000000 INFO @ Tue, 02 Aug 2022 12:28:05: 18000000 INFO @ Tue, 02 Aug 2022 12:28:07: 7000000 INFO @ Tue, 02 Aug 2022 12:28:12: 19000000 INFO @ Tue, 02 Aug 2022 12:28:14: 11000000 INFO @ Tue, 02 Aug 2022 12:28:16: 8000000 INFO @ Tue, 02 Aug 2022 12:28:19: 20000000 INFO @ Tue, 02 Aug 2022 12:28:23: 12000000 INFO @ Tue, 02 Aug 2022 12:28:25: 9000000 INFO @ Tue, 02 Aug 2022 12:28:26: 21000000 INFO @ Tue, 02 Aug 2022 12:28:33: 13000000 INFO @ Tue, 02 Aug 2022 12:28:33: 22000000 INFO @ Tue, 02 Aug 2022 12:28:35: 10000000 INFO @ Tue, 02 Aug 2022 12:28:40: 23000000 INFO @ Tue, 02 Aug 2022 12:28:42: 14000000 INFO @ Tue, 02 Aug 2022 12:28:44: 11000000 INFO @ Tue, 02 Aug 2022 12:28:47: 24000000 INFO @ Tue, 02 Aug 2022 12:28:52: 15000000 INFO @ Tue, 02 Aug 2022 12:28:53: 12000000 INFO @ Tue, 02 Aug 2022 12:28:54: 25000000 INFO @ Tue, 02 Aug 2022 12:29:01: 26000000 INFO @ Tue, 02 Aug 2022 12:29:01: 16000000 INFO @ Tue, 02 Aug 2022 12:29:02: 13000000 INFO @ Tue, 02 Aug 2022 12:29:08: 27000000 INFO @ Tue, 02 Aug 2022 12:29:10: 17000000 INFO @ Tue, 02 Aug 2022 12:29:11: 14000000 INFO @ Tue, 02 Aug 2022 12:29:14: 28000000 INFO @ Tue, 02 Aug 2022 12:29:19: 18000000 INFO @ Tue, 02 Aug 2022 12:29:20: 15000000 INFO @ Tue, 02 Aug 2022 12:29:22: 29000000 INFO @ Tue, 02 Aug 2022 12:29:29: 19000000 INFO @ Tue, 02 Aug 2022 12:29:29: 30000000 INFO @ Tue, 02 Aug 2022 12:29:30: 16000000 INFO @ Tue, 02 Aug 2022 12:29:36: 31000000 INFO @ Tue, 02 Aug 2022 12:29:38: 20000000 INFO @ Tue, 02 Aug 2022 12:29:39: 17000000 INFO @ Tue, 02 Aug 2022 12:29:43: 32000000 INFO @ Tue, 02 Aug 2022 12:29:48: 21000000 INFO @ Tue, 02 Aug 2022 12:29:48: 18000000 INFO @ Tue, 02 Aug 2022 12:29:50: 33000000 INFO @ Tue, 02 Aug 2022 12:29:57: 34000000 INFO @ Tue, 02 Aug 2022 12:29:57: 22000000 INFO @ Tue, 02 Aug 2022 12:29:58: 19000000 INFO @ Tue, 02 Aug 2022 12:30:04: 35000000 INFO @ Tue, 02 Aug 2022 12:30:07: 23000000 INFO @ Tue, 02 Aug 2022 12:30:07: 20000000 INFO @ Tue, 02 Aug 2022 12:30:11: 36000000 INFO @ Tue, 02 Aug 2022 12:30:16: 24000000 INFO @ Tue, 02 Aug 2022 12:30:16: 21000000 INFO @ Tue, 02 Aug 2022 12:30:18: 37000000 INFO @ Tue, 02 Aug 2022 12:30:25: 38000000 INFO @ Tue, 02 Aug 2022 12:30:26: 22000000 INFO @ Tue, 02 Aug 2022 12:30:26: 25000000 INFO @ Tue, 02 Aug 2022 12:30:32: 39000000 INFO @ Tue, 02 Aug 2022 12:30:35: 23000000 INFO @ Tue, 02 Aug 2022 12:30:35: 26000000 INFO @ Tue, 02 Aug 2022 12:30:39: 40000000 INFO @ Tue, 02 Aug 2022 12:30:44: 27000000 INFO @ Tue, 02 Aug 2022 12:30:44: 24000000 INFO @ Tue, 02 Aug 2022 12:30:45: 41000000 INFO @ Tue, 02 Aug 2022 12:30:52: 42000000 INFO @ Tue, 02 Aug 2022 12:30:54: 28000000 INFO @ Tue, 02 Aug 2022 12:30:54: 25000000 INFO @ Tue, 02 Aug 2022 12:30:59: 43000000 INFO @ Tue, 02 Aug 2022 12:31:03: 29000000 INFO @ Tue, 02 Aug 2022 12:31:03: 26000000 INFO @ Tue, 02 Aug 2022 12:31:06: 44000000 INFO @ Tue, 02 Aug 2022 12:31:12: 30000000 INFO @ Tue, 02 Aug 2022 12:31:12: 27000000 INFO @ Tue, 02 Aug 2022 12:31:13: 45000000 INFO @ Tue, 02 Aug 2022 12:31:20: 46000000 INFO @ Tue, 02 Aug 2022 12:31:21: 28000000 INFO @ Tue, 02 Aug 2022 12:31:21: 31000000 INFO @ Tue, 02 Aug 2022 12:31:27: 47000000 INFO @ Tue, 02 Aug 2022 12:31:30: 29000000 INFO @ Tue, 02 Aug 2022 12:31:30: 32000000 INFO @ Tue, 02 Aug 2022 12:31:33: 48000000 INFO @ Tue, 02 Aug 2022 12:31:39: 30000000 INFO @ Tue, 02 Aug 2022 12:31:39: 33000000 INFO @ Tue, 02 Aug 2022 12:31:41: 49000000 INFO @ Tue, 02 Aug 2022 12:31:47: 50000000 INFO @ Tue, 02 Aug 2022 12:31:48: 31000000 INFO @ Tue, 02 Aug 2022 12:31:48: 34000000 INFO @ Tue, 02 Aug 2022 12:31:55: 51000000 INFO @ Tue, 02 Aug 2022 12:31:57: 32000000 INFO @ Tue, 02 Aug 2022 12:31:57: 35000000 INFO @ Tue, 02 Aug 2022 12:32:03: 52000000 INFO @ Tue, 02 Aug 2022 12:32:06: 33000000 INFO @ Tue, 02 Aug 2022 12:32:06: 36000000 INFO @ Tue, 02 Aug 2022 12:32:11: 53000000 INFO @ Tue, 02 Aug 2022 12:32:15: 34000000 INFO @ Tue, 02 Aug 2022 12:32:15: 37000000 INFO @ Tue, 02 Aug 2022 12:32:20: 54000000 INFO @ Tue, 02 Aug 2022 12:32:24: 35000000 INFO @ Tue, 02 Aug 2022 12:32:24: 38000000 INFO @ Tue, 02 Aug 2022 12:32:28: 55000000 INFO @ Tue, 02 Aug 2022 12:32:34: 36000000 INFO @ Tue, 02 Aug 2022 12:32:34: 39000000 INFO @ Tue, 02 Aug 2022 12:32:37: 56000000 INFO @ Tue, 02 Aug 2022 12:32:44: 40000000 INFO @ Tue, 02 Aug 2022 12:32:45: 37000000 INFO @ Tue, 02 Aug 2022 12:32:46: 57000000 INFO @ Tue, 02 Aug 2022 12:32:55: 41000000 INFO @ Tue, 02 Aug 2022 12:32:55: 58000000 INFO @ Tue, 02 Aug 2022 12:32:55: 38000000 INFO @ Tue, 02 Aug 2022 12:33:04: 59000000 INFO @ Tue, 02 Aug 2022 12:33:05: 39000000 INFO @ Tue, 02 Aug 2022 12:33:05: 42000000 INFO @ Tue, 02 Aug 2022 12:33:12: 60000000 INFO @ Tue, 02 Aug 2022 12:33:15: 40000000 INFO @ Tue, 02 Aug 2022 12:33:16: 43000000 INFO @ Tue, 02 Aug 2022 12:33:21: 61000000 INFO @ Tue, 02 Aug 2022 12:33:24: 41000000 INFO @ Tue, 02 Aug 2022 12:33:26: 44000000 INFO @ Tue, 02 Aug 2022 12:33:30: 62000000 INFO @ Tue, 02 Aug 2022 12:33:34: 42000000 INFO @ Tue, 02 Aug 2022 12:33:38: 45000000 INFO @ Tue, 02 Aug 2022 12:33:39: 63000000 INFO @ Tue, 02 Aug 2022 12:33:44: 43000000 INFO @ Tue, 02 Aug 2022 12:33:48: 46000000 INFO @ Tue, 02 Aug 2022 12:33:48: 64000000 INFO @ Tue, 02 Aug 2022 12:33:54: 44000000 INFO @ Tue, 02 Aug 2022 12:33:56: 65000000 INFO @ Tue, 02 Aug 2022 12:33:59: 47000000 INFO @ Tue, 02 Aug 2022 12:34:04: 45000000 INFO @ Tue, 02 Aug 2022 12:34:05: 66000000 INFO @ Tue, 02 Aug 2022 12:34:10: 48000000 INFO @ Tue, 02 Aug 2022 12:34:14: 67000000 INFO @ Tue, 02 Aug 2022 12:34:15: 46000000 INFO @ Tue, 02 Aug 2022 12:34:20: 49000000 INFO @ Tue, 02 Aug 2022 12:34:23: 68000000 INFO @ Tue, 02 Aug 2022 12:34:25: 47000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:34:31: 50000000 INFO @ Tue, 02 Aug 2022 12:34:32: 69000000 INFO @ Tue, 02 Aug 2022 12:34:35: 48000000 INFO @ Tue, 02 Aug 2022 12:34:35: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:34:35: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:34:35: #1 total tags in treatment: 32500278 INFO @ Tue, 02 Aug 2022 12:34:35: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:34:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:34:36: #1 tags after filtering in treatment: 27941017 INFO @ Tue, 02 Aug 2022 12:34:36: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:34:36: #1 finished! INFO @ Tue, 02 Aug 2022 12:34:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:34:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:34:38: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 12:34:38: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 12:34:38: start model_add_line... INFO @ Tue, 02 Aug 2022 12:34:38: start X-correlation... INFO @ Tue, 02 Aug 2022 12:34:38: end of X-cor INFO @ Tue, 02 Aug 2022 12:34:38: #2 finished! INFO @ Tue, 02 Aug 2022 12:34:38: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 12:34:38: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 12:34:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05_model.r WARNING @ Tue, 02 Aug 2022 12:34:38: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:34:38: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 12:34:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:34:38: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:34:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:34:42: 51000000 INFO @ Tue, 02 Aug 2022 12:34:45: 49000000 INFO @ Tue, 02 Aug 2022 12:34:52: 52000000 INFO @ Tue, 02 Aug 2022 12:34:55: 50000000 INFO @ Tue, 02 Aug 2022 12:35:03: 53000000 INFO @ Tue, 02 Aug 2022 12:35:05: 51000000 INFO @ Tue, 02 Aug 2022 12:35:13: 54000000 INFO @ Tue, 02 Aug 2022 12:35:14: 52000000 INFO @ Tue, 02 Aug 2022 12:35:24: 53000000 INFO @ Tue, 02 Aug 2022 12:35:25: 55000000 INFO @ Tue, 02 Aug 2022 12:35:25: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:35:34: 54000000 INFO @ Tue, 02 Aug 2022 12:35:35: 56000000 INFO @ Tue, 02 Aug 2022 12:35:44: 55000000 INFO @ Tue, 02 Aug 2022 12:35:45: 57000000 INFO @ Tue, 02 Aug 2022 12:35:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:35:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:35:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.05_summits.bed INFO @ Tue, 02 Aug 2022 12:35:48: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3736 records, 4 fields): 94 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:35:54: 56000000 INFO @ Tue, 02 Aug 2022 12:35:55: 58000000 INFO @ Tue, 02 Aug 2022 12:36:04: 57000000 INFO @ Tue, 02 Aug 2022 12:36:05: 59000000 INFO @ Tue, 02 Aug 2022 12:36:14: 58000000 INFO @ Tue, 02 Aug 2022 12:36:15: 60000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:36:24: 59000000 INFO @ Tue, 02 Aug 2022 12:36:26: 61000000 INFO @ Tue, 02 Aug 2022 12:36:34: 60000000 INFO @ Tue, 02 Aug 2022 12:36:36: 62000000 INFO @ Tue, 02 Aug 2022 12:36:45: 61000000 INFO @ Tue, 02 Aug 2022 12:36:46: 63000000 INFO @ Tue, 02 Aug 2022 12:36:55: 62000000 INFO @ Tue, 02 Aug 2022 12:36:56: 64000000 INFO @ Tue, 02 Aug 2022 12:37:05: 63000000 INFO @ Tue, 02 Aug 2022 12:37:06: 65000000 INFO @ Tue, 02 Aug 2022 12:37:15: 64000000 INFO @ Tue, 02 Aug 2022 12:37:16: 66000000 INFO @ Tue, 02 Aug 2022 12:37:25: 65000000 INFO @ Tue, 02 Aug 2022 12:37:26: 67000000 INFO @ Tue, 02 Aug 2022 12:37:35: 66000000 INFO @ Tue, 02 Aug 2022 12:37:36: 68000000 INFO @ Tue, 02 Aug 2022 12:37:45: 67000000 INFO @ Tue, 02 Aug 2022 12:37:46: 69000000 INFO @ Tue, 02 Aug 2022 12:37:50: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:37:50: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:37:50: #1 total tags in treatment: 32500278 INFO @ Tue, 02 Aug 2022 12:37:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:37:51: #1 tags after filtering in treatment: 27941017 INFO @ Tue, 02 Aug 2022 12:37:51: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:37:51: #1 finished! INFO @ Tue, 02 Aug 2022 12:37:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:37:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:37:53: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 12:37:53: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 12:37:53: start model_add_line... INFO @ Tue, 02 Aug 2022 12:37:53: start X-correlation... INFO @ Tue, 02 Aug 2022 12:37:53: end of X-cor INFO @ Tue, 02 Aug 2022 12:37:53: #2 finished! INFO @ Tue, 02 Aug 2022 12:37:53: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 12:37:53: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 12:37:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10_model.r WARNING @ Tue, 02 Aug 2022 12:37:53: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:37:53: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 12:37:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:37:53: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:37:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:37:55: 68000000 INFO @ Tue, 02 Aug 2022 12:38:05: 69000000 INFO @ Tue, 02 Aug 2022 12:38:09: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:38:09: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:38:09: #1 total tags in treatment: 32500278 INFO @ Tue, 02 Aug 2022 12:38:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:38:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:38:10: #1 tags after filtering in treatment: 27941017 INFO @ Tue, 02 Aug 2022 12:38:10: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:38:10: #1 finished! INFO @ Tue, 02 Aug 2022 12:38:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:38:11: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 12:38:11: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 12:38:11: start model_add_line... INFO @ Tue, 02 Aug 2022 12:38:12: start X-correlation... INFO @ Tue, 02 Aug 2022 12:38:12: end of X-cor INFO @ Tue, 02 Aug 2022 12:38:12: #2 finished! INFO @ Tue, 02 Aug 2022 12:38:12: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 12:38:12: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 12:38:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20_model.r WARNING @ Tue, 02 Aug 2022 12:38:12: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:38:12: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 12:38:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:38:12: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:38:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:38:40: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:38:58: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:39:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:39:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:39:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.10_summits.bed INFO @ Tue, 02 Aug 2022 12:39:01: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2240 records, 4 fields): 23 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:39:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:39:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:39:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572573/SRX12572573.20_summits.bed INFO @ Tue, 02 Aug 2022 12:39:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (927 records, 4 fields): 23 millis CompletedMACS2peakCalling