Job ID = 16432532 SRX = SRX12572572 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T00:32:23 prefetch.2.10.7: 1) Downloading 'SRR16293603'... 2022-08-02T00:32:23 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T00:34:11 prefetch.2.10.7: HTTPS download succeed 2022-08-02T00:34:11 prefetch.2.10.7: 1) 'SRR16293603' was downloaded successfully 2022-08-02T00:34:11 prefetch.2.10.7: 'SRR16293603' has 0 unresolved dependencies Read 46223687 spots for SRR16293603/SRR16293603.sra Written 46223687 spots for SRR16293603/SRR16293603.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436820 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 02:06:53 46223687 reads; of these: 46223687 (100.00%) were paired; of these: 5451151 (11.79%) aligned concordantly 0 times 34228406 (74.05%) aligned concordantly exactly 1 time 6544130 (14.16%) aligned concordantly >1 times ---- 5451151 pairs aligned concordantly 0 times; of these: 2140941 (39.28%) aligned discordantly 1 time ---- 3310210 pairs aligned 0 times concordantly or discordantly; of these: 6620420 mates make up the pairs; of these: 5274016 (79.66%) aligned 0 times 686725 (10.37%) aligned exactly 1 time 659679 (9.96%) aligned >1 times 94.30% overall alignment rate Time searching: 02:06:54 Overall time: 02:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 64 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8497802 / 42743801 = 0.1988 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:31:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:31:49: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:31:49: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:31:59: 1000000 INFO @ Tue, 02 Aug 2022 12:32:09: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:32:19: 3000000 INFO @ Tue, 02 Aug 2022 12:32:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:32:19: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:32:19: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:32:30: 4000000 INFO @ Tue, 02 Aug 2022 12:32:31: 1000000 INFO @ Tue, 02 Aug 2022 12:32:41: 5000000 INFO @ Tue, 02 Aug 2022 12:32:44: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:32:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:32:49: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:32:49: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:32:52: 6000000 INFO @ Tue, 02 Aug 2022 12:32:55: 3000000 INFO @ Tue, 02 Aug 2022 12:33:00: 1000000 INFO @ Tue, 02 Aug 2022 12:33:03: 7000000 INFO @ Tue, 02 Aug 2022 12:33:06: 4000000 INFO @ Tue, 02 Aug 2022 12:33:09: 2000000 INFO @ Tue, 02 Aug 2022 12:33:15: 8000000 INFO @ Tue, 02 Aug 2022 12:33:18: 5000000 INFO @ Tue, 02 Aug 2022 12:33:19: 3000000 INFO @ Tue, 02 Aug 2022 12:33:27: 9000000 INFO @ Tue, 02 Aug 2022 12:33:29: 6000000 INFO @ Tue, 02 Aug 2022 12:33:30: 4000000 INFO @ Tue, 02 Aug 2022 12:33:38: 10000000 INFO @ Tue, 02 Aug 2022 12:33:40: 5000000 INFO @ Tue, 02 Aug 2022 12:33:41: 7000000 INFO @ Tue, 02 Aug 2022 12:33:49: 11000000 INFO @ Tue, 02 Aug 2022 12:33:50: 6000000 INFO @ Tue, 02 Aug 2022 12:33:53: 8000000 INFO @ Tue, 02 Aug 2022 12:34:00: 7000000 INFO @ Tue, 02 Aug 2022 12:34:01: 12000000 INFO @ Tue, 02 Aug 2022 12:34:06: 9000000 INFO @ Tue, 02 Aug 2022 12:34:12: 8000000 INFO @ Tue, 02 Aug 2022 12:34:13: 13000000 INFO @ Tue, 02 Aug 2022 12:34:16: 10000000 INFO @ Tue, 02 Aug 2022 12:34:23: 14000000 INFO @ Tue, 02 Aug 2022 12:34:24: 9000000 INFO @ Tue, 02 Aug 2022 12:34:27: 11000000 INFO @ Tue, 02 Aug 2022 12:34:35: 10000000 INFO @ Tue, 02 Aug 2022 12:34:35: 15000000 INFO @ Tue, 02 Aug 2022 12:34:38: 12000000 INFO @ Tue, 02 Aug 2022 12:34:45: 16000000 INFO @ Tue, 02 Aug 2022 12:34:45: 11000000 INFO @ Tue, 02 Aug 2022 12:34:49: 13000000 INFO @ Tue, 02 Aug 2022 12:34:54: 17000000 INFO @ Tue, 02 Aug 2022 12:34:55: 12000000 INFO @ Tue, 02 Aug 2022 12:35:00: 14000000 INFO @ Tue, 02 Aug 2022 12:35:02: 18000000 INFO @ Tue, 02 Aug 2022 12:35:05: 13000000 INFO @ Tue, 02 Aug 2022 12:35:10: 15000000 INFO @ Tue, 02 Aug 2022 12:35:11: 19000000 INFO @ Tue, 02 Aug 2022 12:35:14: 14000000 INFO @ Tue, 02 Aug 2022 12:35:21: 20000000 INFO @ Tue, 02 Aug 2022 12:35:21: 16000000 INFO @ Tue, 02 Aug 2022 12:35:24: 15000000 INFO @ Tue, 02 Aug 2022 12:35:30: 21000000 INFO @ Tue, 02 Aug 2022 12:35:32: 17000000 INFO @ Tue, 02 Aug 2022 12:35:34: 16000000 INFO @ Tue, 02 Aug 2022 12:35:40: 22000000 INFO @ Tue, 02 Aug 2022 12:35:43: 18000000 INFO @ Tue, 02 Aug 2022 12:35:44: 17000000 INFO @ Tue, 02 Aug 2022 12:35:50: 23000000 INFO @ Tue, 02 Aug 2022 12:35:54: 19000000 INFO @ Tue, 02 Aug 2022 12:35:55: 18000000 INFO @ Tue, 02 Aug 2022 12:35:59: 24000000 INFO @ Tue, 02 Aug 2022 12:36:05: 19000000 INFO @ Tue, 02 Aug 2022 12:36:05: 20000000 INFO @ Tue, 02 Aug 2022 12:36:09: 25000000 INFO @ Tue, 02 Aug 2022 12:36:15: 20000000 INFO @ Tue, 02 Aug 2022 12:36:16: 21000000 INFO @ Tue, 02 Aug 2022 12:36:17: 26000000 INFO @ Tue, 02 Aug 2022 12:36:25: 21000000 INFO @ Tue, 02 Aug 2022 12:36:27: 27000000 INFO @ Tue, 02 Aug 2022 12:36:27: 22000000 INFO @ Tue, 02 Aug 2022 12:36:35: 22000000 INFO @ Tue, 02 Aug 2022 12:36:36: 28000000 INFO @ Tue, 02 Aug 2022 12:36:38: 23000000 INFO @ Tue, 02 Aug 2022 12:36:44: 29000000 INFO @ Tue, 02 Aug 2022 12:36:45: 23000000 INFO @ Tue, 02 Aug 2022 12:36:49: 24000000 INFO @ Tue, 02 Aug 2022 12:36:54: 30000000 INFO @ Tue, 02 Aug 2022 12:36:56: 24000000 INFO @ Tue, 02 Aug 2022 12:37:00: 25000000 INFO @ Tue, 02 Aug 2022 12:37:02: 31000000 INFO @ Tue, 02 Aug 2022 12:37:06: 25000000 INFO @ Tue, 02 Aug 2022 12:37:11: 26000000 INFO @ Tue, 02 Aug 2022 12:37:11: 32000000 INFO @ Tue, 02 Aug 2022 12:37:16: 26000000 INFO @ Tue, 02 Aug 2022 12:37:19: 33000000 INFO @ Tue, 02 Aug 2022 12:37:21: 27000000 INFO @ Tue, 02 Aug 2022 12:37:26: 27000000 INFO @ Tue, 02 Aug 2022 12:37:28: 34000000 INFO @ Tue, 02 Aug 2022 12:37:32: 28000000 INFO @ Tue, 02 Aug 2022 12:37:36: 28000000 INFO @ Tue, 02 Aug 2022 12:37:36: 35000000 INFO @ Tue, 02 Aug 2022 12:37:42: 29000000 INFO @ Tue, 02 Aug 2022 12:37:45: 36000000 INFO @ Tue, 02 Aug 2022 12:37:46: 29000000 INFO @ Tue, 02 Aug 2022 12:37:53: 30000000 INFO @ Tue, 02 Aug 2022 12:37:54: 37000000 INFO @ Tue, 02 Aug 2022 12:37:56: 30000000 INFO @ Tue, 02 Aug 2022 12:38:02: 38000000 INFO @ Tue, 02 Aug 2022 12:38:03: 31000000 INFO @ Tue, 02 Aug 2022 12:38:05: 31000000 INFO @ Tue, 02 Aug 2022 12:38:11: 39000000 INFO @ Tue, 02 Aug 2022 12:38:13: 32000000 INFO @ Tue, 02 Aug 2022 12:38:14: 32000000 INFO @ Tue, 02 Aug 2022 12:38:19: 40000000 INFO @ Tue, 02 Aug 2022 12:38:22: 33000000 INFO @ Tue, 02 Aug 2022 12:38:22: 33000000 INFO @ Tue, 02 Aug 2022 12:38:27: 41000000 INFO @ Tue, 02 Aug 2022 12:38:30: 34000000 INFO @ Tue, 02 Aug 2022 12:38:30: 34000000 INFO @ Tue, 02 Aug 2022 12:38:36: 42000000 INFO @ Tue, 02 Aug 2022 12:38:38: 35000000 INFO @ Tue, 02 Aug 2022 12:38:38: 35000000 INFO @ Tue, 02 Aug 2022 12:38:45: 43000000 INFO @ Tue, 02 Aug 2022 12:38:47: 36000000 INFO @ Tue, 02 Aug 2022 12:38:47: 36000000 INFO @ Tue, 02 Aug 2022 12:38:54: 44000000 INFO @ Tue, 02 Aug 2022 12:38:55: 37000000 INFO @ Tue, 02 Aug 2022 12:38:55: 37000000 INFO @ Tue, 02 Aug 2022 12:39:02: 45000000 INFO @ Tue, 02 Aug 2022 12:39:03: 38000000 INFO @ Tue, 02 Aug 2022 12:39:04: 38000000 INFO @ Tue, 02 Aug 2022 12:39:11: 46000000 INFO @ Tue, 02 Aug 2022 12:39:12: 39000000 INFO @ Tue, 02 Aug 2022 12:39:12: 39000000 INFO @ Tue, 02 Aug 2022 12:39:19: 47000000 INFO @ Tue, 02 Aug 2022 12:39:21: 40000000 INFO @ Tue, 02 Aug 2022 12:39:21: 40000000 INFO @ Tue, 02 Aug 2022 12:39:27: 48000000 INFO @ Tue, 02 Aug 2022 12:39:29: 41000000 INFO @ Tue, 02 Aug 2022 12:39:29: 41000000 INFO @ Tue, 02 Aug 2022 12:39:35: 49000000 INFO @ Tue, 02 Aug 2022 12:39:37: 42000000 INFO @ Tue, 02 Aug 2022 12:39:37: 42000000 INFO @ Tue, 02 Aug 2022 12:39:43: 50000000 INFO @ Tue, 02 Aug 2022 12:39:45: 43000000 INFO @ Tue, 02 Aug 2022 12:39:45: 43000000 INFO @ Tue, 02 Aug 2022 12:39:51: 51000000 INFO @ Tue, 02 Aug 2022 12:39:53: 44000000 INFO @ Tue, 02 Aug 2022 12:39:54: 44000000 INFO @ Tue, 02 Aug 2022 12:40:00: 52000000 INFO @ Tue, 02 Aug 2022 12:40:02: 45000000 INFO @ Tue, 02 Aug 2022 12:40:03: 45000000 INFO @ Tue, 02 Aug 2022 12:40:08: 53000000 INFO @ Tue, 02 Aug 2022 12:40:11: 46000000 INFO @ Tue, 02 Aug 2022 12:40:12: 46000000 INFO @ Tue, 02 Aug 2022 12:40:16: 54000000 INFO @ Tue, 02 Aug 2022 12:40:20: 47000000 INFO @ Tue, 02 Aug 2022 12:40:21: 47000000 INFO @ Tue, 02 Aug 2022 12:40:24: 55000000 INFO @ Tue, 02 Aug 2022 12:40:29: 48000000 INFO @ Tue, 02 Aug 2022 12:40:31: 48000000 INFO @ Tue, 02 Aug 2022 12:40:32: 56000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:40:38: 49000000 INFO @ Tue, 02 Aug 2022 12:40:39: 49000000 INFO @ Tue, 02 Aug 2022 12:40:41: 57000000 INFO @ Tue, 02 Aug 2022 12:40:47: 50000000 INFO @ Tue, 02 Aug 2022 12:40:49: 50000000 INFO @ Tue, 02 Aug 2022 12:40:49: 58000000 INFO @ Tue, 02 Aug 2022 12:40:56: 51000000 INFO @ Tue, 02 Aug 2022 12:40:57: 59000000 INFO @ Tue, 02 Aug 2022 12:40:58: 51000000 INFO @ Tue, 02 Aug 2022 12:41:05: 60000000 INFO @ Tue, 02 Aug 2022 12:41:05: 52000000 INFO @ Tue, 02 Aug 2022 12:41:07: 52000000 INFO @ Tue, 02 Aug 2022 12:41:13: 61000000 INFO @ Tue, 02 Aug 2022 12:41:14: 53000000 INFO @ Tue, 02 Aug 2022 12:41:16: 53000000 INFO @ Tue, 02 Aug 2022 12:41:22: 62000000 INFO @ Tue, 02 Aug 2022 12:41:23: 54000000 INFO @ Tue, 02 Aug 2022 12:41:25: 54000000 INFO @ Tue, 02 Aug 2022 12:41:30: 63000000 INFO @ Tue, 02 Aug 2022 12:41:32: 55000000 INFO @ Tue, 02 Aug 2022 12:41:34: 55000000 INFO @ Tue, 02 Aug 2022 12:41:38: 64000000 INFO @ Tue, 02 Aug 2022 12:41:41: 56000000 INFO @ Tue, 02 Aug 2022 12:41:43: 56000000 INFO @ Tue, 02 Aug 2022 12:41:46: 65000000 INFO @ Tue, 02 Aug 2022 12:41:50: 57000000 INFO @ Tue, 02 Aug 2022 12:41:52: 57000000 INFO @ Tue, 02 Aug 2022 12:41:54: 66000000 INFO @ Tue, 02 Aug 2022 12:41:59: 58000000 INFO @ Tue, 02 Aug 2022 12:42:01: 58000000 INFO @ Tue, 02 Aug 2022 12:42:03: 67000000 INFO @ Tue, 02 Aug 2022 12:42:08: 59000000 INFO @ Tue, 02 Aug 2022 12:42:10: 59000000 INFO @ Tue, 02 Aug 2022 12:42:11: 68000000 INFO @ Tue, 02 Aug 2022 12:42:17: 60000000 INFO @ Tue, 02 Aug 2022 12:42:19: 69000000 INFO @ Tue, 02 Aug 2022 12:42:20: 60000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:42:27: 61000000 INFO @ Tue, 02 Aug 2022 12:42:28: 70000000 INFO @ Tue, 02 Aug 2022 12:42:29: 61000000 INFO @ Tue, 02 Aug 2022 12:42:30: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:42:30: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:42:30: #1 total tags in treatment: 32535578 INFO @ Tue, 02 Aug 2022 12:42:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:42:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:42:30: #1 tags after filtering in treatment: 28016943 INFO @ Tue, 02 Aug 2022 12:42:30: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:42:30: #1 finished! INFO @ Tue, 02 Aug 2022 12:42:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:42:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:42:32: #2 number of paired peaks: 142 WARNING @ Tue, 02 Aug 2022 12:42:32: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Tue, 02 Aug 2022 12:42:32: start model_add_line... INFO @ Tue, 02 Aug 2022 12:42:32: start X-correlation... INFO @ Tue, 02 Aug 2022 12:42:32: end of X-cor INFO @ Tue, 02 Aug 2022 12:42:32: #2 finished! INFO @ Tue, 02 Aug 2022 12:42:32: #2 predicted fragment length is 144 bps INFO @ Tue, 02 Aug 2022 12:42:32: #2 alternative fragment length(s) may be 3,144 bps INFO @ Tue, 02 Aug 2022 12:42:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05_model.r WARNING @ Tue, 02 Aug 2022 12:42:32: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:42:32: #2 You may need to consider one of the other alternative d(s): 3,144 WARNING @ Tue, 02 Aug 2022 12:42:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:42:32: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:42:32: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:42:36: 62000000 INFO @ Tue, 02 Aug 2022 12:42:38: 62000000 INFO @ Tue, 02 Aug 2022 12:42:45: 63000000 INFO @ Tue, 02 Aug 2022 12:42:47: 63000000 INFO @ Tue, 02 Aug 2022 12:42:53: 64000000 INFO @ Tue, 02 Aug 2022 12:42:56: 64000000 INFO @ Tue, 02 Aug 2022 12:43:02: 65000000 INFO @ Tue, 02 Aug 2022 12:43:05: 65000000 INFO @ Tue, 02 Aug 2022 12:43:11: 66000000 INFO @ Tue, 02 Aug 2022 12:43:14: 66000000 INFO @ Tue, 02 Aug 2022 12:43:16: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:43:20: 67000000 INFO @ Tue, 02 Aug 2022 12:43:23: 67000000 INFO @ Tue, 02 Aug 2022 12:43:29: 68000000 INFO @ Tue, 02 Aug 2022 12:43:32: 68000000 INFO @ Tue, 02 Aug 2022 12:43:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:43:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:43:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.05_summits.bed INFO @ Tue, 02 Aug 2022 12:43:35: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2134 records, 4 fields): 73 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:43:37: 69000000 INFO @ Tue, 02 Aug 2022 12:43:42: 69000000 INFO @ Tue, 02 Aug 2022 12:43:46: 70000000 INFO @ Tue, 02 Aug 2022 12:43:48: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:43:48: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:43:48: #1 total tags in treatment: 32535578 INFO @ Tue, 02 Aug 2022 12:43:48: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:43:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:43:49: #1 tags after filtering in treatment: 28016943 INFO @ Tue, 02 Aug 2022 12:43:49: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:43:49: #1 finished! INFO @ Tue, 02 Aug 2022 12:43:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:43:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:43:50: 70000000 INFO @ Tue, 02 Aug 2022 12:43:51: #2 number of paired peaks: 142 WARNING @ Tue, 02 Aug 2022 12:43:51: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Tue, 02 Aug 2022 12:43:51: start model_add_line... INFO @ Tue, 02 Aug 2022 12:43:51: start X-correlation... INFO @ Tue, 02 Aug 2022 12:43:51: end of X-cor INFO @ Tue, 02 Aug 2022 12:43:51: #2 finished! INFO @ Tue, 02 Aug 2022 12:43:51: #2 predicted fragment length is 144 bps INFO @ Tue, 02 Aug 2022 12:43:51: #2 alternative fragment length(s) may be 3,144 bps INFO @ Tue, 02 Aug 2022 12:43:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20_model.r WARNING @ Tue, 02 Aug 2022 12:43:51: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:43:51: #2 You may need to consider one of the other alternative d(s): 3,144 WARNING @ Tue, 02 Aug 2022 12:43:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:43:51: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:43:51: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:43:52: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:43:52: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:43:52: #1 total tags in treatment: 32535578 INFO @ Tue, 02 Aug 2022 12:43:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:43:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:43:53: #1 tags after filtering in treatment: 28016943 INFO @ Tue, 02 Aug 2022 12:43:53: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:43:53: #1 finished! INFO @ Tue, 02 Aug 2022 12:43:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:43:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:43:54: #2 number of paired peaks: 142 WARNING @ Tue, 02 Aug 2022 12:43:54: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Tue, 02 Aug 2022 12:43:54: start model_add_line... INFO @ Tue, 02 Aug 2022 12:43:55: start X-correlation... INFO @ Tue, 02 Aug 2022 12:43:55: end of X-cor INFO @ Tue, 02 Aug 2022 12:43:55: #2 finished! INFO @ Tue, 02 Aug 2022 12:43:55: #2 predicted fragment length is 144 bps INFO @ Tue, 02 Aug 2022 12:43:55: #2 alternative fragment length(s) may be 3,144 bps INFO @ Tue, 02 Aug 2022 12:43:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10_model.r WARNING @ Tue, 02 Aug 2022 12:43:55: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:43:55: #2 You may need to consider one of the other alternative d(s): 3,144 WARNING @ Tue, 02 Aug 2022 12:43:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:43:55: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:43:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:44:36: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:44:39: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:44:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:44:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:44:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.20_summits.bed INFO @ Tue, 02 Aug 2022 12:44:55: Done! pass1 - making usageList (6 chroms): 3 millis pass2 - checking and writing primary data (351 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:44:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:44:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:44:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572572/SRX12572572.10_summits.bed INFO @ Tue, 02 Aug 2022 12:44:58: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1052 records, 4 fields): 42 millis CompletedMACS2peakCalling