Job ID = 16434850 SRX = SRX11633389 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 71465335 spots for SRR15329097/SRR15329097.sra Written 71465335 spots for SRR15329097/SRR15329097.sra fastq に変換しました。 bowtie でマッピング中... Your job 16435253 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:50 71465335 reads; of these: 71465335 (100.00%) were unpaired; of these: 6344459 (8.88%) aligned 0 times 56095923 (78.49%) aligned exactly 1 time 9024953 (12.63%) aligned >1 times 91.12% overall alignment rate Time searching: 00:29:50 Overall time: 00:29:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 12024330 / 65120876 = 0.1846 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:16:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:16:54: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:16:54: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:17:02: 1000000 INFO @ Tue, 02 Aug 2022 11:17:11: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:17:20: 3000000 INFO @ Tue, 02 Aug 2022 11:17:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:17:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:17:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:17:28: 4000000 INFO @ Tue, 02 Aug 2022 11:17:32: 1000000 INFO @ Tue, 02 Aug 2022 11:17:38: 5000000 INFO @ Tue, 02 Aug 2022 11:17:42: 2000000 INFO @ Tue, 02 Aug 2022 11:17:48: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:17:51: 3000000 INFO @ Tue, 02 Aug 2022 11:17:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:17:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:17:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:17:57: 7000000 INFO @ Tue, 02 Aug 2022 11:18:02: 4000000 INFO @ Tue, 02 Aug 2022 11:18:04: 1000000 INFO @ Tue, 02 Aug 2022 11:18:08: 8000000 INFO @ Tue, 02 Aug 2022 11:18:12: 5000000 INFO @ Tue, 02 Aug 2022 11:18:14: 2000000 INFO @ Tue, 02 Aug 2022 11:18:18: 9000000 INFO @ Tue, 02 Aug 2022 11:18:23: 6000000 INFO @ Tue, 02 Aug 2022 11:18:24: 3000000 INFO @ Tue, 02 Aug 2022 11:18:28: 10000000 INFO @ Tue, 02 Aug 2022 11:18:33: 7000000 INFO @ Tue, 02 Aug 2022 11:18:35: 4000000 INFO @ Tue, 02 Aug 2022 11:18:40: 11000000 INFO @ Tue, 02 Aug 2022 11:18:43: 8000000 INFO @ Tue, 02 Aug 2022 11:18:45: 5000000 INFO @ Tue, 02 Aug 2022 11:18:50: 12000000 INFO @ Tue, 02 Aug 2022 11:18:53: 9000000 INFO @ Tue, 02 Aug 2022 11:18:55: 6000000 INFO @ Tue, 02 Aug 2022 11:19:00: 13000000 INFO @ Tue, 02 Aug 2022 11:19:04: 10000000 INFO @ Tue, 02 Aug 2022 11:19:06: 7000000 INFO @ Tue, 02 Aug 2022 11:19:10: 14000000 INFO @ Tue, 02 Aug 2022 11:19:14: 11000000 INFO @ Tue, 02 Aug 2022 11:19:17: 8000000 INFO @ Tue, 02 Aug 2022 11:19:20: 15000000 INFO @ Tue, 02 Aug 2022 11:19:24: 12000000 INFO @ Tue, 02 Aug 2022 11:19:27: 9000000 INFO @ Tue, 02 Aug 2022 11:19:31: 16000000 INFO @ Tue, 02 Aug 2022 11:19:34: 13000000 INFO @ Tue, 02 Aug 2022 11:19:37: 10000000 INFO @ Tue, 02 Aug 2022 11:19:41: 17000000 INFO @ Tue, 02 Aug 2022 11:19:44: 14000000 INFO @ Tue, 02 Aug 2022 11:19:47: 11000000 INFO @ Tue, 02 Aug 2022 11:19:51: 18000000 INFO @ Tue, 02 Aug 2022 11:19:54: 15000000 INFO @ Tue, 02 Aug 2022 11:19:57: 12000000 INFO @ Tue, 02 Aug 2022 11:20:01: 19000000 INFO @ Tue, 02 Aug 2022 11:20:05: 16000000 INFO @ Tue, 02 Aug 2022 11:20:07: 13000000 INFO @ Tue, 02 Aug 2022 11:20:11: 20000000 INFO @ Tue, 02 Aug 2022 11:20:14: 17000000 INFO @ Tue, 02 Aug 2022 11:20:16: 14000000 INFO @ Tue, 02 Aug 2022 11:20:21: 21000000 INFO @ Tue, 02 Aug 2022 11:20:24: 18000000 INFO @ Tue, 02 Aug 2022 11:20:26: 15000000 INFO @ Tue, 02 Aug 2022 11:20:31: 22000000 INFO @ Tue, 02 Aug 2022 11:20:34: 19000000 INFO @ Tue, 02 Aug 2022 11:20:36: 16000000 INFO @ Tue, 02 Aug 2022 11:20:41: 23000000 INFO @ Tue, 02 Aug 2022 11:20:44: 20000000 INFO @ Tue, 02 Aug 2022 11:20:46: 17000000 INFO @ Tue, 02 Aug 2022 11:20:51: 24000000 INFO @ Tue, 02 Aug 2022 11:20:54: 21000000 INFO @ Tue, 02 Aug 2022 11:20:57: 18000000 INFO @ Tue, 02 Aug 2022 11:21:01: 25000000 INFO @ Tue, 02 Aug 2022 11:21:03: 22000000 INFO @ Tue, 02 Aug 2022 11:21:07: 19000000 INFO @ Tue, 02 Aug 2022 11:21:12: 26000000 INFO @ Tue, 02 Aug 2022 11:21:13: 23000000 INFO @ Tue, 02 Aug 2022 11:21:17: 20000000 INFO @ Tue, 02 Aug 2022 11:21:22: 27000000 INFO @ Tue, 02 Aug 2022 11:21:23: 24000000 INFO @ Tue, 02 Aug 2022 11:21:27: 21000000 INFO @ Tue, 02 Aug 2022 11:21:32: 28000000 INFO @ Tue, 02 Aug 2022 11:21:33: 25000000 INFO @ Tue, 02 Aug 2022 11:21:37: 22000000 INFO @ Tue, 02 Aug 2022 11:21:42: 29000000 INFO @ Tue, 02 Aug 2022 11:21:43: 26000000 INFO @ Tue, 02 Aug 2022 11:21:47: 23000000 INFO @ Tue, 02 Aug 2022 11:21:52: 30000000 INFO @ Tue, 02 Aug 2022 11:21:53: 27000000 INFO @ Tue, 02 Aug 2022 11:21:57: 24000000 INFO @ Tue, 02 Aug 2022 11:22:01: 31000000 INFO @ Tue, 02 Aug 2022 11:22:03: 28000000 INFO @ Tue, 02 Aug 2022 11:22:07: 25000000 INFO @ Tue, 02 Aug 2022 11:22:12: 32000000 INFO @ Tue, 02 Aug 2022 11:22:12: 29000000 INFO @ Tue, 02 Aug 2022 11:22:17: 26000000 INFO @ Tue, 02 Aug 2022 11:22:22: 30000000 INFO @ Tue, 02 Aug 2022 11:22:22: 33000000 INFO @ Tue, 02 Aug 2022 11:22:27: 27000000 INFO @ Tue, 02 Aug 2022 11:22:32: 31000000 INFO @ Tue, 02 Aug 2022 11:22:32: 34000000 INFO @ Tue, 02 Aug 2022 11:22:37: 28000000 INFO @ Tue, 02 Aug 2022 11:22:42: 32000000 INFO @ Tue, 02 Aug 2022 11:22:42: 35000000 INFO @ Tue, 02 Aug 2022 11:22:46: 29000000 INFO @ Tue, 02 Aug 2022 11:22:52: 33000000 INFO @ Tue, 02 Aug 2022 11:22:52: 36000000 INFO @ Tue, 02 Aug 2022 11:22:56: 30000000 INFO @ Tue, 02 Aug 2022 11:23:02: 37000000 INFO @ Tue, 02 Aug 2022 11:23:02: 34000000 INFO @ Tue, 02 Aug 2022 11:23:06: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 11:23:12: 38000000 INFO @ Tue, 02 Aug 2022 11:23:13: 35000000 INFO @ Tue, 02 Aug 2022 11:23:16: 32000000 INFO @ Tue, 02 Aug 2022 11:23:22: 39000000 INFO @ Tue, 02 Aug 2022 11:23:23: 36000000 INFO @ Tue, 02 Aug 2022 11:23:25: 33000000 INFO @ Tue, 02 Aug 2022 11:23:31: 40000000 INFO @ Tue, 02 Aug 2022 11:23:33: 37000000 INFO @ Tue, 02 Aug 2022 11:23:35: 34000000 INFO @ Tue, 02 Aug 2022 11:23:41: 41000000 INFO @ Tue, 02 Aug 2022 11:23:43: 38000000 INFO @ Tue, 02 Aug 2022 11:23:45: 35000000 INFO @ Tue, 02 Aug 2022 11:23:51: 42000000 INFO @ Tue, 02 Aug 2022 11:23:53: 39000000 INFO @ Tue, 02 Aug 2022 11:23:55: 36000000 INFO @ Tue, 02 Aug 2022 11:24:01: 43000000 INFO @ Tue, 02 Aug 2022 11:24:03: 40000000 INFO @ Tue, 02 Aug 2022 11:24:05: 37000000 INFO @ Tue, 02 Aug 2022 11:24:11: 44000000 INFO @ Tue, 02 Aug 2022 11:24:12: 41000000 INFO @ Tue, 02 Aug 2022 11:24:14: 38000000 INFO @ Tue, 02 Aug 2022 11:24:21: 45000000 INFO @ Tue, 02 Aug 2022 11:24:22: 42000000 INFO @ Tue, 02 Aug 2022 11:24:24: 39000000 INFO @ Tue, 02 Aug 2022 11:24:32: 46000000 INFO @ Tue, 02 Aug 2022 11:24:32: 43000000 INFO @ Tue, 02 Aug 2022 11:24:34: 40000000 INFO @ Tue, 02 Aug 2022 11:24:42: 47000000 INFO @ Tue, 02 Aug 2022 11:24:42: 44000000 INFO @ Tue, 02 Aug 2022 11:24:44: 41000000 INFO @ Tue, 02 Aug 2022 11:24:51: 45000000 INFO @ Tue, 02 Aug 2022 11:24:51: 48000000 INFO @ Tue, 02 Aug 2022 11:24:54: 42000000 INFO @ Tue, 02 Aug 2022 11:25:01: 46000000 INFO @ Tue, 02 Aug 2022 11:25:01: 49000000 INFO @ Tue, 02 Aug 2022 11:25:04: 43000000 INFO @ Tue, 02 Aug 2022 11:25:11: 47000000 INFO @ Tue, 02 Aug 2022 11:25:11: 50000000 INFO @ Tue, 02 Aug 2022 11:25:14: 44000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 11:25:21: 48000000 INFO @ Tue, 02 Aug 2022 11:25:22: 51000000 INFO @ Tue, 02 Aug 2022 11:25:24: 45000000 INFO @ Tue, 02 Aug 2022 11:25:31: 49000000 INFO @ Tue, 02 Aug 2022 11:25:32: 52000000 INFO @ Tue, 02 Aug 2022 11:25:34: 46000000 INFO @ Tue, 02 Aug 2022 11:25:41: 50000000 INFO @ Tue, 02 Aug 2022 11:25:42: 53000000 INFO @ Tue, 02 Aug 2022 11:25:44: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:25:44: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:25:44: #1 total tags in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:25:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:25:45: 47000000 INFO @ Tue, 02 Aug 2022 11:25:45: #1 tags after filtering in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:25:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:25:45: #1 finished! INFO @ Tue, 02 Aug 2022 11:25:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:25:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:25:48: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:25:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:25:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:25:51: 51000000 INFO @ Tue, 02 Aug 2022 11:25:53: 48000000 INFO @ Tue, 02 Aug 2022 11:26:00: 52000000 INFO @ Tue, 02 Aug 2022 11:26:02: 49000000 INFO @ Tue, 02 Aug 2022 11:26:09: 53000000 INFO @ Tue, 02 Aug 2022 11:26:11: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:26:11: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:26:11: #1 total tags in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:26:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:26:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:26:11: 50000000 INFO @ Tue, 02 Aug 2022 11:26:12: #1 tags after filtering in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:26:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:26:12: #1 finished! INFO @ Tue, 02 Aug 2022 11:26:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:26:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:26:17: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:26:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:26:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:26:21: 51000000 INFO @ Tue, 02 Aug 2022 11:26:29: 52000000 INFO @ Tue, 02 Aug 2022 11:26:38: 53000000 INFO @ Tue, 02 Aug 2022 11:26:39: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:26:39: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:26:39: #1 total tags in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:26:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:26:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:26:40: #1 tags after filtering in treatment: 53096546 INFO @ Tue, 02 Aug 2022 11:26:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:26:40: #1 finished! INFO @ Tue, 02 Aug 2022 11:26:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:26:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:26:44: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:26:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:26:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633389/SRX11633389.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling