Job ID = 9157372 sra ファイルのダウンロード中... Completed: 801688K bytes transferred in 8 seconds (766316K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 32584830 spots for /home/okishinya/chipatlas/results/ce10/SRX1132921/SRR2144370.sra Written 32584830 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:34 32584830 reads; of these: 32584830 (100.00%) were unpaired; of these: 1511559 (4.64%) aligned 0 times 26205813 (80.42%) aligned exactly 1 time 4867458 (14.94%) aligned >1 times 95.36% overall alignment rate Time searching: 00:12:34 Overall time: 00:12:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10906707 / 31073271 = 0.3510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:52:12: # Command line: callpeak -t SRX1132921.bam -f BAM -g ce -n SRX1132921.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132921.10 # format = BAM # ChIP-seq file = ['SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:52:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:52:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:52:12: # Command line: callpeak -t SRX1132921.bam -f BAM -g ce -n SRX1132921.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132921.05 # format = BAM # ChIP-seq file = ['SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:52:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:52:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:52:12: # Command line: callpeak -t SRX1132921.bam -f BAM -g ce -n SRX1132921.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132921.20 # format = BAM # ChIP-seq file = ['SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:52:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:52:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:52:22: 1000000 INFO @ Tue, 27 Jun 2017 11:52:22: 1000000 INFO @ Tue, 27 Jun 2017 11:52:22: 1000000 INFO @ Tue, 27 Jun 2017 11:52:32: 2000000 INFO @ Tue, 27 Jun 2017 11:52:33: 2000000 INFO @ Tue, 27 Jun 2017 11:52:33: 2000000 INFO @ Tue, 27 Jun 2017 11:52:41: 3000000 INFO @ Tue, 27 Jun 2017 11:52:42: 3000000 INFO @ Tue, 27 Jun 2017 11:52:43: 3000000 INFO @ Tue, 27 Jun 2017 11:52:50: 4000000 INFO @ Tue, 27 Jun 2017 11:52:52: 4000000 INFO @ Tue, 27 Jun 2017 11:52:53: 4000000 INFO @ Tue, 27 Jun 2017 11:52:59: 5000000 INFO @ Tue, 27 Jun 2017 11:53:02: 5000000 INFO @ Tue, 27 Jun 2017 11:53:02: 5000000 INFO @ Tue, 27 Jun 2017 11:53:08: 6000000 INFO @ Tue, 27 Jun 2017 11:53:11: 6000000 INFO @ Tue, 27 Jun 2017 11:53:11: 6000000 INFO @ Tue, 27 Jun 2017 11:53:16: 7000000 INFO @ Tue, 27 Jun 2017 11:53:20: 7000000 INFO @ Tue, 27 Jun 2017 11:53:20: 7000000 INFO @ Tue, 27 Jun 2017 11:53:25: 8000000 INFO @ Tue, 27 Jun 2017 11:53:29: 8000000 INFO @ Tue, 27 Jun 2017 11:53:29: 8000000 INFO @ Tue, 27 Jun 2017 11:53:34: 9000000 INFO @ Tue, 27 Jun 2017 11:53:38: 9000000 INFO @ Tue, 27 Jun 2017 11:53:39: 9000000 INFO @ Tue, 27 Jun 2017 11:53:42: 10000000 INFO @ Tue, 27 Jun 2017 11:53:47: 10000000 INFO @ Tue, 27 Jun 2017 11:53:48: 10000000 INFO @ Tue, 27 Jun 2017 11:53:51: 11000000 INFO @ Tue, 27 Jun 2017 11:53:56: 11000000 INFO @ Tue, 27 Jun 2017 11:53:56: 11000000 INFO @ Tue, 27 Jun 2017 11:53:59: 12000000 INFO @ Tue, 27 Jun 2017 11:54:05: 12000000 INFO @ Tue, 27 Jun 2017 11:54:05: 12000000 INFO @ Tue, 27 Jun 2017 11:54:08: 13000000 INFO @ Tue, 27 Jun 2017 11:54:14: 13000000 INFO @ Tue, 27 Jun 2017 11:54:15: 13000000 INFO @ Tue, 27 Jun 2017 11:54:16: 14000000 INFO @ Tue, 27 Jun 2017 11:54:24: 14000000 INFO @ Tue, 27 Jun 2017 11:54:25: 14000000 INFO @ Tue, 27 Jun 2017 11:54:25: 15000000 INFO @ Tue, 27 Jun 2017 11:54:35: 16000000 INFO @ Tue, 27 Jun 2017 11:54:35: 15000000 INFO @ Tue, 27 Jun 2017 11:54:36: 15000000 INFO @ Tue, 27 Jun 2017 11:54:44: 17000000 INFO @ Tue, 27 Jun 2017 11:54:47: 16000000 INFO @ Tue, 27 Jun 2017 11:54:47: 16000000 INFO @ Tue, 27 Jun 2017 11:54:54: 18000000 INFO @ Tue, 27 Jun 2017 11:54:58: 17000000 INFO @ Tue, 27 Jun 2017 11:54:58: 17000000 INFO @ Tue, 27 Jun 2017 11:55:02: 19000000 INFO @ Tue, 27 Jun 2017 11:55:07: 18000000 INFO @ Tue, 27 Jun 2017 11:55:07: 18000000 INFO @ Tue, 27 Jun 2017 11:55:10: 20000000 INFO @ Tue, 27 Jun 2017 11:55:12: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:55:12: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:55:12: #1 total tags in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:55:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:55:12: #1 tags after filtering in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:55:12: #1 finished! INFO @ Tue, 27 Jun 2017 11:55:12: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:55:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:55:13: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:55:13: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:55:13: Process for pairing-model is terminated! cat: SRX1132921.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132921.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:55:17: 19000000 INFO @ Tue, 27 Jun 2017 11:55:17: 19000000 INFO @ Tue, 27 Jun 2017 11:55:26: 20000000 INFO @ Tue, 27 Jun 2017 11:55:26: 20000000 INFO @ Tue, 27 Jun 2017 11:55:28: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:55:28: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:55:28: #1 total tags in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:28: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:55:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:55:28: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:55:28: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:55:28: #1 total tags in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:28: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:55:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:55:28: #1 tags after filtering in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:55:28: #1 finished! INFO @ Tue, 27 Jun 2017 11:55:28: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:55:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:55:28: #1 tags after filtering in treatment: 20166564 INFO @ Tue, 27 Jun 2017 11:55:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:55:28: #1 finished! INFO @ Tue, 27 Jun 2017 11:55:28: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:55:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:55:29: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:55:29: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:55:29: Process for pairing-model is terminated! cat: SRX1132921.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132921.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:55:30: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:55:30: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:55:30: Process for pairing-model is terminated! cat: SRX1132921.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132921.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132921.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。