Job ID = 9157359 sra ファイルのダウンロード中... Completed: 482257K bytes transferred in 7 seconds (556273K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29502971 spots for /home/okishinya/chipatlas/results/ce10/SRX1030169/SRR2029635.sra Written 29502971 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:50 29502971 reads; of these: 29502971 (100.00%) were unpaired; of these: 9538 (0.03%) aligned 0 times 27552826 (93.39%) aligned exactly 1 time 1940607 (6.58%) aligned >1 times 99.97% overall alignment rate Time searching: 00:05:50 Overall time: 00:05:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 1409765 / 29493433 = 0.0478 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:40:58: # Command line: callpeak -t SRX1030169.bam -f BAM -g ce -n SRX1030169.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1030169.10 # format = BAM # ChIP-seq file = ['SRX1030169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:40:58: # Command line: callpeak -t SRX1030169.bam -f BAM -g ce -n SRX1030169.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1030169.20 # format = BAM # ChIP-seq file = ['SRX1030169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:40:58: # Command line: callpeak -t SRX1030169.bam -f BAM -g ce -n SRX1030169.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1030169.05 # format = BAM # ChIP-seq file = ['SRX1030169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:41:07: 1000000 INFO @ Tue, 27 Jun 2017 11:41:07: 1000000 INFO @ Tue, 27 Jun 2017 11:41:07: 1000000 INFO @ Tue, 27 Jun 2017 11:41:15: 2000000 INFO @ Tue, 27 Jun 2017 11:41:15: 2000000 INFO @ Tue, 27 Jun 2017 11:41:15: 2000000 INFO @ Tue, 27 Jun 2017 11:41:23: 3000000 INFO @ Tue, 27 Jun 2017 11:41:23: 3000000 INFO @ Tue, 27 Jun 2017 11:41:23: 3000000 INFO @ Tue, 27 Jun 2017 11:41:31: 4000000 INFO @ Tue, 27 Jun 2017 11:41:31: 4000000 INFO @ Tue, 27 Jun 2017 11:41:31: 4000000 INFO @ Tue, 27 Jun 2017 11:41:39: 5000000 INFO @ Tue, 27 Jun 2017 11:41:39: 5000000 INFO @ Tue, 27 Jun 2017 11:41:39: 5000000 INFO @ Tue, 27 Jun 2017 11:41:47: 6000000 INFO @ Tue, 27 Jun 2017 11:41:47: 6000000 INFO @ Tue, 27 Jun 2017 11:41:47: 6000000 INFO @ Tue, 27 Jun 2017 11:41:55: 7000000 INFO @ Tue, 27 Jun 2017 11:41:55: 7000000 INFO @ Tue, 27 Jun 2017 11:41:55: 7000000 INFO @ Tue, 27 Jun 2017 11:42:03: 8000000 INFO @ Tue, 27 Jun 2017 11:42:03: 8000000 INFO @ Tue, 27 Jun 2017 11:42:03: 8000000 INFO @ Tue, 27 Jun 2017 11:42:11: 9000000 INFO @ Tue, 27 Jun 2017 11:42:11: 9000000 INFO @ Tue, 27 Jun 2017 11:42:11: 9000000 INFO @ Tue, 27 Jun 2017 11:42:19: 10000000 INFO @ Tue, 27 Jun 2017 11:42:19: 10000000 INFO @ Tue, 27 Jun 2017 11:42:19: 10000000 INFO @ Tue, 27 Jun 2017 11:42:27: 11000000 INFO @ Tue, 27 Jun 2017 11:42:27: 11000000 INFO @ Tue, 27 Jun 2017 11:42:27: 11000000 INFO @ Tue, 27 Jun 2017 11:42:35: 12000000 INFO @ Tue, 27 Jun 2017 11:42:35: 12000000 INFO @ Tue, 27 Jun 2017 11:42:35: 12000000 INFO @ Tue, 27 Jun 2017 11:42:43: 13000000 INFO @ Tue, 27 Jun 2017 11:42:43: 13000000 INFO @ Tue, 27 Jun 2017 11:42:43: 13000000 INFO @ Tue, 27 Jun 2017 11:42:51: 14000000 INFO @ Tue, 27 Jun 2017 11:42:51: 14000000 INFO @ Tue, 27 Jun 2017 11:42:51: 14000000 INFO @ Tue, 27 Jun 2017 11:42:59: 15000000 INFO @ Tue, 27 Jun 2017 11:42:59: 15000000 INFO @ Tue, 27 Jun 2017 11:42:59: 15000000 INFO @ Tue, 27 Jun 2017 11:43:07: 16000000 INFO @ Tue, 27 Jun 2017 11:43:07: 16000000 INFO @ Tue, 27 Jun 2017 11:43:07: 16000000 INFO @ Tue, 27 Jun 2017 11:43:15: 17000000 INFO @ Tue, 27 Jun 2017 11:43:15: 17000000 INFO @ Tue, 27 Jun 2017 11:43:15: 17000000 INFO @ Tue, 27 Jun 2017 11:43:23: 18000000 INFO @ Tue, 27 Jun 2017 11:43:23: 18000000 INFO @ Tue, 27 Jun 2017 11:43:23: 18000000 INFO @ Tue, 27 Jun 2017 11:43:31: 19000000 INFO @ Tue, 27 Jun 2017 11:43:31: 19000000 INFO @ Tue, 27 Jun 2017 11:43:31: 19000000 INFO @ Tue, 27 Jun 2017 11:43:39: 20000000 INFO @ Tue, 27 Jun 2017 11:43:39: 20000000 INFO @ Tue, 27 Jun 2017 11:43:39: 20000000 INFO @ Tue, 27 Jun 2017 11:43:47: 21000000 INFO @ Tue, 27 Jun 2017 11:43:47: 21000000 INFO @ Tue, 27 Jun 2017 11:43:47: 21000000 INFO @ Tue, 27 Jun 2017 11:43:55: 22000000 INFO @ Tue, 27 Jun 2017 11:43:55: 22000000 INFO @ Tue, 27 Jun 2017 11:43:55: 22000000 INFO @ Tue, 27 Jun 2017 11:44:03: 23000000 INFO @ Tue, 27 Jun 2017 11:44:03: 23000000 INFO @ Tue, 27 Jun 2017 11:44:03: 23000000 INFO @ Tue, 27 Jun 2017 11:44:11: 24000000 INFO @ Tue, 27 Jun 2017 11:44:11: 24000000 INFO @ Tue, 27 Jun 2017 11:44:11: 24000000 INFO @ Tue, 27 Jun 2017 11:44:19: 25000000 INFO @ Tue, 27 Jun 2017 11:44:19: 25000000 INFO @ Tue, 27 Jun 2017 11:44:19: 25000000 INFO @ Tue, 27 Jun 2017 11:44:27: 26000000 INFO @ Tue, 27 Jun 2017 11:44:27: 26000000 INFO @ Tue, 27 Jun 2017 11:44:27: 26000000 INFO @ Tue, 27 Jun 2017 11:44:35: 27000000 INFO @ Tue, 27 Jun 2017 11:44:35: 27000000 INFO @ Tue, 27 Jun 2017 11:44:35: 27000000 INFO @ Tue, 27 Jun 2017 11:44:43: 28000000 INFO @ Tue, 27 Jun 2017 11:44:43: 28000000 INFO @ Tue, 27 Jun 2017 11:44:43: 28000000 INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:44:44: #1 total tags in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:44:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:44:44: #1 total tags in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:44:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:44:44: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:44:44: #1 total tags in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:44:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:44:44: #1 tags after filtering in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:44:44: #1 finished! INFO @ Tue, 27 Jun 2017 11:44:44: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:44:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:44:44: #1 tags after filtering in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:44:44: #1 finished! INFO @ Tue, 27 Jun 2017 11:44:44: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:44:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:44:44: #1 tags after filtering in treatment: 28083668 INFO @ Tue, 27 Jun 2017 11:44:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:44:44: #1 finished! INFO @ Tue, 27 Jun 2017 11:44:44: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:44:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:44:46: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 11:44:46: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:44:46: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 11:44:46: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 11:44:46: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:44:46: Process for pairing-model is terminated! cat: SRX1030169.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cat: SRX1030169.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1030169.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:44:47: #2 number of paired peaks: 5 WARNING @ Tue, 27 Jun 2017 11:44:47: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:44:47: Process for pairing-model is terminated! cat: SRX1030169.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1030169.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030169.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。