Job ID = 14157876 SRX = SRX10040007 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42456586 spots for SRR13642814/SRR13642814.sra Written 42456586 spots for SRR13642814/SRR13642814.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158061 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 42456586 reads; of these: 42456586 (100.00%) were unpaired; of these: 3463823 (8.16%) aligned 0 times 31461363 (74.10%) aligned exactly 1 time 7531400 (17.74%) aligned >1 times 91.84% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7014209 / 38992763 = 0.1799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:14:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:14:59: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:14:59: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:15:04: 1000000 INFO @ Wed, 08 Dec 2021 12:15:09: 2000000 INFO @ Wed, 08 Dec 2021 12:15:14: 3000000 INFO @ Wed, 08 Dec 2021 12:15:20: 4000000 INFO @ Wed, 08 Dec 2021 12:15:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:15:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:15:29: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:15:29: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:15:31: 6000000 INFO @ Wed, 08 Dec 2021 12:15:34: 1000000 INFO @ Wed, 08 Dec 2021 12:15:37: 7000000 INFO @ Wed, 08 Dec 2021 12:15:40: 2000000 INFO @ Wed, 08 Dec 2021 12:15:43: 8000000 INFO @ Wed, 08 Dec 2021 12:15:46: 3000000 INFO @ Wed, 08 Dec 2021 12:15:48: 9000000 INFO @ Wed, 08 Dec 2021 12:15:52: 4000000 INFO @ Wed, 08 Dec 2021 12:15:54: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:15:58: 5000000 INFO @ Wed, 08 Dec 2021 12:15:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:15:59: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:15:59: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:16:00: 11000000 INFO @ Wed, 08 Dec 2021 12:16:04: 6000000 INFO @ Wed, 08 Dec 2021 12:16:05: 1000000 INFO @ Wed, 08 Dec 2021 12:16:06: 12000000 INFO @ Wed, 08 Dec 2021 12:16:10: 7000000 INFO @ Wed, 08 Dec 2021 12:16:12: 2000000 INFO @ Wed, 08 Dec 2021 12:16:12: 13000000 INFO @ Wed, 08 Dec 2021 12:16:16: 8000000 INFO @ Wed, 08 Dec 2021 12:16:17: 3000000 INFO @ Wed, 08 Dec 2021 12:16:17: 14000000 INFO @ Wed, 08 Dec 2021 12:16:22: 9000000 INFO @ Wed, 08 Dec 2021 12:16:23: 4000000 INFO @ Wed, 08 Dec 2021 12:16:23: 15000000 INFO @ Wed, 08 Dec 2021 12:16:28: 10000000 INFO @ Wed, 08 Dec 2021 12:16:28: 5000000 INFO @ Wed, 08 Dec 2021 12:16:29: 16000000 INFO @ Wed, 08 Dec 2021 12:16:34: 6000000 INFO @ Wed, 08 Dec 2021 12:16:35: 17000000 INFO @ Wed, 08 Dec 2021 12:16:35: 11000000 INFO @ Wed, 08 Dec 2021 12:16:40: 7000000 INFO @ Wed, 08 Dec 2021 12:16:40: 18000000 INFO @ Wed, 08 Dec 2021 12:16:40: 12000000 INFO @ Wed, 08 Dec 2021 12:16:46: 8000000 INFO @ Wed, 08 Dec 2021 12:16:46: 19000000 INFO @ Wed, 08 Dec 2021 12:16:46: 13000000 INFO @ Wed, 08 Dec 2021 12:16:51: 9000000 INFO @ Wed, 08 Dec 2021 12:16:52: 20000000 INFO @ Wed, 08 Dec 2021 12:16:52: 14000000 INFO @ Wed, 08 Dec 2021 12:16:57: 10000000 INFO @ Wed, 08 Dec 2021 12:16:57: 21000000 INFO @ Wed, 08 Dec 2021 12:16:58: 15000000 INFO @ Wed, 08 Dec 2021 12:17:03: 11000000 INFO @ Wed, 08 Dec 2021 12:17:03: 22000000 INFO @ Wed, 08 Dec 2021 12:17:04: 16000000 INFO @ Wed, 08 Dec 2021 12:17:09: 23000000 INFO @ Wed, 08 Dec 2021 12:17:09: 12000000 INFO @ Wed, 08 Dec 2021 12:17:10: 17000000 INFO @ Wed, 08 Dec 2021 12:17:14: 24000000 INFO @ Wed, 08 Dec 2021 12:17:15: 13000000 INFO @ Wed, 08 Dec 2021 12:17:15: 18000000 INFO @ Wed, 08 Dec 2021 12:17:20: 25000000 INFO @ Wed, 08 Dec 2021 12:17:21: 19000000 INFO @ Wed, 08 Dec 2021 12:17:21: 14000000 INFO @ Wed, 08 Dec 2021 12:17:26: 26000000 INFO @ Wed, 08 Dec 2021 12:17:27: 20000000 INFO @ Wed, 08 Dec 2021 12:17:27: 15000000 INFO @ Wed, 08 Dec 2021 12:17:31: 27000000 INFO @ Wed, 08 Dec 2021 12:17:32: 21000000 INFO @ Wed, 08 Dec 2021 12:17:34: 16000000 INFO @ Wed, 08 Dec 2021 12:17:37: 28000000 INFO @ Wed, 08 Dec 2021 12:17:38: 22000000 INFO @ Wed, 08 Dec 2021 12:17:40: 17000000 INFO @ Wed, 08 Dec 2021 12:17:42: 29000000 INFO @ Wed, 08 Dec 2021 12:17:44: 23000000 INFO @ Wed, 08 Dec 2021 12:17:46: 18000000 INFO @ Wed, 08 Dec 2021 12:17:48: 30000000 INFO @ Wed, 08 Dec 2021 12:17:49: 24000000 INFO @ Wed, 08 Dec 2021 12:17:52: 19000000 INFO @ Wed, 08 Dec 2021 12:17:54: 31000000 INFO @ Wed, 08 Dec 2021 12:17:55: 25000000 INFO @ Wed, 08 Dec 2021 12:17:59: 20000000 INFO @ Wed, 08 Dec 2021 12:18:00: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:18:00: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:18:00: #1 total tags in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:18:00: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:18:00: #1 tags after filtering in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:18:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:18:00: #1 finished! INFO @ Wed, 08 Dec 2021 12:18:00: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:18:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:18:01: 26000000 INFO @ Wed, 08 Dec 2021 12:18:02: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 12:18:02: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:18:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 12:18:05: 21000000 INFO @ Wed, 08 Dec 2021 12:18:06: 27000000 INFO @ Wed, 08 Dec 2021 12:18:11: 22000000 INFO @ Wed, 08 Dec 2021 12:18:12: 28000000 INFO @ Wed, 08 Dec 2021 12:18:18: 23000000 INFO @ Wed, 08 Dec 2021 12:18:18: 29000000 INFO @ Wed, 08 Dec 2021 12:18:23: 30000000 INFO @ Wed, 08 Dec 2021 12:18:24: 24000000 INFO @ Wed, 08 Dec 2021 12:18:29: 31000000 INFO @ Wed, 08 Dec 2021 12:18:30: 25000000 INFO @ Wed, 08 Dec 2021 12:18:34: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:18:34: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:18:34: #1 total tags in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:18:34: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:18:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:18:35: #1 tags after filtering in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:18:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:18:35: #1 finished! INFO @ Wed, 08 Dec 2021 12:18:35: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:18:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:18:36: 26000000 INFO @ Wed, 08 Dec 2021 12:18:37: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 12:18:37: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:18:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 12:18:42: 27000000 INFO @ Wed, 08 Dec 2021 12:18:48: 28000000 INFO @ Wed, 08 Dec 2021 12:18:54: 29000000 INFO @ Wed, 08 Dec 2021 12:18:59: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 12:19:05: 31000000 INFO @ Wed, 08 Dec 2021 12:19:11: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:19:11: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:19:11: #1 total tags in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:19:11: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:19:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:19:11: #1 tags after filtering in treatment: 31978554 INFO @ Wed, 08 Dec 2021 12:19:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:19:11: #1 finished! INFO @ Wed, 08 Dec 2021 12:19:11: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:19:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:19:13: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 12:19:13: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:19:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040007/SRX10040007.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling