Job ID = 2237056 sra ファイルのダウンロード中... Completed: 81397K bytes transferred in 4 seconds (144974K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 34627 0 34627 0 0 46807 0 --:--:-- --:--:-- --:--:-- 63187 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 3221462 spots for /home/okishinya/chipatlas/results/ce10/SRX094515/SRR340089.sra Written 3221462 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:18 3221462 reads; of these: 3221462 (100.00%) were unpaired; of these: 2548460 (79.11%) aligned 0 times 551471 (17.12%) aligned exactly 1 time 121531 (3.77%) aligned >1 times 20.89% overall alignment rate Time searching: 00:00:18 Overall time: 00:00:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 365749 / 673002 = 0.5435 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 30 Apr 2015 11:25:36: # Command line: callpeak -t SRX094515.bam -f BAM -g ce -n SRX094515.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX094515.20 # format = BAM # ChIP-seq file = ['SRX094515.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:36: # Command line: callpeak -t SRX094515.bam -f BAM -g ce -n SRX094515.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX094515.10 # format = BAM # ChIP-seq file = ['SRX094515.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:36: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:36: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:36: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:36: # Command line: callpeak -t SRX094515.bam -f BAM -g ce -n SRX094515.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX094515.05 # format = BAM # ChIP-seq file = ['SRX094515.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:36: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:36: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:36: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:25:38: #1 total tags in treatment: 307253 INFO @ Thu, 30 Apr 2015 11:25:38: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:25:38: #1 total tags in treatment: 307253 INFO @ Thu, 30 Apr 2015 11:25:38: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:25:38: #1 total tags in treatment: 307253 INFO @ Thu, 30 Apr 2015 11:25:38: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 307211 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 307211 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 307211 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #2 number of paired peaks: 1399 INFO @ Thu, 30 Apr 2015 11:25:38: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:38: #2 number of paired peaks: 1399 INFO @ Thu, 30 Apr 2015 11:25:38: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:38: #2 number of paired peaks: 1399 INFO @ Thu, 30 Apr 2015 11:25:38: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:39: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:39: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:39: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:39: #2 predicted fragment length is 101 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2 alternative fragment length(s) may be 101,555,590 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2.2 Generate R script for model : SRX094515.10_model.r INFO @ Thu, 30 Apr 2015 11:25:39: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:39: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:39: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:39: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:39: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:39: #2 predicted fragment length is 101 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2 alternative fragment length(s) may be 101,555,590 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2.2 Generate R script for model : SRX094515.05_model.r INFO @ Thu, 30 Apr 2015 11:25:39: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:39: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:39: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:39: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:39: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:39: #2 predicted fragment length is 101 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2 alternative fragment length(s) may be 101,555,590 bps INFO @ Thu, 30 Apr 2015 11:25:39: #2.2 Generate R script for model : SRX094515.20_model.r INFO @ Thu, 30 Apr 2015 11:25:39: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:39: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:41: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:25:41: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:25:41: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write output xls file... SRX094515.05_peaks.xls INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write output xls file... SRX094515.20_peaks.xls INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write peak in narrowPeak format file... SRX094515.20_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write peak in narrowPeak format file... SRX094515.05_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write summits bed file... SRX094515.20_summits.bed INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write summits bed file... SRX094515.05_summits.bed INFO @ Thu, 30 Apr 2015 11:25:42: Done! INFO @ Thu, 30 Apr 2015 11:25:42: Done! pass1 - making usageList (5 chroms): 1 millis pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (33 records, 4 fields): 2 millis pass2 - checking and writing primary data (146 records, 4 fields): 1 millis CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write output xls file... SRX094515.10_peaks.xls INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write peak in narrowPeak format file... SRX094515.10_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:25:42: #4 Write summits bed file... SRX094515.10_summits.bed INFO @ Thu, 30 Apr 2015 11:25:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (73 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。