Job ID = 2589300 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,469,591 reads read : 6,469,591 reads written : 6,469,591 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR217382.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:20 6469591 reads; of these: 6469591 (100.00%) were unpaired; of these: 44074 (0.68%) aligned 0 times 5406334 (83.57%) aligned exactly 1 time 1019183 (15.75%) aligned >1 times 99.32% overall alignment rate Time searching: 00:01:20 Overall time: 00:01:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 601886 / 6425517 = 0.0937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:23:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:23:35: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:23:35: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:23:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:23:36: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:23:36: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:23:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:23:37: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:23:37: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:23:44: 1000000 INFO @ Mon, 12 Aug 2019 17:23:45: 1000000 INFO @ Mon, 12 Aug 2019 17:23:46: 1000000 INFO @ Mon, 12 Aug 2019 17:23:51: 2000000 INFO @ Mon, 12 Aug 2019 17:23:52: 2000000 INFO @ Mon, 12 Aug 2019 17:23:56: 2000000 INFO @ Mon, 12 Aug 2019 17:23:57: 3000000 INFO @ Mon, 12 Aug 2019 17:24:00: 3000000 INFO @ Mon, 12 Aug 2019 17:24:04: 4000000 INFO @ Mon, 12 Aug 2019 17:24:06: 3000000 INFO @ Mon, 12 Aug 2019 17:24:07: 4000000 INFO @ Mon, 12 Aug 2019 17:24:11: 5000000 INFO @ Mon, 12 Aug 2019 17:24:15: 5000000 INFO @ Mon, 12 Aug 2019 17:24:16: 4000000 INFO @ Mon, 12 Aug 2019 17:24:16: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:24:16: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:24:16: #1 total tags in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:16: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:24:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:24:16: #1 tags after filtering in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:24:16: #1 finished! INFO @ Mon, 12 Aug 2019 17:24:16: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:24:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:24:17: #2 number of paired peaks: 396 WARNING @ Mon, 12 Aug 2019 17:24:17: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Mon, 12 Aug 2019 17:24:17: start model_add_line... INFO @ Mon, 12 Aug 2019 17:24:17: start X-correlation... INFO @ Mon, 12 Aug 2019 17:24:17: end of X-cor INFO @ Mon, 12 Aug 2019 17:24:17: #2 finished! INFO @ Mon, 12 Aug 2019 17:24:17: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:24:17: #2 alternative fragment length(s) may be 2,31,88,532,563,571,580,583 bps INFO @ Mon, 12 Aug 2019 17:24:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10_model.r WARNING @ Mon, 12 Aug 2019 17:24:17: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:24:17: #2 You may need to consider one of the other alternative d(s): 2,31,88,532,563,571,580,583 WARNING @ Mon, 12 Aug 2019 17:24:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:24:17: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:24:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:24:20: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:24:20: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:24:20: #1 total tags in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:20: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:24:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:24:21: #1 tags after filtering in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:24:21: #1 finished! INFO @ Mon, 12 Aug 2019 17:24:21: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:24:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:24:21: #2 number of paired peaks: 396 WARNING @ Mon, 12 Aug 2019 17:24:21: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Mon, 12 Aug 2019 17:24:21: start model_add_line... INFO @ Mon, 12 Aug 2019 17:24:21: start X-correlation... INFO @ Mon, 12 Aug 2019 17:24:21: end of X-cor INFO @ Mon, 12 Aug 2019 17:24:21: #2 finished! INFO @ Mon, 12 Aug 2019 17:24:21: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:24:21: #2 alternative fragment length(s) may be 2,31,88,532,563,571,580,583 bps INFO @ Mon, 12 Aug 2019 17:24:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20_model.r WARNING @ Mon, 12 Aug 2019 17:24:21: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:24:21: #2 You may need to consider one of the other alternative d(s): 2,31,88,532,563,571,580,583 WARNING @ Mon, 12 Aug 2019 17:24:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:24:21: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:24:21: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:24:26: 5000000 INFO @ Mon, 12 Aug 2019 17:24:32: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:24:32: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:24:32: #1 total tags in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:24:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:24:32: #1 tags after filtering in treatment: 5823631 INFO @ Mon, 12 Aug 2019 17:24:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:24:32: #1 finished! INFO @ Mon, 12 Aug 2019 17:24:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:24:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:24:33: #2 number of paired peaks: 396 WARNING @ Mon, 12 Aug 2019 17:24:33: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Mon, 12 Aug 2019 17:24:33: start model_add_line... INFO @ Mon, 12 Aug 2019 17:24:33: start X-correlation... INFO @ Mon, 12 Aug 2019 17:24:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:24:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:24:33: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:24:33: #2 alternative fragment length(s) may be 2,31,88,532,563,571,580,583 bps INFO @ Mon, 12 Aug 2019 17:24:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05_model.r WARNING @ Mon, 12 Aug 2019 17:24:33: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:24:33: #2 You may need to consider one of the other alternative d(s): 2,31,88,532,563,571,580,583 WARNING @ Mon, 12 Aug 2019 17:24:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:24:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:24:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:24:34: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:24:38: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:24:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:24:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:24:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.10_summits.bed INFO @ Mon, 12 Aug 2019 17:24:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (206 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:24:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:24:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:24:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.20_summits.bed INFO @ Mon, 12 Aug 2019 17:24:46: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (48 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:24:50: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:24:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:24:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:24:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065676/SRX065676.05_summits.bed INFO @ Mon, 12 Aug 2019 17:24:58: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (472 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。