Job ID = 2589199 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,786,851 reads read : 4,786,851 reads written : 4,786,851 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107588.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:49 4786851 reads; of these: 4786851 (100.00%) were unpaired; of these: 438902 (9.17%) aligned 0 times 3549489 (74.15%) aligned exactly 1 time 798460 (16.68%) aligned >1 times 90.83% overall alignment rate Time searching: 00:00:49 Overall time: 00:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 283578 / 4347949 = 0.0652 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:09:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:05: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:05: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:06: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:06: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:07: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:07: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:12: 1000000 INFO @ Mon, 12 Aug 2019 17:09:12: 1000000 INFO @ Mon, 12 Aug 2019 17:09:14: 1000000 INFO @ Mon, 12 Aug 2019 17:09:19: 2000000 INFO @ Mon, 12 Aug 2019 17:09:19: 2000000 INFO @ Mon, 12 Aug 2019 17:09:20: 2000000 INFO @ Mon, 12 Aug 2019 17:09:25: 3000000 INFO @ Mon, 12 Aug 2019 17:09:26: 3000000 INFO @ Mon, 12 Aug 2019 17:09:27: 3000000 INFO @ Mon, 12 Aug 2019 17:09:31: 4000000 INFO @ Mon, 12 Aug 2019 17:09:32: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:32: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:32: #1 total tags in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:32: #1 tags after filtering in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:32: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:32: #2 number of paired peaks: 474 WARNING @ Mon, 12 Aug 2019 17:09:32: Fewer paired peaks (474) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 474 pairs to build model! INFO @ Mon, 12 Aug 2019 17:09:32: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:32: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:32: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:32: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:32: #2 predicted fragment length is 36 bps INFO @ Mon, 12 Aug 2019 17:09:32: #2 alternative fragment length(s) may be 4,36,573 bps INFO @ Mon, 12 Aug 2019 17:09:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10_model.r WARNING @ Mon, 12 Aug 2019 17:09:32: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:09:32: #2 You may need to consider one of the other alternative d(s): 4,36,573 WARNING @ Mon, 12 Aug 2019 17:09:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:09:32: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:32: 4000000 INFO @ Mon, 12 Aug 2019 17:09:33: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:33: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:33: #1 total tags in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:33: #1 tags after filtering in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:33: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:33: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:33: #2 number of paired peaks: 474 WARNING @ Mon, 12 Aug 2019 17:09:33: Fewer paired peaks (474) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 474 pairs to build model! INFO @ Mon, 12 Aug 2019 17:09:33: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:33: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:33: #2 predicted fragment length is 36 bps INFO @ Mon, 12 Aug 2019 17:09:33: #2 alternative fragment length(s) may be 4,36,573 bps INFO @ Mon, 12 Aug 2019 17:09:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05_model.r WARNING @ Mon, 12 Aug 2019 17:09:33: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:09:33: #2 You may need to consider one of the other alternative d(s): 4,36,573 WARNING @ Mon, 12 Aug 2019 17:09:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:09:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:34: 4000000 INFO @ Mon, 12 Aug 2019 17:09:34: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:34: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:34: #1 total tags in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:34: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:34: #1 tags after filtering in treatment: 4064371 INFO @ Mon, 12 Aug 2019 17:09:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:34: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:34: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:35: #2 number of paired peaks: 474 WARNING @ Mon, 12 Aug 2019 17:09:35: Fewer paired peaks (474) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 474 pairs to build model! INFO @ Mon, 12 Aug 2019 17:09:35: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:35: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:35: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:35: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:35: #2 predicted fragment length is 36 bps INFO @ Mon, 12 Aug 2019 17:09:35: #2 alternative fragment length(s) may be 4,36,573 bps INFO @ Mon, 12 Aug 2019 17:09:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20_model.r WARNING @ Mon, 12 Aug 2019 17:09:35: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:09:35: #2 You may need to consider one of the other alternative d(s): 4,36,573 WARNING @ Mon, 12 Aug 2019 17:09:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:09:35: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:35: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:44: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:45: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:47: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.10_summits.bed INFO @ Mon, 12 Aug 2019 17:09:50: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (260 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:09:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.05_summits.bed INFO @ Mon, 12 Aug 2019 17:09:51: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (592 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:09:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044006/SRX044006.20_summits.bed INFO @ Mon, 12 Aug 2019 17:09:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (83 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。