Job ID = 2589118 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,420,329 reads read : 4,420,329 reads written : 4,420,329 spots read : 4,223,989 reads read : 4,223,989 reads written : 4,223,989 spots read : 3,083,753 reads read : 3,083,753 reads written : 3,083,753 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107302.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107303.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107304.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:18 11728071 reads; of these: 11728071 (100.00%) were unpaired; of these: 9552592 (81.45%) aligned 0 times 1902926 (16.23%) aligned exactly 1 time 272553 (2.32%) aligned >1 times 18.55% overall alignment rate Time searching: 00:01:18 Overall time: 00:01:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 105032 / 2175479 = 0.0483 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:00:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:01: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:01: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:02: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:02: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:03: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:03: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:09: 1000000 INFO @ Mon, 12 Aug 2019 17:00:10: 1000000 INFO @ Mon, 12 Aug 2019 17:00:11: 1000000 INFO @ Mon, 12 Aug 2019 17:00:16: 2000000 INFO @ Mon, 12 Aug 2019 17:00:17: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:17: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:17: #1 total tags in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:17: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:17: #1 tags after filtering in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:17: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:17: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:17: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:00:17: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:17: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:17: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:17: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:17: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:17: #2 predicted fragment length is 39 bps INFO @ Mon, 12 Aug 2019 17:00:17: #2 alternative fragment length(s) may be 39,81,560,583,586 bps INFO @ Mon, 12 Aug 2019 17:00:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05_model.r WARNING @ Mon, 12 Aug 2019 17:00:17: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:17: #2 You may need to consider one of the other alternative d(s): 39,81,560,583,586 WARNING @ Mon, 12 Aug 2019 17:00:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:17: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:17: 2000000 INFO @ Mon, 12 Aug 2019 17:00:18: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:18: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:18: #1 total tags in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:18: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:18: #1 tags after filtering in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:18: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:18: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:18: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:00:18: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:18: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:18: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:18: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:18: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:18: #2 predicted fragment length is 39 bps INFO @ Mon, 12 Aug 2019 17:00:18: #2 alternative fragment length(s) may be 39,81,560,583,586 bps INFO @ Mon, 12 Aug 2019 17:00:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10_model.r WARNING @ Mon, 12 Aug 2019 17:00:18: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:18: #2 You may need to consider one of the other alternative d(s): 39,81,560,583,586 WARNING @ Mon, 12 Aug 2019 17:00:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:18: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:18: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:18: 2000000 INFO @ Mon, 12 Aug 2019 17:00:19: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:19: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:19: #1 total tags in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:19: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:19: #1 tags after filtering in treatment: 2070447 INFO @ Mon, 12 Aug 2019 17:00:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:19: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:19: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:19: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:00:19: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:19: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:19: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:19: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:19: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:19: #2 predicted fragment length is 39 bps INFO @ Mon, 12 Aug 2019 17:00:19: #2 alternative fragment length(s) may be 39,81,560,583,586 bps INFO @ Mon, 12 Aug 2019 17:00:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20_model.r WARNING @ Mon, 12 Aug 2019 17:00:19: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:19: #2 You may need to consider one of the other alternative d(s): 39,81,560,583,586 WARNING @ Mon, 12 Aug 2019 17:00:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:19: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:19: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:23: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:24: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:25: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.05_summits.bed INFO @ Mon, 12 Aug 2019 17:00:26: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (148 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:00:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.10_summits.bed INFO @ Mon, 12 Aug 2019 17:00:27: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (44 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:00:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043847/SRX043847.20_summits.bed INFO @ Mon, 12 Aug 2019 17:00:28: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (10 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。