Job ID = 9157331 sra ファイルのダウンロード中... Completed: 518K bytes transferred in 1 seconds (2176K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25901 spots for /home/okishinya/chipatlas/results/ce10/SRX015100/SRR032446.sra Written 25901 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 25901 reads; of these: 25901 (100.00%) were unpaired; of these: 25868 (99.87%) aligned 0 times 9 (0.03%) aligned exactly 1 time 24 (0.09%) aligned >1 times 0.13% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 6 / 33 = 0.1818 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:23:26: # Command line: callpeak -t SRX015100.bam -f BAM -g ce -n SRX015100.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX015100.05 # format = BAM # ChIP-seq file = ['SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:23:26: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:23:26: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:23:26: # Command line: callpeak -t SRX015100.bam -f BAM -g ce -n SRX015100.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX015100.10 # format = BAM # ChIP-seq file = ['SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:23:26: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:23:26: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:23:26: # Command line: callpeak -t SRX015100.bam -f BAM -g ce -n SRX015100.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX015100.20 # format = BAM # ChIP-seq file = ['SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:23:26: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:23:26: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size is determined as 27 bps INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size = 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 total tags in treatment: 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size is determined as 27 bps INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size = 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 total tags in treatment: 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size is determined as 27 bps INFO @ Tue, 27 Jun 2017 11:23:26: #1 tag size = 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 total tags in treatment: 27 INFO @ Tue, 27 Jun 2017 11:23:26: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:23:26: #1 tags after filtering in treatment: 24 INFO @ Tue, 27 Jun 2017 11:23:26: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 27 Jun 2017 11:23:26: #1 finished! INFO @ Tue, 27 Jun 2017 11:23:26: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:23:26: #1 tags after filtering in treatment: 24 INFO @ Tue, 27 Jun 2017 11:23:26: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 27 Jun 2017 11:23:26: #2 number of paired peaks: 0 INFO @ Tue, 27 Jun 2017 11:23:26: #1 finished! WARNING @ Tue, 27 Jun 2017 11:23:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Tue, 27 Jun 2017 11:23:26: #2 Build Peak Model... WARNING @ Tue, 27 Jun 2017 11:23:26: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 11:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:23:26: #2 number of paired peaks: 0 INFO @ Tue, 27 Jun 2017 11:23:26: #1 tags after filtering in treatment: 24 WARNING @ Tue, 27 Jun 2017 11:23:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Tue, 27 Jun 2017 11:23:26: #1 Redundant rate of treatment: 0.11 WARNING @ Tue, 27 Jun 2017 11:23:26: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 11:23:26: #1 finished! INFO @ Tue, 27 Jun 2017 11:23:26: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:23:26: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 11:23:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:23:26: Process for pairing-model is terminated! cat: SRX015100.05_peaks.narrowPeakcat: SRX015100.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません cat: SRX015100.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX015100.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.05_model.r'rm: cannot remove `SRX015100.20_model.r': そのようなファイルやディレクトリはありませんrm: cannot remove `SRX015100.10_*.xls': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX015100.20_peaks.narrowPeak': そのようなファイルやディレクトリはありませんCompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。