Job ID = 14158432 SRX = DRX184551 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 37754672 spots for DRR194096/DRR194096.sra Written 37754672 spots for DRR194096/DRR194096.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159115 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 37754672 reads; of these: 37754672 (100.00%) were unpaired; of these: 7402876 (19.61%) aligned 0 times 20547986 (54.43%) aligned exactly 1 time 9803810 (25.97%) aligned >1 times 80.39% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13752201 / 30351796 = 0.4531 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:49:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:49:27: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:49:27: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:49:32: 1000000 INFO @ Wed, 08 Dec 2021 17:49:37: 2000000 INFO @ Wed, 08 Dec 2021 17:49:43: 3000000 INFO @ Wed, 08 Dec 2021 17:49:48: 4000000 INFO @ Wed, 08 Dec 2021 17:49:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:49:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:49:57: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:49:57: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:49:58: 6000000 INFO @ Wed, 08 Dec 2021 17:50:03: 1000000 INFO @ Wed, 08 Dec 2021 17:50:04: 7000000 INFO @ Wed, 08 Dec 2021 17:50:09: 2000000 INFO @ Wed, 08 Dec 2021 17:50:10: 8000000 INFO @ Wed, 08 Dec 2021 17:50:14: 3000000 INFO @ Wed, 08 Dec 2021 17:50:16: 9000000 INFO @ Wed, 08 Dec 2021 17:50:20: 4000000 INFO @ Wed, 08 Dec 2021 17:50:22: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:50:26: 5000000 INFO @ Wed, 08 Dec 2021 17:50:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:50:27: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:50:27: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:50:28: 11000000 INFO @ Wed, 08 Dec 2021 17:50:33: 6000000 INFO @ Wed, 08 Dec 2021 17:50:34: 1000000 INFO @ Wed, 08 Dec 2021 17:50:34: 12000000 INFO @ Wed, 08 Dec 2021 17:50:39: 7000000 INFO @ Wed, 08 Dec 2021 17:50:41: 13000000 INFO @ Wed, 08 Dec 2021 17:50:41: 2000000 INFO @ Wed, 08 Dec 2021 17:50:46: 8000000 INFO @ Wed, 08 Dec 2021 17:50:47: 14000000 INFO @ Wed, 08 Dec 2021 17:50:49: 3000000 INFO @ Wed, 08 Dec 2021 17:50:52: 9000000 INFO @ Wed, 08 Dec 2021 17:50:54: 15000000 INFO @ Wed, 08 Dec 2021 17:50:56: 4000000 INFO @ Wed, 08 Dec 2021 17:50:58: 10000000 INFO @ Wed, 08 Dec 2021 17:51:00: 16000000 INFO @ Wed, 08 Dec 2021 17:51:03: 5000000 INFO @ Wed, 08 Dec 2021 17:51:04: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:51:04: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:51:04: #1 total tags in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:51:04: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:51:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:51:04: #1 tags after filtering in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:51:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:51:04: #1 finished! INFO @ Wed, 08 Dec 2021 17:51:04: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:51:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:51:05: 11000000 INFO @ Wed, 08 Dec 2021 17:51:06: #2 number of paired peaks: 1973 INFO @ Wed, 08 Dec 2021 17:51:06: start model_add_line... INFO @ Wed, 08 Dec 2021 17:51:06: start X-correlation... INFO @ Wed, 08 Dec 2021 17:51:06: end of X-cor INFO @ Wed, 08 Dec 2021 17:51:06: #2 finished! INFO @ Wed, 08 Dec 2021 17:51:06: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 17:51:06: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 17:51:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05_model.r INFO @ Wed, 08 Dec 2021 17:51:06: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:51:06: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:51:10: 6000000 INFO @ Wed, 08 Dec 2021 17:51:11: 12000000 INFO @ Wed, 08 Dec 2021 17:51:17: 7000000 INFO @ Wed, 08 Dec 2021 17:51:18: 13000000 INFO @ Wed, 08 Dec 2021 17:51:24: 14000000 INFO @ Wed, 08 Dec 2021 17:51:24: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 17:51:31: 15000000 INFO @ Wed, 08 Dec 2021 17:51:32: 9000000 INFO @ Wed, 08 Dec 2021 17:51:37: 16000000 INFO @ Wed, 08 Dec 2021 17:51:39: 10000000 INFO @ Wed, 08 Dec 2021 17:51:41: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:51:41: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:51:41: #1 total tags in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:51:41: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:51:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:51:41: #1 tags after filtering in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:51:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:51:41: #1 finished! INFO @ Wed, 08 Dec 2021 17:51:41: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:51:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:51:42: #2 number of paired peaks: 1973 INFO @ Wed, 08 Dec 2021 17:51:42: start model_add_line... INFO @ Wed, 08 Dec 2021 17:51:42: start X-correlation... INFO @ Wed, 08 Dec 2021 17:51:42: end of X-cor INFO @ Wed, 08 Dec 2021 17:51:42: #2 finished! INFO @ Wed, 08 Dec 2021 17:51:42: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 17:51:42: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 17:51:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10_model.r INFO @ Wed, 08 Dec 2021 17:51:43: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:51:43: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:51:46: 11000000 INFO @ Wed, 08 Dec 2021 17:51:47: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:51:52: 12000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 17:51:59: 13000000 INFO @ Wed, 08 Dec 2021 17:52:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05_peaks.xls INFO @ Wed, 08 Dec 2021 17:52:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:52:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.05_summits.bed INFO @ Wed, 08 Dec 2021 17:52:05: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (19520 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:52:06: 14000000 INFO @ Wed, 08 Dec 2021 17:52:12: 15000000 INFO @ Wed, 08 Dec 2021 17:52:19: 16000000 INFO @ Wed, 08 Dec 2021 17:52:23: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:52:23: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:52:23: #1 total tags in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:52:23: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:52:23: #1 tags after filtering in treatment: 16599595 INFO @ Wed, 08 Dec 2021 17:52:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:52:23: #1 finished! INFO @ Wed, 08 Dec 2021 17:52:23: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:52:23: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:52:24: #2 number of paired peaks: 1973 INFO @ Wed, 08 Dec 2021 17:52:24: start model_add_line... INFO @ Wed, 08 Dec 2021 17:52:24: start X-correlation... INFO @ Wed, 08 Dec 2021 17:52:24: end of X-cor INFO @ Wed, 08 Dec 2021 17:52:24: #2 finished! INFO @ Wed, 08 Dec 2021 17:52:24: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 17:52:24: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 17:52:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20_model.r INFO @ Wed, 08 Dec 2021 17:52:24: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:52:24: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:52:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10_peaks.xls INFO @ Wed, 08 Dec 2021 17:52:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:52:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.10_summits.bed INFO @ Wed, 08 Dec 2021 17:52:41: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (16995 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:53:05: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:53:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20_peaks.xls INFO @ Wed, 08 Dec 2021 17:53:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:53:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184551/DRX184551.20_summits.bed INFO @ Wed, 08 Dec 2021 17:53:22: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (13075 records, 4 fields): 13 millis CompletedMACS2peakCalling