Job ID = 14158420 SRX = DRX184547 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25716567 spots for DRR194092/DRR194092.sra Written 25716567 spots for DRR194092/DRR194092.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159098 ("srTce11") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 25716567 reads; of these: 25716567 (100.00%) were unpaired; of these: 1961385 (7.63%) aligned 0 times 14668947 (57.04%) aligned exactly 1 time 9086235 (35.33%) aligned >1 times 92.37% overall alignment rate Time searching: 00:06:41 Overall time: 00:06:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10917133 / 23755182 = 0.4596 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:44:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:44:33: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:44:33: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:44:38: 1000000 INFO @ Wed, 08 Dec 2021 17:44:43: 2000000 INFO @ Wed, 08 Dec 2021 17:44:48: 3000000 INFO @ Wed, 08 Dec 2021 17:44:53: 4000000 INFO @ Wed, 08 Dec 2021 17:44:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:45:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:45:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:45:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:45:03: 6000000 INFO @ Wed, 08 Dec 2021 17:45:09: 7000000 INFO @ Wed, 08 Dec 2021 17:45:09: 1000000 INFO @ Wed, 08 Dec 2021 17:45:15: 8000000 INFO @ Wed, 08 Dec 2021 17:45:16: 2000000 INFO @ Wed, 08 Dec 2021 17:45:21: 9000000 INFO @ Wed, 08 Dec 2021 17:45:23: 3000000 INFO @ Wed, 08 Dec 2021 17:45:27: 10000000 INFO @ Wed, 08 Dec 2021 17:45:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:45:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:45:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:45:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:45:33: 11000000 INFO @ Wed, 08 Dec 2021 17:45:36: 5000000 INFO @ Wed, 08 Dec 2021 17:45:39: 12000000 INFO @ Wed, 08 Dec 2021 17:45:39: 1000000 INFO @ Wed, 08 Dec 2021 17:45:43: 6000000 INFO @ Wed, 08 Dec 2021 17:45:44: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:45:44: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:45:44: #1 total tags in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:45:44: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:45:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:45:44: #1 tags after filtering in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:45:44: #1 finished! INFO @ Wed, 08 Dec 2021 17:45:44: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:45:45: #2 number of paired peaks: 2563 INFO @ Wed, 08 Dec 2021 17:45:45: start model_add_line... INFO @ Wed, 08 Dec 2021 17:45:45: start X-correlation... INFO @ Wed, 08 Dec 2021 17:45:45: end of X-cor INFO @ Wed, 08 Dec 2021 17:45:45: #2 finished! INFO @ Wed, 08 Dec 2021 17:45:45: #2 predicted fragment length is 182 bps INFO @ Wed, 08 Dec 2021 17:45:45: #2 alternative fragment length(s) may be 182 bps INFO @ Wed, 08 Dec 2021 17:45:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05_model.r INFO @ Wed, 08 Dec 2021 17:45:45: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:45:45: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:45:47: 2000000 INFO @ Wed, 08 Dec 2021 17:45:50: 7000000 INFO @ Wed, 08 Dec 2021 17:45:54: 3000000 INFO @ Wed, 08 Dec 2021 17:45:56: 8000000 INFO @ Wed, 08 Dec 2021 17:46:00: 4000000 INFO @ Wed, 08 Dec 2021 17:46:03: 9000000 INFO @ Wed, 08 Dec 2021 17:46:07: 5000000 INFO @ Wed, 08 Dec 2021 17:46:10: 10000000 INFO @ Wed, 08 Dec 2021 17:46:14: 6000000 INFO @ Wed, 08 Dec 2021 17:46:17: 11000000 INFO @ Wed, 08 Dec 2021 17:46:17: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:46:20: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 17:46:23: 12000000 INFO @ Wed, 08 Dec 2021 17:46:27: 8000000 INFO @ Wed, 08 Dec 2021 17:46:29: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:46:29: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:46:29: #1 total tags in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:46:29: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:46:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:46:29: #1 tags after filtering in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:46:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:46:29: #1 finished! INFO @ Wed, 08 Dec 2021 17:46:29: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:46:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:46:30: #2 number of paired peaks: 2563 INFO @ Wed, 08 Dec 2021 17:46:30: start model_add_line... INFO @ Wed, 08 Dec 2021 17:46:30: start X-correlation... INFO @ Wed, 08 Dec 2021 17:46:30: end of X-cor INFO @ Wed, 08 Dec 2021 17:46:30: #2 finished! INFO @ Wed, 08 Dec 2021 17:46:30: #2 predicted fragment length is 182 bps INFO @ Wed, 08 Dec 2021 17:46:30: #2 alternative fragment length(s) may be 182 bps INFO @ Wed, 08 Dec 2021 17:46:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10_model.r INFO @ Wed, 08 Dec 2021 17:46:30: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:46:30: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:46:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05_peaks.xls INFO @ Wed, 08 Dec 2021 17:46:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:46:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.05_summits.bed INFO @ Wed, 08 Dec 2021 17:46:31: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (18768 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:46:33: 9000000 INFO @ Wed, 08 Dec 2021 17:46:40: 10000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 17:46:46: 11000000 INFO @ Wed, 08 Dec 2021 17:46:53: 12000000 INFO @ Wed, 08 Dec 2021 17:46:58: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:46:58: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:46:58: #1 total tags in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:46:58: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:46:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:46:58: #1 tags after filtering in treatment: 12838049 INFO @ Wed, 08 Dec 2021 17:46:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:46:58: #1 finished! INFO @ Wed, 08 Dec 2021 17:46:58: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:46:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:46:59: #2 number of paired peaks: 2563 INFO @ Wed, 08 Dec 2021 17:46:59: start model_add_line... INFO @ Wed, 08 Dec 2021 17:46:59: start X-correlation... INFO @ Wed, 08 Dec 2021 17:46:59: end of X-cor INFO @ Wed, 08 Dec 2021 17:46:59: #2 finished! INFO @ Wed, 08 Dec 2021 17:46:59: #2 predicted fragment length is 182 bps INFO @ Wed, 08 Dec 2021 17:46:59: #2 alternative fragment length(s) may be 182 bps INFO @ Wed, 08 Dec 2021 17:46:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20_model.r INFO @ Wed, 08 Dec 2021 17:46:59: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:46:59: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:47:02: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:47:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10_peaks.xls INFO @ Wed, 08 Dec 2021 17:47:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:47:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.10_summits.bed INFO @ Wed, 08 Dec 2021 17:47:15: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (16077 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:47:29: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:47:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20_peaks.xls INFO @ Wed, 08 Dec 2021 17:47:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:47:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184547/DRX184547.20_summits.bed INFO @ Wed, 08 Dec 2021 17:47:43: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (12018 records, 4 fields): 13 millis CompletedMACS2peakCalling