AcEST

2010/02/15

WebSite: http://togodb.dbcls.jp/acest/
FTP Site: ftp://ftp.biosciencedbc.jp/archive/acest

This is a database of EST sequences of Adiantum capillus-veneris determined by Tokyo Metropolitan University, and the results of the blastx search between these sequences and UniProtKB/Swiss-Prot and TrEMBL databases. Although various plant genomes have been analyzed, the research concerning fern plants has not progressed among land plants (including seed plants, fern and moss). Considering Adiantum capillus-veneris, which has been used for photomorphogenesis research, as a model fern plant, EST sequence analysis of Adiantum was performed.

README Content

  1. Database Component
  2. Data Description
  3. License
  4. Update History
  5. Literature
  6. Contact address

1. Database Component

  1. README
  2. AcEST(EST sequences of Adiantum capillus-veneris and their annotation)
  3. BlastX Result : Swiss-Prot
  4. BlastX Result : TrEMBL
  5. AcEST Contig
  6. About Libraries
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2. Data Description

2.1 README

Data name README
Description of data contents HTML file to describe "AcEST" data.
File README_e.html(English)
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2.2 AcEST(EST sequences of Adiantum capillus-veneris and their annotation)

Data name AcEST
Description of data contents EST sequence of Adiantum capillus-veneris and its annotation (clone ID, library name, Blastx search results, etc.) CSV format text file.
File acest_est.zip (110MB)
Data items are the following:
Data item Description
Clone id Clone ID of EST sequence of Adiantum capillus-veneris.
Library Library name
Length Number of bases
Definition Definition
Accession DDBJ accession number
Tissue type Name of the tissue from which the sequence is derived
Developmental stage Developmental stage
Sequence(60) Nucleotide sequence (line break after 60 characters)
sequence Nucleotide sequence (without line break)
||Homology search results||  
Swiss-Prot (release 56.9) Results of blastx search against Swiss-Prot (release 56.9)
UniProt ID of top hit in Swiss-Prot UniProt ID of the hit sequence with the highest score in the blastx search against Swiss-Prot (release 56.9)
Definition Accession and definition of the hit sequence with the highest score in the blastx search against Swiss-Prot (release 56.9). Link to Uniprot is provided in TogoDB.
Align length The alignment length between the subject and the hit sequence with the highest score in the blastx search against Swiss-Prot (release 56.9).
Score (bit) Score (bit) of the alignment between the subject and the hit sequence with the highest score in the blastx search against Swiss-Prot (release 56.9).
E-value E-value of the alignment between the subject and the hit sequence with the highest score in the blastx search against Swiss-Prot (release 56.9).
Report Results of blastx search against the UniProtKB/Swiss-Prot (release 56.9) database.
TrEMBL (release 39.9) Results of blastx search against TrEMBL (release 39.9)
UniProt ID of top hit in TrEMBL UniProt ID of the hit sequence with the highest score in the blastx search against TrEMBL (release 39.9).
Definition Accession and definition of the hit sequence with the highest score in the blastx search against TrEMBL (release 39.9). Link to Uniprot is provided in TogoDB.
Align length The alignment length between the subject and the hit sequence with the highest score in the blastx search against TrEMBL (release 39.9).
Score (bit) Score (bit) of the alignment between the subject and the hit sequence with the highest score in the blastx search against TrEMBL (release 39.9).
E-value E-value of the alignment between the subject and the hit sequence with the highest score in the blastx search against TrEMBL (release 39.9).
Report Results of blastx search against the UniProtKB/TrEMBL (release 39.9) database.

2.3 BlastX Result : Swiss-Prot

Data name BlastX Result : Swiss-Prot
Description of data contents Results of the blastx search of Adiantum capillus-veneris EST (AcEST) sequences against the UniProtKB/Swiss-Prot (release 56.9) database. The alignment information of each hit in the Blastx hit list is provided on a single line. CSV format text file.
File blasthits_sp.zip (7.77MB)
Data items are the following:
Data item Description
Query Clone ID of Adiantum capillus-veneris EST sequence used for query
BlastX Hits Accession and definition of the hit sequence in the blastx search against the UniProtKB/Swiss-Prot (release 56.9) database. Link to Uniprot is provided in TogoDB.
Align Length The alignment length between the above hit sequence and the query sequence
Bit score Score (bit) of the alignment between the above hit sequence and the qury sequence
E-value E-value of the alignment between the above hit sequence and the query sequence
Report Results of blastx search against the UniProtKB/Swiss-Prot (release 56.9) database.
UniProt ID UniProt ID of the above hit sequence

2.4 BlastX Result : TrEMBL

Data name BlastX Result : TrEMBL
Description of data contents Results of the blastx search of Adiantum capillus-veneris EST (AcEST) sequences against the UniProtKB/TrEMBL (release 39.9) database. The alignment information of each hit in the Blastx hit list is provided on a single line. CSV format text file.
File blasthits_tr.zip (7.03MB)
Data items are the following:
Data item Description
Query Clone ID of Adiantum capillus-veneris EST sequence subjected to the blastX search
BlastX Hits Accession and definition of the hit sequence in the blastx search against the UniProtKB/TrEMBL (release 39.9) database. Link to Uniprot is provided in TogoDB.
Align Length The alignment length between the above hit sequence and an Adiantum capillus-veneris EST sequence
Bit score Score (bit) of the alignment between the above hit sequence and an Adiantum capillus-veneris EST sequence
E-value E-value of the alignment between the above hit sequence and an Adiantum capillus-veneris EST sequence
Report Results of blastx search against the UniProtKB/TrEMBL (release 39.9) database
UniProt ID UniProt ID of the above hit sequence

2.5  AcEST Contig

Data name AcEST Contig
Description of data contents Information on the contig sequence comprising Adiantum capillus-veneris EST (AcEST). CSV format text file.
File acestcontig.zip (1.34MB)
Data items are the following:
Data item Description
Contig ID ID of contig sequence of Adiantum capillus-veneris
Length Length of the contig sequence of Adiantum capillus-veneris
Number of clones The number of EST clones constituting the contig sequence of Adiantum capillus-veneris
Definition Definition
Link to clone list In the TogoDB edition, link to a list of EST clones constituting the contig sequence of Adiantum capillus-veneris is provided. It is blank in the downloaded file.
Clone ID ID of the EST clone constituting the contig sequence of Adiantum capillus-veneris with link to the AcEST clone information
Sequence Nucleotide sequence

2.6 About Libraries

Data name About Libraries
Description of data contents Information concerning cDNA library of Adiantum capillus-veneris EST (AcEST). CSV format text file.
File acest_lib.zip (2KB)
Data items are the following:
Data item Description
Name Name of Adiantum capillus-veneris EST library
About Adiantum capillus-veneris EST library creation information
References References of creation of Adiantum capillus-veneris EST library
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3. License

The Standard License specifies the license terms regarding the use of this database and the requirements you must follow in using this database.
The Additional License specifies those items that are exceptionally permitted even though they are generally prohibited in the Standard License.

3.1 Standard License

The Standard License for this database is the license specified in the Creative Commons Attribution-Share Alike 2.1 Japan.
If you use data from this database, please be sure attribute this database as follows: "AcEST, Copyright© 2009 Takeshi Kanegae(Tokyo Metropolitan University) licensed under CC Attribution-Share Alike 2.1 Japan".

The summary of the Creative Commons Attribution-Share Alike 2.1 Japan is found here.

With regard to this database, you are licensed to:

  1. freely access part or whole of this database, and acquire data;
  2. freely redistribute part or whole of the data from this database; and
  3. freely create and distribute database and other derivative works based on part or whole of the data from this database,

under the Standard License, as long as you comply with the following conditions:

  1. You must attribute this database in the manner specified by the author or licensor when distributing part or whole of this database or any derivative work.
  2. You must distribute any derivative work based on part or whole of the data from this database under this License.

3.2 Additional License

1. You must display this Additional License along with the Standard License when distributing any derivative work based on part of whole of the data from this database.

2. When you conduct research by using this database, and describe the research results in an article or paper, you always need to cite this database, and specify the name and URL of this database in the article or paper.

3. When distributing any derivative database based on this database, you must not use the name of this database or any part of it as the name of derivative database.

4.You need to contact the Licensor shown below to request a license for use of this database or any part thereof not licensed under the Standard License and the above Additional License.

Plant Environmental Responses Laboratory, Department of Biological Sciences, Tokyo Metropolitan University Graduate School of Science and Engineering
1-1 Minamiosawa, Hachioji-shi, Tokyo-to
192-0397
Tel: +81-42-677-1111(ext.3654)
E-mail: tkanegae[at]tmu[dot]ac[dot]jp

3.3 About Providing Links to This Database

You can freely provide links to all contents in this database. But, contents might be changed without notice.

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4. Update History

Date Update contents
2010/03/29 AcEST english archive site is opened.
2009/6/12 AcEST(http://togodb.dbcls.jp/acest/) is released.
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5. Literature

Yamauchi, D., Sutoh, K., Kanegae, H., Horiguchi, T., Matsuoka, K., Fukuda, H., Wada, M.
Analysis of expressed sequence tags in prothallia of Adiantum capillus-veneris.
J. Plant Res. 118 (3): 223-227.
PMID: 15940394

6. Contact address

When you have any question about "AcEST", contact the following:

Plant Environmental Responses Laboratory, Department of Biological Sciences, Tokyo Metropolitan University Graduate School of Science and Engineering
1-1 Minamiosawa, Hachioji-shi, Tokyo-to
192-0397
Tel: +81-42-677-1111(ext.3654)
E-mail: tkanegae[at]tmu[dot]ac[dot]jp

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