PMID: 7744810,7584498,3260265 Numerous exogenous agents and endogenous proinflammatory stimulants, such as endotoxin, lectins, hypoxia, viruses, bacteria, IL-1, and TNF-alpha, stimulate IL-8 production in a wide variety of cell types,by stabilization of IL-8 mRNA as well as by activation of IL-8 gene transcription PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8.

PMID: 7744810 Analysis of the IL-8 promoter showed that the sequences between positions -91 and -71 contain the NF-KappaB-binding site and the NF-IL-6 (or C/EBP) site and are sufficient for the induction of IL-8 gene transcription by LPS, IL-1, TNF-alpha, phorbol 12-myristate 13-acetate, or hepatitis B virus protein X. PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8.

PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8.

PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8.

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,2217207 IL-1 and TNF-alpha stimulate the expression of many of the alpha-chemokines including GROalpha, GRObeta (MIP-2alpha), and GROgamma (MIP-2 beta) in monocytes, fibroblasts, endothelial cells, and mammary epithelial cells through NF-KappaB sites in their promoters

PMID: 7744810,7584498,7929219,8083342 ENA-78, one of the neutrophil attractant chemokines, is produced and secreted by epithelial cells in response to IL-1beta or TNF-alpha, along with IL-8, GROalpha, and GROgamma.

PMID: 7744810,7584498,3260265 Numerous exogenous agents and endogenous proinflammatory stimulants, such as endotoxin, lectins, hypoxia, viruses, bacteria, IL-1, and TNF-alpha, stimulate IL-8 production in a wide variety of cell types,by stabilization of IL-8 mRNA as well as by activation of IL-8 gene transcription PMID: 7744810 Analysis of the IL-8 promoter showed that the sequences between positions -91 and -71 contain the NF-KappaB-binding site and the NF-IL-6 (or C/EBP) site and are sufficient for the induction of IL-8 gene transcription by LPS, IL-1, TNF-alpha, phorbol 12-myristate 13-acetate, or hepatitis B virus protein X. PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8. PMID: 7744810,8175759 However, dexamethasone also suppresses IL-1-induced IL-8 production through the NF-kappaB site

PMID: 7744810,7584498,7929219,8083342 ENA-78, one of the neutrophil attractant chemokines, is produced and secreted by epithelial cells in response to IL-1beta or TNF-alpha, along with IL-8, GROalpha, and GROgamma

PMID: 7744810,7584498,7929219,8083342 ENA-78, one of the neutrophil attractant chemokines, is produced and secreted by epithelial cells in response to IL-1beta or TNF-alpha, along with IL-8, GROalpha, and GROgamma

PMID: 7744810,8454640 IFN-gamma induces IP-10 expression through an ISRE element present in the region flanking the transcription start site PMID: 7744810,8051417 IL-4 can inhibit IFN-gamma induction by activation of a negative regulator that competes for the ISRE site

PMID: 7744810,8355682 NF-IL-6 (C/EPB), NF-KappaB, and c-Ets sites have been identified in the promoter for MIP-1alpha

PMID: 7744810,8355682 NF-IL-6 (C/EPB), NF-KappaB, and c-Ets sites have been identified in the promoter for MIP-1alpha

PMID: 7744810 Studies of the regulation of neutrophil expression of CCRs show that a 30-min incubation of neutrophils with granulocyte-macrophage colony-stimulating factor down-regulates the expression of IL-8R and C5a receptor (C5aR) and up-regulates fMLP receptor (fMLPR) expression

PMID: 7744810 Studies of the regulation of neutrophil expression of CCRs show that a 30-min incubation of neutrophils with granulocyte-macrophage colony-stimulating factor down-regulates the expression of IL-8R and C5a receptor (C5aR) and up-regulates fMLP receptor (fMLPR) expression

PMID: 7744810 Studies of the regulation of neutrophil expression of CCRs show that a 30-min incubation of neutrophils with granulocyte-macrophage colony-stimulating factor down-regulates the expression of IL-8R and C5a receptor (C5aR) and up-regulates fMLP receptor (fMLPR) expression

PMID: 7744810 In addition, in vitro incubation of neutrophils with granulocyte colony-stimulating factor enhances, whereas LPS inhibits, the expression of IL-8R mRNA, IL-8 binding, and chemotactic responses by neutrophils

PMID: 7744810 In addition, in vitro incubation of neutrophils with granulocyte colony-stimulating factor enhances, whereas LPS inhibits, the expression of IL-8R mRNA, IL-8 binding, and chemotactic responses by neutrophils

PMID: 7744810,7584498,3260265 Numerous exogenous agents and endogenous proinflammatory stimulants, such as endotoxin, lectins, hypoxia, viruses, bacteria, IL-1, and TNF-alpha, stimulate IL-8 production in a wide variety of cell types,by stabilization of IL-8 mRNA as well as by activation of IL-8 gene transcription PMID: 7744810 Analysis of the IL-8 promoter showed that the sequences between positions -91 and -71 contain the NF-KappaB-binding site and the NF-IL-6 (or C/EBP) site and are sufficient for the induction of IL-8 gene transcription by LPS, IL-1, TNF-alpha, phorbol 12-myristate 13-acetate, or hepatitis B virus protein X. PMID: 7744810 Glucocorticoids, such as dexamethasone, are potent immunomodulating anti-inflammatory agents that have a marked inhibitory effect on the gene transcription of several proinflammatory cytokines including IL-1, TNF-alpha, and IL-8. PMID: 7744810,8035808 Interferon- beta also appears to regulate inflammatory cytokine transcription as it inhibits TNF-alpha-induced IL-8 gene expression at the transcriptional level via the NF-KappaB site

PMID: 7744810 In contrast, LPS up-regulates the expression of neutrophil fMLP receptors by increasing gene transcription

PMID: 7744810 Studies on IL-8RA and IL-8RB show that although both receptors bind IL-8 with similar affinities, the ligand-receptor interaction to each of the receptors is mediated through different regions of IL-8.

PMID: 7744810 Studies on IL-8RA and IL-8RB show that although both receptors bind IL-8 with similar affinities, the ligand-receptor interaction to each of the receptors is mediated through different regions of IL-8.

PMID: 7744810 The best characterized signal transduction pathway of G protein-coupled receptors starts with ligand binding, followed by activation of a heterotrimeric G protein.

PMID: 7744810 The best characterized signal transduction pathway of G protein-coupled receptors starts with ligand binding, followed by activation of a heterotrimeric G protein.

PMID: 7744810 An exchange occurs in the alpha subunit of the G protein from a GDP- to a GTP-bound state, resulting in a dissociation of the alpha subunit from the beta-gamma subunits

PMID: 7744810 An exchange occurs in the alpha subunit of the G protein from a GDP- to a GTP-bound state, resulting in a dissociation of the alpha subunit from the beta-gamma subunits

PMID: 7744810 The free alpha subunit can activate both phospholipase C (PLCbeta) 1 and PLCbeta2, whereas the free beta-gamma complex activates preferentially PLCbeta2.

PMID: 7744810 The free alpha subunit can activate both phospholipase C (PLCbeta) 1 and PLCbeta2, whereas the free beta-gamma complex activates preferentially PLCbeta2.

PMID: 7744810 The activation of PLCs results in hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two second messengers: inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DG).